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A simple umbelliferone based fluorescent probe for the detection of nitroreductase

OAI: oai:purehost.bath.ac.uk:publications/273f8e7e-e8e6-43c8-b92d-7341751be648 • DOI: https://doi.org/10.1007/s11705-017-1697-0
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Abstract

A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR. [Figure not available: see fulltext.]