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Human Stem Cell-Derived Endothelial-Hepatic Platform for Efficacy Testing of Vascular-Protective Metabolites from Nutraceuticals.
Balakrishnan Chakrapani Narmada, Yeek Teck Goh, Huan Li, Sanjay Sinha, Hanry Yu, Christine Cheung
Feb 07, 2019
Atherosclerosis underlies many cardiovascular and cerebrovascular diseases. Nutraceuticals are emerging as a therapeutic moiety for restoring vascular health. Unlike small-molecule drugs, the complexity of ingredients in...
Coculture Endothelial Cells Hepatocytes Human pluripotent stem cells metabolism Nutraceutical testing Culture Media Conditioned Dietary Supplements endothelial progenitor cells Genistein Hepatocytes Human Embryonic Stem Cells Humans Inflammation Interleukin-1beta Liver Metabolome Pluripotent Stem Cells Protective Agents Quercetin
DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids.
Sebastian Canovas, Elena Ivanova, Raquel Romar, Soledad García-Martínez, Cristina Soriano-Úbeda, Francisco A García-Vázquez, Heba Saadeh, Simon Andrews, Gavin Kelsey, Pilar Coy
Jun 24, 2019
The number of children born since the origin of Assisted Reproductive Technologies (ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been reported among ART-conceived infants, suggesting...
SDF-1 chemokine signalling modulates the apoptotic responses to iron deprivation of clathrin-depleted DT40 cells.
Alena Pance, Frank R Morrissey-Wettey, Helen Craig, Alison Downing, Richard Talbot, Antony P Jackson
Jan 15, 2016
Animals Apoptosis Cell Line Cell Survival Chemokine CXCL12 Chickens Clathrin Heavy Chains Culture Media Gene Expression Profiling Gene Expression Regulation Iron Deficiencies Oligonucleotide Array Sequence Analysis Precursor Cells B-Lymphoid Promoter Regions Genetic Receptors CXCR4 Signal Transduction Tetracycline Transferrin
Toxicity and biofilm-based selection for methyl tert-butyl ether bioremediation technology.
Extractive membrane biofilm reactor (EMBFR) technology offers productive solutions for volatile and semi-volatile compound removal from water bodies. In this study, the bacterial strains Paenibacillus etheri SH7T (CECT 8558)...
Computer-Aided Strategies for Determining the Amino Acid Composition of Medium for Chinese Hamster Ovary Cell-Based Biomanufacturing Platforms.
Chinese hamster ovary (CHO) cells are used for the production of the majority of biopharmaceutical drugs, and thus have remained the standard industry host for the past three decades. The amino acid composition of the medium...
The effect of 2 mMol glutamine supplementation on HSP70 and TNF-α release by LPS stimulated blood from healthy children.
Comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents.
Theodore Gouliouris, Beth Blane, Hayley J Brodrick, Kathy E Raven, Kirsty E Ambridge, Angela D Kidney, Nazreen F Hadjirin, M Estée Török, Direk Limmathurotsakul, Sharon J Peacock
May 13, 2016
detection Enterococcus faecium Selectivity sensitivity VRE Vancomycin-Resistant Enterococci Bacteriological Techniques Carrier State Chromogenic Compounds Culture Media Enterococcus faecalis Enterococcus faecium Feces Gram-Positive Bacterial Infections Humans Nursing Homes Prospective Studies Sensitivity and Specificity Vancomycin-Resistant Enterococci
Expanded potential stem cell media as a tool to study human developmental hematopoiesis in vitro.
Adam C Wilkinson, David J Ryan, Iwo Kucinski, Wei Wang, Jian Yang, Sonia Nestorowa, Evangelia Diamanti, Jason Cheuk-Ho Tsang, Juexuan Wang, Lia S Campos, Fengtang Yang, Beiyuan Fu, Nicola Wilson, Pentao Liu, Berthold Gottgens
Jul 16, 2019
Animals Cell Culture Techniques Cell Cycle Cell Lineage Cells Cultured Cellular Reprogramming Techniques Culture Media Embryoid Bodies FIBROBLASTS Genes Reporter Hematopoiesis Human Embryonic Stem Cells Humans Mice Neural Stem Cells Pluripotent Stem Cells Sequence Analysis RNA Species Specificity Stem Cell Transplantation Teratoma
Indole Pulse Signalling Regulates the Cytoplasmic pH of E. coli in a Memory-Like Manner.
Ashraf Zarkan, Santiago Caño-Muñiz, Jinbo Zhu, Kareem Al Nahas, Jehangir Cama, Ulrich F Keyser, David K Summers
Feb 19, 2019
Secreted factors from olfactory mucosa cells expanded as free-floating spheres increase neurogenesis in olfactory bulb neurosphere cultures.
Animals Animals Newborn Calbindin 2 Cell Proliferation Cells Cultured Culture Media Conditioned Glial Fibrillary Acidic Protein Immunohistochemistry Mice Nerve Growth Factors Nerve Tissue Proteins Neuregulin-1 Neuroglia Neurons Olfactory Bulb Olfactory Mucosa Phenotype S100 Calcium Binding Protein G Spheroids Cellular Stem Cells Tubulin gamma-Aminobutyric Acid
Alternative Molecular Methods for Improved Detection of Meningococcal Carriage and Measurement of Bacterial Density.
Olivier Manigart, Jacinta Okeakpu, Aderonke Odutola, Sheikh Jarju, Ebenezer Foster-Nyarko, Kanny Diallo, Anna Roca, Beate Kampmann, Umberto D'Alessandro, Samba Sow, Martin Antonio, Martin J Maiden, Ray Borrow, James M Stuart, Caroline L Trotter, Brian M Greenwood
Oct 18, 2016
Free-energy-based framework for early forecasting of stem cell differentiation.
Commitment of stem cells to different lineages is inherently stochastic but regulated by a range of environmental bio/chemo/mechanical cues. Here, we develop an integrated stochastic modelling framework for predicting the...
A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions.
Itsunari Minami, Kohei Yamada, Tomomi G Otsuji, Takuya Yamamoto, Yan Shen, Shinya Otsuka, Shin Kadota, Nobuhiro Morone, Maneesha Barve, Yasuyuki Asai, Tatyana Tenkova-Heuser, John E Heuser, Motonari Uesugi, Kazuhiro Aiba, Norio Nakatsuji
May 28, 2019
Human pluripotent stem cells (hPSCs), including embryonic stem cells and induced pluripotent stem cells, are potentially useful in regenerative therapies for heart disease. For medical applications, clinical-grade cardiac cells...
Low rates of mutation in clinical grade human pluripotent stem cells under different culture conditions.
Oliver Thompson, Ferdinand von Meyenn, Zoe Hewitt, John Alexander, Andrew Wood, Richard Weightman, Sian Gregory, Felix Krueger, Simon Andrews, Ivana Barbaric, Paul J Gokhale, Harry D Moore, Wolf Reik, Marta Milo, Serena Nik-Zainal, Kosuke Yusa, Peter W Andrews
Feb 27, 2020
The occurrence of repetitive genomic changes that provide a selective growth advantage in pluripotent stem cells is of concern for their clinical application. However, the effect of different culture conditions on the underlying...
Organoid culture media formulated with growth factors of defined cellular activity.
Manuela Urbischek, Helena Rannikmae, Thomas Foets, Katharina Ravn, Marko Hyvönen, Marc de la Roche
Apr 15, 2019
The media formulations necessary for deriving and sustaining organoids from epithelial tissues such as prostate, colon, gastric, liver, pancreas, and others have been established. Critical components of organoid media are a set...
LPS ligand and culture additives improve production of monomeric MD-1 and 2 in Pichia pastoris by decreasing aggregation and intermolecular disulfide bonding.
In situ magnetic separation of antibody fragments from Escherichia coli in complex media.
Martin Cerff, Alexander Scholz, Matthias Franzreb, Iris L Batalha, Ana Cecilia A Roque, Clemens Posten
Nov 29, 2017
BACKGROUND: In situ magnetic separation (ISMS) has emerged as a powerful tool to overcome process constraints such as product degradation or inhibition of target production. In the present work, an integrated ISMS process was...
Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models.
SPWD Vries, S Gupta, A Baig, E Wright, A Wedley, AN Jensen, LL Lora, S Humphrey, H Skovgård, K Macleod, E Pont, DP Wolanska, J L'Heureux, FM Mobegi, DGE Smith, P Everest, A Zomer, N Williams, P Wigley, T Humphrey, DJ Maskell, AJ Grant
Jun 01, 2017
Absorption of Nasal and Bronchial Fluids
Ryan S Thwaites, Hannah C Jarvis, Nehmat Singh, Akhilesh Jha, Andy Pritchard, Hailing Fan, Tanushree Tunstall, Joan Nanan, Simon Nadel, Onn Min Kon, Peter J Openshaw, Trevor T Hansel
Aug 28, 2019
The methods of nasal absorption (NA) and bronchial absorption (BA) use synthetic absorptive matrices (SAM) to absorb the mucosal lining fluid (MLF) of the human respiratory tract. NA is a non-invasive technique which absorbs...
Investigating the Campylobacter jejuni Transcriptional Response to Host Intestinal Extracts Reveals the Involvement of a Widely Conserved Iron Uptake System.
Martha M Liu, Christine J Boinett, Anson CK Chan, Julian Parkhill, Michael EP Murphy, Erin C Gaynor
Aug 29, 2019
Campylobacter jejuni is a pathogenic bacterium that causes gastroenteritis in humans yet is a widespread commensal in wild and domestic animals, particularly poultry. Using RNA sequencing, we assessed C. jejuni transcriptional...
Campylobacter jejuni RNA-seq acid resistance chicken cecal extract human fecal extract iron transport p19 streptomycin resistance Transcriptome Animals Campylobacter jejuni Cecum Chickens Complex Mixtures Culture Media Drug Resistance Bacterial Feces Gene Expression Regulation Bacterial Humans Iron Phenotype Sequence Analysis RNA Streptomycin Transcriptome
DNMTs Play an Important Role in Maintaining the Pluripotency of Leukemia Inhibitory Factor-Dependent Embryonic Stem Cells.
Baojiang Wu, Yunxia Li, Bojiang Li, Baojing Zhang, Yanqiu Wang, Lin Li, Junpeng Gao, Yuting Fu, Shudong Li, Chen Chen, M Azim Surani, Fuchou Tang, Xihe Li, Siqin Bao
Apr 16, 2021
Naive pluripotency can be maintained in medium with two inhibitors plus leukemia inhibitory factor (2i/LIF) supplementation, which primarily affects canonical WNT, FGF/ERK, and JAK/STAT3 signaling. However, whether one of these...
DNA Methylation DNMTs DIFFERENTIATION Embryonic Stem Cells epigenetic Genomic Imprinting Leukemia Inhibitory Factor MOUSE Pluripotency self-renew Animals Cell Culture Techniques Cell Differentiation Cell Self Renewal Cells Cultured Culture Media DNA (Cytosine-5-)-Methyltransferases DNA Methylation DNA Methyltransferase 3A Gene Expression Regulation Developmental Gene Knockout Techniques Genomic Imprinting Germ Layers Janus Kinases Leukemia Inhibitory Factor Mice Mice Inbred C57BL Mice Transgenic Mouse embryonic stem cells STAT3 Transcription Factor Signal Transduction Transcriptome
Essential Genes for In Vitro Growth of the Endophyte Herbaspirillum seropedicae SmR1 as Revealed by Transposon Insertion Site Sequencing.
Medulloblastoma Genotype Dictates Blood Brain Barrier Phenotype.
TN Phoenix, DM Patmore, S Boop, N Boulos, MO Jacus, YT Patel, MF Roussel, D Finkelstein, L Goumnerova, S Perreault, E Wadhwa, Y-J Cho, CF Stewart, RJ Gilbertson
Mar 11, 2016
Animals Antineoplastic Agents Blood-Brain Barrier Carrier Proteins Cerebellar Neoplasms Culture Media Conditioned Disease Models Animal Drug Resistance Neoplasm Endothelium Vascular Genetic Association Studies Genetic Vectors Genotype Glucose Transporter Type 1 Humans Medulloblastoma Membrane Proteins Mice Mice Transgenic Neoplasm Proteins Paracrine Communication Pericytes Recombinant Fusion Proteins Tight Junctions Transduction Genetic Vincristine Wnt Proteins Wnt Signaling Pathway
Pluripotency-associated genes in human nasopharyngeal carcinoma CNE-2 cells are reactivated by a unique epigenetic sub-microenvironment.
Jun-Xia Cao, Yu-Xin Cui, Zi-Jie Long, Zhong-Min Dai, Ji-Yan Lin, Yi Liang, Fei-Meng Zheng, Yi-Xin Zeng, Quentin Liu
Apr 16, 2012
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