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Self-organization of swimmers drives long-range fluid transport in bacterial colonies.
Motile subpopulations in microbial communities are believed to be important for dispersal, quest for food, and material transport. Here, we show that motile cells in sessile colonies of peritrichously flagellated bacteria can...
Donor-strand exchange drives assembly of the TasA scaffold in Bacillus subtilis biofilms.
Jan Böhning, Mnar Ghrayeb, Conrado Pedebos, Daniel K Abbas, Syma Khalid, Liraz Chai, Tanmay AM Bharat
Dec 19, 2022
Many bacteria in nature exist in multicellular communities termed biofilms, where cells are embedded in an extracellular matrix that provides rigidity to the biofilm and protects cells from chemical and mechanical stresses. In...
Diffusion-Limited Growth of Microbial Colonies.
Hayden Tronnolone, Alexander Tam, Zoltán Szenczi, JEF Green, Sanjeeva Balasuriya, Ee Lin Tek, Jennifer M Gardner, Joanna F Sundstrom, Vladimir Jiranek, Stephen G Oliver, Benjamin J Binder
Sep 20, 2018
The emergence of diffusion-limited growth (DLG) within a microbial colony on a solid substrate is studied using a combination of mathematical modelling and experiments. Using an agent-based model of the interaction between...
Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus subtilis Cells upon Prophage Induction During Spore Germination, Outgrowth, and Vegetative Growth Using Raman Tweezers and Live-Cell Phase-Contrast Microscopy.
High molecular weight DNA assembly in vivo for synthetic biology applications.
Paramagnetism in Bacillus spores
Ke Xu Zhou, Adrian Ionescu, Eamon Wan, Yeuk N Ho, Crispin HW Barnes, Graham Christie, D Ian Wilson
Feb 08, 2018
Spores of Bacillus megaterium, Bacillus cereus, and Bacillus subtilis were found to exhibit intrinsic paramagnetic properties as a result of the accumulation of manganese ions. All three Bacillus species displayed strong yet...
A Comparative Analysis of the Core Proteomes within and among the Bacillus subtilis and Bacillus cereus Evolutionary Groups Reveals the Patterns of Lineage- and Species-Specific Adaptations.
Marios Nikolaidis, Andrew Hesketh, Dimitris Mossialos, Ioannis Iliopoulos, Stephen G Oliver, Grigorios D Amoutzias
Sep 02, 2022
By integrating phylogenomic and comparative analyses of 1104 high-quality genome sequences, we identify the core proteins and the lineage-specific fingerprint proteins of the various evolutionary clusters (clades/groups/species)...
A Comparative Analysis of the Core Proteomes within and among the Bacillus subtilis and Bacillus cereus Evolutionary Groups Reveals the Patterns of Lineage- and Species-Specific Adaptations.
Marios Nikolaidis, Andrew Hesketh, Dimitris Mossialos, Ioannis Iliopoulos, Stephen G Oliver, Grigorios D Amoutzias
Oct 24, 2022
By integrating phylogenomic and comparative analyses of 1104 high-quality genome sequences, we identify the core proteins and the lineage-specific fingerprint proteins of the various evolutionary clusters (clades/groups/species)...
DNA Nanostructures for Targeted Antimicrobial Delivery.
Ioanna Mela, Pedro P Vallejo-Ramirez, Stanislaw Makarchuk, Graham Christie, David Bailey, Robert M Henderson, Hiroshi Sugiyama, Masayuki Endo, Clemens F Kaminski
Apr 17, 2020
We report the use of DNA origami nanostructures, functionalized with aptamers, as a vehicle for delivering the antibacterial enzyme lysozyme in a specific and efficient manner. We test the system against Gram-positive (Bacillus...
DNA nanostructures antimicrobial Atomic force microscopy bionanotechnology dSTORM Animals Anti-Bacterial Agents Aptamers Nucleotide Bacillus subtilis COS Cells Chlorocebus aethiops DNA Drug Carriers Enzymes Immobilized Escherichia coli Microbial Sensitivity Tests Muramidase NANOSTRUCTURES Nucleic Acid Conformation
Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR.
Gottfried J Palm, Bui Khanh Chi, Paul Waack, Katrin Gronau, Dörte Becher, Dirk Albrecht, Winfried Hinrichs, Randy J Read, Haike Antelmann
May 11, 2020
Bacillus subtilis encodes redox-sensing MarR-type regulators of the OhrR and DUF24-families that sense organic hydroperoxides, diamide, quinones or aldehydes via thiol-based redox-switches. In this article, we characterize the...
Bacillus subtilis Bacterial Proteins Base Sequence Cysteine DNA-Binding Proteins Diamide Gene Expression Regulation Bacterial Models Molecular Molecular Sequence Data Nitroreductases Operator Regions Genetic Oxidation-Reduction Oxidoreductases Sodium Hypochlorite Stress Physiological Trans-Activators Transcriptional Activation
A system for the expression and release of heterologous proteins from the core of Bacillus subtilis spores.
Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs.
Stephanie Oerum, Tom Dendooven, Marjorie Catala, Laetitia Gilet, Clément Dégut, Aude Trinquier, Maxime Bourguet, Pierre Barraud, Sarah Cianferani, Ben F Luisi, Ciarán Condon, Carine Tisné
Jan 06, 2021
The pathways for ribosomal RNA (rRNA) maturation diverge greatly among the domains of life. In the Gram-positive model bacterium, Bacillus subtilis, the final maturation steps of the two large ribosomal subunit (50S) rRNAs, 23S...
Lambda Red recombinase-mediated integration of the high molecular weight DNA into the Escherichia coli chromosome.
ATP Analogues for Structural Investigations
Denis Lacabanne, Thomas Wiegand, Nino Wili, Maria I Kozlova, Riccardo Cadalbert, Daniel Klose, Armen Y Mulkidjanian, Beat H Meier, Anja Böckmann
Nov 13, 2020
Nucleoside triphosphates (NTPs) are used as chemical energy source in a variety of cell systems. Structural snapshots along the NTP hydrolysis reaction coordinate are typically obtained by adding stable, nonhydrolyzable...
ABC transporter ATP analogues ATP hydrolysis DnaB helicase ELDOR-detected NMR Solid-state NMR ATP-Binding Cassette Transporters Adenosine Triphosphatases Adenosine Triphosphate Adenylyl Imidodiphosphate Aluminum Compounds Bacillus subtilis Bacterial Proteins DnaB Helicases Electron Spin Resonance Spectroscopy ELECTRONS Fluorides Helicobacter pylori Hydrolysis Magnetic Resonance Spectroscopy Protein Conformation
Stochastic pulsing of gene expression enables the generation of spatial patterns in Bacillus subtilis biofilms.
Stochastic pulsing of gene expression can generate phenotypic diversity in a genetically identical population of cells, but it is unclear whether it has a role in the development of multicellular systems. Here, we show how...
Tunable phenotypic variability through an autoregulatory alternative sigma factor circuit
Christian P Schwall, Torkel E Loman, Bruno M C Martins, Sandra Cortijo, Casandra Villava, Vassili Kusmartsev, Toby Livesey, Teresa Saez, James C W Locke
Jul 28, 2021
EMBO23 article ARTICLES Bacillus subtilis microbial systems biology single‐cell time‐lapse microscopy stochastic gene expression stress priming
Published by: Molecular Systems Biology
Integrative bacterial artificial chromosomes for DNA integration into the Bacillus subtilis chromosome.
Bacterial artificial chromosome Chromosomal integration RecA homologous recombination mVenus λ red recombineering Bacillus subtilis Chromosomes Artificial Bacterial Chromosomes Bacterial Escherichia coli Genetic Engineering Plasmids Rec A Recombinases Recombination Genetic synthetic biology Transformation Bacterial
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