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Results: 22
A Microfluidic Co-Flow Route for Human Serum Albumin-Drug-Nanoparticle Assembly.
Tuuli A Hakala, Sarah Davies, Zenon Toprakcioglu, Barbara Bernardim, Gonçalo JL Bernardes, Tuomas PJ Knowles
Apr 02, 2020
Nanoparticles are widely studied as carrier vehicles in biological systems because their size readily allows access through cellular membranes. Moreover, they have the potential to carry cargo molecules and as such, these...
High-throughput detection of ethanol-producing cyanobacteria in a microdroplet platform.
Direct Optofluidic Measurement of the Lipid Permeability of Fluoroquinolones.
Jehangir Cama, Michael Schaich, Kareem Al Nahas, Silvia Hernández-Ainsa, Stefano Pagliara, Ulrich F Keyser
Sep 30, 2016
Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells.
Burak Okumus, Dirk Landgraf, Ghee Chuan Lai, Somenath Bakshi, Juan Carlos Arias-Castro, Sadik Yildiz, Dann Huh, Raul Fernandez-Lopez, Celeste N Peterson, Erdal Toprak, Meriem El Karoui, Johan Paulsson
Jan 31, 2020
Many key regulatory proteins in bacteria are present in too low numbers to be detected with conventional methods, which poses a particular challenge for single-cell analyses because such proteins can contribute greatly to...
Ultrahigh-Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay.
Miriam Klaus, Paul Jannis Zurek, Tomasz S Kaminski, Ahir Pushpanath, Katharina Neufeld, Florian Hollfelder
Oct 13, 2021
Label-Free Analysis and Sorting of Microalgae and Cyanobacteria in Microdroplets by Intrinsic Chlorophyll Fluorescence for the Identification of Fast Growing Strains.
Roshni J Best, Jan J Lyczakowski, Sara Abalde-Cela, Ziyi Yu, Chris Abell, Alison G Smith
Nov 18, 2016
Droplet-based microfluidic analysis and screening of single plant cells.
Ziyi Yu, Christian R Boehm, Julian M Hibberd, Chris Abell, Jim Haseloff, Steven J Burgess, Ivan Reyna-Llorens
Aug 16, 2018
Agrobacterium tumefaciens Bacterial Proteins Cell Separation Drug Compounding Equipment Design Gene Expression Regulation Plant Genes Reporter Genomics High-Throughput Screening Assays Hot Temperature Lab-On-A-Chip Devices Luminescent Proteins Marchantia Microfluidic Analytical Techniques Microscopy Fluorescence Plants Genetically Modified Promoter Regions Genetic Protoplasts Single-Cell Analysis Stochastic Processes Transformation Genetic
Cooperative Assembly of Hsp70 Subdomain Clusters.
Maya A Wright, Francesco A Aprile, Mathias MJ Bellaiche, Thomas CT Michaels, Thomas Müller, Paolo Arosio, Michele Vendruscolo, Christopher M Dobson, Tuomas PJ Knowles
Sep 27, 2018
Dynamic monitoring of single cell lysis in an impedance-based microfluidic device.
Impedance spectroscopy Microfluidics Real-time measurements Single cell trapping Stem Cells Animals Cell Membrane Dimethylpolysiloxanes Electric Capacitance Electric Impedance Embryonic Stem Cells Equipment Design Evaluation Studies as Topic Lab-On-A-Chip Devices Mice Microfluidic Analytical Techniques Models Theoretical Single-Cell Analysis
Real-Time Intrinsic Fluorescence Visualization and Sizing of Proteins and Protein Complexes in Microfluidic Devices.
Pavan Kumar Challa, Quentin Peter, Maya A Wright, Yuewen Zhang, Kadi L Saar, Jacqueline A Carozza, Justin LP Benesch, Tuomas PJ Knowles
Aug 18, 2020
Optical detection has become a convenient and scalable approach to read out information from microfluidic systems. For the study of many key biomolecules, however, including peptides and proteins, which have low fluorescence...
Graphene Liquid Enclosure for Single-Molecule Analysis of Membrane Proteins in Whole Cells Using Electron Microscopy.
Indra N Dahmke, Andreas Verch, Justus Hermannsdörfer, Diana B Peckys, Robert S Weatherup, Stephan Hofmann, Niels de Jonge
May 04, 2018
Membrane proteins govern many important functions in cells via dynamic oligomerization into active complexes. However, analytical methods to study their distribution and functional state in relation to the cellular structure are...
STEM breast cancer cell epidermal growth factor receptor graphene liquid-phase electron microscopy single-molecule analysis tunneling nanotube Cell Line Tumor Graphite Humans Lab-On-A-Chip Devices Membrane Proteins Microscopy Electron Neoplasm Proteins Quantum Dots Receptor ErbB-2 Silicon Compounds single molecule imaging
Single cell studies of mouse embryonic stem cell (mESC) differentiation by electrical impedance measurements in a microfluidic device.
A microfluidic device for characterizing nuclear deformations.
Cell nuclei experience and respond to a wide range of forces, both in vivo and in vitro. In order to characterize the nuclear response to physical stress, we developed a microfluidic chip and used it to apply mechanical stress...
On-chip label-free protein analysis with downstream electrodes for direct removal of electrolysis products.
Kadi L Saar, Yingbo Zhang, Thomas Müller, Challa P Kumar, Sean Devenish, Andrew Lynn, Urszula Łapińska, Xiaoting Yang, Sara Linse, Tuomas PJ Knowles
Apr 16, 2018
The ability to apply highly controlled electric fields within microfluidic devices is valuable as a basis for preparative and analytical processes. A challenge encountered in the context of such approaches in conductive media...
Triple-Indicator-Based Multidimensional Colorimetric Sensing Platform for Heavy Metal Ion Detections.
Heavy metals are highly toxic at trace levels and their pollution has shown great threat to the environment and public health worldwide where current detection methods require expensive instrumentation and laborious operation...
color calibration Heavy metals multidimensional sensing paper-based microfluidics triple indicator Calibration Color Colorimetry Drinking Water Indicators and Reagents Lab-On-A-Chip Devices Metals Heavy Microfluidic Analytical Techniques Paper ponds Proof of Concept Study Wastewater Water Pollutants Chemical
An "occlusive thrombosis-on-a-chip" microfluidic device for investigating the effect of anti-thrombotic drugs.
Jess Berry, François J Peaudecerf, Nicole A Masters, Keith B Neeves, Raymond E Goldstein, Matthew T Harper
Aug 10, 2021
Light-induced self-assembly of active rectification devices.
Self-propelled colloidal objects, such as motile bacteria or synthetic microswimmers, have microscopically irreversible individual dynamics-a feature they share with all living systems. The incoherent behavior of individual...