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Assessment and Translation of the Antibody-in-Lymphocyte Supernatant (ALS) Assay to Improve the Diagnosis of Enteric Fever in Two Controlled Human Infection Models and an Endemic Area of Nepal.
Thomas C Darton, Claire Jones, Sabina Dongol, Merryn Voysey, Christoph J Blohmke, Rajendra Shrestha, Abhilasha Karkey, Mila Shakya, Amit Arjyal, Claire S Waddington, Malick Gibani, Michael J Carter, Buddha Basnyat, Stephen Baker, Andrew J Pollard
Apr 10, 2019
New diagnostic tests for enteric fever are urgently needed to assist with timely antimicrobial treatment of patients and to measure the efficacy of prevention measures such as vaccination. In a novel translational approach, here...
Evaluation of the Clinical and Microbiological Response to Salmonella Paratyphi A Infection in the First Paratyphoid Human Challenge Model.
Hazel C Dobinson, Malick M Gibani, Claire Jones, Helena B Thomaides-Brears, Merryn Voysey, Thomas C Darton, Claire S Waddington, Danielle Campbell, Iain Milligan, Liqing Zhou, Sonu Shrestha, Simon A Kerridge, Anna Peters, Zoe Stevens, Audino Podda, Laura B Martin, Flavia D'Alessio, Duy Pham Thanh, Buddha Basnyat, Stephen Baker, Brian Angus, Myron M Levine, Christoph J Blohmke, Andrew J Pollard
Apr 10, 2019
BACKGROUND: To expedite the evaluation of vaccines against paratyphoid fever, we aimed to develop the first human challenge model of Salmonella enterica serovar Paratyphi A infection. METHODS: Two groups of 20 participants...
Evaluating PCR-Based Detection of Salmonella Typhi and Paratyphi A in the Environment as an Enteric Fever Surveillance Tool.
Senjuti Saha, Arif M Tanmoy, Jason R Andrews, Mohammad SI Sajib, Alexander T Yu, Stephen Baker, Stephen P Luby, Samir K Saha
Aug 04, 2021
With prequalification of a typhoid conjugate vaccine by the World Health Organization, countries are deciding whether and at what geographic scale to provide the vaccine. Optimal local data to clarify typhoid risk are expensive...
The STRATAA study protocol
Thomas C Darton, James E Meiring, Susan Tonks, Md Arifuzzaman Khan, Farhana Khanam, Mila Shakya, Deus Thindwa, Stephen Baker, Buddha Basnyat, John D Clemens, Gordon Dougan, Christiane Dolecek, Sarah J Dunstan, Melita A Gordon, Robert S Heyderman, Kathryn E Holt, Virginia E Pitzer, Firdausi Qadri, K Zaman, Andrew J Pollard, STRATAA Study Consortium
Apr 10, 2019
INTRODUCTION: Invasive infections caused by Salmonella enterica serovar Typhi and Paratyphi A are estimated to account for 12-27 million febrile illness episodes worldwide annually. Determining the true burden of typhoidal...
Africa Asia Diagnosis enteric fever febrile illness healthcare utilisation infection transmission resource-limited setting Salmonella paratyphi A Salmonella typhi seroepidemiology serosurveillance vaccination programme Adolescent Bangladesh Carrier State Censuses Child Child Preschool Cost of Illness Female Health Resources Humans Incidence Infant Infant Newborn Malawi Male Models Theoretical Nepal Patient Acceptance of Health Care Population Surveillance Research Design Seroepidemiologic Studies Surveys and Questionnaires Typhoid Fever
Genetic diversity of Salmonella Paratyphi A isolated from enteric fever patients in Bangladesh from 2008 to 2018.
Sadia Isfat Ara Rahman, To Nguyen Thi Nguyen, Farhana Khanam, Nicholas R Thomson, Zoe A Dyson, Alyce Taylor-Brown, Emran Kabir Chowdhury, Gordon Dougan, Stephen Baker, Firdausi Qadri
Nov 12, 2021
BACKGROUND: The proportion of enteric fever cases caused by Salmonella Paratyphi A is increasing and may increase further as we begin to introduce typhoid conjugate vaccines (TCVs). While numerous epidemiological and genomic...
Very long O-antigen chains of Salmonella Paratyphi A inhibit inflammasome activation and pyroptotic cell death.
Elli Mylona, Julia Sanchez-Garrido, Trang Nguyen Hoang Thu, Sabina Dongol, Abhilasha Karkey, Stephen Baker, Avinash R Shenoy, Gad Frankel
Jan 22, 2021
Salmonella Paratyphi A (SPtA) remains one of the leading causes of enteric (typhoid) fever. Yet, despite the recent increased rate of isolation from patients in Asia, our understanding of its pathogenesis is incomplete. Here we...
FepE O-antigen Salmonella paratyphi A Salmonella typhimurium caspase-4 enteric fever Inflammasomes pyroptosis Bacterial Proteins Caspases HeLa Cells Humans Inflammasomes Intracellular Signaling Peptides and Proteins MACROPHAGES O Antigens Paratyphoid Fever Phosphate-Binding Proteins pyroptosis Salmonella paratyphi A THP-1 Cells Type III Secretion Systems Virulence Virulence Factors
Multiplex PCR assay to detect high risk lineages of Salmonella Typhi and Paratyphi A.
Fahad Khokhar, Derek Pickard, Zoe Dyson, Junaid Iqbal, Agila Pragasam, Jobin Jacob John, Balaji Veeraraghavan, Farah Qamar, Gordon Dougan, Hilary MacQueen, Sushila Rigas, Mark Holmes, Ankur Mutreja
Aug 21, 2022
Salmonella Paratyphi A Outer Membrane Vesicles Displaying Vi Polysaccharide as a Multivalent Vaccine against Enteric Fever.
G Gasperini, R Alfini, V Arato, F Mancini, MG Aruta, P Kanvatirth, D Pickard, F Necchi, A Saul, O Rossi, F Micoli, P Mastroeni
Dec 11, 2020
Treatment Response in Enteric Fever in an Era of Increasing Antimicrobial Resistance
Corinne N Thompson, Abhilasha Karkey, Sabina Dongol, Amit Arjyal, Marcel Wolbers, Thomas Darton, Jeremy J Farrar, Guy E Thwaites, Christiane Dolecek, Buddha Basnyat, Stephen Baker
Apr 10, 2019
BACKGROUND.: Enteric fever, caused by Salmonella Typhi and Salmonella Paratyphi A, is the leading cause of bacterial febrile disease in South Asia. METHODS.: Individual data from 2092 patients with enteric fever randomized into...
Nepal Antimicrobial resistance enteric fever fluoroquinolone typhoid Adolescent Adult Anti-Bacterial Agents Azithromycin Ceftriaxone Child Drug Resistance Multiple Bacterial Female Fluoroquinolones Gatifloxacin Humans Male Microbial Sensitivity Tests Nepal Ofloxacin Paratyphoid Fever Salmonella paratyphi A Salmonella typhi Treatment Failure Treatment Outcome Typhoid Fever Young Adult
Paratype
Arif M Tanmoy, Yogesh Hooda, Mohammad SI Sajib, Kesia E da Silva, Junaid Iqbal, Farah N Qamar, Stephen P Luby, Gordon Dougan, Zoe A Dyson, Stephen Baker, Denise O Garrett, Jason R Andrews, Samir K Saha, Senjuti Saha
Jan 27, 2023
Salmonella Paratyphi A, the primary etiology of paratyphoid, is estimated to cause 3.4 million infections annually, worldwide. With rising antimicrobial resistance and no licensed vaccines, genomic surveillance is key to track...
Diagnostic metabolite biomarkers of chronic typhoid carriage.
Elin Näsström, Pär Jonsson, Anders Johansson, Sabina Dongol, Abhilasha Karkey, Buddha Basnyat, Nga Tran Vu Thieu, Tan Trinh Van, Guy E Thwaites, Henrik Antti, Stephen Baker
Apr 10, 2019
BACKGROUND: Salmonella Typhi and Salmonella Paratyphi A are the agents of enteric (typhoid) fever; both can establish chronic carriage in the gallbladder. Chronic Salmonella carriers are typically asymptomatic, intermittently...
A 23-year retrospective investigation of Salmonella Typhi and Salmonella Paratyphi isolated in a tertiary Kathmandu hospital.
Raphaël M Zellweger, Buddha Basnyat, Poojan Shrestha, Krishna G Prajapati, Sabina Dongol, Paban K Sharma, Samir Koirala, Thomas C Darton, Christiane Dolecek, Corinne N Thompson, Guy E Thwaites, Stephen G Baker, Abhilasha Karkey
Feb 19, 2019
BACKGROUND: Salmonella serovars Typhi (S. Typhi) and Paratyphi A (S. Paratyphi A), the causative agents of enteric fever, have been routinely isolated organisms from the blood of febrile patients in the Kathmandu Valley since...