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Results: 50
Integrative epigenomics, transcriptomics and proteomics of patient chondrocytes reveal genes and pathways involved in osteoarthritis.
Julia Steinberg, Graham RS Ritchie, Theodoros I Roumeliotis, Raveen L Jayasuriya, Matthew J Clark, Roger A Brooks, Abbie LA Binch, Karan M Shah, Rachael Coyle, Mercedes Pardo, Christine L Le Maitre, Yolande FM Ramos, Rob GHH Nelissen, Ingrid Meulenbelt, Andrew W McCaskie, Jyoti S Choudhary, J Mark Wilkinson, Eleftheria Zeggini
Dec 05, 2017
Aquaporin 1 Arthroplasty Replacement Hip Arthroplasty Replacement Knee Case-Control Studies Chondrocytes Chromatography Liquid Collagen Type I Collagen Type I alpha 1 Chain DNA Methylation Disease Progression Epigenesis Genetic Epigenomics Gene Expression Profiling Gene Regulatory Networks Humans Lectins C-Type Male Mass Spectrometry Osteoarthritis Hip Osteoarthritis Knee Proteomics Sequence Analysis RNA
Liquid Extraction Surface Analysis Mass Spectrometry Method for Identifying the Presence and Severity of Nonalcoholic Fatty Liver Disease
Functionalized graphene oxide tablets for sample preparation of drugs in biological fluids
Zeynab Zohdijamil, Mahdi Hashemi, Abbi Abdel-Rehim, Karthik Laxman, Abdusalam Uheida, Joydeep Dutta, Mohamed Abdel-Rehim
Jun 04, 2021
In this work, graphene oxide-based tablets (GO-Tabs) were prepared by applying a thin layer of functionalized GO on a polyethylene substrate. The GO was functionalized with amine groups (-NH2 ) by poly(ethylene...
Organoid Sample Preparation and Extraction for LC-MS Peptidomics.
Emily L Miedzybrodzka, Rachel E Foreman, Sam G Galvin, Pierre Larraufie, Amy L George, Deborah A Goldspink, Frank Reimann, Fiona M Gribble, Richard G Kay
Mar 18, 2021
This protocol describes the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry (LC-MS). Currently, most peptides are quantified by ELISA...
Tailored Mobility in a Zeolite Imidazolate Framework (ZIF) Antibody Conjugate*.
Ander Chapartegui-Arias, Anna Raysyan, Ana M Belenguer, Carsten Jaeger, Teodor Tchipilov, Carsten Prinz, Carlos Abad, Sebastian Beyer, Rudolf J Schneider, Franziska Emmerling
May 21, 2021
Zeolitic imidazolate framework (ZIF) hybrid fluorescent nanoparticles and ZIF antibody conjugates have been synthesized, characterized, and employed in lateral-flow immunoassay (LFIA). The bright fluorescence of the conjugates...
Comparative analysis of Erk phosphorylation suggests a mixed strategy for measuring phospho-form distributions.
Sudhakaran Prabakaran, Robert A Everley, Isabelle Landrieu, Jean-Michel Wieruszeski, Guy Lippens, Hanno Steen, Jeremy Gunawardena
Apr 19, 2018
The functional impact of multisite protein phosphorylation can depend on both the numbers and the positions of phosphorylated sites-the global pattern of phosphorylation or 'phospho-form'-giving biological systems profound...
Efficient and irreversible antibody-cysteine bioconjugation using carbonylacrylic reagents.
Barbara Bernardim, Maria J Matos, Xhenti Ferhati, Ismael Compañón, Ana Guerreiro, Padma Akkapeddi, Antonio CB Burtoloso, Gonzalo Jiménez-Osés, Francisco Corzana, Gonçalo JL Bernardes
May 08, 2019
Measurement of the total angiotensinogen and its reduced and oxidised forms in human plasma using targeted LC-MS/MS.
Lina A Dahabiyeh, David Tooth, Robin W Carrell, Randy J Read, Yahui Yan, Fiona Broughton Pipkin, David A Barrett
Dec 11, 2018
Angiotensinogen (AGT) is a critical protein in the renin-angiotensin-aldosterone system and may have an important role in the pathogenesis of pre-eclampsia. The disulphide linkage between cysteines 18 and 138 has a key role in...
Behavioural and molecular endophenotypes in psychotic disorders reveal heritable abnormalities in glutamatergic neurotransmission.
L Scoriels, RM Salek, E Goodby, D Grainger, AM Dean, JA West, JL Griffin, J Suckling, PJ Nathan, BR Lennox, GK Murray, ET Bullmore, PB Jones
May 04, 2015
Adult Analysis of Variance Chromatography Liquid Endophenotypes Excitatory Amino Acids Female Genetic Predisposition to Disease Glutamic Acid Humans Magnetic Resonance Spectroscopy Male Mass Spectrometry Metabolomics Neuropsychological Tests principal component analysis Psychotic Disorders Reaction Time Receptors N-Methyl-D-Aspartate Synaptic Transmission Young Adult
A sensitive mass spectrometric assay for mitochondrial CoQ pool redox state in vivo.
Nils Burger, Angela Logan, Tracy A Prime, Amin Mottahedin, Stuart T Caldwell, Thomas Krieg, Richard C Hartley, Andrew M James, Michael P Murphy
Oct 07, 2021
Coenzyme Q (CoQ) is an essential cofactor, primarily found in the mitochondrial inner membrane where it functions as an electron carrier in the respiratory chain, and as a lipophilic antioxidant. The redox state of the CoQ pool...
Label-Free Protein Analysis Using Liquid Chromatography with Gravimetric Detection.
Tadas Kartanas, Aviad Levin, Zenon Toprakcioglu, Tom Scheidt, Tuuli A Hakala, Jerome Charmet, Tuomas PJ Knowles
Jan 28, 2021
The Dissociation of Gefitinib Trough Concentration and Clinical Outcome in NSCLC Patients with EGFR Sensitive Mutations.
Shuang Xin, Yuanyuan Zhao, Xueding Wang, Yan Huang, Jing Zhang, Ying Guo, Jiali Li, Hongliang Li, Yuxiang Ma, Lingyan Chen, Zhihuang Hu, Min Huang, Li Zhang
Nov 26, 2019
Gefitinib is an essential drug for NSCLC patients harboring EGFR sensitive mutations. The approved dose 250 mg/day is based on limited clinical trials, this research aims to explore the relationship between drug exposure and...
Leptin-Mediated Changes in the Human Metabolome.
Katherine Lawler, Isabel Huang-Doran, Takuhiro Sonoyama, Tinh-Hai Collet, Julia M Keogh, Elana Henning, Stephen O'Rahilly, Leonardo Bottolo, I Sadaf Farooqi
May 15, 2020
CONTEXT: While severe obesity due to congenital leptin deficiency is rare, studies in patients before and after treatment with leptin can provide unique insights into the role that leptin plays in metabolic and endocrine...
bile acids Leptin Lipids Metabolomics obesity Adolescent Child Child Preschool Chromatography Liquid Energy Intake Energy Metabolism Female Hormone Replacement Therapy Humans Leptin Lipolysis Loss of Function Mutation Male Metabolome Metabolomics obesity Recombinant Proteins Severity of Illness Index Tandem Mass Spectrometry Treatment Outcome
Determination of the Synthetic Cannabinoids JWH-122, JWH-210, UR-144 in Oral Fluid of Consumers by GC-MS and Quantification of Parent Compounds and Metabolites by UHPLC-MS/MS.
Nunzia La Maida, Manuela Pellegrini, Esther Papaseit, Clara Pérez-Mañá, Lourdes Poyatos, Mireia Ventura, Liliana Galindo, Francesco Paolo Busardò, Simona Pichini, Magí Farré, Emilia Marchei
Dec 11, 2020
The consumption of synthetic cannabinoids (SCs) has significantly increased in the last decade and the analysis of SCs and their metabolites in human specimens is gaining interest in clinical and forensic toxicology. A pilot...
Quantitative mass spectrometry for human melanocortin peptides in vitro and in vivo suggests prominent roles for β-MSH and desacetyl α-MSH in energy homeostasis.
Peter Kirwan, Richard G Kay, Bas Brouwers, Vicente Herranz-Pérez, Magdalena Jura, Pierre Larraufie, Julie Jerber, Jason Pembroke, Theresa Bartels, Anne White, Fiona M Gribble, Frank Reimann, I Sadaf Farooqi, Stephen O'Rahilly, Florian T Merkle
Nov 13, 2018
OBJECTIVE: The lack of pro-opiomelanocortin (POMC)-derived melanocortin peptides results in hypoadrenalism and severe obesity in both humans and rodents that is treatable with synthetic melanocortins. However, there are...
Human pluripotent stem cell Leptin MSH Neuropeptide obesity POMC Chromatography Liquid Female Homeostasis Humans Hypothalamus Leptin Male Mass Spectrometry Melanocortins Neurons Neuropeptides Pluripotent Stem Cells Pro-Opiomelanocortin Receptors Melanocortin Tandem Mass Spectrometry alpha-MSH beta-MSH
Deciphering the genetic basis for polyketide variation among mycobacteria producing mycolactones.
Sacha J Pidot, Hui Hong, Torsten Seemann, Jessica L Porter, Marcus J Yip, Artem Men, Matthew Johnson, Peter Wilson, John K Davies, Peter F Leadlay, Timothy P Stinear
Jun 14, 2011
High-Resolution Mass Spectrometry Based Proteomic Analysis of the Response to Vancomycin-Induced Cell Wall Stress in Streptomyces coelicolor A3(2).
Involvement of a eukaryotic-like ubiquitin-related modifier in the proteasome pathway of the archaeon Sulfolobus acidocaldarius.
Rana S Anjum, Sian M Bray, John K Blackwood, Mairi L Kilkenny, Matthew A Coelho, Benjamin M Foster, Shurong Li, Julie A Howard, Luca Pellegrini, Sonja-Verena Albers, Michael J Deery, Nicholas P Robinson
Aug 04, 2015
β-Hydroxybutyrate Accumulates in the Rat Heart during Low-Flow Ischaemia with Implications for Functional Recovery
Ross Lindsay, Sophie Dieckmann, Dominika Krzyzanska, Dominic Manetta-Jones, James West, Cecilia Castro, Julian Griffin, Andrew Murray
Aug 24, 2021
Heart ischaemia Langendorff Biochemistry cardiomyocyte Cell Biology chemical biology rat 3-Hydroxybutyric Acid Animals Chromatography Liquid Citric Acid Cycle Heart Hydroxymethylglutaryl-CoA Synthase Ischemia ketone bodies Male Mitochondria Myocardial Ischemia Myocytes Cardiac Oxidation-Reduction Rats Rats Wistar Tandem Mass Spectrometry
A Platform for Site-Specific DNA-Antibody Bioconjugation by Using Benzoylacrylic-Labelled Oligonucleotides.
Juraj Konč, Libby Brown, Daniel R Whiten, Yukun Zuo, Peter Ravn, David Klenerman, Gonçalo JL Bernardes
Sep 28, 2021
Many bioconjugation strategies for DNA oligonucleotides and antibodies suffer limitations, such as site-specificity, stoichiometry and hydrolytic instability of the conjugates, which makes them unsuitable for biological...
Mass spectrometric characterisation of the circulating peptidome following oral glucose ingestion in control and gastrectomised patients.
Richard G Kay, Rachel E Foreman, Geoff P Roberts, Richard Hardwick, Frank Reimann, Fiona M Gribble
Jun 04, 2020
Biomarker panels associated with progression of renal disease in type 1 diabetes.
Marco Colombo, Erkka Valo, Stuart J McGurnaghan, Niina Sandholm, Luke AK Blackbourn, R Neil Dalton, David Dunger, Per-Henrik Groop, Paul M McKeigue, Carol Forsblom, Helen M Colhoun, FinnDiane Study Group and the Scottish Diabetes Research Network (SDRN) Type 1 Bioresource Collaboration
May 03, 2019
Palaeoproteomics confirm earliest domesticated sheep in southern Africa ca. 2000 BP.
Ashley N Coutu, Alberto J Taurozzi, Meaghan Mackie, Theis Zetner Trolle Jensen, Matthew J Collins, Judith Sealy
Oct 29, 2021
We used palaeoproteomics and peptide mass fingerprinting to obtain secure species identifications of key specimens of early domesticated fauna from South Africa, dating to ca. 2000 BP. It can be difficult to distinguish...
Bilirubin deficiency renders mice susceptible to hepatic steatosis in the absence of insulin resistance.
Weiyu Chen, Sergey Tumanov, Daniel J Fazakerley, James Cantley, David E James, Louise L Dunn, Taqi Shaik, Cacang Suarna, Roland Stocker
Nov 05, 2021
BACKGROUND & AIMS: Plasma concentrations of bilirubin, a product of heme catabolism formed by biliverdin reductase A (BVRA), inversely associate with the risk of metabolic diseases including hepatic steatosis and diabetes...
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