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Mechanical design principles of a mitotic spindle.
An organised spindle is crucial to the fidelity of chromosome segregation, but the relationship between spindle structure and function is not well understood in any cell type. The anaphase B spindle in fission yeast has a...
Mouse model of chromosome mosaicism reveals lineage-specific depletion of aneuploid cells and normal developmental potential.
Helen Bolton, Sarah JL Graham, Niels Van der Aa, Parveen Kumar, Koen Theunis, Elia Fernandez Gallardo, Thierry Voet, Magdalena Zernicka-Goetz
Mar 30, 2016
Aneuploidy Animals Blastocyst Cell Count Cell Lineage Chromosome Segregation Chromosomes Mammalian Embryo Implantation Female Fertilization in Vitro Humans In Situ Hybridization Fluorescence Male Mice Mice Inbred C57BL Mice Inbred CBA Mitosis Models Genetic Morpholines Mosaicism Protein Kinase Inhibitors Purines Zygote
Trypanosome KKIP1 Dynamically Links the Inner Kinetochore to a Kinetoplastid Outer Kinetochore Complex.
Kinetochores perform an essential role in eukaryotes, coupling chromosomes to the mitotic spindle. In model organisms they are composed of a centromere-proximal inner kinetochore and an outer kinetochore network that binds to...
Non-SMC condensin I complex proteins control chromosome segregation and survival of proliferating cells in the zebrafish neural retina.
Sabine Seipold, Florian C Priller, Paul Goldsmith, William A Harris, Herwig Baier, Salim Abdelilah-Seyfried
Jun 13, 2011
Meiotic crossover reduction by virus-induced gene silencing enables the efficient generation of chromosome substitution lines and reverse breeding in Arabidopsis thaliana.
Vanesa Calvo-Baltanás, Cris L Wijnen, Chao Yang, Nina Lukhovitskaya, C Bastiaan de Snoo, Linus Hohenwarter, Joost JB Keurentjes, Hans de Jong, Arp Schnittger, Erik Wijnker
Oct 26, 2020
Plant breeding applications exploiting meiotic mutant phenotypes (like the increase or decrease of crossover (CO) recombination) have been proposed over the last years. As recessive meiotic mutations in breeding lines may affect...
Arabidopsis thaliana MSH5 chromosome substitution lines Meiosis reverse breeding technical advance virus-induced gene silencing Arabidopsis Arabidopsis Proteins Chimera Chromosome Segregation Chromosomes Plant Crossing Over Genetic DNA-Binding Proteins Fertility Gene Expression Regulation Plant Gene Silencing Meiosis Mutation Phenotype plant breeding Transgenes
Repurposing of synaptonemal complex proteins for kinetochores in Kinetoplastida.
Chromosome segregation in eukaryotes is driven by the kinetochore, a macromolecular complex that connects centromeric DNA to microtubules of the spindle apparatus. Kinetochores in well-studied model eukaryotes consist of a core...
Cell-based screen for altered nuclear phenotypes reveals senescence progression in polyploid cells after Aurora kinase B inhibition.
Mahito Sadaie, Christian Dillon, Masako Narita, Andrew RJ Young, Claire J Cairney, Lauren S Godwin, Christopher J Torrance, Dorothy C Bennett, W Nicol Keith, Masashi Narita
Jun 30, 2015
Control of Chromosome Segregation by the Aurora B Complex
Srinath Cidambi Sampath
Jan 01, 0001
Chromosome segregation during cell division requires spindle assembly around M-phase chromatin. In cells lacking centrosomes, such as those found in female meiosis, chromosomes themselves nucleate and stabilize microtubules in...
Chromosome Segregation chromosomal passenger complex CPC activation Aurora B spindle assembly Life Sciences
Published by: Rockefeller University
Developmental potential of aneuploid human embryos cultured beyond implantation.
Marta N Shahbazi, Tianren Wang, Xin Tao, Bailey AT Weatherbee, Li Sun, Yiping Zhan, Laura Keller, Gary D Smith, Antonio Pellicer, Richard T Scott, Emre Seli, Magdalena Zernicka-Goetz
Jul 20, 2020
Aneuploidy, the presence of an abnormal number of chromosomes, is a major cause of early pregnancy loss in humans. Yet, the developmental consequences of specific aneuploidies remain unexplored. Here, we determine the extent of...
Aneuploidy Antigens CD Cadherins Cell Adhesion Cell Cycle Checkpoints Cell Lineage Chromosome Segregation Chromosomes Human Pair 16 Chromosomes Human Pair 21 Embryo Implantation Embryo Mammalian Embryonic Development Female Genes erbB-1 Genetic Testing Humans Monosomy Mosaicism Pregnancy Stem Cells Trisomy
Centrosome function is critical during terminal erythroid differentiation.
Red blood cells are produced by terminal erythroid differentiation, which involves the dramatic morphological transformation of erythroblasts into enucleated reticulocytes. Microtubules are important for enucleation, but it is...
Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.
David Virant, Ilijana Vojnovic, Jannik Winkelmeier, Marc Endesfelder, Bartosz Turkowyd, David Lando, Ulrike Endesfelder
Feb 26, 2023
Molecular mechanism of Mad1 kinetochore targeting by phosphorylated Bub1.
Elyse S Fischer, Conny WH Yu, Dom Bellini, Stephen H McLaughlin, Christian M Orr, Armin Wagner, Stefan MV Freund, David Barford
May 20, 2021
A computational model of the early stages of acentriolar meiotic spindle assembly.
Gaelle Letort, Isma Bennabi, Serge Dmitrieff, François Nedelec, Marie-Hélène Verlhac, Marie-Emilie Terret
Jul 30, 2020
The mitotic spindle is an ensemble of microtubules responsible for the repartition of the chromosomal content between the two daughter cells during division. In metazoans, spindle assembly is a gradual process involving dynamic...
Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation.
Duccio Conti, Parveen Gul, Asifa Islam, José M Martín-Durán, Richard W Pickersgill, Viji M Draviam
Dec 25, 2019
Cell Biology CHROMOSOME human kinetochore microtubule Mitosis Alcian Blue Aurora Kinase B Chromosomal Proteins Non-Histone Chromosome Segregation Cytoskeletal Proteins HeLa Cells Humans Kinetochores Metaphase Microtubule-Associated Proteins Microtubules Molecular Docking Simulation Phenazines phenothiazines Protein Conformation Protein Interaction Domains and Motifs Receptors Neuropeptide Y Resorcinols
Molecular Insights into Division of Single Human Cancer Cells in On-Chip Transparent Microtubes.
Wang Xi, Christine K Schmidt, Samuel Sanchez, David H Gracias, Rafael E Carazo-Salas, Richard Butler, Nicola Lawrence, Stephen P Jackson, Oliver G Schmidt
Jun 24, 2016
Oncogenic KRAS triggers MAPK-dependent errors in mitosis and MYC-dependent sensitivity to anti-mitotic agents.
Active Transport Cell Nucleus Antineoplastic Agents Cell Line Tumor Cell Nucleus Chromosome Segregation HCT116 Cells HeLa Cells Humans Microscopy Confocal Mitogen-Activated Protein Kinases Mitosis Mutation Proto-Oncogene Proteins c-myc Proto-Oncogene Proteins p21(ras) RNA Interference Time-Lapse Imaging
Counteraction between Astrin-PP1 and Cyclin-B-CDK1 pathways protects chromosome-microtubule attachments independent of biorientation.
Defects in chromosome-microtubule attachment can cause chromosomal instability (CIN), frequently associated with infertility and aggressive cancers. Chromosome-microtubule attachment is mediated by a large macromolecular...
Regulation of Chromosome Segregation by the Chromosomal Passenger Complex
Boo Shan Tseng
Jan 01, 0001
Life depends on faithful DNA segregation. The molecular underpinnings controlling this segregation are not fully understood. Here I describe the role of the chromosomal passenger complex (CPC) in the regulation of chromosome...
Chromosome Segregation chromosomal passenger complex Aurora B spindle assembly xenopus egg Life Sciences
Published by: Rockefeller University
Detection of mutations in KLHL3 and CUL3 in families with FHHt (familial hyperkalaemic hypertension or Gordon's syndrome).
Mark Glover, James S Ware, Amanda Henry, Martin Wolley, Roddy Walsh, Louise V Wain, Shengxin Xu, William G Van't Hoff, Martin D Tobin, Ian P Hall, Stuart Cook, Richard D Gordon, Michael Stowasser, Kevin M O'Shaughnessy
Aug 19, 2014
A cryptic hydrophobic pocket in the polo-box domain of the polo-like kinase PLK1 regulates substrate recognition and mitotic chromosome segregation.
Pooja Sharma, Robert Mahen, Maxim Rossmann, Jamie E Stokes, Bryn Hardwick, David J Huggins, Amy Emery, Dominique L Kunciw, Marko Hyvönen, David R Spring, Grahame J McKenzie, Ashok R Venkitaraman
Aug 21, 2019
3' Untranslated Regions Cell Cycle Proteins Cell Survival Chromosome Segregation HeLa Cells Histones Humans Kinetochores Mitosis Mutagenesis Protein Binding Protein Domains Protein Kinase Inhibitors Protein Serine-Threonine Kinases Protein Structure Tertiary Proto-Oncogene Proteins RNA Interference RNA Small Interfering Substrate Specificity
The Cdc14 Phosphatase Controls Resolution of Recombination Intermediates and Crossover Formation during Meiosis.
Paula Alonso-Ramos, David Álvarez-Melo, Katerina Strouhalova, Carolina Pascual-Silva, George B Garside, Meret Arter, Teresa Bermejo, Rokas Grigaitis, Rahel Wettstein, Marta Fernández-Díaz, Joao Matos, Marco Geymonat, Pedro A San-Segundo, Jesús A Carballo
Sep 13, 2021
Meiotic defects derived from incorrect DNA repair during gametogenesis can lead to mutations, aneuploidies and infertility. The coordinated resolution of meiotic recombination intermediates is required for crossover formation...
CDK1 Cdc14 Cdc20 Cdc5 Holliday junction Mus81 Ndt80 Sgs1 Yen1 Aneuploidy meiotic recombination CDC2 Protein Kinase Cell Cycle Proteins Chromosome Segregation Crossing Over Genetic DNA Repair DNA Cruciform Gametogenesis Holliday Junction Resolvases homologous recombination Meiosis Mutation Phosphorylation Protein Tyrosine Phosphatases Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins
Evidence that polyploidy in esophageal adenocarcinoma originates from mitotic slippage caused by defective chromosome attachments.
Stacey J Scott, Xiaodun Li, Sriganesh Jammula, Ginny Devonshire, Catherine Lindon, Rebecca C Fitzgerald, Pier Paolo D'Avino
Jan 29, 2021
Polyploidy is present in many cancer types and is increasingly recognized as an important factor in promoting chromosomal instability, genome evolution, and heterogeneity in cancer cells. However, the mechanisms that trigger...
F-Actin Interactome Reveals Vimentin as a Key Regulator of Actin Organization and Cell Mechanics in Mitosis.
Murielle P Serres, Matthias Samwer, Binh An Truong Quang, Geneviève Lavoie, Upamali Perera, Dirk Görlich, Guillaume Charras, Mark Petronczki, Philippe P Roux, Ewa K Paluch
Jul 02, 2020
Most metazoan cells entering mitosis undergo characteristic rounding, which is important for accurate spindle positioning and chromosome separation. Rounding is driven by contractile tension generated by myosin motors in the...