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The AP-1-BATF and -BATF3 module is essential for growth, survival and TH17/ILC3 skewing of anaplastic large cell lymphoma.
Nikolai Schleussner, Olaf Merkel, Mariantonia Costanza, Huan-Chang Liang, Franziska Hummel, Chiara Romagnani, Pawel Durek, Ioannis Anagnostopoulos, Michael Hummel, Korinna Jöhrens, Antonia Niedobitek, Patrick R Griffin, Roberto Piva, Henrike L Sczakiel, Wilhelm Woessmann, Christine Damm-Welk, Christian Hinze, Dagmar Stoiber, Bernd Gillissen, Suzanne D Turner, Eva Kaergel, Linda von Hoff, Michael Grau, Georg Lenz, Bernd Dörken, Claus Scheidereit, Lukas Kenner, Martin Janz, Stephan Mathas
Mar 20, 2018
Transcription factor AP-1 is constitutively activated and IRF4 drives growth and survival in ALK+ and ALK- anaplastic large cell lymphoma (ALCL). Here we demonstrate high-level BATF and BATF3 expression in ALCL. Both BATFs bind...
Basic-Leucine Zipper Transcription Factors Binding Sites CRISPR-Cas Systems Carrier Proteins Cell Death Cell Line Tumor Cell Survival Cytokines Gene Editing Gene Expression Regulation Neoplastic Gene Knockdown Techniques Humans Lymphoma Large-Cell Anaplastic Protein Binding Protein Kinase Inhibitors RNA Small Interfering T-Lymphocyte Subsets Th17 Cells Transcription Factor AP-1 Transcriptome
The implications of the gender-based prohibitions relating to human germline genome editing in the Human Fertilisation and Embryology Act.
Amarpreet Kaur
Nov 16, 2020
Assisted Reproductive Technologies Gender recognition certificates Germline genome editing Legislation transgender Uterine Transplants Adolescent Adult Aged Aged 80 and over embryology Female Gender Identity Gene Editing Germ Cells Humans Male Middle Aged Surveys and Questionnaires Transgender Persons Young Adult
Rapid genome editing by CRISPR-Cas9-POLD3 fusion.
Ganna Reint, Zhuokun Li, Kornel Labun, Salla Keskitalo, Inkeri Soppa, Katariina Mamia, Eero Tolo, Monika Szymanska, Leonardo A Meza-Zepeda, Susanne Lorenz, Artur Cieslar-Pobuda, Xian Hu, Diana L Bordin, Judith Staerk, Eivind Valen, Bernhard Schmierer, Markku Varjosalo, Jussi Taipale, Emma Haapaniemi
Jan 28, 2022
Gene Correction Recovers Phagocytosis in Retinal Pigment Epithelium Derived from Retinitis Pigmentosa-Human-Induced Pluripotent Stem Cells.
Ana Artero-Castro, Kathleen Long, Andrew Bassett, Almudena Ávila-Fernandez, Marta Cortón, Antonio Vidal-Puig, Pavla Jendelova, Francisco Javier Rodriguez-Jimenez, Eleonora Clemente, Carmen Ayuso, Slaven Erceg
Feb 21, 2021
Hereditary retinal dystrophies (HRD) represent a significant cause of blindness, affecting mostly retinal pigment epithelium (RPE) and photoreceptors (PRs), and currently suffer from a lack of effective treatments. Highly...
RPE Retinitis Pigmentosa gene correction Induced Pluripotent Stem Cells Cell Differentiation Cell Line Gene Editing Gene Expression Regulation Humans Induced Pluripotent Stem Cells Mutation Phagocytosis Retinal Photoreceptor Cell Outer Segment Retinal Pigment Epithelium Retinitis Pigmentosa c-Mer Tyrosine Kinase
Organoids of the female reproductive tract.
Cancers Female reproductive tract Fertility Infection Organoids Pregnancy Reproductive Health Animals CRISPR-Cas Systems Drug Discovery Estrous Cycle Extracellular Matrix Female Fertility Gene Editing Genital Diseases female Genitalia female Gestational Age Gonadal Steroid Hormones Humans Maternal-Fetal Exchange Mice Organoids Pituitary Hormones Anterior Placenta Precision Medicine Pregnancy Stem Cells Tissue Engineering
Frequent loss of heterozygosity in CRISPR-Cas9-edited early human embryos.
Gregorio Alanis-Lobato, Jasmin Zohren, Afshan McCarthy, Norah ME Fogarty, Nada Kubikova, Emily Hardman, Maria Greco, Dagan Wells, James MA Turner, Kathy K Niakan
Jun 07, 2021
CRISPR-Cas9 genome editing is a promising technique for clinical applications, such as the correction of disease-associated alleles in somatic cells. The use of this approach has also been discussed in the context of heritable...
In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering.
Qianxin Wu, Quentin RV Ferry, Toni A Baeumler, Yale S Michaels, Dimitrios M Vitsios, Omer Habib, Roland Arnold, Xiaowei Jiang, Stefano Maio, Bruno R Steinkraus, Marta Tapia, Paolo Piazza, Ni Xu, Georg A Holländer, Thomas A Milne, Jin-Soo Kim, Anton J Enright, Andrew R Bassett, Tudor A Fulga
May 01, 2018
RNA regulatory elements (RREs) are an important yet relatively under-explored facet of gene regulation. Deciphering the prevalence and functional impact of this post-transcriptional control layer requires technologies for...
Genome editing in mitochondria corrects a pathogenic mtDNA mutation in vivo.
Payam A Gammage, Carlo Viscomi, Marie-Lune Simard, Ana SH Costa, Edoardo Gaude, Christopher A Powell, Lindsey Van Haute, Beverly J McCann, Pedro Rebelo-Guiomar, Raffaele Cerutti, Lei Zhang, Edward J Rebar, Massimo Zeviani, Christian Frezza, James B Stewart, Michal Minczuk
Nov 05, 2018
Mutations of the mitochondrial genome (mtDNA) underlie a substantial portion of mitochondrial disease burden. These disorders are currently incurable and effectively untreatable, with heterogeneous penetrance, presentation and...
Linear mitochondrial DNA is rapidly degraded by components of the replication machinery.
Viktoriya Peeva, Daniel Blei, Genevieve Trombly, Sarah Corsi, Maciej J Szukszto, Pedro Rebelo-Guiomar, Payam A Gammage, Alexei P Kudin, Christian Becker, Janine Altmüller, Michal Minczuk, Gábor Zsurka, Wolfram S Kunz
Jul 17, 2018
Emerging gene therapy approaches that aim to eliminate pathogenic mutations of mitochondrial DNA (mtDNA) rely on efficient degradation of linearized mtDNA, but the enzymatic machinery performing this task is presently unknown....
Base Sequence CRISPR-Cas Systems DNA Breaks Double-Stranded DNA Cleavage DNA Helicases DNA Polymerase gamma DNA Replication DNA Mitochondrial Deoxyribonucleases Type II Site-Specific Electron Transport Complex IV Exodeoxyribonucleases Gene Editing Genetic Therapy HEK293 Cells Humans Mitochondria Recombinant Fusion Proteins
A transposable element insertion is associated with an alternative life history strategy.
Alyssa Woronik, Kalle Tunström, Michael W Perry, Ramprasad Neethiraj, Constanti Stefanescu, Maria de la Paz Celorio-Mancera, Oskar Brattström, Jason Hill, Philipp Lehmann, Reijo Käkelä, Christopher W Wheat
Dec 22, 2020
Animals Butterflies CRISPR-Cas Systems Color DNA Transposable Elements Female Gene Editing Gene Expression Profiling Gene Expression Regulation Developmental Genetic Loci Homeodomain Proteins Life History Traits Male Pigmentation Pigments Biological Reproduction Sex Factors whole genome sequencing Wings Animal
The Origins and Vulnerabilities of Two Transmissible Cancers in Tasmanian Devils.
Maximilian R Stammnitz, Tim HH Coorens, Kevin C Gori, Dane Hayes, Beiyuan Fu, Jinhong Wang, Daniel E Martin-Herranz, Ludmil B Alexandrov, Adrian Baez-Ortega, Syd Barthorpe, Alexandra Beck, Francesca Giordano, Graeme W Knowles, Young Mi Kwon, George Hall, Stacey Price, Ruth J Pye, Jose MC Tubio, Hannah VT Siddle, Sukhwinder Singh Sohal, Gregory M Woods, Ultan McDermott, Fengtang Yang, Mathew J Garnett, Zemin Ning, Elizabeth P Murchison
Mar 13, 2018
Transmissible cancers are clonal lineages that spread through populations via contagious cancer cells. Although rare in nature, two facial tumor clones affect Tasmanian devils. Here we perform comparative genetic and functional...
DFTD Tasmanian devils Cancer Cancer evolution Cancer genomics CONSERVATION contagious cancer drug screening marsupials transmissible cancer Animals Cell Line Tumor Chromosomes Mammalian Clone Cells Facial Neoplasms Female Gene Dosage Gene Editing Immunity Male Marsupialia Mutation Receptors Platelet-Derived Growth Factor
Adaptation of Plasmodium falciparum to humans involved the loss of an ape-specific erythrocyte invasion ligand.
William R Proto, Sasha V Siegel, Selasi Dankwa, Weimin Liu, Alison Kemp, Sarah Marsden, Zenon A Zenonos, Steve Unwin, Paul M Sharp, Gavin J Wright, Beatrice H Hahn, Manoj T Duraisingh, Julian C Rayner
Nov 26, 2019
Plasmodium species are frequently host-specific, but little is currently known about the molecular factors restricting host switching. This is particularly relevant for P. falciparum, the only known human-infective species of...
Translational adaptation to heat stress is mediated by RNA 5-methylcytosine in Caenorhabditis elegans.
Isabela Cunha Navarro, Francesca Tuorto, David Jordan, Carine Legrand, Jonathan Price, Fabian Braukmann, Alan G Hendrick, Alper Akay, Annika Kotter, Mark Helm, Frank Lyko, Eric A Miska
Nov 12, 2020
Methylation of carbon-5 of cytosines (m5 C) is a post-transcriptional nucleotide modification of RNA found in all kingdoms of life. While individual m5 C-methyltransferases have been studied, the impact of the global cytosine-5...
Caenorhabditis elegans 5-Methylcytosine NSUN RNA modifications translation efficiency 5-Methylcytosine Adaptation Physiological Animals CRISPR-Cas Systems Caenorhabditis elegans Cytosine Gene Editing Heat-Shock Response Hot Temperature Leucine Methyltransferases Mitochondria Proline Protein Biosynthesis RNA RNA processing Post-Transcriptional Ribosomes
IL10RA modulates crizotinib sensitivity in NPM1-ALK+ anaplastic large cell lymphoma.
Nina Prokoph, Nicola A Probst, Liam C Lee, Jack M Monahan, Jamie D Matthews, Huan-Chang Liang, Klaas Bahnsen, Ivonne A Montes-Mojarro, Elif Karaca-Atabay, Geeta G Sharma, Vikas Malik, Hugo Larose, Sorcha D Forde, Stephen P Ducray, Cosimo Lobello, Qi Wang, Shi-Lu Luan, Šárka Pospíšilová, Carlo Gambacorti-Passerini, GA Amos Burke, Shahid Pervez, Andishe Attarbaschi, Andrea Janíková, Hélène Pacquement, Judith Landman-Parker, Anne Lambilliotte, Gudrun Schleiermacher, Wolfram Klapper, Ralf Jauch, Wilhelm Woessmann, Gilles Vassal, Lukas Kenner, Olaf Merkel, Luca Mologni, Roberto Chiarle, Laurence Brugières, Birgit Geoerger, Isaia Barbieri, Suzanne D Turner
May 26, 2020
Anaplastic large cell lymphoma (ALCL) is a T-cell malignancy predominantly driven by a hyperactive anaplastic lymphoma kinase (ALK) fusion protein. ALK inhibitors, such as crizotinib, provide alternatives to standard...
Antineoplastic Agents CRISPR-Cas Systems Cell Line Crizotinib Dose-Response Relationship Drug Drug Resistance Neoplasm Gene Editing Gene Expression Humans Immunohistochemistry Interleukin-10 Receptor alpha Subunit Lymphoma Large-Cell Anaplastic Models Biological Nucleophosmin Protein Kinase Inhibitors Protein-Tyrosine Kinases STAT3 Transcription Factor Signal Transduction
Enhancing the genome editing toolbox
Emmanouil Metzakopian, Alex Strong, Vivek Iyer, Alex Hodgkins, Konstantinos Tzelepis, Liliana Antunes, Mathias J Friedrich, Qiaohua Kang, Teresa Davidson, Jacob Lamberth, Christina Hoffmann, Gregory D Davis, George S Vassiliou, William C Skarnes, Allan Bradley
Oct 14, 2020
CRISPR-Cas9 technology has accelerated biological research becoming routine for many laboratories. It is rapidly replacing conventional gene editing techniques and has high utility for both genome-wide and gene-focussed...
Allele specific repair of splicing mutations in cystic fibrosis through AsCas12a genome editing.
Giulia Maule, Antonio Casini, Claudia Montagna, Anabela S Ramalho, Kris De Boeck, Zeger Debyser, Marianne S Carlon, Gianluca Petris, Anna Cereseto
Oct 23, 2020
One-step generation of conditional and reversible gene knockouts
A Andersson-Rolf, RC Mustata, A Merenda, J Kim, S Perera, T Grego, K Andrews, K Tremble, J Rebelo da Silva, J Fink, WC Skarnes, B-K Koo
Mar 13, 2017
High molecular weight DNA assembly in vivo for synthetic biology applications.
mRNA processing in mutant zebrafish lines generated by chemical and CRISPR-mediated mutagenesis produces unexpected transcripts that escape nonsense-mediated decay.
Jennifer L Anderson, Timothy S Mulligan, Meng-Chieh Shen, Hui Wang, Catherine M Scahill, Frederick J Tan, Shao J Du, Elisabeth M Busch-Nentwich, Steven A Farber
Jan 24, 2018
As model organism-based research shifts from forward to reverse genetics approaches, largely due to the ease of genome editing technology, a low frequency of abnormal phenotypes is being observed in lines with mutations...
CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing.
Ting-Wei Will Chiang, Carlos le Sage, Delphine Larrieu, Mukerrem Demir, Stephen P Jackson
Apr 13, 2016
Alleles Base Sequence CRISPR-Cas Systems Cell Line Deoxyribonuclease I Drug Discovery Gene Editing Gene Knockout Techniques Gene Order Gene Targeting Genetic Loci Genetic Vectors Genomics Genotype High-Throughput Screening Assays Humans Mutagenesis Phenotype RNA Guide Kinetoplastida Vascular Endothelial Growth Factor A
CRISPR-Cas9 correction of OPA1 c.1334G>A
Paul E Sladen, Pedro RL Perdigão, Grace Salsbury, Tatiana Novoselova, Jacqueline van der Spuy, J Paul Chapple, Patrick Yu-Wai-Man, Michael E Cheetham
Nov 01, 2021
Autosomal dominant optic atrophy (DOA) is the most common inherited optic neuropathy in the United Kingdom. DOA has an insidious onset in early childhood, typically presenting with bilateral, central visual loss caused by the...
Rapid genome editing by CRISPR-Cas9-POLD3 fusion
Ganna Reint, Zhuokun Li, Kornel Labun, Salla Keskitalo, Inkeri Soppa, Katariina Mamia, Eero Tolo, Monika Szymanska, Leonardo A Meza-Zepeda, Susanne Lorenz, Artur Cieslar-Pobuda, Xian Hu, Diana L Bordin, Judith Staerk, Eivind Valen, Bernhard Schmierer, Markku Varjosalo, Jussi Taipale, Emma Haapaniemi
Jan 28, 2022
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