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Results: 20
Structural insights into the EthR-DNA interaction using native mass spectrometry
DSH Chan, WG Seetoh, BN McConnell, D Matak-Vinković, SE Thomas, V Mendes, M Blaszczyk, AG Coyne, TL Blundell, C Abell
Mar 09, 2017
Enhanced detection of early hepatocellular carcinoma by serum SELDI-TOF proteomic signature combined with alpha-fetoprotein marker.
Lei Chen, David WY Ho, Nikki PY Lee, Stella Sun, Brian Lam, Kwong-Fai Wong, Xin Yi, George K Lau, Eddy WY Ng, Terence CW Poon, Paul BS Lai, Zongwei Cai, Jirun Peng, Xisheng Leng, Ronnie TP Poon, John M Luk
Mar 08, 2021
BACKGROUND: Biomarkers for accurate diagnosis of early hepatocellular carcinoma (HCC) are limited in number and clinical validation. We applied SELDI-TOF-MS ProteinChip technology to identify serum profile for distinguishing HCC...
A multidrug ABC transporter with a taste for salt.
Saroj Velamakanni, Calvin HF Lau, Daniel AP Gutmann, Henrietta Venter, Nelson P Barrera, Markus A Seeger, Barbara Woebking, Dijana Matak-Vinkovic, Lekshmy Balakrishnan, Yao Yao, Edmond CY U, Richard A Shilling, Carol V Robinson, Peter Thorn, Hendrik W van Veen
Oct 05, 2020
BACKGROUND: LmrA is a multidrug ATP-binding cassette (ABC) transporter from Lactococcus lactis with no known physiological substrate, which can transport a wide range of chemotherapeutic agents and toxins from the cell. The...
The recognition of collagen and triple-helical toolkit peptides by MMP-13
Joanna-Marie Howes, Dominique Bihan, David A Slatter, Samir W Hamaia, Len C Packman, Vera Knauper, Robert Visse, Richard W Farndale
Jul 17, 2014
Collagen Collagenolysis Hemopexin Domain MMP-13 Matrix Metalloproteinase (MMP) Protease Protein Conformation Substrate Specificity Toolkit Peptides Amino Acid Sequence Base Sequence Collagen Type II DNA Primers Matrix Metalloproteinase 13 Molecular Sequence Data Peptides Proteolysis Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Substrate Specificity
Action of an endo-β-1,3(4)-glucanase on cellobiosyl unit structure in barley β-1,3
Takao Kuge, Hiroki Nagoya, Theodora Tryfona, Tsunemi Kurokawa, Yoshihisa Yoshimi, Naoshi Dohmae, Kazufumi Tsubaki, Paul Dupree, Yoichi Tsumuraya, Toshihisa Kotake
Sep 02, 2015
Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase.
Leila Lo Leggio, Thomas J Simmons, Jens-Christian N Poulsen, Kristian EH Frandsen, Glyn R Hemsworth, Mary A Stringer, Pernille von Freiesleben, Morten Tovborg, Katja S Johansen, Leonardo De Maria, Paul V Harris, Chee-Leong Soong, Paul Dupree, Theodora Tryfona, Nicolas Lenfant, Bernard Henrissat, Gideon J Davies, Paul H Walton
Jul 24, 2015
Acids Catalytic Domain Cellulose Copper Crystallography X-Ray Electron Spin Resonance Spectroscopy evolution Molecular fungi Genomics Histidine Maltose Mixed Function Oxygenases Oligosaccharides Oxygen Phylogeny Polysaccharides Protein Conformation Protein Structure Tertiary Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Starch Substrate Specificity beta-Amylase
Organization of Subunits in the Membrane Domain of the Bovine F-ATPase Revealed by Covalent Cross-linking.
Jennifer Lee, ShuJing Ding, Thomas B Walpole, Andrew N Holding, Martin G Montgomery, Ian M Fearnley, John E Walker
Aug 11, 2017
ATP synthase membrane energetics Mitochondria Protein Structure Protein-Protein Interaction supernumerary membrane subunits topography Amino Acid Sequence Animals Catalytic Domain Cattle Cell Membrane Cross-Linking Reagents Mitochondria Mitochondrial Proton-Translocating ATPases Models Molecular Molecular Sequence Data Mutation Peptide Fragments Protein Conformation Protein Structure Secondary Protein Subunits Sequence Homology Amino Acid Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization
Purification of Recombinant α-synuclein
Amberley D Stephens, Dijana Matak-Vinkovic, Ana Fernandez-Villegas, Gabriele S Kaminski Schierle
Nov 23, 2020
The initial state of the intrinsically disordered protein α-synuclein (aSyn), e.g., the presence of oligomers and degradation products, or the presence of contaminants and adducts can greatly influence the aggregation kinetics...
Cell Line Cell Survival Chemical Precipitation Chromatography Gel Chromatography Ion Exchange Chromatography Liquid Escherichia coli Humans Intrinsically Disordered Proteins Microscopy Electron Transmission Protein Aggregates Protein Conformation Protein Conformation beta-Strand Recombinant Proteins Spectrometry Mass Electrospray Ionization ALPHA-SYNUCLEIN
The pentameric nucleoplasmin fold is present in Drosophila FKBP39 and a large number of chromatin-related proteins.
Christian Edlich-Muth, Jean-Baptiste Artero, Phil Callow, Marcin R Przewloka, Aleksandra A Watson, Wei Zhang, David M Glover, Janusz Debski, Michal Dadlez, Adam R Round, V Trevor Forsyth, Ernest D Laue
May 21, 2015
FKBP NMR histone chaperone nucleoplasmin structure determination Amino Acid Sequence Animals Arabidopsis Chromatin Cross-Linking Reagents Crystallography X-Ray Drosophila Proteins Drosophila melanogaster Histone Chaperones Histone Deacetylase 2 Histones Immunoprecipitation Models Molecular Molecular Sequence Data Nucleoplasmins Phylogeny Protein Binding Protein Conformation Protein Structure Tertiary Recombinant Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sequence Homology Amino Acid Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Structure-Activity Relationship Tacrolimus Binding Proteins
Lipid zonation and phospholipid remodeling in nonalcoholic fatty liver disease.
Zoe Hall, Nicholas J Bond, Tom Ashmore, Francis Sanders, Zsuzsanna Ament, Xinzhu Wang, Andrew J Murray, Elena Bellafante, Sam Virtue, Antonio Vidal-Puig, Michael Allison, Susan E Davies, Albert Koulman, Michele Vacca, Julian L Griffin
Feb 08, 2017
Animals Biopsy Needle Diet High-Fat Diet Western Disease Models Animal Eicosanoids Fatty Liver Humans Immunohistochemistry Liver Cirrhosis Liver Neoplasms Liver Regeneration Male Mass Spectrometry Mice Mice Inbred C57BL non-alcoholic fatty liver disease Phospholipids Prognosis Random Allocation Risk Assessment Severity of Illness Index Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization
A How to Guide
Applying MALDI-ToF mass spectrometry as a clinical diagnostic test for viruses is different from that of bacteria, fungi and other micro-organisms. This is because the systems biology of viral infections, the size and chemical...
Colocalization Features for Classification of Tumors Using Desorption Electrospray Ionization Mass Spectrometry Imaging.
Supervised modeling of mass spectrometry imaging (MSI) data is a crucial component for the detection of the distinct molecular characteristics of cancerous tissues. Currently, two types of supervised analyses are mainly used on...
Photo-induced halide redistribution in organic-inorganic perovskite films.
Dane W deQuilettes, Wei Zhang, Victor M Burlakov, Daniel J Graham, Tomas Leijtens, Anna Osherov, Vladimir Bulović, Henry J Snaith, David S Ginger, Samuel D Stranks
Aug 14, 2017
Organic-inorganic perovskites such as CH3NH3PbI3 are promising materials for a variety of optoelectronic applications, with certified power conversion efficiencies in solar cells already exceeding 21%. Nevertheless...
A randomised controlled trial of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDITOF-MS) versus conventional microbiological methods for identifying pathogens
Behzad Nadjm, Vu Quoc Dat, James I Campbell, Vu Tien Viet Dung, Alessandro Torre, Nguyen Thi Cam Tu, Ninh Thi Thanh Van, Dao Tuyet Trinh, Nguyen Phu Huong Lan, Nguyen Vu Trung, Nguyen Thi Thuy Hang, Le Thi Hoi, Stephen Baker, Marcel Wolbers, Nguyen Van Vinh Chau, Nguyen Van Kinh, Guy E Thwaites, H Rogier van Doorn, Heiman FL Wertheim
Apr 18, 2019
OBJECTIVES: We assessed the impact of MALDITOF-MS on the timeliness of optimal antimicrobial therapy through a parallel-arm randomised controlled trial in two hospitals in Vietnam. METHODS: We recruited patients with a pathogen...
antibacterial agents bacteraemia Matrix-assisted laser desorption-ionization mass spectrometry Microbiological Techniques Vietnam Adult Anti-Bacterial Agents Antifungal Agents Bacteremia Bacteria Female fungi Humans Male Microbiological Techniques Middle Aged Mycoses Prospective Studies Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Time Factors Treatment Outcome Vietnam
Myc Expression Drives Aberrant Lipid Metabolism in Lung Cancer
Z Hall, Z Ament, CH Wilson, DL Burkhart, T Ashmore, A Koulman, T Littlewood, GI Evan, JL Griffin
Dec 05, 2016
Adenocarcinoma Adenocarcinoma of Lung Animals Disease Models Animal Eicosanoids Immunohistochemistry Lipid Metabolism Lung Neoplasms Mass Spectrometry Mice Mice Transgenic Polymerase Chain Reaction Proto-Oncogene Proteins c-myc Proto-Oncogene Proteins p21(ras) Signal Transduction Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization
Conservation of complete trimethylation of lysine-43 in the rotor ring of c-subunits of metazoan adenosine triphosphate (ATP) synthases.
Thomas B Walpole, David N Palmer, Huibing Jiang, ShuJing Ding, Ian M Fearnley, John E Walker
Aug 11, 2017
The rotors of ATP synthases turn about 100 times every second. One essential component of the rotor is a ring of hydrophobic c-subunits in the membrane domain of the enzyme. The rotation of these c-rings is driven by a...
Characterisation of a natural variant of the γ-butyrolactone signalling receptor.
Amino Acid Sequence Amino Acids Anti-Bacterial Agents Bacterial Proteins Base Sequence Chymotrypsin DNA Bacterial Electrophoresis Gel Two-Dimensional Electrophoretic Mobility Shift Assay Escherichia coli Gene Expression Regulation Bacterial Kanamycin Molecular Sequence Data Mutation Point Mutation Promoter Regions Genetic Protein Binding Protein Structure Tertiary Receptors GABA-A Reproducibility of Results Sequence Alignment Signal Transduction Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Streptomyces coelicolor Transcription Genetic
Mannosidase 2, alpha 1 deficiency is associated with ricin resistance in embryonic stem (ES) cells.
Wei Wang, Christine Hale, Dave Goulding, Stuart M Haslam, Bérangère Tissot, Christopher Lindsay, Stephen Michell, Rick Titball, Jun Yu, Ana Luisa Toribio, Raffaella Rossi, Anne Dell, Allan Bradley, Gordon Dougan
Oct 14, 2020
Host gene products required for mediating the action of toxins are potential targets for reversing or controlling their pathogenic impact following exposure. To identify such targets libraries of insertional gene-trap mutations...
Using Neutron Reflectometry to Discern the Structure of Fibrinogen Adsorption at the Stainless Steel/Aqueous Interface.