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Allostery and dynamics in small G proteins.
The Ras family of small guanine nucleotide-binding proteins behave as molecular switches: they are switched off and inactive when bound to GDP but can be activated by GTP binding in response to signal transduction pathways....
Hepatitis C cross-genotype immunity and implications for vaccine development.
Nazrul Islam, Mel Krajden, Jean Shoveller, Paul Gustafson, Mark Gilbert, Jason Wong, Mark W Tyndall, Naveed Zafar Janjua, The BC-HTC Team
Sep 05, 2018
While about a quarter of individuals clear their primary hepatitis C (HCV) infections spontaneously, clearance (spontaneous or treatment-induced) does not confer sterilizing immunity against a future infection. Since successful...
How release of phosphate from mammalian F1-ATPase generates a rotary substep.
The rotation of the central stalk of F1-ATPase is driven by energy derived from the sequential binding of an ATP molecule to its three catalytic sites and the release of the products of hydrolysis. In human F1-ATPase, each 360°...
ATP synthase Mitochondria phosphate release rotary substep Adenosine Diphosphate Animals Catalytic Domain Cattle Crystallography X-Ray Humans Hydrolysis Mitochondria Models Molecular Molecular Motor Proteins Phosphates Protein Binding Protein Structure Secondary Proton-Translocating ATPases Temperature
Relocation of active site carboxylates in major facilitator superfamily multidrug transporter LmrP reveals plasticity in proton interactions
Recombinant amyloid beta-peptide production by coexpression with an affibody ligand.
Bertil Macao, Wolfgang Hoyer, Anders Sandberg, Ann-Christin Brorsson, Christopher M Dobson, Torleif Härd
Jun 14, 2011
Structure and mechanism of a bacterial host-protein citrullinating virulence factor, Porphyromonas gingivalis peptidylarginine deiminase.
Theodoros Goulas, Danuta Mizgalska, Irene Garcia-Ferrer, Tomasz Kantyka, Tibisay Guevara, Borys Szmigielski, Aneta Sroka, Claudia Millán, Isabel Usón, Florian Veillard, Barbara Potempa, Piotr Mydel, Maria Solà, Jan Potempa, F Xavier Gomis-Rüth
Oct 19, 2020
Citrullination is a post-translational modification of higher organisms that deiminates arginines in proteins and peptides. It occurs in physiological processes but also pathologies such as multiple sclerosis, fibrosis...
Co-repressor, co-activator and general transcription factor
At face value, the Sin3 histone deacetylase (HDAC) complex appears to be a prototypical co-repressor complex, that is, a multi-protein complex recruited to chromatin by DNA bound repressor proteins to facilitate local histone...
Prime-boost vaccination of mice and rhesus macaques with two novel adenovirus vectored COVID-19 vaccine candidates.
Shengxue Luo, Panli Zhang, Bochao Liu, Chan Yang, Chaolan Liang, Qi Wang, Ling Zhang, Xi Tang, Jinfeng Li, Shuiping Hou, Jinfeng Zeng, Yongshui Fu, Jean-Pierre Allain, Tingting Li, Yuming Zhang, Chengyao Li
Jun 17, 2021
ABSTRACTCOVID-19 vaccines are being developed urgently worldwide. Here, we constructed two adenovirus vectored COVID-19 vaccine candidates of Sad23L-nCoV-S and Ad49L-nCoV-S carrying the full-length gene of SARS-CoV-2 spike...
COVID-19 Vaccines human adenovirus 49 vector mice and non-human primates prime-boost vaccination simian adenovirus 23 vector Adenoviridae Animals Antibodies Neutralizing Antibodies Viral covid-19 COVID-19 Vaccines Female Genetic Vectors HEK293 Cells Humans Immunization Secondary Macaca mulatta Male Mice Mice Inbred BALB C Mice Inbred C57BL SARS-CoV-2 T-Lymphocytes
Computational design of transmembrane pores.
Chunfu Xu, Peilong Lu, Tamer M Gamal El-Din, Xue Y Pei, Matthew C Johnson, Atsuko Uyeda, Matthew J Bick, Qi Xu, Daohua Jiang, Hua Bai, Gabriella Reggiano, Yang Hsia, TJ Brunette, Jiayi Dou, Dan Ma, Eric M Lynch, Scott E Boyken, Po-Ssu Huang, Lance Stewart, Frank Dimaio, Justin M Kollman, Ben F Luisi, Tomoaki Matsuura, William A Catterall, David Baker
May 17, 2021
Transmembrane channels and pores have key roles in fundamental biological processes1 and in biotechnological applications such as DNA nanopore sequencing2-4, resulting in considerable interest in the design of pore-containing...
Cell Line Computer Simulation Cryoelectron Microscopy Crystallography X-Ray Electric Conductivity Escherichia coli Genes Synthetic Hydrazines Ion Channels Ion Transport Liposomes Models Molecular Patch-Clamp Techniques Porins Protein Engineering Protein Structure Secondary Solubility synthetic biology water
Clinico-Genetic, Imaging and Molecular Delineation of COQ8A-Ataxia
Andreas Traschütz, Tommaso Schirinzi, Lucia Laugwitz, Nathan H Murray, Craig A Bingman, Selina Reich, Jan Kern, Anna Heinzmann, Gessica Vasco, Enrico Bertini, Ginevra Zanni, Alexandra Durr, Stefania Magri, Franco Taroni, Alessandro Malandrini, Jonathan Baets, Peter De Jonghe, Willem de Ridder, Matthieu Bereau, Stephanie Demuth, Christos Ganos, A Nazli Basak, Hasmet Hanagasi, Semra Hiz Kurul, Benjamin Bender, Ludger Schöls, Ute Grasshoff, Thomas Klopstock, Rita Horvath, Bart van de Warrenburg, Lydie Burglen, Christelle Rougeot, Claire Ewenczyk, Michel Koenig, Filippo M Santorelli, Mathieu Anheim, Renato P Munhoz, Tobias Haack, Felix Distelmaier, David J Pagliarini, Hélène Puccio, Matthis Synofzik
May 18, 2020
OBJECTIVE: To foster trial-readiness of coenzyme Q8A (COQ8A)-ataxia, we map the clinicogenetic, molecular, and neuroimaging spectrum of COQ8A-ataxia in a large worldwide cohort, and provide first progression data, including...
The affinity purification and characterization of ATP synthase complexes from mitochondria.
Michael J Runswick, John V Bason, Martin G Montgomery, Graham C Robinson, Ian M Fearnley, John E Walker
Aug 11, 2017
The mitochondrial F₁-ATPase inhibitor protein, IF₁, inhibits the hydrolytic, but not the synthetic activity of the F-ATP synthase, and requires the hydrolysis of ATP to form the inhibited complex. In this complex, the α-helical...
Phenylalanine in the pore of the Erwinia ligand-gated ion channel modulates picrotoxinin potency but not receptor function.
Amino Acid Sequence Animals Bacterial Proteins Binding Sites Binding Competitive Cysteine Loop Ligand-Gated Ion Channel Receptors Erwinia Female GABA-A Receptor Antagonists Ion Channel Gating Membrane Potentials Models Molecular Molecular Sequence Data Mutation Missense Oocytes Phenylalanine Picrotoxin Protein Structure Secondary Protein Structure Tertiary Sequence Homology Amino Acid Sesterterpenes Xenopus laevis gamma-Aminobutyric Acid
Emily R Rowe, Michael L Mimmack, Antonio D Barbosa, Afreen Haider, Iona Isaac, Myriam M Ouberai, Abdou Rachid Thiam, Satish Patel, Vladimir Saudek, Symeon Siniossoglou, David B Savage
Jan 13, 2016
11-mer repeat amphipathic helix lipid droplet lipodystrophy Lipolysis membrane membrane targeting monolayer perilipin phospholipid Amino Acid Sequence Animals Binding Sites Biological Transport COS Cells Carrier Proteins Chlorocebus aethiops Gene Expression Humans Hydrophobic and Hydrophilic Interactions Lipid Droplets Membrane Proteins Micelles Models Molecular Molecular Sequence Data Mutation Perilipin-1 Perilipin-2 Perilipin-3 Phosphoproteins Protein Binding Protein Interaction Domains and Motifs Protein Structure Secondary Protein Transport Recombinant Proteins Saccharomyces cerevisiae Sequence Alignment Transgenes Vesicular Transport Proteins
GADIS
Tyler S Harmon, Michael D Crabtree, Sarah L Shammas, Ammon E Posey, Jane Clarke, Rohit V Pappu
Aug 19, 2016
Outer membrane β-barrel protein folding is physically controlled by periplasmic lipid head groups and BamA.
Dennis Gessmann, Yong Hee Chung, Emily J Danoff, Ashlee M Plummer, Clifford W Sandlin, Nathan R Zaccai, Karen G Fleming
Jul 04, 2016
beta-barrel transmembrane protein membrane protein folding Bacterial Outer Membrane Proteins Biocatalysis Cell Membrane Escherichia coli Escherichia coli Proteins Kinetics Lipids Models Biological Periplasm Phenylalanine Phosphatidylethanolamines Phosphatidylglycerols Protein Folding Protein Structure Secondary
Modular type I polyketide synthase acyl carrier protein domains share a common N-terminally extended fold.
Acyl carrier protein (ACP) domains act as interaction hubs within modular polyketide synthase (PKS) systems, employing specific protein-protein interactions to present acyl substrates to a series of enzyme active sites. Many...
Characterising the association of latency with α(1)-antitrypsin polymerisation using a novel monoclonal antibody.
Lu Tan, Juan Perez, Marianna Mela, Elena Miranda, Keith A Burling, Farshid N Rouhani, Dawn L DeMeo, Imran Haq, James A Irving, Adriana Ordóñez, Jennifer A Dickens, Mark Brantly, Stefan J Marciniak, Graeme JM Alexander, Bibek Gooptu, David A Lomas
May 18, 2018
α1-Antitrypsin is primarily synthesised in the liver, circulates to the lung and protects pulmonary tissues from proteolytic damage. The Z mutant (Glu342Lys) undergoes inactivating conformational change and polymerises. Polymers...
Application of Lysine-specific Labeling to Detect Transient Interactions Present During Human Lysozyme Amyloid Fibril Formation.
Minkoo Ahn, Christopher A Waudby, Ana Bernardo-Gancedo, Erwin De Genst, Anne Dhulesia, Xavier Salvatella, John Christodoulou, Christopher M Dobson, Janet R Kumita
Jun 04, 2018
Populating transient and partially unfolded species is a crucial step in the formation and accumulation of amyloid fibrils formed from pathogenic variants of human lysozyme linked with a rare but fatal hereditary systemic...
Structural plasticity of D3-D14 ubiquitin ligase in strigolactone signalling.
Nitzan Shabek, Fabrizio Ticchiarelli, Haibin Mao, Thomas R Hinds, Ottoline Leyser, Ning Zheng
Dec 11, 2018
Analysis of Differential Efficacy and Affinity of GABAA (α1/α2) Selective Modulators.
Qurrat U Ain, Robert M Owen, Kiyoyuki Omoto, Rubben Torella, Krishna C Bulusu, David C Pryde, Robert C Glen, Julian E Fuchs, Andreas Bender
Nov 26, 2018
Selective modulators of the γ-amino butyric acid (GABAA) family of receptors have the potential to treat a range of disease states related to cognition, pain, and anxiety. While the development of various α subunit-selective...
GABAA α1 GABAA α2 affinity modeling Benzodiazepines proteochemometric modeling relative efficacy selective modulators Amino Acid Sequence Animals Benzodiazepines Butyric Acid GABA-A Receptor Agonists Humans Hydrogen Bonding Molecular Dynamics Simulation Molecular Sequence Data principal component analysis Protein Structure Secondary Receptors GABA
Varenicline Interactions at the 5-HT3 Receptor Ligand Binding Site are Revealed by 5-HTBP.
Cys-loop receptor Ligand-gated ion channel serotonin receptor Amino Acid Sequence Animals Binding Sites Carrier Proteins Crystallography X-Ray HEK293 Cells Humans Hydrogen Bonding Mice Models Molecular Mutation Oocytes Patch-Clamp Techniques Protein Structure Secondary Receptors Serotonin 5-HT3 Serotonin Serotonin Agents Varenicline water Xenopus
Structure of human saposin A at lysosomal pH.
Structure of the periplasmic adaptor protein from a major facilitator superfamily (MFS) multidrug efflux pump.
Philip Hinchliffe, Nicholas P Greene, Neil G Paterson, Allister Crow, Colin Hughes, Vassilis Koronakis
Jul 11, 2014
Adaptor protein antibiotic resistance Crystal structure Major facilitator superfamily Multidrug efflux Amino Acid Sequence Aquifoliaceae Bacterial Proteins Conserved Sequence Crystallography X-Ray Ligands Membrane Transport Proteins Models Molecular Molecular Sequence Data Protein Structure Secondary Protein Structure Tertiary
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