Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems.

Gary S McDowell, Anna Philpott

OAI: oai:www.repository.cam.ac.uk:1810/280349 • DOI: 10.17863/CAM.27721

The Xenopus extract system has been used extensively as a simple, quick, and robust method for assessing the stability of proteins against proteasomal degradation. In this protocol, methods are provided for assessing the half-life of in vitro translated radiolabeled proteins using Xenopus egg or embryo extracts.

Animals Biochemistry Biological Assay Cell Extracts Protein Biosynthesis Proteolysis Radiopharmaceuticals Transcription Genetic Xenopus Proteins Xenopus laevis