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We report a technique to selectively and continuously label the proteomes of individual cell types in coculture, named cell type-specific labeling using amino acid precursors (CTAP). Through transgenic expression of exogenous...

Animals Base Sequence Cell Line Coculture Techniques Humans Isotope Labeling Lysine Mice Molecular Sequence Data Oligonucleotide Array Sequence Analysis Organisms Genetically Modified Proteome Proteomics RNA Messenger Sequence Analysis DNA Tandem Mass Spectrometry

Rubber tree (Hevea brasiliensis Muell. Arg) is an important economic crop in Thailand. Leaf fall and black stripe diseases caused by the aggressive oomycete pathogen Phytophthora palmivora, cause deleterious damage on rubber...

Chromatography Affinity Disease Resistance Escherichia coli Gene Transfer Techniques Hevea Host-Pathogen Interactions Immunoprecipitation Organisms Genetically Modified Phytophthora Plant Proteins Polymerase Chain Reaction Protease Inhibitors Recombinant Proteins Serine Proteases Tobacco

Animals CRISPR-Cas Systems Clustered Regularly Interspaced Short Palindromic Repeats Containment of Biohazards Endonucleases Genetic Engineering Genetic Research Genome Organisms Genetically Modified Safety

TNFα is a pleiotropic pro-inflammatory cytokine with a key role in the activation of the immune system to fight viral infections. Despite its antiviral role, a few viruses might utilize the host produced TNFα to their benefit....

Animals Autophagy Blotting Western Disease Models Animal Fluorescent Antibody Technique host-parasite interactions Organisms Genetically Modified Polymerase Chain Reaction rhabdoviridae Rhabdoviridae Infections Tumor Necrosis Factor-alpha Virus Replication Zebrafish

Original Article animal consciousness Animal minds Organisms Origins of consciousness Phenomenological complexity

The fast-paced field of synthetic biology is fundamentally changing the global biosecurity framework. Current biosecurity regulations and strategies are based on previous governance paradigms for pathogen-oriented security...

Containment of Biohazards DNA Recombinant Humans Industrial Development Internationality Medicine Organisms Genetically Modified Policy Making Risk Factors Social Sciences synthetic biology Virulence

Mitochondria oligomeric state Protein complex Pyruvate transport proteins Anion Transport Proteins Gene Expression Regulation Fungal Mitochondrial Membrane Transport Proteins Monocarboxylic Acid Transporters Multiprotein Complexes Organisms Genetically Modified Protein Multimerization Protein Structure Quaternary Pyruvic Acid Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Structure-Activity Relationship Temperature

Persisters represent a small subpopulation of non- or slow-growing bacterial cells that are tolerant to killing by antibiotics. Despite their prominent role in the recalcitrance of chronic infections to antibiotic therapy, the...

Anti-Bacterial Agents Bacteria Bacterial Physiological Phenomena Citric Acid Cycle Drug Resistance Multiple Bacterial Energy Metabolism Escherichia coli Microbial Sensitivity Tests Microbial Viability Organisms Genetically Modified

Understanding the evolutionary history of organisms allows us to better comprehend selective pressures and their effects on larger populations. In our study, we focused on analyzing the DNA of ciliate groups, which are single...

Bio Informatics Computer Program DNA Translation Ciliates Codons Bioinformatics Biology mathematics Organisms Physical Sciences and Mathematics software engineering

Saccharomyces cerevisiae is one of the most popular expression systems for eukaryotic membrane proteins. Here, we describe protocols for the expression and purification of mitochondrial membrane proteins developed in our...

Membrane Proteins Mitochondria Nickel affinity chromatography Protein expression Purification by on-column proteolytic cleavage Saccharomyces cerevisiae Yeast Chromatography Affinity Chromatography Gel Cloning Molecular Escherichia coli Gene Expression Regulation Fungal Genetic Vectors Green Fluorescent Proteins Membrane Proteins Mitochondrial Membrane Transport Proteins Nickel Organisms Genetically Modified Proteolysis Recombinant Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Solubility Transformation Bacterial

Abstract: In dicephalus conjoined twinning, it appears that two heads share a body; in cephalopagus, it appears that two bodies share a head. How many human animals are present in these cases? One answer is that there are two in...

article Biological individuation Conjoined twins Animals Organisms Animalism Personal identity