Divergent signalling pathways regulate lipopolysaccharide-induced eRNA expression in human monocytic THP1 cells

James A. Heward, Benoit T. Roux, Mark A. Lindsay

OAI: oai:purehost.bath.ac.uk:publications/f298dc89-b6ce-4bb0-ba7a-9f7d0dc54be5 • DOI: https://doi.org/10.1016/j.febslet.2014.12.026

Recent studies have indicated that non-coding RNAs transcribed from enhancer regions are important regulators of enhancer function and gene expression. In this report, we have characterised the expression of six enhancer RNAs (eRNAs) induced in human monocytic THP1 cells following activation of the innate immune response by lipopolysaccharide (LPS). Specifically, we have demonstrated that LPS-induced expression of individual eRNAs is mediated through divergent intracellular signalling pathways that includes NF-κB and the mitogen activated protein kinases, extracellular regulated kinase-1/2 and p38.

Enhancer RNA ERK and p38 LIPOPOLYSACCHARIDE long non-coding RNA monocyte NF-κB