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Results: 9
Inferring differential subcellular localisation in comparative spatial proteomics using BANDLE
Abstract The steady-state localisation of proteins provides vital insight into their function. These localisations are context specific with proteins translocating between different subcellular...
Oliver M Crook, Colin TR Davies, Lisa M Breckels, Josie A Christopher, Laurent Gatto, Paul DW Kirk, Kathryn S Lilley
Oct 10, 2022
A computational platform for high-throughput analysis of RNA sequences and modifications by mass spectrometry
Samuel Wein, Byron Andrews, Timo Sachsenberg, Helena Santos-Rosa, Oliver Kohlbacher, Tony Kouzarides, Benjamin A. Garcia, Hendrik Weisser
Feb 16, 2021
Abstract: The field of epitranscriptomics continues to reveal how post-transcriptional modification of RNA affects a wide variety of biological phenomena. A pivotal challenge in this area is the identification of modified RNA...
A method to disentangle and quantify host anabolic turnover in photosymbiotic holobionts with subcellular resolution
Abstract: A wide range of organisms host photosynthesizing symbionts. In these animals the metabolic exchange between host and symbionts has prevented in situ host anabolic turnover to be studied without the confounding effect...
Sequences in the cytoplasmic tail of SARS-CoV-2 Spike facilitate expression at the cell surface and syncytia formation
Jérôme Cattin-Ortolá, Lawrence G. Welch, Sarah L. Maslen, Guido Papa, Leo C. James, Sean Munro
Sep 09, 2021
Abstract: The Spike (S) protein of SARS-CoV-2 binds ACE2 to direct fusion with host cells. S comprises a large external domain, a transmembrane domain, and a short cytoplasmic tail. Understanding the intracellular trafficking of...
Extent of N-terminus exposure of monomeric alpha-synuclein determines its aggregation propensity
Amberley D. Stephens, Maria Zacharopoulou, Rani Moons, Giuliana Fusco, Neeleema Seetaloo, Anass Chiki, Philippa J. Woodhams, Ioanna Mela, Hilal A. Lashuel, Jonathan J. Phillips, Alfonso De Simone, Frank Sobott, Gabriele S. Kaminski Schierle
Jun 04, 2021
Abstract: As an intrinsically disordered protein, monomeric alpha-synuclein (aSyn) occupies a large conformational space. Certain conformations lead to aggregation prone and non-aggregation prone intermediates, but identifying...
Development and validation of an LC-MS/MS method for detection and quantification of in vivo derived metabolites of [Pyr 1 ]apelin-13 in humans
Duuamene Nyimanu, Richard G. Kay, Petra Sulentic, Rhoda E. Kuc, Philip Ambery, Lutz Jermutus, Frank Reimann, Fiona M. Gribble, Joseph Cheriyan, Janet J. Maguire, Anthony P. Davenport
Dec 26, 2020
Abstract: [Pyr1]apelin-13 is the predominant apelin peptide isoform in the human cardiovascular system and plasma. To date, few studies have investigated [Pyr1]apelin-13 metabolism in vivo in rats with no studies examining its...
A method to disentangle and quantify host anabolic turnover in photosymbiotic holobionts with subcellular resolution
Abstract: A wide range of organisms host photosynthesizing symbionts. In these animals the metabolic exchange between host and symbionts has prevented in situ host anabolic turnover to be studied without the confounding effect...
Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale
Martin Steger, Vadim Demichev, Mattias Backman, Uli Ohmayer, Phillip Ihmor, Stefan Müller, Markus Ralser, Henrik Daub
Sep 13, 2021
Abstract: Mass spectrometry (MS)-based ubiquitinomics provides system-level understanding of ubiquitin signaling. Here we present a scalable workflow for deep and precise in vivo ubiquitinome profiling, coupling an improved...
Hyperphosphorylated tau self-assembles into amorphous aggregates eliciting TLR4-dependent responses
Jonathan X Meng, Yu Zhang, Dominik Saman, Arshad M Haider, Suman De, Jason C Sang, Karen Brown, Kun Jiang, Jane Humphrey, Linda Julian, Eric Hidari, Steven F Lee, Gabriel Balmus, R Andres Floto, Clare Bryant, Justin LP Benesch, Yu Ye, David Klenerman
May 16, 2022