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Single-Cell Biochemical Multiplexing by Multidimensional Phasor Demixing and Spectral Fluorescence Lifetime Imaging Microscopy
Kalina T. Haas
,
Maximilian W. Fries
,
Ashok R. Venkitaraman
,
Alessandro Esposito
Jun 10, 2021
Revealing mechanisms underpinning cell function requires understanding the relationship between different biochemical reactions in living cells. However, our capabilities to monitor more than two biochemical reactions in living...
Physics
sFLIM
FRET biosensors
TCSPC
spectral demixing
biochemical multiplexing
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