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Chemokine (C-C Motif) Receptor 2 Mediates Dendritic Cell Recruitment to the Human Colon but Is Not Responsible for Differences Observed in Dendritic Cell Subsets, Phenotype, and Function Between the Proximal and Distal Colon.
David Bernardo, Lydia Durant, Elizabeth R Mann, Elizabeth Bassity, Enrique Montalvillo, Ripple Man, Rakesh Vora, Durga Reddi, Fahri Bayiroglu, Luis Fernández-Salazar, Nick R English, Simon TC Peake, Jon Landy, Gui H Lee, George Malietzis, Yi Harn Siaw, Aravinth U Murugananthan, Phil Hendy, Eva Sánchez-Recio, Robin KS Phillips, Jose A Garrote, Paul Scott, Julian Parkhill, Malte Paulsen, Ailsa L Hart, Hafid O Al-Hassi, Eduardo Arranz, Alan W Walker, Simon R Carding, Stella C Knight
Sep 06, 2019
BACKGROUND & AIMS: Most knowledge about gastrointestinal (GI)-tract dendritic cells (DC) relies on murine studies where CD103+ DC specialize in generating immune tolerance with the functionality of CD11b+/- subsets being...
AMOVA analysis of molecular variance CCL chemokine (C-C motif) ligand CCR chemokine (C-C motif) receptor CCR2 CFSE 5-carboxy fluorescein diacetate succinimidyl ester DC dendritic cells DL detection limit Dendritic Cells Distal colon FACS fluorescence-activated cell sorting FITC fluorescein isothiocyanate GI Gastrointestinal Human Gastrointestinal Tract IL Interleukin ILT3 Ig-like transcript 3 LPMC lamina propria mononuclear cells Microbiota Mφ macrophages PBMC peripheral blood mononuclear cells pcr polymerase chain reaction Proximal Colon RALDH2 retinaldehyde dehydrogenase type 2 SIRPα signal regulatory protein α SPB sodium phosphate buffer Treg regulatory T-cells mDC myeloid dendritic cell pDC plasmacytoid dendritic cell
Autophagy plays an important role in protecting Pacific oysters from OsHV-1 and Vibrio aestuarianus infections.
Pierrick Moreau, Kevin Moreau, Amélie Segarra, Delphine Tourbiez, Marie-Agnès Travers, David C Rubinsztein, Tristan Renault
May 15, 2015
Autophagy Crassostrea gigas OsHV-1 Pacific oyster Vibrio aestuarianus ATG autophagy-related Atg8–PE Atg8–phosphatidylethenolamine DNA deoxyribonucleic acid hpi hours postinfection LC3-II cleaved lipidated and autophagosome-associated form of LC3 MAP1LC3A/B (LC3A/B) microtubule-associated proteins 1 light chain 3 alpha/beta (mammalian orthologs of the predicted Crassostrea gigas LC3 and yeast Atg8) NH4Cl ammonium chloride OsHV-1 Ostreid herpesvirus 1 pcr polymerase chain reaction