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Development of a Clinical MALDI-ToF Mass Spectrometry Assay for SARS-CoV-2
Ray K Iles, Raminta Zmuidinaite, Jason K Iles, George Carnell, Alex Sampson, Jonathan L Heeney
Dec 14, 2020
The COVID-19 pandemic caused by the SARS-CoV-2 coronavirus has stretched national testing capacities to breaking points in almost all countries of the world. The need to rapidly screen vast numbers of a country's population in...
A How to Guide
Applying MALDI-ToF mass spectrometry as a clinical diagnostic test for viruses is different from that of bacteria, fungi and other micro-organisms. This is because the systems biology of viral infections, the size and chemical...
Development of a Clinical MALDI-ToF Mass Spectrometry Assay for SARS-CoV-2
Ray K. Iles, Raminta Zmuidinaite, Jason K. Iles, George Carnell, Alex Sampson, Jonathan L. Heeney
Sep 25, 2020
The COVID-19 pandemic caused by the SARS-CoV-2 coronavirus has stretched national testing capacities to breaking points in almost all countries of the world. The need to rapidly screen vast numbers of a country’s population in...
Inexpensive and fast pathogenic bacteria screening using field-effect transistors
Nello Formisano, Nikhil Bhalla, Mel Heeran, Juana Reyes Martinez, Amrita Sarkar, Maisem Laabei, Pawan Jolly, Chris R. Bowen, John T. Taylor, Sabine Flitsch, Pedro Estrela
Nov 15, 2016
While pathogenic bacteria contribute to a large number of globally important diseases and infections, current clinical diagnosis is based on processes that last a few days, employing complex and expensive techniques. Therefore...
SARS-CoV-2 Spike Protein Binding of Glycated Serum Albumin-Its Potential Role in the Pathogenesis of the COVID-19 Clinical Syndromes and Bias towards Individuals with Pre-Diabetes/Type 2 Diabetes and Metabolic Diseases.
Jason Iles, Raminta Zmuidinaite, Christoph Sadee, Anna Gardiner, Jonathan Lacey, Stephen Harding, Jernej Ule, Debra Roblett, Jonathan Heeney, Helen Baxendale, Ray K Iles
May 24, 2022
The immune response to SARS-CoV-2 infection requires antibody recognition of the spike protein. In a study designed to examine the molecular features of anti-spike and anti-nucleocapsid antibodies, patient plasma proteins...
Characterisation of FUT4 and FUT6 α-(1 → 2)-fucosyltransferases reveals that absence of root arabinogalactan fucosylation increases Arabidopsis root growth salt sensitivity.
Theodora Tryfona, Tina E Theys, Tanya Wagner, Katherine Stott, Kenneth Keegstra, Paul Dupree
Jan 16, 2018
Plant type II arabinogalactan (AG) polysaccharides are attached to arabinogalactan proteins (AGPs) at hydroxyproline residues, and they are very diverse and heterogeneous structures. The AG consists of a β-(1 → 3)-linked...
Inexpensive and fast pathogenic bacteria screening using field-effect transistors
Nello Formisano, Nikhil Bhalla, Mel Heeran, Juana Reyes Martinez, Amrita Sarkar, Maisem Laabei, Pawan Jolly, Chris R. Bowen, John T. Taylor, Sabine Flitsch, Pedro Estrela
Nov 15, 2016
While pathogenic bacteria contribute to a large number of globally important diseases and infections, current clinical diagnosis is based on processes that last a few days, employing complex and expensive techniques. Therefore...
SARS-CoV-2 Spike Protein Binding of Glycated Serum Albumin-Its Potential Role in the Pathogenesis of the COVID-19 Clinical Syndromes and Bias towards Individuals with Pre-Diabetes/Type 2 Diabetes and Metabolic Diseases.
Jason Iles, Raminta Zmuidinaite, Christoph Sadee, Anna Gardiner, Jonathan Lacey, Stephen Harding, Jernej Ule, Debra Roblett, Jonathan Heeney, Helen Baxendale, Ray K Iles
Apr 09, 2022
The immune response to SARS-CoV-2 infection requires antibody recognition of the spike protein. In a study designed to examine the molecular features of anti-spike and anti-nucleocapsid antibodies, patient plasma proteins...
PIK3R1W624R Is an Actionable Mutation in High Grade Serous Ovarian Carcinoma.
Concetta D'Ambrosio, Jessica Erriquez, Maddalena Arigoni, Sonia Capellero, Gloria Mittica, Eleonora Ghisoni, Fulvio Borella, Dionyssios Katsaros, Silvana Privitera, Marisa Ribotta, Elena Maldi, Giovanna Di Nardo, Enrico Berrino, Tiziana Venesio, Riccardo Ponzone, Marco Vaira, Douglas Hall, Mercedes Jimenez-Linan, Anna L Paterson, Raffaele A Calogero, James D Brenton, Giorgio Valabrega, Maria Flavia Di Renzo, Martina Olivero
Mar 10, 2020
Identifying cancer drivers and actionable mutations is critical for precision oncology. In epithelial ovarian cancer (EOC) the majority of mutations lack biological or clinical validation. We fully characterized 43 lines of...
PDX derived tumour cells PI3K PIK3R1 Patient-Derived xenografts ovarian cancer Animals Carcinoma Ovarian Epithelial Cell Line Tumor Class Ia Phosphatidylinositol 3-Kinase Cystadenocarcinoma Serous Female Heterografts Humans Mice Mice Inbred NOD Mice SCID Molecular Targeted Therapy Mutation Neoplasm Grading Ovarian Neoplasms Random Allocation
Determination of IgG1 and IgG3 SARS-CoV-2 Spike Protein and Nucleocapsid Binding-Who Is Binding Who and Why?
Jason K Iles, Raminta Zmuidinaite, Christoph Sadee, Anna Gardiner, Jonathan Lacey, Stephen Harding, Gregg Wallis, Roshani Patel, Debra Roblett, Jonathan Heeney, Helen Baxendale, Ray Kruse Iles
Jul 10, 2022
The involvement of immunoglobulin (Ig) G3 in the humoral immune response to SARS-CoV-2 infection has been implicated in the pathogenesis of acute respiratory distress syndrome (ARDS) in COVID-19. The exact molecular mechanism is...
Precursor transformation during molecular oxidation catalysis with organometallic iridium complexes
Ulrich Hintermair, Stafford W. Sheehan, Alexander R. Parent, Daniel H. Ess, David T. Richens, Patrick H. Vaccaro, Gary W. Brudvig, Robert H. Crabtree
Jul 24, 2013
We present evidence for Cp* being a sacrificial placeholder ligand in the [Cp*IrIII(chelate)X] series of homogeneous oxidation catalysts. UV-vis and 1H-NMR profiles as well as MALDI-MS data show a rapid and irreversible loss of...
PIK3R1 W624R Is an Actionable Mutation in High Grade Serous Ovarian Carcinoma
D’Ambrosio, Erriquez, Arigoni, Capellero, Mittica, Ghisoni, Borella, Katsaros, Privitera, Ribotta, Maldi, Di Nardo, Berrino, Venesio, Ponzone, Vaira, Hall, Jimenez-Linan, Paterson, Calogero, Brenton, Valabrega, Di Renzo, Olivero
Feb 16, 2020
Identifying cancer drivers and actionable mutations is critical for precision oncology. In epithelial ovarian cancer (EOC) the majority of mutations lack biological or clinical validation. We fully characterized 43 lines of...
Ruthenium(II) dichloro or dithiocyanato complexes with 4,4′:2′,2″:4″,4‴-quaterpyridinium ligands
O.A. Blackburn, B.J. Coe, M. Helliwell, Y.T. Ta, L.M. Peter, H. Wang, J.A. Anta, E. Guillén
Feb 13, 2013
Fourteen new complexes of the form cis-[Ru IIX 2(R 2qpy 2+) 2] 4+ (R 2qpy 2+ = a 4,4′:2′,2″:4″,4‴-quaterpyridinium ligand, X = Cl - or NCS -) have been prepared and isolated as their PF 6 - salts. Characterisation involved...
Ruthenium(II) dichloro or dithiocyanato complexes with 4,4′:2′,2″:4″,4‴-quaterpyridinium ligands
O.A. Blackburn, B.J. Coe, M. Helliwell, Y.T. Ta, L.M. Peter, H. Wang, J.A. Anta, E. Guillén
Feb 13, 2013
Fourteen new complexes of the form cis-[Ru IIX 2(R 2qpy 2+) 2] 4+ (R 2qpy 2+ = a 4,4′:2′,2″:4″,4‴-quaterpyridinium ligand, X = Cl - or NCS -) have been prepared and isolated as their PF 6 - salts. Characterisation involved...
Precursor transformation during molecular oxidation catalysis with organometallic iridium complexes
Ulrich Hintermair, Stafford W. Sheehan, Alexander R. Parent, Daniel H. Ess, David T. Richens, Patrick H. Vaccaro, Gary W. Brudvig, Robert H. Crabtree
Jul 24, 2013
We present evidence for Cp* being a sacrificial placeholder ligand in the [Cp*IrIII(chelate)X] series of homogeneous oxidation catalysts. UV-vis and 1H-NMR profiles as well as MALDI-MS data show a rapid and irreversible loss of...
Determination of IgG1 and IgG3 SARS-CoV-2 Spike Protein and Nucleocapsid Binding-Who Is Binding Who and Why?
Jason K Iles, Raminta Zmuidinaite, Christoph Sadee, Anna Gardiner, Jonathan Lacey, Stephen Harding, Gregg Wallis, Roshani Patel, Debra Roblett, Jonathan Heeney, Helen Baxendale, Ray Kruse Iles
May 30, 2022
The involvement of immunoglobulin (Ig) G3 in the humoral immune response to SARS-CoV-2 infection has been implicated in the pathogenesis of acute respiratory distress syndrome (ARDS) in COVID-19. The exact molecular mechanism is...
Protein Characterization by Mass Spectrometry
Steven Louis Cohen
Jan 01, 0001
Powerful new techniques in mass spectrometry (MS) provide an unprecedented opportunity to develop methods that facilitate protein characterization. This thesis utilizes matrix-assisted laser desorption/ionization and...
Published by: Rockefeller University