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Results: 304
Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Cadmium Compounds Codon Eukaryotic Initiation Factor-2 Fungal Proteins Hot Temperature Hydrogen Peroxide MAP Kinase Signaling System Methyl Methanesulfonate Mitogen-Activated Protein Kinases Osmotic Pressure Oxidative Stress Peptide Chain Elongation Translational RNA Fungal RNA Messenger RNA Transfer Trp Ribosomes Schizosaccharomyces Schizosaccharomyces pombe Proteins Sorbitol Sulfates Tryptophan
Thyroid Hormone Deficiency Suppresses Fetal Pituitary-Adrenal Function Near Term
Emily J Camm, Isabella Inzani, Miles J De Blasio, Katie L Davies, India R Lloyd, FB Peter Wooding, Dominique Blache, Abigail L Fowden, Alison J Forhead
Oct 22, 2020
ACTH adrenal gland corticotrophs Cortisol Fetus Pituitary Gland Thyroid Hormones Adrenal Cortex Hormones Adrenal Glands Adrenal Medulla Adrenocorticotropic Hormone Animals Cell Count Cell Proliferation congenital hypothyroidism Corticotrophs Fetal Development Fetal Diseases Fetal Organ Maturity Hydrocortisone Hypothalamo-Hypophyseal System Insulin-Like Growth Factor I Pituitary-Adrenal System RNA Messenger Receptor IGF Type 1 Sheep Thyroidectomy Thyroxine Triiodothyronine Zona Fasciculata
A novel method for poly(A) fractionation reveals a large population of mRNAs with a short poly(A) tail in mammalian cells.
Hedda A Meijer, Martin Bushell, Kirsti Hill, Timothy W Gant, Anne E Willis, Peter Jones, Cornelia H de Moor
Mar 01, 2021
The length of the poly(A) tail of an mRNA plays an important role in translational efficiency, mRNA stability and mRNA degradation. Regulated polyadenylation and deadenylation of specific mRNAs is involved in oogenesis...
Mechanisms of stretch-mediated skin expansion at single-cell resolution.
Mariaceleste Aragona, Alejandro Sifrim, Milan Malfait, Yura Song, Jens Van Herck, Sophie Dekoninck, Souhir Gargouri, Gaëlle Lapouge, Benjamin Swedlund, Christine Dubois, Pieter Baatsen, Katlijn Vints, Seungmin Han, Fadel Tissir, Thierry Voet, Benjamin D Simons, Cédric Blanpain
Jun 09, 2020
Adaptor Proteins Signal Transducing Adherens Junctions Animals Base Sequence Cell Cycle Proteins Cell Differentiation Cell Self Renewal Chromatin Chromatin Assembly and Disassembly Clone Cells Disease Models Animal Extracellular Signal-Regulated MAP Kinases Gene Regulatory Networks Hydrogels Mechanotransduction Cellular Mice Mice Transgenic Mitogen-Activated Protein Kinase Kinases Mutation RNA Messenger RNA-seq Single-Cell Analysis Skin Stem Cells Trans-Activators Transcription Factor AP-1 Transcription Genetic YAP-Signaling Proteins
The circadian clock components BMAL1 and REV-ERBα regulate flavivirus replication.
Xiaodong Zhuang, Andrea Magri, Michelle Hill, Alvina G Lai, Abhinav Kumar, Srinivasa Bhargav Rambhatla, Claire L Donald, Andrea F Lopez-Clavijo, Simon Rudge, Katherine Pinnick, Wai Hoong Chang, Peter AC Wing, Ryan Brown, Ximing Qin, Peter Simmonds, Thomas F Baumert, David Ray, Andrew Loudon, Peter Balfe, Michael Wakelam, Sam Butterworth, Alain Kohl, Catherine L Jopling, Nicole Zitzmann, Jane A McKeating
Jun 27, 2019
The circadian clock regulates immune responses to microbes and affects pathogen replication, but the underlying molecular mechanisms are not well understood. Here we demonstrate that the circadian components BMAL1 and REV-ERBα...
ARNTL Transcription Factors Cell Line Circadian Clocks DNA Replication dengue dengue virus Flavivirus Gene Expression Regulation Genes Essential Hepacivirus Hepatitis C Hepatocytes Humans Nuclear Receptor Subfamily 1 Group D Member 1 Proteomics RNA Messenger Virus Internalization Virus Replication Zika Virus Zika Virus Infection
RORα and 25-Hydroxycholesterol Crosstalk Regulates Lipid Droplet Homeostasis in Macrophages.
Zewen Kelvin Tuong, Patrick Lau, Ximing Du, Nicholas D Condon, Joel M Goode, Tae Gyu Oh, Jeremy C Yeo, George EO Muscat, Jennifer L Stow
Sep 02, 2019
Nuclear hormone receptors have important roles in the regulation of metabolic and inflammatory pathways. The retinoid-related orphan receptor alpha (Rorα)-deficient staggerer (sg/sg) mice display several phenotypes indicative of...
Animals Bone Marrow Cells Cells Cultured Chromatography Thin Layer Drug Inverse Agonism Hydroxycholesterols Lipid Droplets Lipid Metabolism Lipids MACROPHAGES Male Mice Mice Inbred C3H Mice Knockout Nuclear Receptor Subfamily 1 Group F Member 1 RNA Interference RNA Messenger Receptors LDL Steroid Hydroxylases Up-Regulation
Artificial miRNAs Reduce Human Mutant Huntingtin Throughout the Striatum in a Transgenic Sheep Model of Huntington's Disease.
Edith L Pfister, Natalie DiNardo, Erica Mondo, Florie Borel, Faith Conroy, Cara Fraser, Gwladys Gernoux, Xin Han, Danjing Hu, Emily Johnson, Lori Kennington, PengPeng Liu, Suzanne J Reid, Ellen Sapp, Petr Vodicka, Tim Kuchel, A Jennifer Morton, David Howland, Richard Moser, Miguel Sena-Esteves, Guangping Gao, Christian Mueller, Marian DiFiglia, Neil Aronin
Sep 20, 2018
Huntington's disease (HD) is a fatal neurodegenerative disease caused by a genetic expansion of the CAG repeat region in the huntingtin (HTT) gene. Studies in HD mouse models have shown that artificial miRNAs can reduce mutant...
AAV Huntington's disease RNAi large animal models Animals Animals Genetically Modified Dependovirus Disease Models Animal Electrolytes Genetic Vectors Genome Viral Humans Huntingtin Protein Huntington Disease Immunoassay Injections Kidney Liver MicroRNAs Microglia Mutant Proteins Neostriatum Neurons RNA Messenger Sheep
Injury-induced perivascular niche supports alternative differentiation of adult rodent CNS progenitor cells.
Justyna Ulanska-Poutanen, Jakub Mieczkowski, Chao Zhao, Katarzyna Konarzewska, Beata Kaza, Hartmut Bf Pohl, Lukasz Bugajski, Bozena Kaminska, Robin Jm Franklin, Malgorzata Zawadzka
Nov 19, 2018
Schwann Cells adult CNS progenitors neuroscience perivascular niche rat remyelination Animals Astrocytes Bone Morphogenetic Proteins Cell Differentiation Cellular Microenvironment Central Nervous System Demyelinating Diseases Endothelial Cells Gene Expression Regulation Ligands Oligodendroglia Peripheral Nervous System RNA Messenger Rats Stem Cell Niche Stem Cells Wnt Signaling Pathway Wounds and Injuries
Enhancing Mitofusin/Marf ameliorates neuromuscular dysfunction in Drosophila models of TDP-43 proteinopathies.
Bilal Khalil, Marie-Jeanne Cabirol-Pol, Laetitia Miguel, Alexander J Whitworth, Magalie Lecourtois, Jean-Charles Liévens
Apr 05, 2019
ALS Dynamin-related protein 1 FTLD Mitochondria Mitochondrial Dynamics Animals DNA-Binding Proteins Disease Models Animal Drosophila Drosophila Proteins Dynamins Gene Expression Locomotion Membrane Proteins Mitochondria Mitochondrial Dynamics Molecular Targeted Therapy Neuromuscular Junction Neurons RNA Messenger TDP-43 Proteinopathies
Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment.
Alexandra Bezler, Fabian Braukmann, Sean M West, Arthur Duplan, Raffaella Conconi, Frédéric Schütz, Pierre Gönczy, Fabio Piano, Kristin Gunsalus, Eric A Miska, Laurent Keller
Feb 15, 2019
TransRate
TransRate is a tool for reference-free quality assessment of de novo transcriptome assemblies. Using only the sequenced reads and the assembly as input, we show that multiple common artifacts of de novo transcriptome assembly...
The pathogenesis of mesothelioma is driven by a dysregulated translatome.
Stefano Grosso, Alberto Marini, Katarina Gyuraszova, Johan Vande Voorde, Aristeidis Sfakianos, Gavin D Garland, Angela Rubio Tenor, Ryan Mordue, Tanya Chernova, Nobu Morone, Marco Sereno, Claire P Smith, Leah Officer, Pooyeh Farahmand, Claire Rooney, David Sumpton, Madhumita Das, Ana Teodósio, Catherine Ficken, Maria Guerra Martin, Ruth V Spriggs, Xiao-Ming Sun, Martin Bushell, Owen J Sansom, Daniel Murphy, Marion MacFarlane, John P C Le Quesne, Anne E Willis
Sep 14, 2021
Tumor Cells Cultured Mitochondria Polyribosomes Animals Mice Inbred C57BL Mice Knockout Humans asbestos Naphthyridines RNA Messenger Protein Biosynthesis Oncogenes Mechanistic Target of Rapamycin Complex 1 Mechanistic Target of Rapamycin Complex 2 Mesothelioma Malignant
Published by: Nature Communications
Tubular epithelial cells in renal clear cell carcinoma express high RIPK1/3 and show increased susceptibility to TNF receptor 1-induced necroptosis.
Acrylamides Apoptosis Carcinoma Renal Cell Epithelial Cells Gene Expression Regulation Neoplastic Humans In Situ Nick-End Labeling Kidney Neoplasms Kidney Tubules Necrosis Organ Culture Techniques Quinazolinones RNA Messenger Receptor-Interacting Protein Serine-Threonine Kinases Receptors Tumor Necrosis Factor Type I Signal Transduction Sulfonamides Tumor Necrosis Factor-alpha Up-Regulation
Extent, causes, and consequences of small RNA expression variation in human adipose tissue.
Leopold Parts, Åsa K Hedman, Sarah Keildson, Andrew J Knights, Cei Abreu-Goodger, Martijn van de Bunt, José Afonso Guerra-Assunção, Nenad Bartonicek, Stijn van Dongen, Reedik Mägi, James Nisbet, Amy Barrett, Mattias Rantalainen, Alexandra C Nica, Michael A Quail, Kerrin S Small, Daniel Glass, Anton J Enright, John Winn, MuTHER Consortium, Panos Deloukas, Emmanouil T Dermitzakis, Mark I McCarthy, Timothy D Spector, Richard Durbin, Cecilia M Lindgren
Jan 18, 2018
Small RNAs are functional molecules that modulate mRNA transcripts and have been implicated in the aetiology of several common diseases. However, little is known about the extent of their variability within the human population....
Animals Blood Glucose Body Fat Distribution Body Mass Index Fasting Female Gene Expression Regulation genetic variation Genome-Wide Association Study Genotype High-Throughput Nucleotide Sequencing Humans Insulin MicroRNAs Middle Aged Polymorphism Single Nucleotide RNA Messenger RNA Small Nucleolar RNA Small Untranslated Subcutaneous Fat
Analysis of genetically determined gene expression suggests role of inflammatory processes in exfoliation syndrome.
Jibril B Hirbo, Francesca Pasutto, Eric R Gamazon, Patrick Evans, Priyanka Pawar, Daniel Berner, Julia Sealock, Ran Tao, Peter S Straub, Anuar I Konkashbaev, Max A Breyer, Ursula Schlötzer-Schrehardt, André Reis, Milam A Brantley, Chiea C Khor, Karen M Joos, Nancy J Cox
Mar 19, 2023
Background
Exfoliation syndrome (XFS) is an age-related systemic disorder characterized by excessive production and progressive accumulation of abnormal extracellular material, with pathognomonic ocular manifestations....
GWAS transcriptomics Exfoliation syndrome TWAS GTEx predicted expressions Humans Amino Acid Oxidoreductases DNA-Binding Proteins RNA Messenger Gene Expression Mutation Missense Polymorphism Single Nucleotide B7 Antigens
Published by: BMC genomics
Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.
Max Koppers, Roberta Cagnetta, Toshiaki Shigeoka, Lucia Cs Wunderlich, Pedro Vallejo-Ramirez, Julie Qiaojin Lin, Sixian Zhao, Maximilian AH Jakobs, Asha Dwivedy, Michael S Minett, Anaïs Bellon, Clemens F Kaminski, William A Harris, John G Flanagan, Christine E Holt
Nov 25, 2019
Extrinsic cues trigger the local translation of specific mRNAs in growing axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is...
RNA-binding protein axon developmental biology guidance receptor human local protein synthesis mRNA neuroscience Retinal ganglion cell Xenopus Animals Axons Protein Biosynthesis Proteome RNA Messenger RNA-Binding Proteins Receptors Cell Surface Retinal Ganglion Cells Ribosomes Signal Transduction Xenopus laevis
Interferon-β-induced miR-1 alleviates toxic protein accumulation by controlling autophagy.
Camilla Nehammer, Patrick Ejlerskov, Sandeep Gopal, Ava Handley, Leelee Ng, Pedro Moreira, Huikyong Lee, Shohreh Issazadeh-Navikas, David C Rubinsztein, Roger Pocock
Dec 10, 2019
Appropriate regulation of autophagy is crucial for clearing toxic proteins from cells. Defective autophagy results in accumulation of toxic protein aggregates that detrimentally affect cellular function and organismal survival....
C. elegans Autophagy Biochemistry chemical biology Genetics Genomics human cells mammalian cells microRNA protein aggregation 3' Untranslated Regions Animals Autophagy Base Sequence Caenorhabditis elegans Caenorhabditis elegans Proteins GTPase-Activating Proteins HeLa Cells Humans Huntingtin Protein Interferon-beta Mice MicroRNAs Mutant Proteins Peptides Protein Aggregates RNA Messenger rab GTP-Binding Proteins rab7 GTP-Binding Proteins
The mTOR regulated RNA-binding protein LARP1 requires PABPC1 for guided mRNA interaction.
Ewan M Smith, Nour El Houda Benbahouche, Katherine Morris, Ania Wilczynska, Sarah Gillen, Tobias Schmidt, Hedda A Meijer, Rebekah Jukes-Jones, Kelvin Cain, Carolyn Jones, Mark Stoneley, Joseph A Waldron, Cameron Bell, Bruno D Fonseca, Sarah Blagden, Anne E Willis, Martin Bushell
Feb 26, 2021
The mammalian target of rapamycin (mTOR) is a critical regulator of cell growth, integrating multiple signalling cues and pathways. Key among the downstream activities of mTOR is the control of the protein synthesis machinery....
5' Untranslated Regions Autoantigens Gene Expression Regulation Genes Reporter HeLa Cells Humans Mechanistic Target of Rapamycin Complex 1 Mechanistic Target of Rapamycin Complex 2 Mutagenesis Site-Directed Mutation Missense Naphthyridines Point Mutation Poly(A)-Binding Protein I Polyribosomes Protein Biosynthesis RNA Interference RNA Messenger RNA-Binding Proteins Recombinant Fusion Proteins Ribonucleoproteins TOR Serine-Threonine Kinases
Embryonic origin and serial homology of gill arches and paired fins in the skate, Leucoraja erinacea.
Paired fins are a defining feature of the jawed vertebrate body plan, but their evolutionary origin remains unresolved. Gegenbaur proposed that paired fins evolved as gill arch serial homologues, but this hypothesis is now...
Ribosome collisions trigger cis-acting feedback inhibition of translation initiation.
Szymon Juszkiewicz, Greg Slodkowicz, Zhewang Lin, Paula Freire-Pritchett, Sew-Yeu Peak-Chew, Ramanujan S Hegde
Jul 25, 2020
Translation of aberrant mRNAs can cause ribosomes to stall, leading to collisions with trailing ribosomes. Collided ribosomes are specifically recognised by ZNF598 to initiate protein and mRNA quality control pathways. Here we...
The HIF-1/glial TIM-3 axis controls inflammation-associated brain damage under hypoxia.
Han Seok Koh, Chi Young Chang, Sae-Bom Jeon, Hee Jung Yoon, Ye-Hyeon Ahn, Hyung-Seok Kim, In-Hoo Kim, Sung Ho Jeon, Randall S Johnson, Eun Jung Park
Mar 13, 2020
Inflammation is closely related to the extent of damage following cerebral ischaemia, and the targeting of this inflammation has emerged as a promising therapeutic strategy. Here, we present that hypoxia-induced glial T-cell...
Animals Astrocytes Brain Carotid Artery Common Cell Movement Cells Cultured Hepatitis A Virus Cellular Receptor 2 Hypoxia-Inducible Factor 1 alpha Subunit Hypoxia-Ischemia Brain Immunohistochemistry In Vitro Techniques Inflammation Ligation Magnetic Resonance Imaging Mesencephalon Mice Microglia Neuroglia Neutrophils RNA Messenger Receptors Virus Reverse Transcriptase Polymerase Chain Reaction
FGF8 isoform b expression in human prostate cancer.
Overexpression of fibroblast growth factor 8 (FGF8) mRNA has been previously described in prostate cancer. Of its four isoforms, FGF8b is thought to be the most important in carcinogenesis. We hypothesised that immunodetection...
Suppression of Aβ toxicity by puromycin-sensitive aminopeptidase is independent of its proteolytic activity.
Antonina J Kruppa, Stanislav Ott, Dhia S Chandraratna, James A Irving, Richard M Page, Elena Speretta, Tiffany Seto, Luiz Miguel Camargo, Stefan J Marciniak, David A Lomas, Damian C Crowther
May 18, 2018
The accumulation of β-amyloid (Aβ) peptide in the brain is one of the pathological hallmarks of Alzheimer's disease and is thought to be of primary aetiological significance. In an unbiased genetic screen, we identified...
Alzheimer Amyloid Autophagy Proteolysis Puromycin-sensitive aminopeptidase Alzheimer Disease Aminopeptidases Amyloid beta-Peptides Animals Animals Genetically Modified Autophagy Blotting Western Brain Drosophila melanogaster Enzyme-Linked Immunosorbent Assay Flow Cytometry Fluorescent Antibody Technique Humans Immunoenzyme Techniques Neuroblastoma Neurons Proteolysis puromycin RNA Messenger Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Tumor Cells Cultured
Developmentally regulated instability of the GPI-PLC mRNA is dependent on a short-lived protein factor.
The expression of the vast majority of protein coding genes in trypanosomes is regulated exclusively at the post-transcriptional level. Developmentally regulated mRNAs that vary in levels of expression have provided an insight...
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