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Ninein is essential for apico-basal microtubule formation and CLIP-170 facilitates its redeployment to non-centrosomal microtubule organizing centres.
Deborah A Goldspink, Chris Rookyard, Benjamin J Tyrrell, Jonathan Gadsby, James Perkins, Elizabeth K Lund, Niels Galjart, Paul Thomas, Tom Wileman, Mette M Mogensen
Oct 10, 2018
Differentiation of columnar epithelial cells involves a dramatic reorganization of the microtubules (MTs) and centrosomal components into an apico-basal array no longer anchored at the centrosome. Instead, the minus-ends of the...
CLIP-170 IQGAP1 Rac1 Microtubules ninein non-centrosomal MTOCs Animals Cell Differentiation Cell Line Cell Polarity Cell Shape Cytoskeletal Proteins Dogs Epithelial Cells Gene Knockout Techniques Humans Madin Darby Canine Kidney Cells Mice Microtubule-Associated Proteins Microtubule-Organizing Center Microtubules Neoplasm Proteins
Mechanical design principles of a mitotic spindle.
An organised spindle is crucial to the fidelity of chromosome segregation, but the relationship between spindle structure and function is not well understood in any cell type. The anaphase B spindle in fission yeast has a...
Molecular subversion of Cdc42 signalling in cancer.
Trypanosome KKIP1 Dynamically Links the Inner Kinetochore to a Kinetoplastid Outer Kinetochore Complex.
Kinetochores perform an essential role in eukaryotes, coupling chromosomes to the mitotic spindle. In model organisms they are composed of a centromere-proximal inner kinetochore and an outer kinetochore network that binds to...
Force- and length-dependent catastrophe activities explain interphase microtubule organization in fission yeast.
The cytoskeleton is essential for the maintenance of cell morphology in eukaryotes. In fission yeast, for example, polarized growth sites are organized by actin, whereas microtubules (MTs) acting upstream control where growth...
Computer simulations reveal motor properties generating stable antiparallel microtubule interactions.
François Nédélec
Jul 30, 2020
An aster of microtubules is a set of flexible polar filaments with dynamic plus ends that irradiate from a common location at which the minus ends of the filaments are found. Processive soluble oligomeric motor complexes can...
Subcellular and supracellular mechanical stress prescribes cytoskeleton behavior in Arabidopsis cotyledon pavement cells.
Arun Sampathkumar, Pawel Krupinski, Raymond Wightman, Pascale Milani, Alexandre Berquand, Arezki Boudaoud, Olivier Hamant, Henrik Jönsson, Elliot M Meyerowitz
Apr 16, 2020
Although it is a central question in biology, how cell shape controls intracellular dynamics largely remains an open question. Here, we show that the shape of Arabidopsis pavement cells creates a stress pattern that controls...
Self-Organization of Minimal Anaphase Spindle Midzone Bundles.
Jonathon Hannabuss, Manuel Lera-Ramirez, Nicholas I Cade, Franck J Fourniol, François Nédélec, Thomas Surrey
Jul 25, 2019
In anaphase spindles, antiparallel microtubules associate to form tight midzone bundles, as required for functional spindle architecture and correct chromosome segregation. Several proteins selectively bind to these overlaps to...
A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin.
Grégory Menchon, Andrea E Prota, Daniel Lucena-Agell, Pascal Bucher, Rolf Jansen, Herbert Irschik, Rolf Müller, Ian Paterson, J Fernando Díaz, Karl-Heinz Altmann, Michel O Steinmetz
Aug 20, 2018
Microtubule-targeting agents (MTAs) like taxol and vinblastine are among the most successful chemotherapeutic drugs against cancer. Here, we describe a fluorescence anisotropy-based assay that specifically probes for ligands...
Localised dynactin protects growing microtubules to deliver oskar mRNA to the posterior cortex of the Drosophila oocyte.
Ross Nieuwburg, Dmitry Nashchekin, Maximilian Jakobs, Andrew P Carter, Philipp Khuc Trong, Raymond Goldstein, Robert St Johnston
May 03, 2018
© Nieuwburg et al. The localisation of oskar mRNA to the posterior of the Drosophila oocyte defines where the abdomen and germ cells form in the embryo. Kinesin 1 transports oskar mRNA to the oocyte posterior along a polarised...
Oocytes Microtubules Animals Drosophila melanogaster Kinesin Cytoskeletal Proteins Drosophila Proteins RNA Messenger
Published by: eLife
Reversal of contractility as a signature of self-organization in cytoskeletal bundles.
Martin Lenz
Mar 25, 2020
WD40-repeat 47, a microtubule-associated protein, is essential for brain development and autophagy.
Meghna Kannan, Efil Bayam, Christel Wagner, Bruno Rinaldi, Perrine F Kretz, Peggy Tilly, Marna Roos, Lara McGillewie, Séverine Bär, Shilpi Minocha, Claire Chevalier, Chrystelle Po, Sanger Mouse Genetics Project, Jamel Chelly, Jean-Louis Mandel, Renato Borgatti, Amélie Piton, Craig Kinnear, Ben Loos, David J Adams, Yann Hérault, Stephan C Collins, Sylvie Friant, Juliette D Godin, Binnaz Yalcin
Feb 24, 2021
The family of WD40-repeat (WDR) proteins is one of the largest in eukaryotes, but little is known about their function in brain development. Among 26 WDR genes assessed, we found 7 displaying a major impact in neuronal...
Autoinhibition of Cnn binding to γ-TuRCs prevents ectopic microtubule nucleation and cell division defects.
Corinne A Tovey, Chisato Tsuji, Alice Egerton, Fred Bernard, Antoine Guichet, Marc de la Roche, Paul T Conduit
May 11, 2021
γ-Tubulin ring complexes (γ-TuRCs) nucleate microtubules. They are recruited to centrosomes in dividing cells via binding to N-terminal CM1 domains within γ-TuRC-tethering proteins, including Drosophila Centrosomin (Cnn)....
Animals Animals Genetically Modified Cell Cycle Proteins Cell Division Drosophila Proteins Drosophila melanogaster Female Fertility HEK293 Cells Homeodomain Proteins Humans Male Microscopy Confocal Microscopy Fluorescence Microtubule-Associated Proteins Microtubules Nerve Tissue Proteins Phosphorylation Protein Binding Protein Interaction Domains and Motifs Structure-Activity Relationship
Alpha-synuclein aggresomes inhibit ciliogenesis and multiple functions of the centrosome.
Anila Iqbal, Marta Baldrighi, Jennifer N Murdoch, Angeleen Fleming, Christopher J Wilkinson
Oct 26, 2020
Protein aggregates are the pathogenic hallmarks of many different neurodegenerative diseases and include the accumulation of α-synuclein, the main component of Lewy bodies found in Parkinson's disease. Aggresomes are...
Secondary cell wall patterning-connecting the dots, pits and helices.
Huizhen Xu, Alessandro Giannetti, Yuki Sugiyama, Wenna Zheng, René Schneider, Yoichiro Watanabe, Yoshihisa Oda, Staffan Persson
Jan 04, 2023
Chemical inhibition of NAT10 corrects defects of laminopathic cells.
Delphine Larrieu, Sébastien Britton, Mukerrem Demir, Raphaël Rodriguez, Stephen P Jackson
Aug 02, 2019
KATANIN-dependent mechanical properties of the stigmatic cell wall mediate the pollen tube path in Arabidopsis.
Lucie Riglet, Frédérique Rozier, Chie Kodera, Simone Bovio, Julien Sechet, Isabelle Fobis-Loisy, Thierry Gaude
Oct 19, 2020
Successful fertilization in angiosperms depends on the proper trajectory of pollen tubes through the pistil tissues to reach the ovules. Pollen tubes first grow within the cell wall of the papilla cells, applying pressure to the...
Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins.
Lysosome-related organelles (LROs) are endosomal compartments carrying tissue-specific proteins, which become enlarged in Chediak-Higashi syndrome (CHS) due to mutations in LYST. Here, we show that Drosophila Mauve, a...
Chediak-Higashi syndrome Drosophila LYST centrosomes endosomal vesicle trafficking lysosome-related organelles mauve microtubule nucleation minispindles/Ch-TOG Animals Cell Line Centrosome Chediak-Higashi Syndrome Cytoplasmic Granules Drosophila Drosophila Proteins Female Humans Lysosomes Microtubule-Associated Proteins Microtubules Mutation Oocytes Spindle Apparatus Vesicular Transport Proteins
Kinesin-4 Functions in Vesicular Transport on Cortical Microtubules and Regulates Cell Wall Mechanics during Cell Elongation in Plants.
Zhaosheng Kong, Motohide Ioki, Siobhan Braybrook, Shundai Li, Zheng-Hua Ye, Yuh-Ru Julie Lee, Takashi Hotta, Anny Chang, Juan Tian, Guangda Wang, Bo Liu
Jun 02, 2015
Network of protein interactions within the Drosophila inner kinetochore.
Magdalena M Richter, Jaroslaw Poznanski, Anna Zdziarska, Mariusz Czarnocki-Cieciura, Zoltan Lipinszki, Michal Dadlez, David M Glover, Marcin R Przewloka
Feb 03, 2016
HDX-MS Centromere CHROMOSOME kinetochore Mitosis structure Amino Acid Sequence Animals Centromere Chromosomal Proteins Non-Histone Drosophila Drosophila Proteins Kinetochores Microtubules Models Molecular Molecular Sequence Data Nuclear Proteins Protein Binding Protein Conformation Protein Interaction Domains and Motifs Protein Interaction Mapping Protein Interaction Maps Protein Multimerization Sequence Alignment Transcription Factors Vesicular Transport Proteins
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