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Results: 127
A novel antibody combination to identify KIR2DS2high natural killer cells in KIR2DL3/L2/S2 heterozygous donors.
Matthew D Blunt, Pauline Rettman, Leidy Y Bastidas-Legarda, Rebecca Fulton, Valentina Capizzuto, Mohammed M Naiyer, James A Traherne, Salim I Khakoo
Dec 10, 2018
The killer cell immunoglobulin-like receptor (KIR) KIR2DS2 induces natural killer (NK) cell activation upon ligation and in genetic studies is associated with protection against certain cancers and viral infections. One of the...
Potency of Human Cardiosphere-Derived Cells from Patients with Ischemic Heart Disease Is Associated with Robust Vascular Supportive Ability.
Emma Harvey, Huajun Zhang, Pilar Sepúlveda, Sara P Garcia, Dominic Sweeney, Fizzah A Choudry, Delia Castellano, George N Thomas, Hassan Kattach, Romina Petersen, Derek J Blake, David P Taggart, Mattia Frontini, Suzanne M Watt, Enca Martin-Rendon
Oct 25, 2018
Cardiosphere-derived cell (CDC) infusion into damaged myocardium has shown some reparative effect; this could be improved by better selection of patients and cell subtype. CDCs isolated from patients with ischemic heart disease...
Flow cytometry for near-patient testing in premature neonates reveals variation in platelet function
Amie K Waller, Lajos Lantos, Audrienne Sammut, Burak Salgin, Harriet McKinney, Holly R Foster, Neline Kriek, Jonathan M Gibbins, Simon J Stanworth, Stephen F Garner, Vidheya Venkatesh, Anna Curley, Gusztav Belteki, Cedric Ghevaert
Feb 07, 2019
Genetic or pharmaceutical blockade of phosphoinositide 3-kinase p110δ prevents chronic rejection of heart allografts.
Huijun Ying, Hongmei Fu, Marlene L Rose, Ann M McCormack, Padmini Sarathchandra, Klaus Okkenhaug, Federica M Marelli-Berg
Jun 28, 2018
Chronic rejection is the major cause of long-term heart allograft failure, characterized by tissue infiltration by recipient T cells with indirect allospecificity. Phosphoinositol-3-kinase p110δ is a key mediator of T cell...
adenine Animals Cells Cultured Chronic Disease Class Ia Phosphatidylinositol 3-Kinase Female Flow Cytometry Graft Rejection H-Y Antigen Heart Transplantation Humans Immune Tolerance Male Mice Mice Inbred C57BL Mice Knockout Microscopy Fluorescence Phosphoinositide-3 Kinase Inhibitors Quinazolines Skin Transplantation T-Lymphocytes Transplantation Homologous
Autophagy regulates Notch degradation and modulates stem cell development and neurogenesis.
Fibroblast growth factor receptor 1 is principally responsible for fibroblast growth factor 2-induced catabolic activities in human articular chondrocytes.
Dongyao Yan, Di Chen, Simon M Cool, Andre J van Wijnen, Katalin Mikecz, Gillian Murphy, Hee-Jeong Im
Oct 12, 2011
Blotting Western Cartilage Articular Cell Proliferation Cell Separation Cells Cultured Chondrocytes Extracellular Signal-Regulated MAP Kinases Fibroblast Growth Factor 2 Flow Cytometry Humans Immunoprecipitation Osteoarthritis RNA Small Interfering Real-Time Polymerase Chain Reaction Receptor Fibroblast Growth Factor Type 1 Receptor Fibroblast Growth Factor Type 3 Signal Transduction Transfection
RNA
Rachel E Rigby, Lauren M Webb, Karen J Mackenzie, Yue Li, Andrea Leitch, Martin AM Reijns, Rachel J Lundie, Ailsa Revuelta, Donald J Davidson, Sandra Diebold, Yorgo Modis, Andrew S MacDonald, Andrew P Jackson
Sep 04, 2020
The sensing of nucleic acids by receptors of the innate immune system is a key component of antimicrobial immunity. RNA:DNA hybrids, as essential intracellular replication intermediates generated during infection, could...
Animals Blotting Western Dendritic Cells Endosomes Enzyme-Linked Immunosorbent Assay Flow Cytometry Fluorescence Polarization Fluorescent Antibody Technique Humans Immunity Innate Immunoblotting Mice Mice Inbred C57BL Models Immunological Myeloid Differentiation Factor 88 Nucleic Acid Heteroduplexes Real-Time Polymerase Chain Reaction Signal Transduction Toll-Like Receptor 9
Gut mucosa dissociation protocols influence cell type proportions and single-cell gene expression levels.
Werna TC Uniken Venema, Aarón D Ramírez-Sánchez, Emilia Bigaeva, Sebo Withoff, Iris Jonkers, Rebecca E McIntyre, Mennatallah Ghouraba, Tim Raine, Rinse K Weersma, Lude Franke, Eleonora AM Festen, Monique GP van der Wijst
Jul 15, 2022
Single-cell RNA sequencing (scRNA-seq) has revolutionized the study of the cellular landscape of organs. Most single-cell protocols require fresh material, which limits sample size per experiment, and consequently, introduces...
T lymphocyte senescence is attenuated in Parkinson's disease.
Antonina Kouli, Melanie Jensen, Vanesa Papastavrou, Kirsten M Scott, Claire Kolenda, Craig Parker, Imtiaz H Solim, Marta Camacho, Carmen Martin-Ruiz, Caroline H Williams-Gray
Oct 07, 2021
BACKGROUND: Immune involvement is well-described in Parkinson's disease (PD), including an adaptive T lymphocyte response. Given the increasing prevalence of Parkinson's disease in older age, age-related dysregulation of T...
Platelet Isolation and Activation Assays.
Platelets regulate hemostasis and are the key determinants of pathogenic thrombosis following atherosclerotic plaque rupture. Platelets circulate in an inactive state, but become activated in response to damage to the...
One in a million
Anastasia Zinchenko, Sean RA Devenish, Balint Kintses, Pierre-Yves Colin, Martin Fischlechner, Florian Hollfelder
Jan 22, 2021
Directed evolution relies on iterative cycles of randomization and selection. The outcome of an artificial evolution experiment is crucially dependent on (i) the numbers of variants that can be screened and (ii) the quality of...
Organoid Sample Preparation and Extraction for LC-MS Peptidomics.
Emily L Miedzybrodzka, Rachel E Foreman, Sam G Galvin, Pierre Larraufie, Amy L George, Deborah A Goldspink, Frank Reimann, Fiona M Gribble, Richard G Kay
Mar 18, 2021
This protocol describes the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry (LC-MS). Currently, most peptides are quantified by ELISA...
Suppression of Aβ toxicity by puromycin-sensitive aminopeptidase is independent of its proteolytic activity.
Antonina J Kruppa, Stanislav Ott, Dhia S Chandraratna, James A Irving, Richard M Page, Elena Speretta, Tiffany Seto, Luiz Miguel Camargo, Stefan J Marciniak, David A Lomas, Damian C Crowther
May 18, 2018
The accumulation of β-amyloid (Aβ) peptide in the brain is one of the pathological hallmarks of Alzheimer's disease and is thought to be of primary aetiological significance. In an unbiased genetic screen, we identified...
Alzheimer Amyloid Autophagy Proteolysis Puromycin-sensitive aminopeptidase Alzheimer Disease Aminopeptidases Amyloid beta-Peptides Animals Animals Genetically Modified Autophagy Blotting Western Brain Drosophila melanogaster Enzyme-Linked Immunosorbent Assay Flow Cytometry Fluorescent Antibody Technique Humans Immunoenzyme Techniques Neuroblastoma Neurons Proteolysis puromycin RNA Messenger Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Tumor Cells Cultured
IP3 3-kinase opposes NGF driven neurite outgrowth.
Richard Eva, Dalila Bouyoucef-Cherchalli, Kalpana Patel, Peter J Cullen, George Banting
Jun 29, 2018
The inositol (1,4,5) trisphosphate 3-kinases comprise a family of enzymes (A, B, and C) that phosphorylate the calcium mobilising molecule inositol (1,4,5) trisphosphate (IP(3)) to generate inositol (1,3,4,5) tetrakisphosphate....
Actins Animals Catalysis Cell Differentiation Cell Separation Extracellular Signal-Regulated MAP Kinases Flow Cytometry Green Fluorescent Proteins Hippocampus Models Biological Nerve Growth Factor Neurites Neurons PC12 Cells Phenotype Phosphotransferases (Alcohol Group Acceptor) Rats Signal Transduction
Application of phage display to high throughput antibody generation and characterization.
Darren J Schofield, Anthony R Pope, Veronica Clementel, Jenny Buckell, Susan Dj Chapple, Kay F Clarke, Jennie S Conquer, Anna M Crofts, Sandra RE Crowther, Michael R Dyson, Gillian Flack, Gareth J Griffin, Yvette Hooks, William J Howat, Anja Kolb-Kokocinski, Susan Kunze, Cecile D Martin, Gareth L Maslen, Joanne N Mitchell, Maureen O'Sullivan, Rajika L Perera, Wendy Roake, S Paul Shadbolt, Karen J Vincent, Anthony Warford, Wendy E Wilson, Jane Xie, Joyce L Young, John McCafferty
Jun 17, 2011
CD146 expression is associated with a poor prognosis in human breast tumors and with enhanced motility in breast cancer cell lines.
Gwladys Zabouo, Anne-Marie Imbert, Jocelyne Jacquemier, Pascal Finetti, Thomas Moreau, Benjamin Esterni, Daniel Birnbaum, François Bertucci, Christian Chabannon
Jun 13, 2011
Adult Aged Aged 80 and over Breast Neoplasms CD146 Antigen Cell Movement Female Flow Cytometry Gene Expression Profiling Gene Expression Regulation Neoplastic Gene Silencing Humans Immunoenzyme Techniques Mesoderm Middle Aged Oligonucleotide Array Sequence Analysis Prognosis RNA Messenger RNA Small Interfering Reverse Transcriptase Polymerase Chain Reaction Tissue Array Analysis Tumor Cells Cultured Young Adult
Pulmonary retention of primed neutrophils
Charlotte Summers, Nanak R Singh, Jessica F White, Iain M Mackenzie, Andrew Johnston, Chandra Solanki, KK Balan, A Michael Peters, Edwin R Chilvers
Aug 14, 2014
ARDS Neutrophil Biology Blood Flow Velocity Cell Movement Erythrocytes Female Flow Cytometry Granulocyte-Macrophage Colony-Stimulating Factor Humans Indium Radioisotopes Male Middle Aged Neutrophils Platelet Activating Factor Radionuclide Imaging Respiratory Distress Syndrome Spirometry Technetium Time Factors
Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells.
Dharini Iyer, Laure Gambardella, William G Bernard, Felipe Serrano, Victoria L Mascetti, Roger A Pedersen, Amarnath Talasila, Sanjay Sinha
May 18, 2015
High-throughput detection of ethanol-producing cyanobacteria in a microdroplet platform.
Peripheral Blood Cell-Stratified Subgroups of Inflamed Depression.
Mary-Ellen Lynall, Lorinda Turner, Junaid Bhatti, Jonathan Cavanagh, Peter de Boer, Valeria Mondelli, Declan Jones, Wayne C Drevets, Philip Cowen, Neil A Harrison, Carmine M Pariante, Linda Pointon, Menna R Clatworthy, Edward Bullmore, Neuroimmunology of Mood Disorders and Alzheimer’s Disease (NIMA) Consortium
Dec 11, 2019
Indole at low concentration helps exponentially growing Escherichia coli survive at high temperature.
A culture of stationary phase Escherichia coli cells has been reported to produce copious indole when exposed to high temperature (50°C), and this response has been proposed to aid survival. We reinvestigated this phenomenon and...
Mesenchyme-derived IGF2 is a major paracrine regulator of pancreatic growth and function.
Constanze M Hammerle, Ionel Sandovici, Gemma V Brierley, Nicola M Smith, Warren E Zimmer, Ilona Zvetkova, Haydn M Prosser, Yoichi Sekita, Brian YH Lam, Marcella Ma, Wendy N Cooper, Antonio Vidal-Puig, Susan E Ozanne, Gema Medina-Gómez, Miguel Constância
Aug 24, 2020
Single-cell genomics for resolution of conserved bacterial genes and mobile genetic elements of the human intestinal microbiota using flow cytometry.
Dylan Lawrence, Danielle E Campbell, Lawrence A Schriefer, Rachel Rodgers, Forrest C Walker, Marissa Turkin, Lindsay Droit, Miles Parkes, Scott A Handley, Megan T Baldridge
Mar 13, 2022
As our understanding of the importance of the human microbiota in health and disease grows, so does our need to carefully resolve and delineate its genomic content. 16S rRNA gene-based analyses yield important insights into...
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