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Animals CRISPR-Cas Systems Gene Knockout Techniques Genetic Vectors Intestine Small Mice Organ Culture Techniques Organoids RNA Guide Kinetoplastida Wnt Signaling Pathway

CRISPR-Cas Systems RNA Guide CRISPR-Cas Systems DNA Cleavage RNA DNA Bacterial Proteins

CRISPR-Cas9 technology has accelerated biological research becoming routine for many laboratories. It is rapidly replacing conventional gene editing techniques and has high utility for both genome-wide and gene-focussed...

Animals CRISPR-Cas Systems GPI-Linked Proteins Gene Editing Gene Library Genetic Vectors Genome-Wide Association Study Humans Lentivirus Mice Phenotype RNA Guide Kinetoplastida Signal Transduction

CRISPR-Associated Proteins CRISPR-Cas Systems Clustered Regularly Interspaced Short Palindromic Repeats Crystallography X-Ray Escherichia coli Escherichia coli Proteins RNA Editing RNA Bacterial RNA Guide Kinetoplastida

Alleles Base Sequence CRISPR-Cas Systems Cell Line Deoxyribonuclease I Drug Discovery Gene Editing Gene Knockout Techniques Gene Order Gene Targeting Genetic Loci Genetic Vectors Genomics Genotype High-Throughput Screening Assays Humans Mutagenesis Phenotype RNA Guide Kinetoplastida Vascular Endothelial Growth Factor A

Hundreds of human genes are associated with neurological diseases, but translation into tractable biological mechanisms is lagging. Larval zebrafish are an attractive model to investigate genetic contributions to neurological...

CRISPR G0 Behaviour Circadian Rhythm Genetics Genomics knockout neuroscience Sleep Zebrafish Animals Behavior Animal CRISPR-Cas Systems Embryo Nonmammalian Gene Knockout Techniques Genetic Testing Phenotype RNA Guide Kinetoplastida Zebrafish

Cas12a is a programmable nuclease for adaptive immunity against invading nucleic acids in CRISPR-Cas systems. Here, we report the crystal structures of apo Cas12a from Lachnospiraceae bacterium MA2020 (Lb2) and the...

Bacterial Proteins DNA RNA DNA Cleavage CRISPR-Cas Systems RNA Guide CRISPR-Cas Systems

In recent years mitochondrial DNA (mtDNA) has transitioned to greater prominence across diverse areas of biology and medicine. The recognition of mitochondria as a major biochemical hub, contributions of mitochondrial...

CRISPR/Cas9 RNA import mitoTALEN Mitochondria mtDNA mtZFN Animals Biolistics Biological Transport CRISPR-Cas Systems DNA Mitochondrial Dependovirus Endodeoxyribonucleases Gene Editing Genetic Vectors Genome Mitochondrial Mammals Mitochondria Polyribonucleotide Nucleotidyltransferase RNA Guide Kinetoplastida Transcription Activator-Like Effector Nucleases Transcription Factors