Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications.

Loriana Vitillo, Ludovic Vallier

OAI: oai:www.repository.cam.ac.uk:1810/322578 • DOI: 10.17863/CAM.70035

Long-term neuroepithelial-like stem cells (lt-NES) derived from human embryonic stem cells are a stable self-renewing progenitor population with high neurogenic potential and phenotypic plasticity. Lt-NES are amenable to regional patterning toward neurons and glia subtypes and thus represent a valuable source of cells for many biomedical applications. For use in regenerative medicine and cell therapy, lt-NES and their progeny require derivation with high-quality culture conditions suitable for clinical use. In this chapter, we describe a robust method to derive multipotent and expandable lt-NES based on good manufacturing practice and cell therapy-grade reagents. We further describe fully defined protocols to terminally differentiate lt-NES toward GABA-ergic, dopaminergic, and motor neurons.

Cell therapy DIFFERENTIATION GMP Human Embryonic Stem Cells Lt-NES neural progenitors Regenerative Medicine Stem Cells Cell Differentiation Cell- and Tissue-Based Therapy Human Embryonic Stem Cells Humans Neural Stem Cells Neurogenesis