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Genetic Variation in Complex Traits in Transgenic α-Synuclein Strains of Caenorhabditis elegans.
Different genetic backgrounds can modify the effect of mutated genes. Human α-synuclein (SNCA) gene encodes α-synuclein, and its oligomeric complexes accumulate with age and mediate the disruption of cellular homeostasis...
C. elegans Genetic background protein accumulation α-synuclein Animals Animals Genetically Modified Bacterial Proteins Body Size Caenorhabditis elegans Disease Models Animal Gene Expression Regulation genetic variation Genome Humans Luminescent Proteins Lysosomes Multifactorial Inheritance Parkinson Disease Transcription Genetic ALPHA-SYNUCLEIN
Structural and Functional Determinants of AC8 Trafficking, Targeting and Responsiveness in Lipid Raft Microdomains.
The fidelity of cAMP in controlling numerous cellular functions rests crucially on the precise organization of cAMP microdomains that are sustained by the scaffolding properties of adenylyl cyclase. Earlier studies suggested...
Adenylyl cyclase Caveolin Cholesterol Lipid rafts plasma membrane cAMP Adenylyl Cyclases Bacterial Proteins Brefeldin A Bridged Bicyclo Compounds Heterocyclic Caveolin 1 Cyclic AMP Cytoskeleton Gene Expression Genes Reporter Green Fluorescent Proteins Guanine Nucleotide Exchange Factors HEK293 Cells Humans Luminescent Proteins Membrane Microdomains Mutagenesis Site-Directed Protein Transport RNA Small Interfering Recombinant Fusion Proteins Thiazolidines beta-Cyclodextrins
Polarization and orientation of retinal ganglion cells in vivo.
Flavio R Zolessi, Lucia Poggi, Christopher J Wilkinson, Chi-Bin Chien, William A Harris
Jun 16, 2011
Animals Animals Genetically Modified Axons Basement Membrane Cell Polarity Cells Cultured Embryo Nonmammalian Gene Expression Regulation Developmental Imaging Three-Dimensional Luminescent Proteins Microscopy Electron Transmission Morpholines Neuroepithelial Cells Retina Retinal Ganglion Cells Time Factors Zebrafish Zebrafish Proteins
Characterisation of Drosophila Ubx CPTI000601 and hth CPTI000378 protein trap lines.
Animals Bacterial Proteins Drosophila Proteins Drosophila melanogaster Embryo Nonmammalian Founder Effect Gene Deletion Gene Expression Regulation Developmental Genes Reporter Genetic Engineering Genotype Homeodomain Proteins Homozygote Imaginal Discs Intestinal Mucosa Intestines Luminescent Proteins Phenotype Recombinant Fusion Proteins Transcription Factors
Characterisation of Drosophila Ubx CPTI000601 and hth CPTI000378 protein trap lines.
Intestines Intestinal Mucosa Embryo Nonmammalian Animals Drosophila melanogaster Bacterial Proteins Homeodomain Proteins Drosophila Proteins Luminescent Proteins Recombinant Fusion Proteins Transcription Factors Genetic Engineering Gene Expression Regulation Developmental Gene Deletion Founder Effect Genotype Homozygote Phenotype Genes Reporter Imaginal Discs
Published by: TheScientificWorldJournal
Positive Effects of NPY1 Receptor Activation on Islet Structure Are Driven by Pancreatic Alpha- and Beta-Cell Transdifferentiation in Diabetic Mice.
Ryan A Lafferty, Neil Tanday, R Charlotte Moffett, Frank Reimann, Fiona M Gribble, Peter R Flatt, Nigel Irwin
Jan 29, 2021
Enzymatically stable and specific neuropeptide Y1 receptor (NPYR1) agonists, such as sea lamprey PYY(1-36) (SL-PYY(1-36)), are believed to improve glucose regulation in diabetes by targeting pancreatic islets. In this study...
Diabetes Peptide YY sea lamprey PYY streptozotocin transdifferentiation Animals Bacterial Proteins Blood Glucose Cell Differentiation Diabetes mellitus Experimental Glucagon Glucagon-Secreting Cells Insulin Insulin-Secreting Cells Islets of Langerhans Luminescent Proteins Mice Mice Inbred C57BL Neuropeptide Y Peptides Petromyzon Streptozocin Transgenes
Optimising homing endonuclease gene drive performance in a semi-refractory species
Homing endonuclease gene (HEG) drive is a promising insect population control technique that employs meganucleases to impair the fitness of pest populations. Our previous studies showed that HEG drive was more difficult to...
Characterization of Intrinsic Properties of Promoters.
Timothy J Rudge, James R Brown, Fernan Federici, Neil Dalchau, Andrew Phillips, James W Ajioka, Jim Haseloff
Dec 08, 2015
Effects of long-acting GIP, xenin and oxyntomodulin peptide analogues on alpha-cell transdifferentiation in insulin-deficient diabetic GluCreERT2;ROSA26-eYFP mice.
Dipak Sarnobat, R Charlotte Moffett, Victor A Gault, Neil Tanday, Frank Reimann, Fiona M Gribble, Peter R Flatt, Nigel Irwin
Nov 18, 2019
Alpha-cell Glu (CreERT2) ROSA26-eYFP mice Glucose-dependent insulinotropic polypeptide (GIP) Oxytomodulin (Oxm) transdifferentiation Xenin Animals Bacterial Proteins Cell Transdifferentiation Diabetes mellitus Experimental Diabetes mellitus Type 1 Gastric Inhibitory Polypeptide Gastrointestinal Agents Glucagon-Secreting Cells Islets of Langerhans Luminescent Proteins Mice Mice Inbred C57BL Mice Transgenic Neurotensin Oxyntomodulin
Expansion microscopy of C. elegans.
Chih-Chieh Jay Yu, Nicholas C Barry, Asmamaw T Wassie, Anubhav Sinha, Abhishek Bhattacharya, Shoh Asano, Chi Zhang, Fei Chen, Oliver Hobert, Miriam B Goodman, Gal Haspel, Edward S Boyden
May 01, 2020
C. elegans developmental biology expansion microscopy Gene Expression Immunohistochemistry In Situ Hybridization neuroscience Super-resolution imaging Synapses Animals Animals Genetically Modified Caenorhabditis elegans Caenorhabditis elegans Proteins connexins DNA Fluorescent Antibody Technique Luminescent Proteins Microscopy Fluorescence Nanotechnology Neurons RNA Synapses Tissue Fixation
Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata.
Marie L Brault, Jules D Petit, Françoise Immel, William J Nicolas, Marie Glavier, Lysiane Brocard, Amèlia Gaston, Mathieu Fouché, Timothy J Hawkins, Jean-Marc Crowet, Magali S Grison, Véronique Germain, Marion Rocher, Max Kraner, Vikram Alva, Stéphane Claverol, Andrea Paterlini, Ykä Helariutta, Magali Deleu, Laurence Lins, Jens Tilsner, Emmanuelle M Bayer
Aug 22, 2019
In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved a unique type of MCS, inside intercellular pores, the plasmodesmata, where endoplasmic reticulum (ER)-plasma membrane...
ER-PM membrane contact sites intercellular communication in plants multiple C2 domains and transmembrane region proteins Plasmodesmata Arabidopsis Arabidopsis Proteins Cell Membrane Cells Cultured Endoplasmic Reticulum Gene Expression Profiling Gene Expression Regulation Developmental Gene Expression Regulation Plant Genes Reporter Glycosyltransferases Green Fluorescent Proteins Humans Luminescent Proteins Membrane Proteins Phospholipids Plant Cells Plants Genetically Modified Plasmodesmata Protein Domains Saccharomyces cerevisiae Signal Transduction Tobacco
A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling.
BACKGROUND: Intrabodies enable targeting of proteins in live cells, but generating specific intrabodies against the thousands of proteins in a proteome poses a challenge. We leverage the widespread availability of fluorescently...
cell signalling Endoplasmic Reticulum Fluorescence microscopy Fluorescent protein GFP intrabody membrane contact site Mitochondria Nanobody RFP Animals COS Cells Chlorocebus aethiops Green Fluorescent Proteins HeLa Cells Humans Luminescent Proteins Plasmids Signal Transduction Single-Domain Antibodies
Maximizing the biochemical resolving power of fluorescence microscopy.
Most recent advances in fluorescence microscopy have focused on achieving spatial resolutions below the diffraction limit. However, the inherent capability of fluorescence microscopy to non-invasively resolve different...
Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system.
Jennifer K Heppert, Daniel J Dickinson, Ariel M Pani, Christopher D Higgins, Annette Steward, Julie Ahringer, Jeffrey R Kuhn, Bob Goldstein
Oct 10, 2018
Fluorescent protein tags are fundamental tools used to visualize gene products and analyze their dynamics in vivo. Recent advances in genome editing have expedited the precise insertion of fluorescent protein tags into the...
Optogenetic evidence for inhibitory signaling from orexin to MCH neurons via local microcircuits.
John Apergis-Schoute, Panagiota Iordanidou, Cedric Faure, Sonia Jego, Cornelia Schöne, Teemu Aitta-Aho, Antoine Adamantidis, Denis Burdakov
May 11, 2015
GABA hypocretin Hypothalamus melanin-concentrating hormone optogenetics orexin Animals Calcium Channelrhodopsins Excitatory Amino Acid Antagonists Hypothalamic Area Lateral Hypothalamic Hormones In Vitro Techniques Intracellular Signaling Peptides and Proteins Luminescent Proteins Lysine Melanins Membrane Potentials Mice Nerve Net Neural Inhibition Neurons Neuropeptides Optogenetics Orexins Patch-Clamp Techniques Pituitary Hormones Signal Transduction Transduction Genetic gamma-Aminobutyric Acid
KIF1A mediates axonal transport of BACE1 and identification of independently moving cargoes in living SCG neurons.
Neurons rely heavily on axonal transport to deliver materials from the sites of synthesis to the axon terminals over distances that can be many centimetres long. KIF1A is the neuron-specific kinesin with the fastest reported...
BACE1 KIF1A Axonal Transport live-cell imaging Amyloid Precursor Protein Secretases Animals Aspartic Acid Endopeptidases Axonal Transport Cells Cultured Green Fluorescent Proteins Kinesins Luminescent Proteins Mice Inbred C57BL Microscopy Fluorescence Motor Neurons Nicotinamide-Nucleotide Adenylyltransferase Protein Transport Signal Transduction Superior Cervical Ganglion
RNA Docking and Local Translation Regulate Site-Specific Axon Remodeling In Vivo
CE Holt, H-W Wong, Q Lin, F Stroehl, CG Roque, JM Cioni, R Cagnetta, B Turner-Bridger, R Laine, WA Harris, CF Kaminski
Jul 28, 2017
FRAP RNA labeling RNA localization RNA trafficking axon branching Axon guidance local protein synthesis Mitochondria neural wiring Retinotectal projection β-actin Actins Animals Anisomycin Axons Biotin Blastomeres Carbocyanines Cycloheximide Deoxyuracil Nucleotides Embryo Nonmammalian Gene Expression Regulation Developmental In Vitro Techniques Luminescent Proteins Mitochondria Morpholinos Oligonucleotides Antisense Organ Culture Techniques Protein Synthesis Inhibitors RNA Retina Xenopus laevis
Defining multiplicity of vector uptake in transfected Plasmodium parasites.
Manuela Carrasquilla, Sophie Adjalley, Theo Sanderson, Alejandro Marin-Menendez, Rachael Coyle, Ruddy Montandon, Julian C Rayner, Alena Pance, Marcus CS Lee
Sep 09, 2020
The recurrent emergence of drug resistance in Plasmodium falciparum increases the urgency to genetically validate drug resistance mechanisms and identify new targets. Reverse genetics have facilitated genome-scale knockout...
Small-RNA Sequencing Reveals Altered Skeletal Muscle microRNAs and snoRNAs Signatures in Weanling Male Offspring from Mouse Dams Fed a Low Protein Diet during Lactation.
Ioannis Kanakis, Moussira Alameddine, Leighton Folkes, Simon Moxon, Ioanna Myrtziou, Susan E Ozanne, Mandy J Peffers, Katarzyna Goljanek-Whysall, Aphrodite Vasilaki
May 20, 2021
Maternal diet during gestation and lactation affects the development of skeletal muscles in offspring and determines muscle health in later life. In this paper, we describe the association between maternal low protein...
maternal protein restriction MicroRNAs Muscle Development Offspring sncRNAs snoRNAs Animals Bacterial Proteins Computational Biology Diet Protein-Restricted Female Lactation Luminescent Proteins Male Maternal Nutritional Physiological Phenomena Mice MicroRNAs Muscle Development Muscle Skeletal RNA Small Nucleolar RNA Small Untranslated Sequence Analysis DNA Weaning
Two-colour live-cell nanoscale imaging of intracellular targets.
Francesca Bottanelli, Emil B Kromann, Edward S Allgeyer, Roman S Erdmann, Stephanie Wood Baguley, George Sirinakis, Alanna Schepartz, David Baddeley, Derek K Toomre, James E Rothman, Joerg Bewersdorf
Jun 07, 2021
Stimulated emission depletion (STED) nanoscopy allows observations of subcellular dynamics at the nanoscale. Applications have, however, been severely limited by the lack of a versatile STED-compatible two-colour labelling...
Droplet-based microfluidic analysis and screening of single plant cells.
Ziyi Yu, Christian R Boehm, Julian M Hibberd, Chris Abell, Jim Haseloff, Steven J Burgess, Ivan Reyna-Llorens
Aug 16, 2018
Agrobacterium tumefaciens Bacterial Proteins Cell Separation Drug Compounding Equipment Design Gene Expression Regulation Plant Genes Reporter Genomics High-Throughput Screening Assays Hot Temperature Lab-On-A-Chip Devices Luminescent Proteins Marchantia Microfluidic Analytical Techniques Microscopy Fluorescence Plants Genetically Modified Promoter Regions Genetic Protoplasts Single-Cell Analysis Stochastic Processes Transformation Genetic
Subcellular localisations of the CPTI collection of YFP-tagged proteins in Drosophila embryos.
Epithelium GFP Morphogenesis Protein trap Animals Bacterial Proteins Cell Nucleus Centrosome Cytokinesis Cytoplasm Drosophila Proteins Drosophila melanogaster Gene Expression Profiling Gene Expression Regulation Developmental Genetic Techniques Green Fluorescent Proteins Luminescent Proteins Mitochondria
Lateral adhesion drives reintegration of misplaced cells into epithelial monolayers.
Adherens Junctions Animals Animals Genetically Modified Cell Adhesion Cell Adhesion Molecules Neuronal Cell Cycle Proteins Cell Division Cytoskeletal Proteins Drosophila Proteins Drosophila melanogaster Embryo Nonmammalian Epithelial Cells Epithelium Female Guanine Nucleotide Dissociation Inhibitors Luminescent Proteins Membrane Proteins Microscopy Fluorescence Mutation Nerve Tissue Proteins Ovary RNA Interference Video Recording
Tracing the cellular basis of islet specification in mouse pancreas.
Magdalena K Sznurkowska, Edouard Hannezo, Roberta Azzarelli, Lemonia Chatzeli, Tatsuro Ikeda, Shosei Yoshida, Anna Philpott, Benjamin D Simons
Sep 16, 2020
Animals Cell Differentiation Cell Lineage Computer Simulation Embryo Mammalian Embryonic Development Female Genes Reporter Glucagon-Secreting Cells Imaging Three-Dimensional Insulin-Secreting Cells Luminescent Proteins Male Mice Mice Transgenic Microscopy Confocal Models Animal Models Biological Organogenesis Pancreas Stem Cells
Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library.
Nick Lowe, Johanna S Rees, John Roote, Ed Ryder, Irina M Armean, Glynnis Johnson, Emma Drummond, Helen Spriggs, Jenny Drummond, Jose P Magbanua, Huw Naylor, Bénédicte Sanson, Rebecca Bastock, Sven Huelsmann, Vitor Trovisco, Matthias Landgraf, Seymour Knowles-Barley, J Douglas Armstrong, Helen White-Cooper, Celia Hansen, Roger G Phillips, UK Drosophila Protein Trap Screening Consortium, Kathryn S Lilley, Steven Russell, Daniel St Johnston
Jan 18, 2016
Affinity purification Cytoophidia Live imaging Protein trap piggyBac Animals Bacterial Proteins Crosses Genetic Drosophila Proteins Drosophila melanogaster Exons Female Gene Expression Profiling Gene Expression Regulation Developmental Genetic Techniques Genome Luminescent Proteins Male Membrane Proteins Ovary Sex Factors Testis Transcription Genetic
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