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The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells.
Hebin Liu, Helga Schneider, Asha Recino, Christine Richardson, Martin W Goldberg, Christopher E Rudd
Aug 18, 2015
Active Transport Cell Nucleus Adaptor Proteins Signal Transducing Animals Cell Line GTPase-Activating Proteins Humans Jurkat Cells Mice Microscopy Electron Transmission NFATC Transcription Factors Nuclear Pore Phosphoproteins Protein Binding Small Ubiquitin-Related Modifier Proteins T-Lymphocytes Transcription Factor RelA
Interferon-β-induced miR-1 alleviates toxic protein accumulation by controlling autophagy.
Camilla Nehammer, Patrick Ejlerskov, Sandeep Gopal, Ava Handley, Leelee Ng, Pedro Moreira, Huikyong Lee, Shohreh Issazadeh-Navikas, David C Rubinsztein, Roger Pocock
Dec 10, 2019
Appropriate regulation of autophagy is crucial for clearing toxic proteins from cells. Defective autophagy results in accumulation of toxic protein aggregates that detrimentally affect cellular function and organismal survival....
C. elegans Autophagy Biochemistry chemical biology Genetics Genomics human cells mammalian cells microRNA protein aggregation 3' Untranslated Regions Animals Autophagy Base Sequence Caenorhabditis elegans Caenorhabditis elegans Proteins GTPase-Activating Proteins HeLa Cells Humans Huntingtin Protein Interferon-beta Mice MicroRNAs Mutant Proteins Peptides Protein Aggregates RNA Messenger rab GTP-Binding Proteins rab7 GTP-Binding Proteins
Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29.
Harriet Crawley-Snowdon, Ji-Chun Yang, Nathan R Zaccai, Luther J Davis, Lena Wartosch, Emily K Herman, Nicholas A Bright, James S Swarbrick, Brett M Collins, Lauren P Jackson, Matthew NJ Seaman, J Paul Luzio, Joel B Dacks, David Neuhaus, David J Owen
Sep 14, 2020
Arf6 promotes autophagosome formation via effects on phosphatidylinositol 4,5-bisphosphate and phospholipase D.
ADP-Ribosylation Factors Animals Autophagy Autophagy-Related Proteins Blotting Western CHO Cells Carrier Proteins Cell Membrane Cricetinae Endocytosis Endoplasmic Reticulum Fluorescent Antibody Technique GTPase-Activating Proteins Golgi Apparatus Green Fluorescent Proteins HeLa Cells Humans Immunosuppressive Agents Phagosomes Phosphatidylinositol 4 5-Diphosphate Phospholipase D Sirolimus TOR Serine-Threonine Kinases
RhoGAP19D inhibits Cdc42 laterally to control epithelial cell shape and prevent invasion.
Weronika Fic, Rebecca Bastock, Francesco Raimondi, Erinn Los, Yoshiko Inoue, Jennifer L Gallop, Robert B Russell, Daniel St Johnston
Jan 15, 2021
Cdc42-GTP is required for apical domain formation in epithelial cells, where it recruits and activates the Par-6-aPKC polarity complex, but how the activity of Cdc42 itself is restricted apically is unclear. We used sequence...
GTPase-activating protein Rasal1 associates with ZAP-70 of the TCR and negatively regulates T-cell tumor immunity.
Immunotherapy involving checkpoint blockades of inhibitory co-receptors is effective in combating cancer. Despite this, the full range of mediators that inhibit T-cell activation and influence anti-tumor immunity is unclear....
Animals CD4-Positive T-Lymphocytes Extracellular Signal-Regulated MAP Kinases Female GTPase-Activating Proteins Lymphocyte Activation Male Melanoma Experimental Mice Inbred BALB C Mice Inbred C57BL Mice Transgenic Protein Domains RNA Small Interfering Receptors Antigen T-Cell T-Lymphocytes ZAP-70 Protein-Tyrosine Kinase
Physical activity attenuates postprandial hyperglycaemia in homozygous TBC1D4 loss-of-function mutation carriers.
Theresia M Schnurr, Emil Jørsboe, Alexandra Chadt, Inger K Dahl-Petersen, Jonas M Kristensen, Jørgen FP Wojtaszewski, Christian Springer, Peter Bjerregaard, Søren Brage, Oluf Pedersen, Ida Moltke, Niels Grarup, Hadi Al-Hasani, Anders Albrechtsen, Marit E Jørgensen, Torben Hansen
May 03, 2021
AIMS/HYPOTHESIS: The common muscle-specific TBC1D4 p.Arg684Ter loss-of-function variant defines a subtype of non-autoimmune diabetes in Arctic populations. Homozygous carriers are characterised by elevated postprandial glucose...
Arctic Gene-Environment Interaction Lifestyle therapy physical activity Postprandial hyperglycaemia TBC1D4 loss-of-function Adult Blood Glucose Diabetes mellitus Type 2 Exercise Female GTPase-Activating Proteins Gene-Environment Interaction Genetic Predisposition to Disease Genotyping Techniques Glucose Tolerance Test Greenland Humans Hyperglycemia Insulin Inuit Life Style Loss of Function Mutation Male Middle Aged Postprandial Period
Structure of the DOCK2-ELMO1 complex provides insights into regulation of the auto-inhibited state.
Leifu Chang, Jing Yang, Chang Hwa Jo, Andreas Boland, Ziguo Zhang, Stephen H McLaughlin, Afnan Abu-Thuraia, Ryan C Killoran, Matthew J Smith, Jean-Francois Côté, David Barford
Jul 28, 2021
The WDR11 complex facilitates the tethering of AP-1-derived vesicles.
Paloma Navarro Negredo, James R Edgar, Paul T Manna, Robin Antrobus, Margaret S Robinson
Jul 20, 2018
Vesicluar transport of proteins from endosomes to the trans-Golgi network (TGN) is an essential cellular pathway, but much of its machinery is still unknown. A screen for genes involved in endosome-to-TGN trafficking produced...
Adaptor Protein Complex 1 Autoantigens CRISPR-Cas Systems Cytoplasmic Vesicles Endosomes GTPase-Activating Proteins HEK293 Cells HeLa Cells Humans Membrane Proteins Microscopy Fluorescence Multiprotein Complexes Protein Binding Protein Transport Proto-Oncogene Proteins RNA Interference trans-Golgi Network
Sar1 GTPase Activity Is Regulated by Membrane Curvature.
Michael G Hanna, Ioanna Mela, Lei Wang, Robert M Henderson, Edwin R Chapman, J Michael Edwardson, Anjon Audhya
Dec 08, 2015
COPII GTPase endoplasmic reticulum (ER) membrane bilayer membrane transport Amino Acid Substitution Animals COP-Coated Vesicles Caenorhabditis elegans Caenorhabditis elegans Proteins Endoplasmic Reticulum Enzyme Inhibitors GTP Phosphohydrolases GTPase-Activating Proteins Guanosine Triphosphate Guanylyl Imidodiphosphate Humans Lipid Bilayers Membrane Microdomains Microscopy Atomic Force Models Biological Monomeric GTP-Binding Proteins Mutation Organelle Shape RNA Interference Receptor Protein-Tyrosine Kinases Recombinant Proteins Vesicular Transport Proteins ras GTPase-Activating Proteins
Structural basis for the binding of tryptophan-based motifs by δ-COP.
Richard J Suckling, Pak Phi Poon, Sophie M Travis, Irina V Majoul, Frederick M Hughson, Philip R Evans, Rainer Duden, David J Owen
Oct 22, 2015
COPI coatomer membrane trafficking vesicle coat δ-COP μ homology domain-binding motifs Amino Acid Motifs COP-Coated Vesicles Calorimetry Indirect Cathepsin A Coatomer Protein DNA-Binding Proteins GTPase-Activating Proteins Protein Binding Protein Structure Tertiary Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Tryptophan
Thermodynamic mapping of effector protein interfaces with RalA and RalB.
Louise J Campbell, Maria Peppa, Michael D Crabtree, Arooj Shafiq, Nicholas F McGough, Helen R Mott, Darerca Owen
May 27, 2016
Arf6 Can Trigger Wave Regulatory Complex-Dependent Actin Assembly Independent of Arno.
The small GTPase ADP-ribosylation factor 6 (Arf6) anchors at the plasma membrane to orchestrate key functions, such as membrane trafficking and regulating cortical actin cytoskeleton rearrangement. A number of studies have...
Regulation of cargo-selective endocytosis by dynamin 2 GTPase-activating protein girdin.
Liang Weng, Atsushi Enomoto, Hiroshi Miyoshi, Kiyofumi Takahashi, Naoya Asai, Nobuhiro Morone, Ping Jiang, Jian An, Takuya Kato, Keisuke Kuroda, Takashi Watanabe, Masato Asai, Maki Ishida-Takagishi, Yoshiki Murakumo, Hideki Nakashima, Kozo Kaibuchi, Masahide Takahashi
May 28, 2019
In clathrin-mediated endocytosis (CME), specificity and selectivity for cargoes are thought to be tightly regulated by cargo-specific adaptors for distinct cellular functions. Here, we show that the actin-binding protein girdin...
Nbeal2 interacts with Dock7, Sec16a, and Vac14.
Louisa Mayer, Maria Jasztal, Mercedes Pardo, Salvadora Aguera de Haro, Janine Collins, Tadbir K Bariana, Peter A Smethurst, Luigi Grassi, Romina Petersen, Paquita Nurden, Rémi Favier, Lu Yu, Stuart Meacham, William J Astle, Jyoti Choudhary, Wyatt W Yue, Willem H Ouwehand, Jose A Guerrero
Mar 16, 2018
Mutations in NBEAL2, the gene encoding the scaffolding protein Nbeal2, are causal of gray platelet syndrome (GPS), a rare recessive bleeding disorder characterized by platelets lacking α-granules and progressive marrow fibrosis....
Inhibition of TBC1D5 activates Rab7a and can enhance the function of the retromer cargo-selective complex.
The retromer complex is a vital component of the endosomal protein sorting machinery necessary for sorting into both the endosome-to-Golgi retrieval pathway and also the endosome-to-cell-surface recycling pathway. Retromer...
Affinity maturation of the RLIP76 Ral binding domain to inform the design of stapled peptides targeting the Ral GTPases.
Ral GTPases have been implicated as critical drivers of cell growth and metastasis in numerous Ras-driven cancers. We have previously reported stapled peptides, based on the Ral effector RLIP76, that can disrupt Ral signaling....
GTPase K-Ras RLIP76 Ral Cancer Cell Signaling chemical biology Drug Discovery Protein-Protein Interaction stapled peptides ATP-Binding Cassette Transporters Calorimetry Circular dichroism Fluorescence GTPase-Activating Proteins Humans Magnetic Resonance Spectroscopy Protein Binding Signal Transduction ral GTP-Binding Proteins
Rho-GTPase activating-protein 18
Mohammed A Aleskandarany, Sultan Sonbul, Rachel Surridge, Abhik Mukherjee, Carlos Caldas, Maria Diez-Rodriguez, Ibraheem Ashankyty, Khalil I Albrahim, Ahmed M Elmouna, Ritu Aneja, Stewart G Martin, Ian O Ellis, Andrew R Green, Emad A Rakha
Apr 29, 2019
BACKGROUND: The prognostic value of lymphovascular invasion (LVI) in breast cancer (BC) has been demonstrated in several independent studies. However, identification of driver molecules for LVI remains a challenging task....
Multiple sclerosis genomic map implicates peripheral immune cells and microglia in susceptibility.
International Multiple Sclerosis Genetics Consortium
Aug 13, 2019
Case-Control Studies Cell Cycle Proteins Chromosome Mapping Chromosomes Human X GTPase-Activating Proteins Gene Frequency Genetic Loci Genome-Wide Association Study Genomics Humans Inheritance Patterns Major Histocompatibility Complex Microglia Multiple Sclerosis Polymorphism Single Nucleotide Quantitative Trait Loci RNA-seq Transcriptome