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Results: 61
A computational platform for high-throughput analysis of RNA sequences and modifications by mass spectrometry.
Samuel Wein, Byron Andrews, Timo Sachsenberg, Helena Santos-Rosa, Oliver Kohlbacher, Tony Kouzarides, Benjamin A Garcia, Hendrik Weisser
Apr 14, 2020
The field of epitranscriptomics continues to reveal how post-transcriptional modification of RNA affects a wide variety of biological phenomena. A pivotal challenge in this area is the identification of modified RNA residues...
Evaluation of FTIR spectroscopy as a diagnostic tool for lung cancer using sputum.
Paul D Lewis, Keir E Lewis, Robin Ghosal, Sion Bayliss, Amanda J Lloyd, John Wills, Ruth Godfrey, Philip Kloer, Luis AJ Mur
May 13, 2020
BACKGROUND: Survival time for lung cancer is poor with over 90% of patients dying within five years of diagnosis primarily due to detection at late stage. The main objective of this study was to evaluate Fourier transform...
Variant rs10911021 that associates with coronary heart disease in type 2 diabetes, is associated with lower concentrations of circulating HDL cholesterol and large HDL particles but not with amino acids.
Katherine E Beaney, Jackie A Cooper, Stela McLachlan, S Goya Wannamethee, Barbara J Jefferis, Peter Whincup, Yoav Ben-Shlomo, Jacqueline F Price, Meena Kumari, Andrew Wong, Ken Ong, Rebecca Hardy, Diana Kuh, Mika Kivimaki, Antti J Kangas, Pasi Soininen, Mika Ala-Korpela, Fotios Drenos, Steve E Humphries, UCLEB Consortium
Sep 29, 2016
AIMS: An intergenic locus on chromosome 1 (lead SNP rs10911021) was previously associated with coronary heart disease (CHD) in type 2 diabetes (T2D). Using data from the UCLEB consortium we investigated the relationship between...
coronary heart disease genetic risk HDL-cholesterol Metabolomics Aged Amino Acids Biomarkers Cholesterol HDL Chromosomes Human Pair 1 Coronary Disease DNA Intergenic Diabetes mellitus Type 2 Down-Regulation Female Gene Frequency Genetic Association Studies Genetic Predisposition to Disease High-Throughput Screening Assays Humans Magnetic Resonance Spectroscopy Male Metabolomics Middle Aged Particle Size Phenotype Polymorphism Single Nucleotide Protective factors Risk Assessment Risk Factors
A Biobank of Breast Cancer Explants with Preserved Intra-tumor Heterogeneity to Screen Anticancer Compounds.
Alejandra Bruna, Oscar M Rueda, Wendy Greenwood, Ankita Sati Batra, Maurizio Callari, Rajbir Nath Batra, Katherine Pogrebniak, Jose Sandoval, John W Cassidy, Ana Tufegdzic-Vidakovic, Stephen-John Sammut, Linda Jones, Elena Provenzano, Richard Baird, Peter Eirew, James Hadfield, Matthew Eldridge, Anne McLaren-Douglas, Andrew Barthorpe, Howard Lightfoot, Mark J O'Connor, Joe Gray, Javier Cortes, Jose Baselga, Elisabetta Marangoni, Alana L Welm, Samuel Aparicio, Violeta Serra, Mathew J Garnett, Carlos Caldas
Oct 21, 2016
Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi.
Ming Wang, Martin Schäfer, Dapeng Li, Rayko Halitschke, Chuanfu Dong, Erica McGale, Christian Paetz, Yuanyuan Song, Suhua Li, Junfu Dong, Sven Heiling, Karin Groten, Philipp Franken, Michael Bitterlich, Maria J Harrison, Uta Paszkowski, Ian T Baldwin
Dec 13, 2018
High-through-put (HTP) screening for functional arbuscular mycorrhizal fungi (AMF)-associations is challenging because roots must be excavated and colonization evaluated by transcript analysis or microscopy. Here we show that...
Nicotiana attenuata Rhizophagus irregularis arbuscular mycorrhizal fungi blumenol colonization rate Ecology high-throughput screening plant biology Biomarkers Cyclohexanones Genes Plant High-Throughput Screening Assays Metabolomics Mycorrhizae Plant Leaves Plant Shoots RNA Messenger Stress Physiological Symbiosis Time Factors Tobacco
Genome-wide high-throughput screening to investigate essential genes involved in methicillin-resistant Staphylococcus aureus Sequence Type 398 survival.
Mette T Christiansen, Rolf S Kaas, Roy R Chaudhuri, Mark A Holmes, Henrik Hasman, Frank M Aarestrup
Jul 15, 2014
Animals Anti-Bacterial Agents DNA Transposable Elements Gene Library Genes Essential Genome Genotype High-Throughput Screening Assays Humans Hydrogen-Ion Concentration Methicillin-Resistant Staphylococcus aureus Mutation Plasmids Sequence Analysis DNA Staphylococcal Infections Swine Virulence Factors
High-throughput detection of ethanol-producing cyanobacteria in a microdroplet platform.
Industrial scale high-throughput screening delivers multiple fast acting macrofilaricides.
Rachel H Clare, Catherine Bardelle, Paul Harper, W David Hong, Ulf Börjesson, Kelly L Johnston, Matthew Collier, Laura Myhill, Andrew Cassidy, Darren Plant, Helen Plant, Roger Clark, Darren AN Cook, Andrew Steven, John Archer, Paul McGillan, Sitthivut Charoensutthivarakul, Jaclyn Bibby, Raman Sharma, Gemma L Nixon, Barton E Slatko, Lindsey Cantin, Bo Wu, Joseph Turner, Louise Ford, Kirsty Rich, Mark Wigglesworth, Neil G Berry, Paul M O'Neill, Mark J Taylor, Stephen A Ward
Feb 25, 2019
Nematodes causing lymphatic filariasis and onchocerciasis rely on their bacterial endosymbiont, Wolbachia, for survival and fecundity, making Wolbachia a promising therapeutic target. Here we perform a high-throughput screen of...
Identification of genetic elements in metabolism by high-throughput mouse phenotyping.
Jan Rozman, Birgit Rathkolb, Manuela A Oestereicher, Christine Schütt, Aakash Chavan Ravindranath, Stefanie Leuchtenberger, Sapna Sharma, Martin Kistler, Monja Willershäuser, Robert Brommage, Terrence F Meehan, Jeremy Mason, Hamed Haselimashhadi, IMPC Consortium, Tertius Hough, Ann-Marie Mallon, Sara Wells, Luis Santos, Christopher J Lelliott, Jacqueline K White, Tania Sorg, Marie-France Champy, Lynette R Bower, Corey L Reynolds, Ann M Flenniken, Stephen A Murray, Lauryl MJ Nutter, Karen L Svenson, David West, Glauco P Tocchini-Valentini, Arthur L Beaudet, Fatima Bosch, Robert B Braun, Michael S Dobbie, Xiang Gao, Yann Herault, Ala Moshiri, Bret A Moore, KC Kent Lloyd, Colin McKerlie, Hiroshi Masuya, Nobuhiko Tanaka, Paul Flicek, Helen E Parkinson, Radislav Sedlacek, Je Kyung Seong, Chi-Kuang Leo Wang, Mark Moore, Steve D Brown, Matthias H Tschöp, Wolfgang Wurst, Martin Klingenspor, Eckhard Wolf, Johannes Beckers, Fausto Machicao, Andreas Peter, Harald Staiger, Hans-Ulrich Häring, Harald Grallert, Monica Campillos, Holger Maier, Helmut Fuchs, Valerie Gailus-Durner, Thomas Werner, Martin Hrabe de Angelis
Oct 14, 2020
Metabolic diseases are a worldwide problem but the underlying genetic factors and their relevance to metabolic disease remain incompletely understood. Genome-wide research is needed to characterize so-far unannotated mammalian...
High-throughput elucidation of thrombus formation reveals sources of platelet function variability.
Johanna P van Geffen, Sanne LN Brouns, Joana Batista, Harriet McKinney, Carly Kempster, Magdolna Nagy, Suthesh Sivapalaratnam, Constance CFMJ Baaten, Nikki Bourry, Mattia Frontini, Kerstin Jurk, Manuela Krause, Daniele Pillitteri, Frauke Swieringa, Remco Verdoold, Rachel Cavill, Marijke JE Kuijpers, Willem H Ouwehand, Kate Downes, Johan WM Heemskerk
Oct 31, 2019
In combination with microspotting, whole-blood microfluidics can provide high-throughput information on multiple platelet functions in thrombus formation. Based on assessment of the inter- and intra-subject variability in...
Extracting fluorescent reporter time courses of cell lineages from high-throughput microscopy at low temporal resolution
Mike J Downey, Danuta M Jeziorska, Sascha Ott, T Katherine Tamai, Georgy Koentges, Keith W Vance, Till Bretschneider
Jan 01, 0001
The extraction of fluorescence time course data is a major bottleneck in high-throughput live-cell microscopy. Here we present an extendible framework based on the open-source image analysis software ImageJ, which aims in...
Structure-guided fragment-based drug discovery at the synchrotron
Sherine E Thomas, Patrick Collins, Rory Hennell James, Vitor Mendes, Sitthivut Charoensutthivarakul, Chris Radoux, Chris Abell, Anthony G Coyne, R Andres Floto, Frank von Delft, Tom L Blundell
Apr 03, 2019
Mycobacterium abscessus SAICAR synthetase (PurC) Fragment-based drug discovery Structure-guided synchrotron Bacterial Proteins Binding Sites Drug Discovery High-Throughput Screening Assays History 20th Century History 21st Century Humans Models Molecular Mycobacterium abscessus Peptide Fragments Peptide Synthases Synchrotrons
High-throughput discovery of novel developmental phenotypes.
Mary E Dickinson, Ann M Flenniken, Xiao Ji, Lydia Teboul, Michael D Wong, Jacqueline K White, Terrence F Meehan, Wolfgang J Weninger, Henrik Westerberg, Hibret Adissu, Candice N Baker, Lynette Bower, James M Brown, L Brianna Caddle, Francesco Chiani, Dave Clary, James Cleak, Mark J Daly, James M Denegre, Brendan Doe, Mary E Dolan, Sarah M Edie, Helmut Fuchs, Valerie Gailus-Durner, Antonella Galli, Alessia Gambadoro, Juan Gallegos, Shiying Guo, Neil R Horner, Chih-Wei Hsu, Sara J Johnson, Sowmya Kalaga, Lance C Keith, Louise Lanoue, Thomas N Lawson, Monkol Lek, Manuel Mark, Susan Marschall, Jeremy Mason, Melissa L McElwee, Susan Newbigging, Lauryl MJ Nutter, Kevin A Peterson, Ramiro Ramirez-Solis, Douglas J Rowland, Edward Ryder, Kaitlin E Samocha, John R Seavitt, Mohammed Selloum, Zsombor Szoke-Kovacs, Masaru Tamura, Amanda G Trainor, Ilinca Tudose, Shigeharu Wakana, Jonathan Warren, Olivia Wendling, David B West, Leeyean Wong, Atsushi Yoshiki, International Mouse Phenotyping Consortium, Jackson Laboratory, Institut Clinique de la Souris (ICS) Infrastructure Nationale PHENOMIN, Charles River Laboratories, MRC Harwell, Toronto Centre for Phenogenomics, Wellcome Trust Sanger Institute, RIKEN BioResource Center, Daniel G MacArthur, Glauco P Tocchini-Valentini, Xiang Gao, Paul Flicek, Allan Bradley, William C Skarnes, Monica J Justice, Helen E Parkinson, Mark Moore, Sara Wells, Robert E Braun, Karen L Svenson, Martin Hrabe de Angelis, Yann Herault, Tim Mohun, Ann-Marie Mallon, R Mark Henkelman, Steve DM Brown, David J Adams, KC Kent Lloyd, Colin McKerlie, Arthur L Beaudet, Maja Bućan, Stephen A Murray
Oct 22, 2020
Approximately one-third of all mammalian genes are essential for life. Phenotypes resulting from knockouts of these genes in mice have provided tremendous insight into gene function and congenital disorders. As part of the...
Ultrahigh-Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay.
Miriam Klaus, Paul Jannis Zurek, Tomasz S Kaminski, Ahir Pushpanath, Katharina Neufeld, Florian Hollfelder
Oct 13, 2021
Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput.
Compartmentalization of single genes in water-in-oil emulsion droplets is a powerful approach to create millions of reactors for enzyme library selections. When these droplets are formed at ultrahigh throughput in microfluidic...
cell-free protein synthesis directed evolution Microfluidics Protein Engineering ultrahigh throughput Bacillus Bacterial Proteins Base Sequence DNA Bacterial Directed Molecular Evolution Emulsions Escherichia coli Genes Bacterial High-Throughput Screening Assays Microfluidic Analytical Techniques Microfluidics Nucleic Acid Amplification Techniques Plasmids Protein Engineering Serine Endopeptidases Workflow
Cell-based screen for altered nuclear phenotypes reveals senescence progression in polyploid cells after Aurora kinase B inhibition.
Mahito Sadaie, Christian Dillon, Masako Narita, Andrew RJ Young, Claire J Cairney, Lauren S Godwin, Christopher J Torrance, Dorothy C Bennett, W Nicol Keith, Masashi Narita
Jun 30, 2015
A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division.
Lovorka Stojic, Aaron TL Lun, Patrice Mascalchi, Christina Ernst, Aisling M Redmond, Jasmin Mangei, Alexis R Barr, Vicky Bousgouni, Chris Bakal, John C Marioni, Duncan T Odom, Fanni Gergely
Feb 11, 2020
Genome stability relies on proper coordination of mitosis and cytokinesis, where dynamic microtubules capture and faithfully segregate chromosomes into daughter cells. With a high-content RNAi imaging screen targeting more than...
Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins.
Laurens Lindenburg, Tuomas Huovinen, Kayleigh van de Wiel, Michael Herger, Michael R Snaith, Florian Hollfelder
Apr 21, 2020
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