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Results: 20
Structure and efflux mechanism of the yeast pleiotropic drug resistance transporter Pdr5
Andrzej Harris, Manuel Wagner, Dijun Du, Stefanie Raschka, Lea-Marie Nentwig, Holger Gohlke, Sander H. J. Smits, Ben F. Luisi, Lutz Schmitt
Sep 08, 2021
Abstract: Pdr5, a member of the extensive ABC transporter superfamily, is representative of a clinically relevant subgroup involved in pleiotropic drug resistance. Pdr5 and its homologues drive drug efflux through uncoupled...
Structural insight into mitochondrial β-barrel outer membrane protein biogenesis
Kathryn A. Diederichs, Xiaodan Ni, Sarah E. Rollauer, Istvan Botos, Xiaofeng Tan, Martin S. King, Edmund R. S. Kunji, Jiansen Jiang, Susan K. Buchanan
Jul 05, 2021
Abstract: In mitochondria, β-barrel outer membrane proteins mediate protein import, metabolite transport, lipid transport, and biogenesis. The Sorting and Assembly Machinery (SAM) complex consists of three proteins that assemble...
A late-stage assembly checkpoint of the human mitochondrial ribosome large subunit.
Pedro Rebelo-Guiomar, Simone Pellegrino, Kyle C Dent, Aldema Sas-Chen, Leonor Miller-Fleming, Caterina Garone, Lindsey Van Haute, Jack F Rogan, Adam Dinan, Andrew Firth, Byron Andrews, Alex Whitworth, Schraga Schwartz, Alan Warren, Michal Minczuk
Feb 17, 2022
The structure of EXTL3 helps to explain the different roles of bi-domain exostosins in heparan sulfate synthesis
LFL Wilson, T Dendooven, SW Hardwick, A Echevarría-Poza, T Tryfona, KBRM Krogh, Dima Chirgadze, Ben Luisi, DT Logan, K Mani, P Dupree
Jun 09, 2022
Juxtaposition of Bub1 and Cdc20 on phosphorylated Mad1 during catalytic mitotic checkpoint complex assembly.
Elyse S Fischer, Conny WH Yu, Johannes F Hevler, Stephen H McLaughlin, Sarah L Maslen, Albert JR Heck, Stefan MV Freund, David Barford
Oct 27, 2022
In response to improper kinetochore-microtubule attachments in mitosis, the spindle assembly checkpoint (SAC) assembles the mitotic checkpoint complex (MCC) to inhibit the anaphase-promoting complex/cyclosome, thereby delaying...
MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography
Max T. B. Clabbers, Susannah Holmes, Timothy W. Muusse, Parimala R. Vajjhala, Sara J. Thygesen, Alpeshkumar K. Malde, Dominic J. B. Hunter, Tristan I. Croll, Leonie Flueckiger, Jeffrey D. Nanson, Md. Habibur Rahaman, Andrew Aquila, Mark S. Hunter, Mengning Liang, Chun Hong Yoon, Jingjing Zhao, Nadia A. Zatsepin, Brian Abbey, Emma Sierecki, Yann Gambin, Katryn J. Stacey, Connie Darmanin, Bostjan Kobe, Hongyi Xu, Thomas Ve
May 10, 2021
Abstract: MyD88 and MAL are Toll-like receptor (TLR) adaptors that signal to induce pro-inflammatory cytokine production. We previously observed that the TIR domain of MAL (MALTIR) forms filaments in vitro and induces formation...
Age-dependent formation of TMEM106B amyloid filaments in human brains.
Manuel Schweighauser, Diana Arseni, Mehtap Bacioglu, Melissa Huang, Sofia Lövestam, Yang Shi, Yang Yang, Wenjuan Zhang, Abhay Kotecha, Holly J Garringer, Ruben Vidal, Grace I Hallinan, Kathy L Newell, Airi Tarutani, Shigeo Murayama, Masayuki Miyazaki, Yuko Saito, Mari Yoshida, Kazuko Hasegawa, Tammaryn Lashley, Tamas Revesz, Gabor G Kovacs, John van Swieten, Masaki Takao, Masato Hasegawa, Bernardino Ghetti, Maria Grazia Spillantini, Benjamin Ryskeldi-Falcon, Alexey G Murzin, Michel Goedert, Sjors HW Scheres
May 11, 2022
Many age-dependent neurodegenerative diseases, such as Alzheimer's and Parkinson's, are characterized by abundant inclusions of amyloid filaments. Filamentous inclusions of the proteins tau, amyloid-β, α-synuclein and...
The MiDAC histone deacetylase complex is essential for embryonic development and has a unique multivalent structure
Robert E. Turnbull, Louise Fairall, Almutasem Saleh, Emma Kelsall, Kyle L. Morris, T. J. Ragan, Christos G. Savva, Aditya Chandru, Christopher J. Millard, Olga V. Makarova, Corinne J. Smith, Alan M. Roseman, Andrew M. Fry, Shaun M. Cowley, John W. R. Schwabe
Jun 26, 2020
Abstract: MiDAC is one of seven distinct, large multi-protein complexes that recruit class I histone deacetylases to the genome to regulate gene expression. Despite implications of involvement in cell cycle regulation and in...
Structure of inhibitor-bound mammalian complex I
Hannah R. Bridges, Justin G. Fedor, James N. Blaza, Andrea Di Luca, Alexander Jussupow, Owen D. Jarman, John J. Wright, Ahmed-Noor A. Agip, Ana P. Gamiz-Hernandez, Maxie M. Roessler, Ville R. I. Kaila, Judy Hirst
Oct 18, 2021
Mechanisms underlying TARP modulation of the GluA1/2-γ8 AMPA receptor.
Beatriz Herguedas, Bianka K Kohegyi, Jan-Niklas Dohrke, Jake F Watson, Danyang Zhang, Hinze Ho, Saher A Shaikh, Remigijus Lape, James M Krieger, Ingo H Greger
Feb 09, 2022
AMPA-type glutamate receptors (AMPARs) mediate rapid signal transmission at excitatory synapses in the brain. Glutamate binding to the receptor's ligand-binding domains (LBDs) leads to ion channel activation and desensitization....
Mechanisms of inhibition and activation of extrasynaptic αβ GABAA receptors.
Vikram Babu Kasaragod, Martin Mortensen, Steven Hardwick, Ayla Wahid, Valentina Dorovykh, Dima Chirgadze, Trevor G Smart, Paul Miller
Feb 16, 2022
Higher-order phosphatase–substrate contacts terminate the integrated stress response
Abstract: Many regulatory PPP1R subunits join few catalytic PP1c subunits to mediate phosphoserine and phosphothreonine dephosphorylation in metazoans. Regulatory subunits engage the surface of PP1c, locally affecting flexible...
The MiDAC histone deacetylase complex is essential for embryonic development and has a unique multivalent structure
Robert E. Turnbull, Louise Fairall, Almutasem Saleh, Emma Kelsall, Kyle L. Morris, T. J. Ragan, Christos G. Savva, Aditya Chandru, Christopher J. Millard, Olga V. Makarova, Corinne J. Smith, Alan M. Roseman, Andrew M. Fry, Shaun M. Cowley, John W. R. Schwabe
Jun 26, 2021
Abstract: MiDAC is one of seven distinct, large multi-protein complexes that recruit class I histone deacetylases to the genome to regulate gene expression. Despite implications of involvement in cell cycle regulation and in...
Structure of inhibitor-bound mammalian complex I
Hannah R. Bridges, Justin G. Fedor, James N. Blaza, Andrea Di Luca, Alexander Jussupow, Owen D. Jarman, John J. Wright, Ahmed-Noor A. Agip, Ana P. Gamiz-Hernandez, Maxie M. Roessler, Ville R. I. Kaila, Judy Hirst
Oct 16, 2020
Structure and dynamics of Toll immunoreceptor activation in the mosquito Aedes aegypti.
Yoann Saucereau, Thomas H Wilson, Matthew CK Tang, Martin C Moncrieffe, Steven W Hardwick, Dimitri Y Chirgadze, Sandro G Soares, Maria Jose Marcaida, Nicholas J Gay, Monique Gangloff
Sep 02, 2022
Aedes aegypti has evolved to become an efficient vector for arboviruses but the mechanisms of host-pathogen tolerance are unknown. Immunoreceptor Toll and its ligand Spaetzle have undergone duplication which may allow...
Structural insight into mitochondrial β-barrel outer membrane protein biogenesis
Kathryn A. Diederichs, Xiaodan Ni, Sarah E. Rollauer, Istvan Botos, Xiaofeng Tan, Martin S. King, Edmund R. S. Kunji, Jiansen Jiang, Susan K. Buchanan
Jul 03, 2020
Abstract: In mitochondria, β-barrel outer membrane proteins mediate protein import, metabolite transport, lipid transport, and biogenesis. The Sorting and Assembly Machinery (SAM) complex consists of three proteins that assemble...
Cryo-EM reveals the architecture of the PELP1-WDR18 molecular scaffold.
Jacob Gordon, Fleur L Chapus, Elizabeth G Viverette, Jason G Williams, Leesa J Deterding, Juno M Krahn, Mario J Borgnia, Joseph Rodriguez, Alan J Warren, Robin E Stanley
Nov 13, 2022
Donor-strand exchange drives assembly of the TasA scaffold in Bacillus subtilis biofilms.
Jan Böhning, Mnar Ghrayeb, Conrado Pedebos, Daniel K Abbas, Syma Khalid, Liraz Chai, Tanmay AM Bharat
Nov 19, 2022
Many bacteria in nature exist in multicellular communities termed biofilms, where cells are embedded in an extracellular matrix that provides rigidity to the biofilm and protects cells from chemical and mechanical stresses. In...