Search
Results: 519
Advanced fluorescence imaging of in situ protein aggregation.
The aggregation of intrinsically disordered proteins is a hallmark of neurodegenerative diseases, such as Alzheimer's, Parkinson's and Huntington's disease. Although we currently have a good molecular level understanding on how...
Biophotonics of Native Silk Fibrils.
Ulyana Shimanovich, Dorothea Pinotsi, Klimentiy Shimanovich, Na Yu, Sreenath Bolisetty, Jozef Adamcik, Raffaele Mezzenga, Jerome Charmet, Fritz Vollrath, Ehud Gazit, Christopher M Dobson, Gabriele Kaminski Schierle, Chris Holland, Clemens F Kaminski, Tuomas PJ Knowles
May 07, 2019
Machine learning assisted interferometric structured illumination microscopy for dynamic biological imaging.
Edward N Ward, Lisa Hecker, Charles N Christensen, Jacob R Lamb, Meng Lu, Luca Mascheroni, Chyi Wei Chung, Anna Wang, Christopher J Rowlands, Gabriele S Kaminski Schierle, Clemens F Kaminski
Jan 22, 2023
Structured Illumination Microscopy, SIM, is one of the most powerful optical imaging methods available to visualize biological environments at subcellular resolution. Its limitations stem from a difficulty of imaging in multiple...
Machine learning assisted interferometric structured illumination microscopy for dynamic biological imaging.
From single-molecule spectroscopy to super-resolution imaging of the neuron
Romain F Laine, Gabriele S Kaminski Schierle, Sebastian van de Linde, Clemens F Kaminski
May 10, 2016
Structural progression of amyloid-β Arctic mutant aggregation in cells revealed by multiparametric imaging.
Meng Lu, Neil Williamson, Ajay Mishra, Claire Michel, Clemens Kaminski, Alan Tunnacliffe, Gabriele Kaminski
Jan 02, 2019
An Easy-to-Implement Protocol for Preparing Postnatal Ventral Mesencephalic Cultures.
Janin Lautenschläger, Eugene V Mosharov, Ellen Kanter, David Sulzer, Gabriele S Kaminski Schierle
Apr 19, 2018
Postnatally derived cultures of ventral mesencephalic neurons offer several crucial advantages over embryonic ventral mesencephalic cultures, including a higher content of TH-positive cells and the ability to derive cells from...
Mitochondrial degradation of amyloidogenic proteins - A new perspective for neurodegenerative diseases.
This perspective article outlines mechanisms of mitochondrial import and protein degradation and how these have been linked to alpha-synuclein and Amyloid beta (Aβ) homeostasis. Our aim is to underpin and stimulate the debate on...
A label-free, quantitative assay of amyloid fibril growth based on intrinsic fluorescence.
Dorothea Pinotsi, Alexander K Buell, Christopher M Dobson, Gabriele S Kaminski Schierle, Clemens F Kaminski
Feb 12, 2020
Kinetic assay of seeded growth: The graph shows the variation in intrinsic fluorescence intensity of amyloid fibrils. Fluorescence increases during the seeded aggregation of α-synuclein seeds with α-synuclein monomeric protein...
Sea Cucumber-Derived Peptides Alleviate Oxidative Stress in Neuroblastoma Cells and Improve Survival in <i>C. elegans</i> Exposed to Neurotoxic Paraquat.
Meng Lu, Ajay Mishra, Chiara Boschetti, Jing Lin, Yushuang Liu, Hongliang Huang, Clemens Kaminski, Zebo Huang, Alan Tunnacliffe, Gabriele Kaminski
Jun 17, 2021
Published by: Oxidative medicine and cellular longevity
Purification of Recombinant α-synuclein
Amberley D Stephens, Dijana Matak-Vinkovic, Ana Fernandez-Villegas, Gabriele S Kaminski Schierle
Nov 23, 2020
The initial state of the intrinsically disordered protein α-synuclein (aSyn), e.g., the presence of oligomers and degradation products, or the presence of contaminants and adducts can greatly influence the aggregation kinetics...
Cell Line Cell Survival Chemical Precipitation Chromatography Gel Chromatography Ion Exchange Chromatography Liquid Escherichia coli Humans Intrinsically Disordered Proteins Microscopy Electron Transmission Protein Aggregates Protein Conformation Protein Conformation beta-Strand Recombinant Proteins Spectrometry Mass Electrospray Ionization ALPHA-SYNUCLEIN
Design of a Functionalized Metal-Organic Framework System for Enhanced Targeted Delivery to Mitochondria.
Salame Haddad, Isabel Abánades Lázaro, Marcus Fantham, Ajay Mishra, Joaquin Silvestre-Albero, Johannes W M Osterrieth, Gabriele S Kaminski Schierle, Clemens F Kaminski, Ross S Forgan, David Fairen-Jimenez
Apr 17, 2020
Mitochondria play a key role in oncogenesis and constitute one of the most important targets for cancer treatments. Although the most effective way to deliver drugs to mitochondria is by covalently linking them to a lipophilic...
Published by: Journal of the American Chemical Society
Structural basis of synaptic vesicle assembly promoted by α-synuclein.
Giuliana Fusco, Tillmann Pape, Amberley D Stephens, Pierre Mahou, Ana Rita Costa, Clemens F Kaminski, Gabriele S Kaminski Schierle, Michele Vendruscolo, Gianluigi Veglia, Christopher M Dobson, Alfonso De Simone
Mar 04, 2020
α-synuclein (αS) is an intrinsically disordered protein whose fibrillar aggregates are the major constituents of Lewy bodies in Parkinson's disease. Although the specific function of αS is still unclear, a general consensus is...
Direct observation of heterogeneous amyloid fibril growth kinetics via two-color super-resolution microscopy.
Dorothea Pinotsi, Alexander K Buell, Celine Galvagnion, Christopher M Dobson, Gabriele S Kaminski Schierle, Clemens F Kaminski
Feb 12, 2020
The self-assembly of normally soluble proteins into fibrillar amyloid structures is associated with a range of neurodegenerative disorders, such as Parkinson's and Alzheimer's diseases. In the present study, we show that...
Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells.
Miranda Robbins, Venkat Pisupati, Roberta Azzarelli, Samer I Nehme, Roger Barker, Ljiljana Fruk, Gabriele S Kaminski Schierle
Nov 22, 2021
Proton Transfer and Structure-Specific Fluorescence in Hydrogen Bond-Rich Protein Structures.
Dorothea Pinotsi, Luca Grisanti, Pierre Mahou, Ralph Gebauer, Clemens F Kaminski, Ali Hassanali, Gabriele S Kaminski Schierle
Feb 03, 2016
An Expanded Polyproline Domain Maintains Mutant Huntingtin Soluble in vivo and During Aging.
Maria Lucia Pigazzini, Mandy Lawrenz, Anca Margineanu, Gabriele S Kaminski Schierle, Janine Kirstein
Jan 06, 2022
|
|< |
< |
1 |