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Results: 48
Loss of skeletal muscle HIF-1alpha results in altered exercise endurance.
Steven D Mason, Richard A Howlett, Matthew J Kim, I Mark Olfert, Michael C Hogan, Wayne McNulty, Reed P Hickey, Peter D Wagner, C Ronald Kahn, Frank J Giordano, Randall S Johnson
Mar 13, 2020
The physiological flux of oxygen is extreme in exercising skeletal muscle. Hypoxia is thus a critical parameter in muscle function, influencing production of ATP, utilization of energy-producing substrates, and manufacture of...
Adenosine Triphosphate Alleles Animals creatine kinase Crosses Genetic Gene Deletion Gene Expression Regulation Genotype Glucose Glycogen Glycolysis Hematocrit Hemoglobins Hypoxia Hypoxia-Inducible Factor 1 alpha Subunit Lactic Acid Male Mice Mice Inbred C57BL Mice Knockout Microscopy Electron Muscle Skeletal Oxygen Physical Conditioning Animal Physical Exertion Polymerase Chain Reaction Promoter Regions Genetic Reverse Transcriptase Polymerase Chain Reaction
The radiosensitising effect of gemcitabine and its main metabolite dFdU under low oxygen conditions is in vitro not dependent on functional HIF-1 protein.
An Wouters, Bea Pauwels, Natalie Burrows, Marc Baay, Vanessa Deschoolmeester, Trung Nghia Vu, Kris Laukens, Paul Meijnders, Dirk Van Gestel, Kaye J Williams, Danielle Van den Weyngaert, Jan B Vermorken, Patrick Pauwels, Marc Peeters, Filip Lardon
May 26, 2021
BACKGROUND: Regions within solid tumours often experience oxygen deprivation, which is associated with resistance to chemotherapy and irradiation. The aim of this study was to evaluate the radiosensitising effect of gemcitabine...
Placental hypoxia, endoplasmic reticulum stress and maternal endothelial sensitisation by sFLT1 in pre-eclampsia.
D Stephen Charnock-Jones
Jul 14, 2015
ER stress Hypoxia pre-eclampsia Unfolded Protein Response sFLT1 Animals Endoplasmic reticulum stress Endothelium Female Humans Hypoxia Hypoxia-Inducible Factor 1 alpha Subunit Membrane Proteins pre-eclampsia Pregnancy Tumor Necrosis Factor-alpha Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-1
Hypoxia-induced nitric oxide production and tumour perfusion is inhibited by pegylated arginine deiminase (ADI-PEG20).
Natalie Burrows, Gaelle Cane, Mathew Robson, Edoardo Gaude, William J Howat, Peter W Szlosarek, R Barbara Pedley, Christian Frezza, Margaret Ashcroft, Patrick H Maxwell
Mar 01, 2016
Animals Arginine Argininosuccinate Synthase Basic Helix-Loop-Helix Transcription Factors Blotting Western Cell Hypoxia Cell Line Tumor HCT116 Cells Humans Hydrolases Hypoxia Hypoxia-Inducible Factor 1 alpha Subunit Mechanistic Target of Rapamycin Complex 1 Mice SCID Multiprotein Complexes Neoplasms Nitric oxide Nitric Oxide Synthase Type II Perfusion Polyethylene Glycols TOR Serine-Threonine Kinases Tumor Burden Unfolded Protein Response Vascular Endothelial Growth Factor A Xenograft Model Antitumor Assays
Hypoxia and tissue destruction in pulmonary TB.
Moerida Belton, Sara Brilha, Roido Manavaki, Francesco Mauri, Kuldip Nijran, Young T Hong, Neva H Patel, Marcin Dembek, Liku Tezera, Justin Green, Rachel Moores, Franklin Aigbirhio, Adil Al-Nahhas, Tim D Fryer, Paul T Elkington, Jon S Friedland
Jun 03, 2016
Tuberculosis Adult Biopsy Cell Hypoxia Cells Cultured Collagenases Epithelial Cells Female Gene Expression Regulation Humans Hypoxia-Inducible Factor 1 alpha Subunit Lung MACROPHAGES Male Matrix Metalloproteinase 1 Microscopy Confocal Middle Aged Mycobacterium tuberculosis Positron Emission Tomography Computed Tomography RNA Messenger Respiratory Mucosa Tuberculosis Pulmonary Up-Regulation Young Adult
Inhibition of NGF deprivation-induced death by low oxygen involves suppression of BIMEL and activation of HIF-1.
Changes in O(2) tension can significantly impact cell survival, yet the mechanisms underlying these effects are not well understood. Here, we report that maintaining sympathetic neurons under low O(2) inhibits apoptosis caused...
Animals Animals Newborn Apoptosis COS Cells Caspases Cell Death Cell Hypoxia Cell Survival Cells Cultured Chlorocebus aethiops Cytochrome c Group DNA-Binding Proteins Fluorescent Antibody Technique Indirect Ganglia Sympathetic Helix-Loop-Helix Motifs Hypoxia-Inducible Factor 1 Hypoxia-Inducible Factor 1 alpha Subunit Immunoblotting JNK Mitogen-Activated Protein Kinases Luciferases Mice Microscopy Confocal Mitochondria Nerve Growth Factor Neurons Nuclear Proteins Rats Rats Sprague-Dawley Superior Cervical Ganglion Transcription Factors
Activation of hypoxia-inducible factor-2 in adipocytes results in pathological cardiac hypertrophy.
Qun Lin, Yan Huang, Carmen J Booth, Volker H Haase, Randall S Johnson, M Celeste Simon, Frank J Giordano, Zhong Yun
Mar 13, 2020
BACKGROUND: Obesity can cause structural and functional abnormalities of the heart via complex but largely undefined mechanisms. Emerging evidence has shown that obesity results in reduced oxygen concentrations, or hypoxia, in...
Adipocytes Cardiomyopathy Hypoxia Inflammation obesity Adipocytes Animals Basic Helix-Loop-Helix Transcription Factors Cardiomegaly Cytokines Disease Models Animal Gene Expression Regulation Genetic Predisposition to Disease Hypoxia-Inducible Factor 1 alpha Subunit Inflammation Mediators Mice Mice Inbred C57BL Mice Knockout Mice Transgenic Myocytes Cardiac Phenotype Signal Transduction Time Factors Von Hippel-Lindau Tumor Suppressor Protein
The HIF-1/glial TIM-3 axis controls inflammation-associated brain damage under hypoxia.
Han Seok Koh, Chi Young Chang, Sae-Bom Jeon, Hee Jung Yoon, Ye-Hyeon Ahn, Hyung-Seok Kim, In-Hoo Kim, Sung Ho Jeon, Randall S Johnson, Eun Jung Park
Mar 13, 2020
Inflammation is closely related to the extent of damage following cerebral ischaemia, and the targeting of this inflammation has emerged as a promising therapeutic strategy. Here, we present that hypoxia-induced glial T-cell...
Animals Astrocytes Brain Carotid Artery Common Cell Movement Cells Cultured Hepatitis A Virus Cellular Receptor 2 Hypoxia-Inducible Factor 1 alpha Subunit Hypoxia-Ischemia Brain Immunohistochemistry In Vitro Techniques Inflammation Ligation Magnetic Resonance Imaging Mesencephalon Mice Microglia Neuroglia Neutrophils RNA Messenger Receptors Virus Reverse Transcriptase Polymerase Chain Reaction
Independence of HIF1a and androgen signaling pathways in prostate cancer.
Maxine GB Tran, Becky AS Bibby, Lingjian Yang, Franklin Lo, Anne Y Warren, Deepa Shukla, Michelle Osborne, James Hadfield, Thomas Carroll, Rory Stark, Helen Scott, Antonio Ramos-Montoya, Charlie Massie, Patrick Maxwell, Catharine ML West, Ian G Mills, David E Neal
Jul 14, 2020
BACKGROUND: Therapeutic targeting of the androgen signaling pathway is a mainstay treatment for prostate cancer. Although initially effective, resistance to androgen targeted therapies develops followed by disease progression to...
Androgen signaling HIF1a signaling Hypoxia prostate cancer Androgen Antagonists Androgens Animals Apoptosis Biomarkers Tumor Cell Proliferation Gene Expression Profiling Gene Expression Regulation Neoplastic Humans Hypoxia Hypoxia-Inducible Factor 1 alpha Subunit Male Mice Prostatic Neoplasms Receptors Androgen Signal Transduction Transcriptional Activation Tumor Cells Cultured Xenograft Model Antitumor Assays
Developmental role of PHD2 in the pathogenesis of pseudohypoxic pheochromocytoma.
Luise Eckardt, Maria Prange-Barczynska, Emma J Hodson, James W Fielding, Xiaotong Cheng, Joanna DCC Lima, Samvid Kurlekar, Gillian Douglas, Peter J Ratcliffe, Tammie Bishop
Nov 22, 2021
Despite a general role for the HIF hydroxylase system in cellular oxygen sensing and tumour hypoxia, cancer-associated mutations of genes in this pathway, including PHD2, PHD1, EPAS1 (encoding HIF-2α) are highly...
Succinate Dehydrogenase Supports Metabolic Repurposing of Mitochondria to Drive Inflammatory Macrophages.
Evanna L Mills, Beth Kelly, Angela Logan, Ana SH Costa, Mukund Varma, Clare E Bryant, Panagiotis Tourlomousis, J Henry M Däbritz, Eyal Gottlieb, Isabel Latorre, Sinéad C Corr, Gavin McManus, Dylan Ryan, Howard T Jacobs, Marten Szibor, Ramnik J Xavier, Thomas Braun, Christian Frezza, Michael P Murphy, Luke A O'Neill
Nov 22, 2017
Activated macrophages undergo metabolic reprogramming, which drives their pro-inflammatory phenotype, but the mechanistic basis for this remains obscure. Here, we demonstrate that upon lipopolysaccharide (LPS) stimulation...
immunometabolism innate immunity macrophage reverse electron transport Succinate Succinate Dehydrogenase Toll-Like Receptors Adenosine Triphosphate Animals Carbonyl Cyanide m-Chlorophenyl Hydrazone Citric Acid Cycle Glycolysis Hypoxia-Inducible Factor 1 alpha Subunit Inflammation Interleukin-10 Lipopolysaccharides Macrophage Activation MACROPHAGES Malonates Membrane potential Mitochondrial Mice Mice Inbred C57BL Mitochondria Mitochondrial Proteins Oxidation-Reduction Oxidative Phosphorylation Oxidoreductases Plant Proteins Reactive Oxygen Species Sequence Analysis RNA Succinate Dehydrogenase Succinic Acid Transcriptome
Mitochondrial Protein Lipoylation and the 2-Oxoglutarate Dehydrogenase Complex Controls HIF1α Stability in Aerobic Conditions.
Stephen P Burr, Ana SH Costa, Guinevere L Grice, Richard T Timms, Ian T Lobb, Peter Freisinger, Roger B Dodd, Gordon Dougan, Paul J Lehner, Christian Frezza, James A Nathan
Nov 21, 2016
Imaging breast cancer using hyperpolarized carbon-13 MRI.
Ferdia A Gallagher, Ramona Woitek, Mary A McLean, Andrew B Gill, Raquel Manzano Garcia, Elena Provenzano, Frank Riemer, Joshua Kaggie, Anita Chhabra, Stephan Ursprung, James T Grist, Charlie J Daniels, Fulvio Zaccagna, Marie-Christine Laurent, Matthew Locke, Sarah Hilborne, Amy Frary, Turid Torheim, Chris Boursnell, Amy Schiller, Ilse Patterson, Rhys Slough, Bruno Carmo, Justine Kane, Heather Biggs, Emma Harrison, Surrin S Deen, Andrew Patterson, Titus Lanz, Zoya Kingsbury, Mark Ross, Bristi Basu, Richard Baird, David J Lomas, Evis Sala, James Wason, Oscar M Rueda, Suet-Feung Chin, Ian B Wilkinson, Martin J Graves, Jean E Abraham, Fiona J Gilbert, Carlos Caldas, Kevin M Brindle
Feb 06, 2020
Hypoxia-induced neutrophil survival is mediated by HIF-1alpha-dependent NF-kappaB activity.
Sarah R Walmsley, Cristin Print, Neda Farahi, Carole Peyssonnaux, Randall S Johnson, Thorsten Cramer, Anastasia Sobolewski, Alison M Condliffe, Andrew S Cowburn, Nicola Johnson, Edwin R Chilvers
Mar 13, 2020
Neutrophils are key effector cells of the innate immune response and are required to migrate and function within adverse microenvironmental conditions. These inflammatory sites are characterized by low levels of oxygen and...
Animals Apoptosis Blotting Western Cell Survival Chemokine CCL4 Cytokines DNA Primers DNA-Binding Proteins Enzyme-Linked Immunosorbent Assay Gliotoxin Humans Hypoxia Hypoxia-Inducible Factor 1 Hypoxia-Inducible Factor 1 alpha Subunit Macrophage Inflammatory Proteins Mice Mice Knockout Mixed Function Oxygenases NF-kappa B p50 Subunit Neutrophils Nuclear Proteins Oligonucleotide Array Sequence Analysis Oligonucleotides Polymerase Chain Reaction Protein Binding Repressor Proteins Sesquiterpenes Transcription Factors
HIF-1α-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity.
Hiroaki Semba, Norihiko Takeda, Takayuki Isagawa, Yuki Sugiura, Kurara Honda, Masaki Wake, Hidenobu Miyazawa, Yoshifumi Yamaguchi, Masayuki Miura, Dana MR Jenkins, Hyunsung Choi, Jung-Whan Kim, Masataka Asagiri, Andrew S Cowburn, Hajime Abe, Katsura Soma, Katsuhiro Koyama, Manami Katoh, Keimon Sayama, Nobuhito Goda, Randall S Johnson, Ichiro Manabe, Ryozo Nagai, Issei Komuro
May 04, 2018
In severely hypoxic condition, HIF-1α-mediated induction of Pdk1 was found to regulate glucose oxidation by preventing the entry of pyruvate into the tricarboxylic cycle. Monocyte-derived macrophages, however, encounter a...
Exploring the molecular interface between hypoxia-inducible factor signalling and mitochondria.
Oxygen is required for the survival of the majority of eukaryotic organisms, as it is important for many cellular processes. Eukaryotic cells utilize oxygen for the production of biochemical energy in the form of adenosine...
CK1δ restrains lipin-1 induction, lipid droplet formation and cell proliferation under hypoxia by reducing HIF-1α/ARNT complex formation.
Maria Kourti, Georgia Ikonomou, Nikolaos-Nikiforos Giakoumakis, Maria Anna Rapsomaniki, Ulf Landegren, Symeon Siniossoglou, Zoi Lygerou, George Simos, Ilias Mylonis
May 14, 2015
CK1δ HIF-1 Hypoxia Lipid Droplets Lipid Metabolism Lipin1 Casein Kinase Idelta Cell Hypoxia Cell Line Cell Proliferation Fluorescence Recovery After Photobleaching HeLa Cells Humans Hypoxia-Inducible Factor 1 alpha Subunit Lipid Droplets Lipid Metabolism Phosphatidate Phosphatase Phosphorylation RNA Interference RNA Small Interfering
Energy status and HIF signalling in chorionic villi show no evidence of hypoxic stress during human early placental development.
T Cindrova-Davies, M Tissot van Patot, L Gardner, E Jauniaux, GJ Burton, DS Charnock-Jones
Dec 03, 2014
HIF-1α energy status first trimester Metabolomics Placenta Adenosine Diphosphate Adenosine Triphosphate Adult Basic Helix-Loop-Helix Transcription Factors Cell Hypoxia Chorionic Villi Energy Metabolism Female Gene Expression Regulation Developmental Glucose Transporter Type 3 Humans Hydrogen Peroxide Hypoxia-Inducible Factor 1 alpha Subunit Imidazoles Membrane Proteins Oxygen Placentation Pregnancy Pregnancy Trimesters Primary Cell Culture Signal Transduction Transcription Genetic Vascular Endothelial Growth Factor A p38 Mitogen-Activated Protein Kinases
Robust cullin-RING ligase function is established by a multiplicity of poly-ubiquitylation pathways.
Spencer Hill, Kurt Reichermeier, Daniel C Scott, Lorena Samentar, Jasmin Coulombe-Huntington, Luisa Izzi, Xiaojing Tang, Rebeca Ibarra, Thierry Bertomeu, Annie Moradian, Michael J Sweredoski, Nora Caberoy, Brenda A Schulman, Frank Sicheri, Mike Tyers, Gary Kleiger
Jan 23, 2020
Biochemistry Cell Biology chemical biology cullin-RING ligase human protein degradation Ubiquitin ubiquitin ligases ubiquitin-conjugating enzyme Genome Human Humans Hypoxia-Inducible Factor 1 alpha Subunit Mass Spectrometry Polyubiquitin Signal Transduction Ubiquitin-Conjugating Enzymes Ubiquitin-Protein Ligases Ubiquitination
Pharmacological activation of a novel p53-dependent S-phase checkpoint involving CHK-1.
We have recently shown that induction of the p53 tumour suppressor protein by the small-molecule RITA (reactivation of p53 and induction of tumour cell apoptosis; 2,5-bis(5-hydroxymethyl-2-thienyl)furan) inhibits...
Apoptosis Ataxia Telangiectasia Breast Neoplasms Cell Cycle Proteins Cell Line Tumor Checkpoint Kinase 1 Colorectal Neoplasms DNA Damage DNA Replication Female Furans Gene Knockdown Techniques Histones Humans Hypoxia Hypoxia-Inducible Factor 1 alpha Subunit Osteosarcoma Phosphorylation Protein Kinase Inhibitors Protein Kinases Replication Origin S Phase Signal Transduction Transfection Tumor Suppressor Protein p53
Factor inhibiting HIF (FIH-1) promotes renal cancer cell survival by protecting cells from HIF-1α-mediated apoptosis.
MN Khan, T Bhattacharyya, P Andrikopoulos, MA Esteban, R Barod, T Connor, M Ashcroft, PH Maxwell, S Kiriakidis
Mar 21, 2019
BACKGROUND: Clear cell renal cell carcinoma (CCRCC) is the commonest form of kidney cancer. Up to 91% have biallelic inactivation of VHL, resulting in stabilisation of HIF-α subunits. Factor inhibiting HIF-1 is an enzyme that...
HIF1A and NFAT5 coordinate Na+-boosted antibacterial defense via enhanced autophagy and autolysosomal targeting
Patrick Neubert, Andrea Weichselbaum, Carmen Reitinger, Valentin Schatz, Agnes Schröder, John R Ferdinand, Michaela Simon, Anna-Lorena Bär, Christoph Brochhausen, Roman G Gerlach, Stefan Tomiuk, Karin Hammer, Stefan Wagner, Ger van Zandbergen, Katrina J Binger, Dominik N Müller, Kento Kitada, Menna R Clatworthy, Christian Kurts, Jens Titze, Zeinab Abdullah, Jonathan Jantsch
Apr 17, 2019
Autophagy cell-autonomous immunity macrophage salt sodium Animals Autophagosomes Autophagy Escherichia coli Homeodomain Proteins Hydrogen-Ion Concentration Hypoxia-Inducible Factor 1 alpha Subunit Inflammation Lysosomes MACROPHAGES Mannitol Mice Microscopy Electron Transmission Nitric Oxide Synthase Type II Oligonucleotide Array Sequence Analysis Osmotic Pressure Proto-Oncogene Proteins c-akt Reactive Oxygen Species sodium TOR Serine-Threonine Kinases Transcription Factors
Genetic approaches to understand cellular responses to oxygen availability.
Oxygen-sensing mechanisms have evolved to allow organisms to respond and adapt to oxygen availability. In metazoans, oxygen-sensing is predominantly mediated by the hypoxia inducible factors (HIFs). These transcription factors...
HIF isoforms in the skin differentially regulate systemic arterial pressure.
Andrew S Cowburn, Norihiko Takeda, Adam T Boutin, Jung-Whan Kim, Jane C Sterling, Manando Nakasaki, Mark Southwood, Ananda W Goldrath, Colin Jamora, Victor Nizet, Edwin R Chilvers, Randall S Johnson
Jun 19, 2018
Vascular flow through tissues is regulated via a number of homeostatic mechanisms. Localized control of tissue blood flow, or autoregulation, is a key factor in regulating tissue perfusion and oxygenation. We show here that the...
HIF-alpha Arginase vascular tone Adult Aged Animals Arginase Arterial Pressure Basic Helix-Loop-Helix Transcription Factors Blotting Western Female Humans Hypertension Hypoxia-Inducible Factor 1 alpha Subunit Immunohistochemistry Keratinocytes Male Mice Mice Inbred C57BL Mice Knockout Middle Aged Nitric Oxide Synthase Type II Nitric Oxide Synthase Type III Regional Blood Flow Reverse Transcriptase Polymerase Chain Reaction Skin Young Adult
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