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Results: 22
Outer membrane β-barrel protein folding is physically controlled by periplasmic lipid head groups and BamA.
Dennis Gessmann, Yong Hee Chung, Emily J Danoff, Ashlee M Plummer, Clifford W Sandlin, Nathan R Zaccai, Karen G Fleming
Jul 04, 2016
beta-barrel transmembrane protein membrane protein folding Bacterial Outer Membrane Proteins Biocatalysis Cell Membrane Escherichia coli Escherichia coli Proteins Kinetics Lipids Models Biological Periplasm Phenylalanine Phosphatidylethanolamines Phosphatidylglycerols Protein Folding Protein Structure Secondary
Structure of an atypical periplasmic adaptor from a multidrug efflux pump of the spirochete Borrelia burgdorferi.
Nicholas P Greene, Philip Hinchliffe, Allister Crow, Abdessamad Ababou, Colin Hughes, Vassilis Koronakis
Sep 19, 2014
Adaptor protein antibiotic resistance Crystal structure HME IM MD MDR MP Multidrug efflux OM RMSDs heavy metal efflux inner membrane membrane proximal Molecular dynamics multidrug resistance outer membrane root mean square deviations Bacterial Outer Membrane Proteins Borrelia burgdorferi Crystallography X-Ray Escherichia coli Gene Expression Molecular Dynamics Simulation Multidrug Resistance-Associated Proteins Periplasm Protein Structure Secondary Protein Structure Tertiary Recombinant Proteins Selenomethionine
Structure of the MacAB-TolC ABC-type tripartite multidrug efflux pump
AWP Fitzpatrick, S Llabrés, A Neuberger, JN Blaza, X-C Bai, U Okada, S Murakami, HW van Veen, U Zachariae, SHW Scheres, BF Luisi, D Du
Aug 11, 2017
In situ structure and assembly of the multidrug efflux pump AcrAB-TolC.
Xiaodong Shi, Muyuan Chen, Zhili Yu, James M Bell, Hans Wang, Isaac Forrester, Heather Villarreal, Joanita Jakana, Dijun Du, Ben F Luisi, Steven J Ludtke, Zhao Wang
Jun 13, 2019
Anti-Bacterial Agents Bacterial Outer Membrane Proteins Carrier Proteins Cryoelectron Microscopy Drug Resistance Multiple Bacterial Electron Microscope Tomography Escherichia coli Escherichia coli Proteins Intravital Microscopy Lipoproteins Membrane Transport Proteins Multidrug Resistance-Associated Proteins Peptidoglycan Periplasm Protein Binding Protein Structure Quaternary
Whole-genome analysis of diverse Chlamydia trachomatis strains identifies phylogenetic relationships masked by current clinical typing.
Simon R Harris, Ian N Clarke, Helena MB Seth-Smith, Anthony W Solomon, Lesley T Cutcliffe, Peter Marsh, Rachel J Skilton, Martin J Holland, David Mabey, Rosanna W Peeling, David A Lewis, Brian G Spratt, Magnus Unemo, Kenneth Persson, Carina Bjartling, Robert Brunham, Henry JC de Vries, Servaas A Morré, Arjen Speksnijder, Cécile M Bébéar, Maïté Clerc, Bertille de Barbeyrac, Julian Parkhill, Nicholas R Thomson
Sep 06, 2019
Chlamydia trachomatis is responsible for both trachoma and sexually transmitted infections, causing substantial morbidity and economic cost globally. Despite this, our knowledge of its population and evolutionary genetics is...
Bacterial Outer Membrane Proteins Base Sequence Chlamydia Infections Chlamydia trachomatis DNA Bacterial Gene Transfer Horizontal Genome Bacterial Humans Lymphogranuloma Venereum Molecular Sequence Data Phylogeny Plasmids Polymorphism Single Nucleotide RNA Ribosomal 16S Recombination Genetic Sequence Analysis DNA Trachoma
Reconstitution of surface lipoprotein translocation through the Slam translocon.
Minh Sang Huynh, Yogesh Hooda, Yuzi Raina Li, Maciej Jagielnicki, Christine Chieh-Lin Lai, Trevor F Moraes
May 30, 2022
Surface lipoproteins (SLPs) are peripherally attached to the outer leaflet of the outer membrane in many Gram-negative bacteria, playing significant roles in nutrient acquisition and immune evasion in the host. While the factors...
Chaperones Skp and SurA dynamically expand unfolded OmpX and synergistically disassemble oligomeric aggregates.
Neharika Chamachi, Andreas Hartmann, Mai Quynh Ma, Anna Svirina, Georg Krainer, Michael Schlierf
Mar 29, 2022
Periplasmic chaperones 17-kilodalton protein (Skp) and survival factor A (SurA) are essential players in outer membrane protein (OMP) biogenesis. They prevent unfolded OMPs from misfolding during their passage through the...
In situ structure of the AcrAB-TolC efflux pump at subnanometer resolution.
Muyuan Chen, Xiaodong Shi, Zhili Yu, Guizhen Fan, Irina I Serysheva, Matthew L Baker, Ben F Luisi, Steven J Ludtke, Zhao Wang
Sep 16, 2021
The tripartite AcrAB-TolC assembly, which spans both the inner and outer membranes in Gram-negative bacteria, is an efflux pump that contributes to multidrug resistance. Here, we present the in situ structure of full-length...
AcrAB-TolC cryo-ET in situ multidrug efflux pump subnanometer resolution Allosteric Regulation Bacterial Outer Membrane Proteins Carrier Proteins Cryoelectron Microscopy Electron Microscope Tomography Escherichia coli Escherichia coli Proteins Ligands Lipoproteins Membrane Transport Proteins Models Molecular Multidrug Resistance-Associated Proteins Protein Conformation
Breaching the Barrier
The double-membrane cell envelope of Gram-negative bacteria is a sophisticated barrier that facilitates the uptake of nutrients and protects the organism from toxic compounds. An antibiotic molecule must find its way through the...
antibiotic resistance efflux envelope permeability OUTER-MEMBRANE PROTEINS Porins Anti-Bacterial Agents Bacterial Outer Membrane Bacterial Outer Membrane Proteins Biological Transport Cell Membrane Permeability Electrophysiology Gram-Negative Bacteria Hydrophobic and Hydrophilic Interactions Lipopolysaccharides Liposomes Microscopy Models Molecular Permeability Porins
Photoreduction of Shewanella oneidensis Extracellular Cytochromes by Organic Chromophores and Dye-Sensitized TiO2.
Emma V Ainsworth, Colin WJ Lockwood, Gaye F White, Ee Taek Hwang, Tsubasa Sakai, Manuela A Gross, David J Richardson, Thomas A Clarke, Lars JC Jeuken, Erwin Reisner, Julea N Butt
Nov 30, 2016
cytochromes electron transfer heme proteins photocatalysis photoreduction Bacterial Outer Membrane Proteins Catalysis Coloring Agents Cytochrome c Group Electron Transport Eosine Yellowish-(YS) Ferric Compounds Flavin Mononucleotide LIGHT Metal Nanoparticles Oxidation-Reduction Photosensitizing Agents Shewanella titanium
Structure and mechanotransmission mechanism of the MacB ABC transporter superfamily.
MacB is an ABC transporter that collaborates with the MacA adaptor protein and TolC exit duct to drive efflux of antibiotics and enterotoxin STII out of the bacterial cell. Here we present the structure of ATP-bound MacB and...
ABC transporter X-ray crystallography tripartite efflux pump ATP-Binding Cassette Transporters Adenosine Triphosphate Aggregatibacter actinomycetemcomitans Bacterial Outer Membrane Proteins Binding Sites Biological Transport Cell Cycle Proteins Crystallography X-Ray Cystic Fibrosis Transmembrane Conductance Regulator Escherichia coli Escherichia coli Proteins Gene Expression Models Molecular Protein Binding Protein Conformation alpha-Helical Protein Conformation beta-Strand Protein Interaction Domains and Motifs Protein Multimerization Recombinant Proteins Substrate Specificity
Pharyngeal carriage of Neisseria species in the African meningitis belt.
Kanny Diallo, Caroline Trotter, Youssouf Timbine, Boubou Tamboura, Samba O Sow, Bassira Issaka, Ibrahim D Dano, Jean-Marc Collard, Marietou Dieng, Aldiouma Diallo, Adane Mihret, Oumer A Ali, Abraham Aseffa, Stephen L Quaye, Akalifa Bugri, Isaac Osei, Kadidja Gamougam, Lodoum Mbainadji, Doumagoum M Daugla, Galadima Gadzama, Zailani B Sambo, Babatunji A Omotara, Julia S Bennett, Lisa S Rebbetts, Eleanor R Watkins, Maria Nascimento, Arouna Woukeu, Olivier Manigart, Ray Borrow, James M Stuart, Brian M Greenwood, Martin CJ Maiden
Apr 08, 2016
African meningitis belt Non-meningococcal Neisseria Pharyngeal carriage Adolescent Adult Africa Bacterial Outer Membrane Proteins Carrier State Child Child Preschool Female genetic variation Humans Infant Male Meningitis Meningococcal Meningococcal Infections Neisseria Neisseria meningitidis Pharynx prevalence Risk Factors Young Adult
MacB ABC transporter is a dimer whose ATPase activity and macrolide-binding capacity are regulated by the membrane fusion protein MacA.
Hong Ting Lin, Vassiliy N Bavro, Nelson P Barrera, Helen M Frankish, Saroj Velamakanni, Hendrik W van Veen, Carol V Robinson, M Inês Borges-Walmsley, Adrian R Walmsley
Oct 06, 2020
Gram-negative bacteria utilize specialized machinery to translocate drugs and protein toxins across the inner and outer membranes, consisting of a tripartite complex composed of an inner membrane secondary or primary active...
Structural basis of lipoprotein recognition by the bacterial Lol trafficking chaperone LolA.
In gram-negative bacteria, lipoproteins are vital structural components of the outer membrane (OM) and crucial elements of machineries central to the physiology of the cell envelope. A dedicated apparatus, the Lol system, is...
ABC transporter Lol lipoprotein trafficking X-ray crystallography antibacterial target protein–lipid interaction Bacterial Outer Membrane Proteins Carrier Proteins Escherichia coli Escherichia coli Proteins Ligands Lipoproteins Models Molecular Molecular Chaperones Periplasm Periplasmic Binding Proteins Protein Structure Tertiary Protein Transport
Dynamics and impact of homologous recombination on the evolution of Legionella pneumophila.
Sophia David, Leonor Sánchez-Busó, Simon R Harris, Pekka Marttinen, Christophe Rusniok, Carmen Buchrieser, Timothy G Harrison, Julian Parkhill
Oct 23, 2019
Legionella pneumophila is an environmental bacterium and the causative agent of Legionnaires' disease. Previous genomic studies have shown that recombination accounts for a high proportion (>96%) of diversity within several...
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants.
Rhys Grinter, Iain D Hay, Jiangning Song, Jiawei Wang, Don Teng, Vijay Dhanesakaran, Jonathan J Wilksch, Mark R Davies, Dene Littler, Simone A Beckham, Ian R Henderson, Richard A Strugnell, Gordon Dougan, Trevor Lithgow
Nov 21, 2018
Iron is essential for life. Accessing iron from the environment can be a limiting factor that determines success in a given environmental niche. For bacteria, access of chelated iron from the environment is often mediated by...
MexAB-OprM Efflux Pump Interaction with the Peptidoglycan of Escherichia coli and Pseudomonas aeruginosa.
Miao Ma, Margaux Lustig, Michèle Salem, Dominique Mengin-Lecreulx, Gilles Phan, Isabelle Broutin
Jul 07, 2021
One of the major families of membrane proteins found in prokaryote genome corresponds to the transporters. Among them, the resistance-nodulation-cell division (RND) transporters are highly studied, as being responsible for one...
Pseudomonas efflux pump assembly membrane Peptidoglycan resistance Bacterial Outer Membrane Proteins Cell Wall Drug Resistance Bacterial Escherichia coli Membrane Transport Proteins Models Molecular Peptidoglycan Protein Interaction Domains and Motifs Protein Stability Protein Structure Quaternary Pseudomonas aeruginosa
Genomic Profiling Reveals Distinct Routes To Complement Resistance in Klebsiella pneumoniae.
Francesca L Short, Gianna Di Sario, Nathalie T Reichmann, Colin Kleanthous, Julian Parkhill, Peter W Taylor
Jun 22, 2020
The serum complement system is a first line of defense against bacterial invaders. Resistance to killing by serum enhances the capacity of Klebsiella pneumoniae to cause infection, but it is an incompletely understood virulence...
Klebsiella Tn-Seq TraDIS capsule complement resistance Functional genomics serum resistance transposon insertion sequencing Bacterial Outer Membrane Proteins Blood Bactericidal Activity Carboxy-Lyases Complement C3b Complement Membrane Attack Complex DNA Transposable Elements Gene Expression Profiling Gene Expression Regulation Bacterial Gene Library Genes Bacterial Humans Immune Evasion Klebsiella Infections Klebsiella pneumoniae Mutation Peptide Elongation Factors Sequence Analysis DNA
Evaluation of Reactivity of Monoclonal Antibodies Against Omp25 of Brucella spp.
Xin Yang, Zuoping He, Guoxia Zhang, Jinhui Lu, Hui Zhang, Hui Ren, Yanjun Tian, Heng Yang, Chuangfu Chen, Linhai Li, Yongshui Fu, Jean-Pierre Allain, Chengyao Li, Wenjing Wang
May 05, 2020
Brucellosis is a serious zoonosis occurring mainly in developing countries, and its diagnosis is largely dependent on serologic detection and bacterial culture. In this study, we developed the murine monoclonal antibodies (mAbs)...
Emergence of dominant toxigenic M1T1 Streptococcus pyogenes clone during increased scarlet fever activity in England
Nicola N Lynskey, Elita Jauneikaite, Ho Kwong Li, Xiangyun Zhi, Claire E Turner, Mia Mosavie, Max Pearson, Masanori Asai, Ludmila Lobkowicz, J Yimmy Chow, Julian Parkhill, Theresa Lamagni, Victoria J Chalker, Shiranee Sriskandan
Oct 01, 2019
BACKGROUND: Since 2014, England has seen increased scarlet fever activity unprecedented in modern times. In 2016, England's scarlet fever seasonal rise coincided with an unexpected elevation in invasive Streptococcus pyogenes...
Adolescent Adult Aged Aged 80 and over Antigens Bacterial Bacteremia Bacterial Outer Membrane Proteins Bacterial Proteins Carrier Proteins Child Child Preschool England Exotoxins Female Gene Expression Profiling Genotype Humans Incidence Infant Infant Newborn Male Membrane Proteins Middle Aged molecular epidemiology scarlet fever Streptococcus pyogenes Young Adult
The Emergence of Successful Streptococcus pyogenes Lineages through Convergent Pathways of Capsule Loss and Recombination Directing High Toxin Expression.
Claire E Turner, Matthew TG Holden, Beth Blane, Carolyne Horner, Sharon J Peacock, Shiranee Sriskandan
Nov 14, 2019
Gene transfer and homologous recombination in Streptococcus pyogenes has the potential to trigger the emergence of pandemic lineages, as exemplified by lineages of emm1 and emm89 that emerged in the 1980s and 2000s...
NADase Streptococcus pyogenes convergent evolution group A Streptococcus homologous recombination hyaluronic acid capsule streptolysin O whole-genome sequencing Antigens Bacterial Bacterial Capsules Bacterial Outer Membrane Proteins Bacterial Proteins Base Sequence England Genotype Humans NAD+ Nucleosidase Streptococcal Infections Streptococcus pyogenes Streptolysins Toxins Biological Virulence Factors
Bacteriophage host range evolution through engineered enrichment bias, exploiting heterologous surface receptor expression.
Research on the initial phage-host interaction has been conducted on a limited repertoire of phages and their cognate receptors, such as phage λ and the Escherichia coli LamB (EcLamB) protein. Apart from phage λ, little is known...