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Intrinsic challenges in ancient microbiome reconstruction using 16S rRNA gene amplification.
Kirsten A Ziesemer, Allison E Mann, Krithivasan Sankaranarayanan, Hannes Schroeder, Andrew T Ozga, Bernd W Brandt, Egija Zaura, Andrea Waters-Rist, Menno Hoogland, Domingo C Salazar-García, Mark Aldenderfer, Camilla Speller, Jessica Hendy, Darlene A Weston, Sandy J MacDonald, Gavin H Thomas, Matthew J Collins, Cecil M Lewis, Corinne Hofman, Christina Warinner
Nov 22, 2018
To date, characterization of ancient oral (dental calculus) and gut (coprolite) microbiota has been primarily accomplished through a metataxonomic approach involving targeted amplification of one or more variable regions in the...