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IP3 Receptors Preferentially Associate with ER-Lysosome Contact Sites and Selectively Deliver Ca2+ to Lysosomes.
Peace Atakpa, Nagendra Babu Thillaiappan, Stefania Mataragka, David L Prole, Colin W Taylor
Dec 21, 2018
Inositol 1,4,5-trisphosphate (IP3) receptors (IP3Rs) allow extracellular stimuli to redistribute Ca2+ from the ER to cytosol or other organelles. We show, using small interfering RNA (siRNA) and vacuolar H+-ATPase (V-ATPase)...
Ca(2+) IP(3) receptor concanamycin A Endoplasmic Reticulum genetically encoded Ca(2+) sensor lysosome membrane contact site proximity ligation assay store-operated Ca(2+) entry Calcium Calcium Signaling Cytosol Endoplasmic Reticulum HEK293 Cells HeLa Cells Humans Hydrogen-Ion Concentration Inositol 1 4 5-Trisphosphate Receptors Lysosomes
The role of tRNA synthetases in neurological and neuromuscular disorders.
Aminoacyl-tRNA synthetases (ARSs) are ubiquitously expressed enzymes responsible for charging tRNAs with their cognate amino acids, therefore essential for the first step in protein synthesis. Although the majority of protein...
Mutation of the cytosolic ribosomal protein-encoding RPS10B gene affects shoot meristematic function in Arabidopsis.
Arabidopsis Arabidopsis Proteins Cytosol DNA Complementary F-Box Proteins Gene Expression Regulation Plant Genes Plant Indoleacetic Acids Inflorescence Meristem Mutation Naphthaleneacetic Acids Phenotype Plant Leaves Plant Shoots Promoter Regions Genetic Protein Interaction Mapping Receptors Cell Surface Ribosomal Proteins Transcription Factors
Dysregulation of Mitochondrial Ca2+ Uptake and Sarcolemma Repair Underlie Muscle Weakness and Wasting in Patients and Mice Lacking MICU1.
Valentina Debattisti, Adam Horn, Raghavendra Singh, Erin L Seifert, Marshall W Hogarth, Davi A Mazala, Kai Ting Huang, Rita Horvath, Jyoti K Jaiswal, György Hajnóczky
Apr 21, 2020
Muscle function is regulated by Ca2+, which mediates excitation-contraction coupling, energy metabolism, adaptation to exercise, and sarcolemmal repair. Several of these actions rely on Ca2+ delivery to the mitochondrial matrix...
Atrophy autosomal recessive Calcium Injury membrane Mitochondria mitochondrial disease muscle disease Adolescent Adult Animals Calcium Calcium Signaling Calcium-Binding Proteins Cation Transport Proteins Cell Membrane Cytosol Female FIBROBLASTS Homeostasis Humans Male Mice Knockout Mitochondria Mitochondrial Membrane Transport Proteins Models Biological Muscle Contraction Muscle Weakness Muscle Skeletal Muscular Atrophy Sarcolemma Tetanus Wasting Syndrome
MAVS polymers smaller than 80 nm induce mitochondrial membrane remodeling and interferon signaling.
Ming-Shih Hwang, Jérôme Boulanger, Jonathan D Howe, Anna Albecka, Mathias Pasche, Leila Mureşan, Yorgo Modis
Feb 11, 2019
storm Cell Death innate immunity pathogen-associated molecular pattern Signal Transduction 3T3 Cells Adaptor Proteins Signal Transducing Animals Cell Death Cytosol FIBROBLASTS Interferon-Induced Helicase IFIH1 Interferon-beta Membrane Transport Proteins Mice Mice Knockout Microscopy Mitochondrial Membranes Mitochondrial Precursor Protein Import Complex Proteins Protein Domains RNA Double-Stranded Receptors Cell Surface Signal Transduction Single-Cell Analysis West Nile Fever
NBEAL2 is required for neutrophil and NK cell function and pathogen defense.
John M Sowerby, David C Thomas, Simon Clare, Marion Espéli, Jose A Guerrero, Kim Hoenderdos, Katherine Harcourt, Morgan Marsden, Juneid Abdul-Karim, Mathew Clement, Robin Antrobus, Yagnesh Umrania, Philippa R Barton, Shaun M Flint, Jatinder K Juss, Alison M Condliffe, Paul A Lyons, Ian R Humphreys, Edwin R Chilvers, Willem H Ouwehand, Gordon Dougan, Kenneth GC Smith
Aug 09, 2017
Mutations in the human NBEAL2 gene cause gray platelet syndrome (GPS), a bleeding diathesis characterized by a lack of α granules in platelets. The functions of the NBEAL2 protein have not been explored outside platelet biology...
A Conserved Mitochondrial ATP-binding Cassette Transporter Exports Glutathione Polysulfide for Cytosolic Metal Cofactor Assembly
ABC transporter Arabidopsis Glutathione Iron-Sulfur Protein Mitochondria Transportomics Yeast ATP-Binding Cassette Transporters Adenosine Triphosphatases Amino Acid Sequence Arabidopsis Arabidopsis Proteins Biological Transport Conserved Sequence Cytosol Glutathione Metals Mitochondrial Proteins Molecular Sequence Data Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sequence Homology Amino Acid Sulfides
Sustained signalling by PTH modulates IP3 accumulation and IP3 receptors through cyclic AMP junctions.
Ca2+ signalling Cyclic AMP Inositol trisphosphate receptor Parathyroid hormone Adenylyl Cyclases Calcium Calcium Signaling Carbachol Colforsin Cyclic AMP Cyclic AMP-Dependent Protein Kinases Cytosol HEK293 Cells Humans Inositol 1 4 5-Trisphosphate Inositol 1 4 5-Trisphosphate Receptors Parathyroid Hormone Receptor Parathyroid Hormone Type 1
Calcium Na(+)/K(+)-ATPase Platelet Protein Kinase C Sarco/endoplasmic reticulum Ca(2+)-ATPase Adenosine Diphosphate Blood Platelets Calcium Calcium Channels Calcium Signaling Cell Membrane Cytosol Humans Indoles Isoenzymes Platelet Activation Platelet Aggregation Inhibitors Protein Isoforms Protein Kinase C Thrombin
Viroplasms
Viroplasms are cytoplasmic, membraneless structures assembled in rotavirus (RV)-infected cells, which are intricately involved in viral replication. Two virus-encoded, non-structural proteins, NSP2 and NSP5, are the main drivers...
Crispr-Csy4 Genome Editing Nsp2 Nsp5 liquid-liquid phase separation Protein-Rna Condensates Replication Cycle Reverse Genetics Rotavirus Viroplasm Animals Capsid Proteins Cytoplasm Cytosol Humans Phosphorylation RNA-Binding Proteins Rotavirus Rotavirus Infections Viral Nonstructural Proteins Viral Replication Compartments Virus Assembly Virus Replication
Amino acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis.
Mark A Johnson, Sara Vidoni, Romina Durigon, Sarah F Pearce, Joanna Rorbach, Jiuya He, Gloria Brea-Calvo, Michal Minczuk, Aurelio Reyes, Ian J Holt, Antonella Spinazzola
Nov 13, 2017
Amino acids are essential for cell growth and proliferation for they can serve as precursors of protein synthesis, be remodelled for nucleotide and fat biosynthesis, or be burnt as fuel. Mitochondria are energy producing...
Membrane Tension Orchestrates Rear Retraction in Matrix-Directed Cell Migration.
Joseph HR Hetmanski, Henry de Belly, Ignacio Busnelli, Thomas Waring, Roshna V Nair, Vanesa Sokleva, Oana Dobre, Angus Cameron, Nils Gauthier, Christophe Lamaze, Joe Swift, Aránzazu Del Campo, Tobias Starborg, Tobias Zech, Jacky G Goetz, Ewa K Paluch, Jean-Marc Schwartz, Patrick T Caswell
Jul 02, 2020
In development, wound healing, and cancer metastasis, vertebrate cells move through 3D interstitial matrix, responding to chemical and physical guidance cues. Protrusion at the cell front has been extensively studied, but the...
RhoGTPase Caveolae cell invasion cell migration Cytoskeleton durotaxis Extracellular Matrix membrane tension Actin Cytoskeleton Actins Animals Caveolae Cell Line Tumor Cell Membrane Cell Movement Cell Polarity Cell Surface Extensions Cytoskeleton Cytosol Extracellular Matrix Humans Mice Protein Kinase C Pseudopodia Rats Signal Transduction rho-Associated Kinases rhoA GTP-Binding Protein
Two human metabolites rescue a C. elegans model of Alzheimer's disease via a cytosolic unfolded protein response.
Priyanka Joshi, Michele Perni, Ryan Limbocker, Benedetta Mannini, Sam Casford, Sean Chia, Johnny Habchi, Johnathan Labbadia, Christopher M Dobson, Michele Vendruscolo
Jun 03, 2021
Introduction of glucan synthase into the cytosol in wheat endosperm causes massive maltose accumulation and represses starch synthesis.
Tina B Schreier, Brendan Fahy, Laure C David, Hamad Siddiqui, Roger Castells-Graells, Alison M Smith
May 05, 2021
We expressed a bacterial glucan synthase (Agrobacterium GlgA) in the cytosol of developing endosperm cells in wheat grains, to discover whether it could generate a glucan from cytosolic ADP-glucose. Transgenic lines had high...
Identification of an RNA Polymerase III Regulator Linked to Disease-Associated Protein Aggregation.
Olga Sin, Tristan de Jong, Alejandro Mata-Cabana, Michelle Kudron, Mohamad Amr Zaini, Francesco A Aprile, Renée I Seinstra, Esther Stroo, Roméo Willinge Prins, Céline N Martineau, Hai Hui Wang, Wytse Hogewerf, Anne Steinhof, Erich E Wanker, Michele Vendruscolo, Cornelis F Calkhoven, Valerie Reinke, Victor Guryev, Ellen AA Nollen
Sep 09, 2019
Protein aggregation is associated with age-related neurodegenerative disorders, such as Alzheimer's and polyglutamine diseases. As a causal relationship between protein aggregation and neurodegeneration remains elusive...
C. elegans MOAG-2/LIR-3 RNA Polymerase III Non-coding RNA polyglutamine protein aggregation Protein homeostasis protein quality control snoRNA tRNA Active Transport Cell Nucleus Animals Animals Genetically Modified Binding Sites Caenorhabditis elegans Caenorhabditis elegans Proteins Cell Nucleus Cytosol Disease Models Animal Neurodegenerative Diseases Peptides Promoter Regions Genetic Protein Aggregates protein aggregation Pathological Protein Binding RNA Interference RNA Polymerase III RNA Small Untranslated Transcription Factors Transcription Genetic
Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.
Janice SW Ng, Maya A Hanspal, Naunehal S Matharu, Teresa P Barros, Elin K Esbjörner, Mark R Wilson, Justin J Yerbury, Christopher M Dobson, Janet R Kumita
Sep 09, 2019
TAR DNA-binding protein 43 (TDP-43) has been identified as the major constituent of the proteinaceous inclusions that are characteristic of most forms of amyotrophic lateral sclerosis (ALS) and ubiquitin positive frontotemporal...
Amino Acid Sequence Amyotrophic Lateral Sclerosis Cell Line Tumor Cell Nucleus Cysteine Cytosol DNA-Binding Proteins Electroporation Fluoresceins Fluorescent Dyes Frontotemporal Lobar Degeneration Humans Kinetics Models Biological Optical Imaging Organometallic Compounds Protein Transport Sequence Tagged Sites Time-Lapse Imaging Transfection
Fumarate induces vesicular release of mtDNA to drive innate immunity.
Vincent Zecchini, Vincent Paupe, Irene Herranz-Montoya, Joëlle Janssen, Inge MN Wortel, Jordan L Morris, Ashley Ferguson, Suvagata Roy Chowdury, Marc Segarra-Mondejar, Ana SH Costa, Gonçalo C Pereira, Laura Tronci, Timothy Young, Efterpi Nikitopoulou, Ming Yang, Dóra Bihary, Federico Caicci, Shun Nagashima, Alyson Speed, Kalliopi Bokea, Zara Baig, Shamith Samarajiwa, Maxine Tran, Thomas Mitchell, Mark Johnson, Julien Prudent, Christian Frezza
Mar 16, 2023
Reduced number of axonal mitochondria and tau hypophosphorylation in mouse P301L tau knockin neurons.
Teresa Rodríguez-Martín, Amy M Pooler, Dawn HW Lau, Gábor M Mórotz, Kurt J De Vos, Jonathan Gilley, Michael P Coleman, Diane P Hanger
Jun 18, 2018
Expression of the frontotemporal dementia-related tau mutation, P301L, at physiological levels in adult mouse brain (KI-P301L mice) results in overt hypophosphorylation of tau and age-dependent alterations in axonal...
Determination of the Global Pattern of Gene Expression in Yeast Cells by Intracellular Levels of Guanine Nucleotides.
Correlations between gene transcription and the abundance of high-energy purine nucleotides in Saccharomyces cerevisiae have often been noted. However, there has been no systematic investigation of this phenomenon in the absence...
Protein Condensation, Cellular Organization, and Spatiotemporal Regulation of Cytoplasmic Properties.
The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane.
Amino Acid Motifs Animals Cell Membrane Cytoplasm Cytosol Endoplasmic Reticulum Flow Cytometry Golgi Apparatus HEK293 Cells Humans Inflammation Ligands Microscopy Confocal Microscopy Fluorescence Mutagenesis Myeloid Differentiation Factor 88 Protein Structure Tertiary Signal Transduction Toll-Like Receptor 4 Vesicular Transport Proteins
Protein kinase A negatively regulates Ca2+ signalling in Toxoplasma gondii.
Alessandro D Uboldi, Mary-Louise Wilde, Emi A McRae, Rebecca J Stewart, Laura F Dagley, Luning Yang, Nicholas J Katris, Sanduni V Hapuarachchi, Michael J Coffey, Adele M Lehane, Cyrille Y Botte, Ross F Waller, Andrew I Webb, Malcolm J McConville, Christopher J Tonkin
Dec 13, 2018
The phylum Apicomplexa comprises a group of obligate intracellular parasites that alternate between intracellular replicating stages and actively motile extracellular forms that move through tissue. Parasite cytosolic Ca2+...
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