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Regeneration capacity is reduced as CNS axons mature. Using laser-mediated axotomy, proteomics and puromycin-based tagging of newly-synthesized proteins in a human embryonic stem cell-derived neuron culture system that allows...

Axon regeneration Axotomy Human stem cells In vitro live imaging local translation Proteomics Axons Cellular Senescence Coculture Techniques Embryonic Stem Cells Humans Nerve Regeneration Protein Biosynthesis

Long-distance RNA transport enables local protein synthesis at metabolicallyactive sites distant from the nucleus. This process ensures an appropriate spatial organization of proteins, vital to polarized cells such as neurons....

ANXA11 Amyotrophic Lateral Sclerosis local translation lysosome neurodegeneration neuron Organelles contact phase separation RNA granule RNA transport Amyotrophic Lateral Sclerosis Animals Animals Genetically Modified Annexins Axonal Transport Axons Cell Line Tumor Cytoplasmic Granules Female Humans Induced Pluripotent Stem Cells Lysosomes Male Mutation Protein Binding RNA Rats Rats Sprague-Dawley Transfection Zebrafish

miRNAs local translation growth cone Axon guidance brain wiring miR-182 Slit2 cofilin

Messenger RNA (mRNA) localization allows spatiotemporal regulation of the proteome at the subcellular level. This is observed in the axons of neurons, where mRNA localization is involved in regulating neuronal development and...

RNA-Binding Proteins axon local translation mRNA trafficking neuron Animals Axons Binding Sites Gene Expression Profiling Gene Expression Regulation Humans Neurons Protein Transport RNA transport RNA Messenger RNA-Binding Proteins Response Elements Transcriptome

Rps4x axon axon branching axonal protein synthesis local translation mRNA neural wiring Ribosomal Proteins ribosome ribosome remodeling Animals Axons Brain Cells Cultured Neurogenesis RNA Messenger Regulatory Sequences Ribonucleic Acid Ribosomal Proteins Ribosomes Xenopus laevis

Local translation is rapidly regulated by extrinsic signals during neural wiring, but its control mechanisms remain elusive. Here we show that the extracellular cue Sema3A induces an initial burst in local translation that...

PERK Sema3A axon axon branching Axon guidance eIF2B eIF2α local translation Retinal ganglion cell Unfolded Protein Response Animals Axons Eukaryotic Initiation Factor-2 Eukaryotic Initiation Factor-2B Female Male Neurogenesis Phosphorylation Protein Interaction Maps Proteomics Retinal Ganglion Cells Semaphorin-3A Signal Transduction Tissue Culture Techniques Xenopus Proteins Xenopus laevis eIF-2 Kinase