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Inhibition of the RNA polymerase III-mediated dsDNA-sensing pathway of innate immunity by vaccinia virus protein E3.
The vaccinia virus E3 protein is an important intracellular modulator of innate immunity that can be split into distinct halves. The C terminus contains a well defined dsRNA-binding domain, whereas the N terminus contains a...
Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research.
Takuya Kobayashi, Nobuhiro Morone, Taku Kashiyama, Hideto Oyamada, Nagomi Kurebayashi, Takashi Murayama
May 28, 2019
BACKGROUND: Genetically encoded tag is a powerful tool for protein research. Various kinds of tags have been developed: fluorescent proteins for live-cell imaging, affinity tags for protein isolation, and epitope tags for...
Structural insights into the mechanism of negative regulation of single-box high mobility group proteins by the acidic tail domain.
Katherine Stott, Matthew Watson, Mark J Bostock, Simon A Mortensen, Andrew Travers, Klaus D Grasser, Jean O Thomas
Apr 13, 2015
Acidic Regulatory Domain Casein Kinase 2 (CK2) Chromatin HMGB Protein Intrinsically Disordered Protein Nuclear Magnetic Resonance (NMR) Paramagnetic Relaxation Enhancement Phosphorylation Protein Kinase C Protein Kinase C (PKC) Animals Drosophila Proteins Drosophila melanogaster High Mobility Group Proteins Magnetic Resonance Spectroscopy Models Molecular Mutant Proteins Phosphorylation Plant Proteins Protein Structure Tertiary Structure-Activity Relationship Zea mays
Characterization of spindle checkpoint kinase Mps1 reveals domain with functional and structural similarities to tetratricopeptide repeat motifs of Bub1 and BubR1 checkpoint kinases.
Semin Lee, Philippe Thebault, Luca Freschi, Sylvie Beaufils, Tom L Blundell, Christian R Landry, Victor M Bolanos-Garcia, Sabine Elowe
Mar 12, 2021
Kinetochore targeting of the mitotic kinases Bub1, BubR1, and Mps1 has been implicated in efficient execution of their functions in the spindle checkpoint, the self-monitoring system of the eukaryotic cell cycle that ensures...
Amino Acid Motifs Animals Cattle Cell Cycle Proteins Chromosomes Human Computational Biology Enzyme Stability evolution Molecular HeLa Cells Humans Hydrogen-Ion Concentration M Phase Cell Cycle Checkpoints Mice Models Molecular Protein Multimerization Protein Serine-Threonine Kinases Protein Structure Tertiary Protein Transport Protein-Tyrosine Kinases Rats
Dynamic condensation of linker histone C-terminal domain regulates chromatin structure.
The basic and intrinsically disordered C-terminal domain (CTD) of the linker histone (LH) is essential for chromatin compaction. However, its conformation upon nucleosome binding and its impact on chromatin organization remain...
TOPBP1 recruits TOP2A to ultra-fine anaphase bridges to aid in their resolution.
Ronan Broderick, Jadwiga Nieminuszczy, Andrew N Blackford, Alicja Winczura, Wojciech Niedzwiedz
May 22, 2015
Anaphase Antigens Neoplasm Carrier Proteins Cell Cycle Proteins Cell Line Tumor Centromere Chromatids Chromosomes DNA DNA Topoisomerases Type II DNA-Binding Proteins Gene Expression Regulation Gene Expression Regulation Neoplastic Green Fluorescent Proteins HEK293 Cells HeLa Cells Humans Microscopy Fluorescence Mitosis Mutation Nuclear Proteins Poly-ADP-Ribose Binding Proteins Protein Binding Protein Structure Tertiary
Inhibition of apoptosis and NF-κB activation by vaccinia protein N1 occur via distinct binding surfaces and make different contributions to virulence.
Carlos Maluquer de Motes, Samantha Cooray, Hongwei Ren, Gabriel MF Almeida, Kieran McGourty, Mohammad W Bahar, David I Stuart, Jonathan M Grimes, Stephen C Graham, Geoffrey L Smith
Aug 08, 2017
Vaccinia virus (VACV) protein N1 is an intracellular virulence factor and belongs to a family of VACV B-cell lymphoma (Bcl)-2-like proteins whose members inhibit apoptosis or activation of pro-inflammatory transcription factors...
AtEAF1 is a potential platform protein for Arabidopsis NuA4 acetyltransferase complex.
Tomasz Bieluszewski, Lukasz Galganski, Weronika Sura, Anna Bieluszewska, Mateusz Abram, Agnieszka Ludwikow, Piotr Andrzej Ziolkowski, Jan Sadowski
Sep 29, 2016
BACKGROUND: Histone acetyltransferase complex NuA4 and histone variant exchanging complex SWR1 are two chromatin modifying complexes which act cooperatively in yeast and share some intriguing structural similarities. Protein...
Acetylation Acetyltransferases Amino Acid Sequence Arabidopsis Arabidopsis Proteins Cell Nucleus Flowers Gene Expression Regulation Plant Genes Plant Histones Hydroxamic Acids MicroRNAs Molecular Sequence Data Multiprotein Complexes Mutation Protein Binding Protein Structure Tertiary RNA Messenger Saccharomyces cerevisiae Transcription Genetic
Peptide aptamers as new tools to modulate clathrin-mediated internalisation--inhibition of MT1-MMP internalisation.
Predicted mouse peroxisome-targeted proteins and their actual subcellular locations.
Phenylalanine in the pore of the Erwinia ligand-gated ion channel modulates picrotoxinin potency but not receptor function.
Amino Acid Sequence Animals Bacterial Proteins Binding Sites Binding Competitive Cysteine Loop Ligand-Gated Ion Channel Receptors Erwinia Female GABA-A Receptor Antagonists Ion Channel Gating Membrane Potentials Models Molecular Molecular Sequence Data Mutation Missense Oocytes Phenylalanine Picrotoxin Protein Structure Secondary Protein Structure Tertiary Sequence Homology Amino Acid Sesterterpenes Xenopus laevis gamma-Aminobutyric Acid
Response and resistance to BET bromodomain inhibitors in triple-negative breast cancer.
Shaokun Shu, Charles Y Lin, Housheng Hansen He, Robert M Witwicki, Doris P Tabassum, Justin M Roberts, Michalina Janiszewska, Sung Jin Huh, Yi Liang, Jeremy Ryan, Ernest Doherty, Hisham Mohammed, Hao Guo, Daniel G Stover, Muhammad B Ekram, Jonathan Brown, Clive D'Santos, Ian E Krop, Deborah Dillon, Michael McKeown, Christopher Ott, Jun Qi, Min Ni, Prakash K Rao, Melissa Duarte, Shwu-Yuan Wu, Cheng-Ming Chiang, Lars Anders, Richard A Young, Eric Winer, Antony Letai, William T Barry, Jason S Carroll, Henry Long, Myles Brown, X Shirley Liu, Clifford A Meyer, James E Bradner, Kornelia Polyak
May 02, 2019
Triple-negative breast cancer (TNBC) is a heterogeneous and clinically aggressive disease for which there is no targeted therapy. BET bromodomain inhibitors, which have shown efficacy in several models of cancer, have not been...
Animals Azepines Binding Competitive Casein Kinase II Cell Cycle Proteins Cell Line Tumor Cell Proliferation Chromatin Drug Resistance Neoplasm Epigenesis Genetic Female Gene Expression Regulation Neoplastic Genome Human Humans Mediator Complex Subunit 1 Mice Nuclear Proteins Phosphorylation Phosphoserine Protein Binding Protein Phosphatase 2 Protein Structure Tertiary Proteomics Transcription Factors Transcription Genetic Triazoles Triple Negative Breast Neoplasms Xenograft Model Antitumor Assays
Modular type I polyketide synthase acyl carrier protein domains share a common N-terminally extended fold.
Acyl carrier protein (ACP) domains act as interaction hubs within modular polyketide synthase (PKS) systems, employing specific protein-protein interactions to present acyl substrates to a series of enzyme active sites. Many...
Identification of a lysosomal pathway regulating degradation of the bone morphogenetic protein receptor type II.
Hannah J Durrington, Paul D Upton, Simon Hoer, Jessica Boname, Benjamin J Dunmore, Jun Yang, Trina K Crilley, Lynn M Butler, David J Blackbourn, Gerard B Nash, Paul J Lehner, Nicholas W Morrell
Aug 28, 2020
Bone morphogenetic proteins (BMPs) are critically involved in early development and cell differentiation. In humans, dysfunction of the bone morphogenetic protein type II receptor (BMPR-II) is associated with pulmonary arterial...
Germ-line DICER1 mutations do not make a major contribution to the etiology of familial testicular germ cell tumours.
Nelly Sabbaghian, Amin Bahubeshi, Andrew Y Shuen, Peter A Kanetsky, Marc D Tischkowitz, Katherine L Nathanson, William D Foulkes
May 02, 2013
Small-molecule inhibitors that target protein-protein interactions in the RAD51 family of recombinases.
Duncan E Scott, Anthony G Coyne, Ashok Venkitaraman, Tom L Blundell, Chris Abell, Marko Hyvönen
Dec 16, 2014
BRCA2 RAD51 biophysics homologous recombination INHIBITORS protein-protein interactions Amino Acid Motifs BRCA2 Protein Binding Sites Drug Design Humans Molecular Dynamics Simulation Protein Binding Protein Structure Tertiary Pyrococcus furiosus Rad51 Recombinase Small Molecule Libraries Structure-Activity Relationship Thermodynamics
Structure of the periplasmic adaptor protein from a major facilitator superfamily (MFS) multidrug efflux pump.
Philip Hinchliffe, Nicholas P Greene, Neil G Paterson, Allister Crow, Colin Hughes, Vassilis Koronakis
Jul 11, 2014
Adaptor protein antibiotic resistance Crystal structure Major facilitator superfamily Multidrug efflux Amino Acid Sequence Aquifoliaceae Bacterial Proteins Conserved Sequence Crystallography X-Ray Ligands Membrane Transport Proteins Models Molecular Molecular Sequence Data Protein Structure Secondary Protein Structure Tertiary
Structure of an atypical periplasmic adaptor from a multidrug efflux pump of the spirochete Borrelia burgdorferi.
Nicholas P Greene, Philip Hinchliffe, Allister Crow, Abdessamad Ababou, Colin Hughes, Vassilis Koronakis
Sep 19, 2014
Adaptor protein antibiotic resistance Crystal structure HME IM MD MDR MP Multidrug efflux OM RMSDs heavy metal efflux inner membrane membrane proximal Molecular dynamics multidrug resistance outer membrane root mean square deviations Bacterial Outer Membrane Proteins Borrelia burgdorferi Crystallography X-Ray Escherichia coli Gene Expression Molecular Dynamics Simulation Multidrug Resistance-Associated Proteins Periplasm Protein Structure Secondary Protein Structure Tertiary Recombinant Proteins Selenomethionine
Structure of human saposin A at lysosomal pH.
Structure-function analysis of the Bacillus megaterium GerUD spore germinant receptor protein.
Characterisation of denatured states of sensory rhodopsin II by solution-state NMR.
Sensory rhodopsin II (pSRII), a retinal-binding photophobic receptor from Natronomonas pharaonis, is a novel model system for membrane protein folding studies. Recently, the SDS-denatured states and the kinetics for reversible...
Urban Endocrine Disruptors Targeting Breast Cancer Proteins.
Benzhydryl Compounds Binding Sites Breast Neoplasms Catalase Cytochrome P-450 CYP1A2 Databases Chemical Databases Protein Dioxins Endocrine Disruptors Environmental Pollutants Female Humans Molecular Dynamics Simulation Phenols Protein Binding Protein Structure Tertiary Recombinant Proteins Sex Hormone-Binding Globulin
Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion.
Guido Papa, Donna L Mallery, Anna Albecka, Lawrence G Welch, Jérôme Cattin-Ortolá, Jakub Luptak, David Paul, Harvey T McMahon, Ian G Goodfellow, Andrew Carter, Sean Munro, Leo C James
Mar 05, 2021
Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) infects cells by binding to the host cell receptor ACE2 and undergoing virus-host membrane fusion. Fusion is triggered by the protease TMPRSS2, which processes the...
The Role of ICL1 and H8 in Class B1 GPCRs; Implications for Receptor Activation.
Ian Winfield, Kerry Barkan, Sarah Routledge, Nathan J Robertson, Matthew Harris, Ali Jazayeri, John Simms, Christopher A Reynolds, David R Poyner, Graham Ladds
Jan 28, 2022
The first intracellular loop (ICL1) of G protein-coupled receptors (GPCRs) has received little attention, although there is evidence that, with the 8th helix (H8), it is involved in early conformational changes following...
CLR GPCRs RAMPs Mutagenesis signaling bias Amino Acid Motifs Calcitonin Receptor-Like Protein Calcium Signaling Cyclic AMP HEK293 Cells Humans MAP Kinase Signaling System Molecular Dynamics Simulation Protein Domains Protein Structure Tertiary Receptor Activity-Modifying Protein 1 Receptors Calcitonin Gene-Related Peptide Receptors Corticotropin-Releasing Hormone Receptors G-Protein-Coupled Receptors Glucagon
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