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Results: 14
Human Stem Cell-Derived Endothelial-Hepatic Platform for Efficacy Testing of Vascular-Protective Metabolites from Nutraceuticals.
Balakrishnan Chakrapani Narmada, Yeek Teck Goh, Huan Li, Sanjay Sinha, Hanry Yu, Christine Cheung
Feb 07, 2019
Atherosclerosis underlies many cardiovascular and cerebrovascular diseases. Nutraceuticals are emerging as a therapeutic moiety for restoring vascular health. Unlike small-molecule drugs, the complexity of ingredients in...
Coculture Endothelial Cells Hepatocytes Human pluripotent stem cells metabolism Nutraceutical testing Culture Media Conditioned Dietary Supplements endothelial progenitor cells Genistein Hepatocytes Human Embryonic Stem Cells Humans Inflammation Interleukin-1beta Liver Metabolome Pluripotent Stem Cells Protective Agents Quercetin
Optimized inducible shRNA and CRISPR/Cas9 platforms for $\textit{in vitro}$ studies of human development using hPSCs
A Bertero, M Pawlowski, D Ortmann, K Snijders, L Yiangou, M Cardoso de Brito, S Brown, WG Bernard, JD Cooper, E Giacomelli, L Gambardella, NRF Hannan, D Iyer, F Sampaziotis, F Serrano, MCF Zonneveld, S Sinha, M Kotter, L Vallier
Dec 14, 2016
Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells.
Dharini Iyer, Laure Gambardella, William G Bernard, Felipe Serrano, Victoria L Mascetti, Roger A Pedersen, Amarnath Talasila, Sanjay Sinha
May 18, 2015
Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells.
Leqian Yu, Junjun Li, Jiayin Hong, Yasuhiro Takashima, Nanae Fujimoto, Minako Nakajima, Akihisa Yamamoto, Xiaofeng Dong, Yujiao Dang, Yu Hou, Wei Yang, Itsunari Minami, Keisuke Okita, Motomu Tanaka, Chunxiong Luo, Fuchou Tang, Yong Chen, Chao Tang, Hidetoshi Kotera, Li Liu
Mar 18, 2021
We show that a human pluripotent stem cell (hPSC) population cultured on a low-adhesion substrate developed two hPSC subtypes with different colony morphologies: flat and domed. Notably, the dome-like cells showed higher active...
cell-matrix adhesion Heterogeneity Human pluripotent stem cells mathematical model nanofiber Serum Response Factor single-cell culture stem cell morphology Biomarkers Cell Adhesion Cell Differentiation Cell Proliferation Cell Self Renewal Cell-Matrix Junctions Gene Expression Humans Immunophenotyping Karyotype Kruppel-Like Factor 4 Models Biological Pluripotent Stem Cells
A Novel Human Pluripotent Stem Cell-Derived Neural Crest Model of Treacher Collins Syndrome Shows Defects in Cell Death and Migration.
Felipe Serrano, William George Bernard, Alessandra Granata, Dharini Iyer, Ben Steventon, Matthew Kim, Ludovic Vallier, Laure Gambardella, Sanjay Sinha
Dec 10, 2018
The neural crest (NC) is a transient multipotent cell population present during embryonic development. The NC can give rise to multiple cell types and is involved in a number of different diseases. Therefore, the development of...
Treacher Collins syndrome DIFFERENTIATION Disease Modeling Human pluripotent stem cells Neural Crest Adaptor Proteins Signal Transducing Animals Benzamides Cell Death Cell Differentiation Cell Movement Cellular Reprogramming Techniques Chick Embryo Dioxoles Fibroblast Growth Factor 2 Humans Mandibulofacial Dysostosis Neural Crest Neural Stem Cells Nuclear Proteins Phosphoproteins Pluripotent Stem Cells SOXE Transcription Factors Transcription Factor AP-2 Transcription Factors Twist-Related Protein 1
Isolation and Comparative Transcriptome Analysis of Human Fetal and iPSC-Derived Cone Photoreceptor Cells.
Emily Welby, Jorn Lakowski, Valentina Di Foggia, Dimitri Budinger, Anai Gonzalez-Cordero, Aaron TL Lun, Michael Epstein, Aara Patel, Elisa Cuevas, Kamil Kruczek, Arifa Naeem, Federico Minneci, Mike Hubank, David T Jones, John C Marioni, Robin R Ali, Jane C Sowden
May 23, 2018
Loss of cone photoreceptors, crucial for daylight vision, has the greatest impact on sight in retinal degeneration. Transplantation of stem cell-derived L/M-opsin cones, which form 90% of the human cone population, could provide...
cell surface markers cell transplantation therapy cone photoreceptor cells Human pluripotent stem cells Retinal Dystrophies retinal organoids Transcriptome Cell Differentiation Fetus Gene Expression Profiling Gene Expression Regulation Developmental Humans Induced Pluripotent Stem Cells Retina Retinal Cone Photoreceptor Cells Retinal Degeneration Rod Opsins Transcriptome
Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency.
Nicholas Bredenkamp, Jian Yang, James Clarke, Giuliano Giuseppe Stirparo, Ferdinand von Meyenn, Sabine Dietmann, Duncan Baker, Rosalind Drummond, Yongming Ren, Dongwei Li, Chuman Wu, Maria Rostovskaya, Sarah Eminli-Meissner, Austin Smith, Ge Guo
Oct 16, 2019
RNA-mediated reprogramming Wnt signaling Human pluripotent stem cells human pre-implantation epiblast Induced Pluripotent Stem Cells molecular reprogramming naive pluripotency Biomarkers Cellular Reprogramming FIBROBLASTS Gene Expression Profiling Humans Induced Pluripotent Stem Cells RNA RNA Messenger Reproducibility of Results Signal Transduction Wnt Proteins
Metabolomics Identifies Metabolic Markers of Maturation in Human Pluripotent Stem Cell-Derived Cardiomyocytes.
Vijesh J Bhute, Xiaoping Bao, Kaitlin K Dunn, Kylie R Knutson, Eric C McCurry, Gyuhyung Jin, Wei-Hua Lee, Sarah Lewis, Akihiro Ikeda, Sean P Palecek
Jun 20, 2018
Cardiovascular disease is a leading cause of death worldwide. Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) hold immense clinical potential and recent studies have enabled generation of virtually pure hPSC-CMs...
Human pluripotent stem cell-derived epicardial progenitors can differentiate to endocardial-like endothelial cells.
Xiaoping Bao, Vijesh J Bhute, Tianxiao Han, Tongcheng Qian, Xiaojun Lian, Sean P Palecek
Jun 20, 2018
During heart development, epicardial progenitors contribute various cardiac lineages including smooth muscle cells, cardiac fibroblasts, and endothelial cells. However, their specific contribution to the human endothelium has...
Synthetic scaffolds help airway cells reach maturity
Transplanting bioengineered human lung organoids into mice could lead to a humanized model for pre-clinical studies of lung disease.
Human Pluripotent Stem Cell-Derived Endoderm for Modeling Development and Clinical Applications.
The liver, lung, pancreas, and digestive tract all originate from the endoderm germ layer, and these vital organs are subject to many life-threatening diseases affecting millions of patients. However, primary cells from...
Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis.
Miller Huang, Jignesh Tailor, Qiqi Zhen, Aaron H Gillmor, Matthew L Miller, Holger Weishaupt, Justin Chen, Tina Zheng, Emily K Nash, Lauren K McHenry, Zhenyi An, Fubaiyang Ye, Yasuhiro Takashima, James Clarke, Harold Ayetey, Florence MG Cavalli, Betty Luu, Branden S Moriarity, Shirin Ilkhanizadeh, Lukas Chavez, Chunying Yu, Kathreena M Kurian, Thierry Magnaldo, Nicolas Sevenet, Philipp Koch, Steven M Pollard, Peter Dirks, Michael P Snyder, David A Largaespada, Yoon Jae Cho, Joanna J Phillips, Fredrik J Swartling, A Sorana Morrissy, Marcel Kool, Stefan M Pfister, Michael D Taylor, Austin Smith, William A Weiss
Jul 25, 2019
Human neural stem cell cultures provide progenitor cells that are potential cells of origin for brain cancers. However, the extent to which genetic predisposition to tumor formation can be faithfully captured in stem cell lines...
SHH Human pluripotent stem cells Medulloblastoma neuroepithelial stem cells Animals Basal Cell Nevus Syndrome Brain Neoplasms Carcinogenesis DEAD-box RNA Helicases Disease Models Animal Genetic Engineering Genetic Predisposition to Disease Humans Medulloblastoma Mice Mice SCID N-Myc Proto-Oncogene Protein Neoplasm Proteins Neural Stem Cells Neuroepithelial Cells Patched-1 Receptor Pluripotent Stem Cells Stem Cell Transplantation Transplantation Heterologous
GATA6 Cooperates with EOMES/SMAD2/3 to Deploy the Gene Regulatory Network Governing Human Definitive Endoderm and Pancreas Formation.
Crystal Y Chia, Pedro Madrigal, Simon LIJ Denil, Iker Martinez, Jose Garcia-Bernardo, Ranna El-Khairi, Mariya Chhatriwala, Maggie H Shepherd, Andrew T Hattersley, N Ray Dunn, Ludovic Vallier
Jan 28, 2019
GATA6 definitive endoderm Disease Modeling Human pluripotent stem cells pancreatic agenesis Cell Differentiation Cell Lineage Cells Cultured Endoderm GATA6 Transcription Factor Gene Regulatory Networks Homeodomain Proteins Humans Insulin-Secreting Cells Pancreas Pancreatic Diseases Pluripotent Stem Cells Protein Binding Smad2 Protein Smad3 Protein T-Box Domain Proteins Trans-Activators