A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin <em>α_vβ₃in vitro</em>

Zhiyuan Hu, Rory L Arrowsmith, James A Tyson, Vincenzo Mirabello, Haobo Ge, Ian M Eggleston, Stanley W Botchway, Dan Pantos, Sofia I Pascu

OAI: oai:purehost.bath.ac.uk:openaire_cris_publications/dc16f90d-1b93-46b6-9658-710459af7051 • DOI: https://doi.org/10.1039/c4cc08265f

We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.

/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being