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ROS Thioflavin T fluorophores protein aggregation Sensors single-molecule imaging

How G protein-coupled receptors (GPCRs) are organized at the cell surface remains highly contentious. Single-molecule (SM) imaging is starting to inform this debate as receptor behavior can now be visualized directly, without...

BRET G protein-coupled receptors single-molecule imaging Animals Humans Ligands Protein Subunits Receptors G-Protein-Coupled single molecule imaging

T cell receptor dwell time microvilli receptor triggering single-molecule imaging Animals Antigen-Presenting Cells Host-Pathogen Interactions Humans Immunity Innate Kinetics Ligands Lymphocyte Activation Major Histocompatibility Complex Microvilli Models Theoretical Peptides Phosphorylation Receptors Antigen T-Cell Signal Transduction single molecule imaging T-Lymphocytes

Points for accumulation in nanoscale topography (PAINT) allows practically unlimited measurements in localisation microscopy but is limited by background fluorescence at high probe concentrations, especially in volumetric...

biophysics Localisation Microscopy Paint single-molecule imaging super-resolution microscopy DNA Imaging Three-Dimensional Membrane Proteins Microscopy Fluorescence single molecule imaging

The organization of Rhodopsin-family G protein-coupled receptors (GPCRs) at the cell surface is controversial. Support both for and against the existence of dimers has been obtained in studies of mostly individual receptors....

BRET G protein-coupled receptors evolution single-molecule imaging stoichiometry Fluorescence Resonance Energy Transfer HEK293 Cells Humans Protein Multimerization Protein Structure Quaternary Protein Structure Secondary Receptors G-Protein-Coupled Rhodopsin