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Although the invention of right heart catheterisation in the 1950s enabled accurate clinical diagnosis of pulmonary arterial hypertension (PAH), it was not until 2000 when the landmark discovery of the causative role of bone...

forward phenotyping forward genetics Reverse Genetics reverse phenotyping pulmonary arterial hypertension intermediate phenotypes whole-genome sequencing epigenetic inheritance Genetic Heterogeneity phenotypic heterogeneity

Viroplasms are cytoplasmic, membraneless structures assembled in rotavirus (RV)-infected cells, which are intricately involved in viral replication. Two virus-encoded, non-structural proteins, NSP2 and NSP5, are the main drivers...

Crispr-Csy4 Genome Editing Nsp2 Nsp5 liquid-liquid phase separation Protein-Rna Condensates Replication Cycle Reverse Genetics Rotavirus Viroplasm Animals Capsid Proteins Cytoplasm Cytosol Humans Phosphorylation RNA-Binding Proteins Rotavirus Rotavirus Infections Viral Nonstructural Proteins Viral Replication Compartments Virus Assembly Virus Replication

Viroplasms are cytoplasmic, membraneless structures assembled in rotavirus (RV)-infected cells, which are intricately involved in viral replication. Two virus-encoded, non-structural proteins, NSP2 and NSP5, are the main drivers...

Rotavirus Viroplasm Replication Cycle Nsp5 Nsp2 Reverse Genetics Crispr-Csy4 Genome Editing Protein-Rna Condensates liquid-liquid phase separation

Amino Acid Substitution Amino Acids Animals Binding Sites Cell Line Cricetinae DNA Mutational Analysis Electrophoretic Mobility Shift Assay Mutagenesis Site-Directed Norovirus Protein Binding RNA Viral RNA-Dependent RNA Polymerase Reverse Genetics Virus Replication

RESEARCH ARTICLE Plant genomics Long read sequencing genome assembly Structural variants Translocations chromosome fusions Reverse Genetics Chromosomal rearrangements GABI-Kat

Viroplasms are cytoplasmic, membraneless structures assembled in rotavirus (RV)-infected cells, which are intricately involved in viral replication. Two virus-encoded, non-structural proteins, NSP2 and NSP5, are the main drivers...

Reverse Genetics Rotavirus Replication Cycle Nsp2 liquid-liquid phase separation Viroplasm Protein-Rna Condensates Nsp5 Crispr-Csy4 Genome Editing

The skin is a highly regenerative organ which plays critical roles in protecting the body and sensing its environment. Consequently, morbidity and mortality associated with skin defects represent a significant health issue. To...

Animals Embryonic Stem Cells Hair Follicle Mice Mutation Phenotype Reverse Genetics Skin Physiological Phenomena

Caenorhabditis elegans has a complete annotated genome sequence that is augmented by increasing quantities of data from high-throughput postgenomic analyses. This has led to an increasing need to identify the biological...

Animals Biolistics Caenorhabditis elegans Gene Targeting Genes Helminth Genetic Engineering Genome Helminth Genomics Mutagenesis Recombination Genetic Reverse Genetics

1-Alkyl-2-acetylglycerophosphocholine Esterase Apolipoprotein C-III Cohort Studies Consanguinity Coronary Disease Cytochrome P450 Family 2 DNA Mutational Analysis Dietary Fats Exome Fasting Female Gene Deletion Gene Frequency Genes Genetic Association Studies Homozygote Humans Interleukin-8 Male Middle Aged Myocardial Infarction neuregulins Pakistan Pedigree Phenotype Phosphoproteins Postprandial Period RNA Splice Sites Reverse Genetics Sodium-Hydrogen Antiporter Triglycerides

The Norovirus genus contains important human pathogens, but the role of host pathways in norovirus replication is largely unknown. Murine noroviruses provide the opportunity to study norovirus replication in cell culture and in...

noroviruses plus-strand RNA virus protein structure-function reverse genetic analysis viral replication virus-host interactions Amino Acid Motifs Animals Cell Line HEK293 Cells Host-Pathogen Interactions Humans Mice Norovirus Phenylalanine RAW 264.7 Cells RNA Viral Reverse Genetics Vesicular Transport Proteins Viral Nonstructural Proteins Virus Replication

Binding Sites DNA DNA-Binding Proteins Humans Internet Machine Learning Models Molecular Models Statistical Mutation Missense Protein Binding Protein Conformation alpha-Helical Protein Conformation beta-Strand Protein Interaction Domains and Motifs Protein Stability RNA-Binding Proteins Reverse Genetics Software Thermodynamics

BACKGROUND: Experimental proof of gene function assignments in plants is based on mutant analyses. T-DNA insertion lines provided an invaluable resource of mutants and enabled systematic reverse genetics-based investigation of...

Chromosomal rearrangements chromosome fusions GABI-Kat genome assembly Long read sequencing Reverse Genetics Structural variants Translocations Arabidopsis DNA Bacterial Genomics Mutagenesis Insertional

Although the invention of right heart catheterisation in the 1950s enabled accurate clinical diagnosis of pulmonary arterial hypertension (PAH), it was not until 2000 when the landmark discovery of the causative role of bone...

epigenetic inheritance forward genetics forward phenotyping Genetic Heterogeneity intermediate phenotypes phenotypic heterogeneity pulmonary arterial hypertension Reverse Genetics reverse phenotyping whole-genome sequencing Animals Bone Morphogenetic Protein Receptors Type II Genetic Predisposition to Disease Humans Mutation Phenotype pulmonary arterial hypertension