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Genetics and immunity in the era of single-cell genomics.
Recent developments in the field of single-cell genomics (SCG) are changing our understanding of how functional phenotypes of cell populations emerge from the behaviour of individual cells. Some of the applications of SCG...
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Peer-to-peer energy trading in a microgrid leveraged by smart contracts
Guilherme Vieira, Jie Zhang
Jun 30, 2021
The current electricity networks were not initially designed for the high integration of variable generation technologies. They suffer significant losses due to the combustion of fossil fuels, the long-distance transmission, and...
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Direitos Sociais na Constituição Uma Análise da Constitucionalização dos Direitos Sociais em Portugal, 1975-76
Embora todas as constituições incluam direitos, e muitas delas incluam direitos sociais, a verdade é que algumas são mais generosas do que outras a este respeito. Mas nenhuma se aproxima da Constituição da República Portuguesa...
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Effects of TDP2/VPg Unlinkase Activity on Picornavirus Infections Downstream of Virus Translation
In this study, we characterized the role of host cell protein tyrosyl-DNA phosphodiesterase 2 (TDP2) activity, also known as VPg unlinkase, in picornavirus infections in a human cell model of infection. TDP2/VPg unlinkase is...
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Peer-to-peer energy trading in a microgrid leveraged by smart contracts
Guilherme Vieira, Jie Zhang
Jun 30, 2021
The current electricity networks were not initially designed for the high integration of variable generation technologies. They suffer significant losses due to the combustion of fossil fuels, the long-distance transmission, and...
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Changes in physical activity during the retirement transition
BACKGROUND: Existing evidence about the impact of retirement on physical activity (PA) has primarily focused on the average change in PA level after retirement in group-based studies. It is unclear whether findings regarding the...
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Are cells from a snowman realistic? Cryopreserved tissues as a source for single-cell RNA-sequencing experiments.
A recently published study in Genome Biology shows that cells isolated from cryopreserved tissues are a reliable source of genetic material for single-cell RNA-sequencing experiments.Please see related Method article...
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Reliable measurement of free Ca2+ concentrations in the ER lumen using Mag-Fluo-4.
Ana M Rossi, Colin W Taylor
Mar 02, 2020
Synthetic Ca2+ indicators are widely used to report changes in free [Ca2+], usually in the cytosol but also within organelles. Mag-Fluo-4, loaded into the endoplasmic reticulum (ER) by incubating cells with Mag-Fluo-4 AM, has...
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Analyses of Ligand Binding to IP3 Receptors Using Fluorescence Polarization.
Ana M Rossi, Colin W Taylor
Jan 16, 2019
Fluorescence polarization (FP) can be used to measure binding of a small fluorescent ligand to a larger protein because the ligand rotates more rapidly in its free form than when bound. When excited with plane polarized light...
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Repurposing Drugs in Small Animal Oncology.
Repurposing drugs in oncology consists of using off-label drugs that are licensed for various non-oncological medical conditions to treat cancer. Repurposing drugs has the advantage of using drugs that are already...
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Green Finance or Daltonic Finance?
Clean energy is currently a top priority on the global agenda, and green bonds have emerged as a key response from financial markets. However, while these bonds aim to reduce carbon emissions, they may create perverse...

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