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The lumen of the zebrafish neural tube develops precisely at the midline of the solid neural rod primordium. This process depends on cell polarisation and cell rearrangements, both of which are manifest at the midline of the...

Cell Division Centrosome Neural Tube Zebrafish Animals Brain Cell Polarity Neural Tube Zebrafish

Animals Cell Cycle Cell Cycle Proteins Cell Differentiation Cell Proliferation Central Nervous System Gene Expression Regulation Developmental Gene Expression Regulation Enzymologic Models Animal Neural Tube Neurons S-Phase Kinase-Associated Proteins Stem Cells Ubiquitin-Protein Ligases Xenopus Proteins Xenopus laevis

BACKGROUND: Morphogenesis of the zebrafish neural tube requires the coordinated movement of many cells in both time and space. A good example of this is the movement of the cells in the zebrafish neural plate as they converge...

Animals Body Patterning Cell Movement Mesoderm Neural Plate Neural Tube Nodal Signaling Ligands Zebrafish

Epithelial fusion is a key process of morphogenesis by which tissue connectivity is established between adjacent epithelial sheets. A striking and poorly understood feature of this process is "zippering," whereby a fusion point...

Cell Adhesion epithelial zippering Extracellular Matrix Fibronectin Fusion gap closure Integrins Morphogenesis Neurulation spina bifida Actomyosin Animals Cell Fusion Embryo Mammalian Epithelial Cells Female focal adhesions Integrin beta1 Mice Mice Inbred BALB C Mice Inbred C57BL Mice Knockout Morphogenesis Neural Crest Neural Tube Neurulation

Animals Body Patterning Immunohistochemistry Microscopy Confocal Neural Stem Cells Neural Tube Neurogenesis Zebrafish

By analysing the cellular and subcellular events that occur in the centre of the developing zebrafish neural rod, we have uncovered a novel mechanism of cell polarisation during lumen formation. Cells from each side of the...

Animals Body Patterning Carrier Proteins Cell Communication Cell Division Cell Movement Cell Polarity Embryo Nonmammalian Green Fluorescent Proteins Luminescent Agents Microtubules Mutation Neural Tube Neurulation Nocodazole Protein Transport Recombinant Fusion Proteins Tubulin Modulators Zebrafish Zebrafish Proteins rab GTP-Binding Proteins

Using the zebrafish neural tube as a model, we uncover the in vivo mechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that Mpp5a and...

Adhesions Epithelial tube Morphogenesis Neural Tube Zebrafish Animals Cell Polarity Epithelial Cells Gene Expression Regulation Developmental Guanylate Cyclase Intercellular Junctions Membrane Proteins Morphogenesis Neural Tube Zebrafish Zebrafish Proteins rab GTP-Binding Proteins

The neural tube is an important model system of morphogenesis representing the developmental module of out-of-plane epithelial deformation. As the embryonic precursor of the central nervous system, the neural tube also holds...

Mechanical forces mechanical properties Morphogenesis Neural Tube Biomechanical Phenomena Central Nervous System Models Biological Morphogenesis Neural Tube

Animals Cell Lineage Embryo Mammalian Embryonic Stem Cells Endoderm Gastrulation Heart Mesencephalon Mice Models Biological Neural Tube Neurulation Organogenesis PAX6 Transcription Factor Prosencephalon Somites