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Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput.
Josephin M Holstein
,
Christian Gylstorff
,
Florian Hollfelder
Jan 21, 2021
Compartmentalization of single genes in water-in-oil emulsion droplets is a powerful approach to create millions of reactors for enzyme library selections. When these droplets are formed at ultrahigh throughput in microfluidic...
cell-free protein synthesis
directed evolution
Microfluidics
Protein Engineering
ultrahigh throughput
Bacillus
Bacterial Proteins
Base Sequence
DNA
Bacterial
Directed Molecular Evolution
Emulsions
Escherichia coli
Genes
Bacterial
High-Throughput Screening Assays
Microfluidic Analytical Techniques
Microfluidics
Nucleic Acid Amplification Techniques
Plasmids
Protein Engineering
Serine Endopeptidases
Workflow
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