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Glyoxal fixation facilitates transcriptome analysis after antigen staining and cell sorting by flow cytometry.
Prasanna Channathodiyil
,
Jonathan Houseley
Mar 30, 2021
A simple method for extraction of high quality RNA from cells that have been fixed, stained and sorted by flow cytometry would allow routine transcriptome analysis of highly purified cell populations and single cells. However...
Humans
Glyoxal
Antigens
Fixatives
Flow Cytometry
Staining and Labeling
Gene Expression Profiling
MCF-7 Cells
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