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Results: 37
Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1.
Michael Prattes, Irina Grishkovskaya, Victor-Valentin Hodirnau, Christina Hetzmannseder, Gertrude Zisser, Carolin Sailer, Vasileios Kargas, Mathias Loibl, Magdalena Gerhalter, Lisa Kofler, Alan J Warren, Florian Stengel, David Haselbach, Helmut Bergler
Sep 23, 2022
The AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis that initiates cytoplasmic maturation of the large ribosomal subunit. Drg1 releases the shuttling maturation factor Rlp24 from pre-60S particles shortly after...
Terminating the replication helicase.
A feature of the cell cycle is that the events of one cycle must be reset before the next one begins. A study now shows that the replication machinery is removed from fully replicated DNA by a conserved ubiquitin- and CDC48...
MDA5 disease variant M854K prevents ATP-dependent structural discrimination of viral and cellular RNA.
Plasmids in the driving seat
Cell division checkpoint Indole Plasmid maintenance Regulatory RNA Signalling Adenosine Triphosphatases Bacterial Proteins Cation Transport Proteins Copper-Transporting ATPases DNA Replication Escherichia coli Escherichia coli Proteins Gene Expression Regulation Bacterial Indoles Plasmids Tryptophanase
Non-SMC condensin I complex proteins control chromosome segregation and survival of proliferating cells in the zebrafish neural retina.
Sabine Seipold, Florian C Priller, Paul Goldsmith, William A Harris, Herwig Baier, Salim Abdelilah-Seyfried
Jun 13, 2011
Quantitative Gait Analysis Using a Motorized Treadmill System Sensitively Detects Motor Abnormalities in Mice Expressing ATPase Defective Spastin.
A Conserved Mitochondrial ATP-binding Cassette Transporter Exports Glutathione Polysulfide for Cytosolic Metal Cofactor Assembly
ABC transporter Arabidopsis Glutathione Iron-Sulfur Protein Mitochondria Transportomics Yeast ATP-Binding Cassette Transporters Adenosine Triphosphatases Amino Acid Sequence Arabidopsis Arabidopsis Proteins Biological Transport Conserved Sequence Cytosol Glutathione Metals Mitochondrial Proteins Molecular Sequence Data Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sequence Homology Amino Acid Sulfides
Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis.
J He, HM Cooper, A Reyes, M Di Re, H Sembongi, TR Litwin, J Gao, KC Neuman, IM Fearnley, A Spinazzola, JE Walker, IJ Holt
Aug 11, 2017
ATPases Associated with Diverse Cellular Activities Adenosine Triphosphatases Cell Cycle Proteins Cell Line Tumor DNA Mitochondrial HEK293 Cells Humans Membrane Proteins Mitochondria Mitochondrial Proteins Nuclear Proteins Nucleoproteins Protein Biosynthesis RNA RNA Messenger RNA Mitochondrial Repressor Proteins Ribosomes
A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells.
BACKGROUND: Host factors required for retroviral infection are potential targets for the modulation of diseases caused by retroviruses. During the retroviral life cycle, numerous cellular factors interact with the virus and play...
The neurological and ophthalmological manifestations of SPG4-related hereditary spastic paraplegia.
Grant Guthrie, Gerald Pfeffer, Maura Bailie, Karen Bradshaw, Andrew C Browning, Rita Horvath, Patrick F Chinnery, Patrick Yu-Wai-Man
Mar 07, 2019
Turning the Mre11/Rad50 DNA repair complex on its head
The bacterial SbcC/SbcD DNA repair proteins were identified over a quarter of a century ago. Following the subsequent identification of the homologous Mre11/Rad50 complex in the eukaryotes and archaea, it has become clear that...
DNA Repair Mre11–Rad50 SMC Archaea Chromosomes Acid Anhydride Hydrolases Adenosine Triphosphatases Adenosine Triphosphate Archaeal Proteins Bacterial Proteins Cell Cycle DNA DNA Breaks Double-Stranded DNA Repair DNA-Binding Proteins Deoxyribonucleases Endodeoxyribonucleases Escherichia coli Proteins Exodeoxyribonucleases Exonucleases Humans Hydrolysis MRE11 Homologue Protein Mutation Protein Binding Protein Conformation Zinc
Structure of the hexameric HerA ATPase reveals a mechanism of translocation-coupled DNA-end processing in archaea.
Neil J Rzechorzek, John K Blackwood, Sian M Bray, Joseph D Maman, Luca Pellegrini, Nicholas P Robinson
Sep 30, 2014
Compounds activating VCP D1 ATPase enhance both autophagic and proteasomal neurotoxic protein clearance.
Lidia Wrobel, Sandra M Hill, Alvin Djajadikerta, Marian Fernandez-Estevez, Cansu Karabiyik, Avraham Ashkenazi, Victoria J Barratt, Eleanna Stamatakou, Anders Gunnarsson, Timothy Rasmusson, Eric W Miele, Nigel Beaton, Roland Bruderer, Yuehan Feng, Lukas Reiter, M Paola Castaldi, Rebecca Jarvis, Keith Tan, Roland W Bürli, David C Rubinsztein
Aug 16, 2022
Identification of rare sequence variation underlying heritable pulmonary arterial hypertension.
Stefan Gräf, Matthias Haimel, Marta Bleda, Charaka Hadinnapola, Laura Southgate, Wei Li, Joshua Hodgson, Bin Liu, Richard M Salmon, Mark Southwood, Rajiv D Machado, Jennifer M Martin, Carmen M Treacy, Katherine Yates, Louise C Daugherty, Olga Shamardina, Deborah Whitehorn, Simon Holden, Micheala Aldred, Harm J Bogaard, Colin Church, Gerry Coghlan, Robin Condliffe, Paul A Corris, Cesare Danesino, Mélanie Eyries, Henning Gall, Stefano Ghio, Hossein-Ardeschir Ghofrani, J Simon R Gibbs, Barbara Girerd, Arjan C Houweling, Luke Howard, Marc Humbert, David G Kiely, Gabor Kovacs, Robert V MacKenzie Ross, Shahin Moledina, David Montani, Michael Newnham, Andrea Olschewski, Horst Olschewski, Andrew J Peacock, Joanna Pepke-Zaba, Inga Prokopenko, Christopher J Rhodes, Laura Scelsi, Werner Seeger, Florent Soubrier, Dan F Stein, Jay Suntharalingam, Emilia M Swietlik, Mark R Toshner, David A van Heel, Anton Vonk Noordegraaf, Quinten Waisfisz, John Wharton, Stephen J Wort, Willem H Ouwehand, Nicole Soranzo, Allan Lawrie, Paul D Upton, Martin R Wilkins, Richard C Trembath, Nicholas W Morrell
May 21, 2018
Pulmonary arterial hypertension (PAH) is a rare disorder with a poor prognosis. Deleterious variation within components of the transforming growth factor-β pathway, particularly the bone morphogenetic protein type 2 receptor...
Adenosine Triphosphatases Adult Aquaporin 1 Base Sequence Bone Morphogenetic Protein Receptors Type II Case-Control Studies Familial Primary Pulmonary Hypertension Female Gene Expression Regulation Genetic Predisposition to Disease Growth Differentiation Factor 2 Growth Differentiation Factors HEK293 Cells Humans Male Membrane Transport Proteins Models Molecular Mutation Prognosis SOXF Transcription Factors Signal Transduction Transforming Growth Factor beta whole genome sequencing
The AAA+ protein ATAD3 has displacement loop binding properties and is involved in mitochondrial nucleoid organization.
J He, C-C Mao, A Reyes, H Sembongi, M Di Re, C Granycome, AB Clippingdale, IM Fearnley, M Harbour, AJ Robinson, S Reichelt, JN Spelbrink, JE Walker, IJ Holt
Aug 11, 2017
Adenosine Triphosphatases Adenosine Triphosphate Animals Binding Sites Binding Competitive Cell Line Tumor DNA Mitochondrial DNA Single-Stranded DNA Superhelical DNA-Binding Proteins Electrophoresis Gel Two-Dimensional Electrophoretic Mobility Shift Assay Humans Membrane Proteins Mitochondria Liver Mitochondrial Proteins Nucleic Acid Conformation Nucleoproteins Peptide Fragments Plasmids Protein Binding RNA Small Interfering Rats Submitochondrial Particles
P(I) Release Limits the Intrinsic and RNA-Stimulated ATPase Cycles of DEAD-Box Protein 5 (Dbp5).
Emily V Wong, Wenxiang Cao, Judit Vörös, Monique Merchant, Yorgo Modis, David D Hackney, Ben Montpetit, Enrique M De La Cruz
Aug 09, 2017
Tuning the drug efflux activity of an ABC transporter in vivo by in vitro selected DARPin binders.
Markus A Seeger, Anshumali Mittal, Saroj Velamakanni, Michael Hohl, Stefan Schauer, Ihsene Salaa, Markus G Grütter, Hendrik W van Veen
Oct 05, 2020
ABC transporters use the energy from binding and hydrolysis of ATP to import or extrude substrates across the membrane. Using ribosome display, we raised designed ankyrin repeat proteins (DARPins) against detergent solubilized...
ATP-Binding Cassette Transporters Adenosine Triphosphatases Ankyrin Repeat Biological Transport Biophysical Phenomena Cell Membrane Daunorubicin detergents Enzyme-Linked Immunosorbent Assay Epitope Mapping Epitopes Lactococcus lactis Pharmaceutical Preparations Protein Binding Proteolipids Recombinant Proteins Ribosomes Solubility Surface Plasmon Resonance
Calcium depletion challenges endoplasmic reticulum proteostasis by destabilising BiP-substrate complexes.
BiP Grp78 Biochemistry Calcium Cell Biology chemical biology Endoplasmic Reticulum None oligomerisation Adenosine Diphosphate Adenosine Triphosphatases Animals CHO Cells Calcium Cricetulus Crystallography X-Ray Drosophila Endoplasmic Reticulum Endoplasmic Reticulum Chaperone BiP Escherichia coli Flow Cytometry HSP70 Heat-Shock Proteins HEAT-SHOCK PROTEINS Immunoprecipitation Proteostasis Unfolded Protein Response
Arabidopsis MORC proteins function in the efficient establishment of RNA directed DNA methylation.
Yan Xue, Zhenhui Zhong, C Jake Harris, Javier Gallego-Bartolomé, Ming Wang, Colette Picard, Xueshi Cao, Shan Hua, Ivy Kwok, Suhua Feng, Yasaman Jami-Alahmadi, Jihui Sha, Jason Gardiner, James Wohlschlegel, Steven E Jacobsen
Sep 13, 2021
The Microrchidia (MORC) family of ATPases are required for transposable element (TE) silencing and heterochromatin condensation in plants and animals, and C. elegans MORC-1 has been shown to topologically entrap and condense...
Clamping of DNA shuts the condensin neck gate.
SignificanceDNA needs to be compacted to fit into nuclei and during cell division, when dense chromatids are formed for their mechanical segregation, a process that depends on the protein complex condensin. It forms and enlarges...
Mi-2/NuRD complex protects stem cell progeny from mitogenic Notch signaling.
To progress towards differentiation, progeny of stem cells need to extinguish expression of stem-cell maintenance genes. Failures in such mechanisms can drive tumorigenesis. In Drosophila neural stem cell (NSC) lineages...
D. melanogaster Notch NuRD Complex Chromosomes developmental biology Gene Expression Stem Cells Adenosine Triphosphatases Animals Autoantigens Basic Helix-Loop-Helix Transcription Factors Carcinogenesis Cell Differentiation Cell Lineage Cell Proliferation Drosophila Proteins Drosophila melanogaster Gene Expression Regulation Developmental Humans Hyperplasia Models Biological Neural Stem Cells Neurons Protein Binding Receptors Notch Repressor Proteins Signal Transduction
Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2
IA Tchasovnikarova, RT Timms, CH Douse, RC Roberts, G Dougan, RE Kingston, Y Modis, PJ Lehner
Jul 13, 2017
Adenosine Triphosphatases CRISPR-Cas Systems Charcot-Marie-Tooth disease Chromatin Assembly and Disassembly Epigenetic Repression Gene Silencing HeLa Cells Heterochromatin Histone Code Histone-Lysine N-Methyltransferase Histones Humans Lysine Methylation Multiprotein Complexes Mutation Missense Neurons Protein Domains Protein Interaction Mapping Protein Methyltransferases Protein Processing Post-Translational Transcription Factors Transgenes
Spastin couples microtubule severing to membrane traffic in completion of cytokinesis and secretion.
Mutations in the gene encoding the microtubule (MT)-severing protein spastin are the most common cause of hereditary spastic paraplegia, a genetic condition in which axons of the corticospinal tracts degenerate. We show that not...
Folding of cohesin's coiled coil is important for Scc2/4-induced association with chromosomes.
Naomi J Petela, Andres Gonzalez Llamazares, Sarah Dixon, Bin Hu, Byung-Gil Lee, Jean Metson, Heekyo Seo, Antonio Ferrer-Harding, Menelaos Voulgaris, Thomas Gligoris, James Collier, Byung-Ha Oh, Jan Löwe, Kim A Nasmyth
Jul 28, 2021
Cohesin's association with and translocation along chromosomal DNAs depend on an ATP hydrolysis cycle driving the association and subsequent release of DNA. This involves DNA being 'clamped' by Scc2 and ATP-dependent engagement...
S. cerevisiae Chromosomes cryo-EM Folding Gene Expression molecular biophysics sister chromatid cohesion structural biology Adenosine Triphosphatases Cell Cycle Proteins Chromatids Chromosomal Proteins Non-Histone Chromosomes Cryoelectron Microscopy DNA Dimerization Gene Expression Regulation Fungal Hydrolysis Protein Domains Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins
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