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Mutant Z α1-antitrypsin (E342K) accumulates as polymers within the endoplasmic reticulum (ER) of hepatocytes predisposing to liver disease, whereas low levels of circulating Z α1-antitrypsin lead to emphysema by loss of...

Liver Disease Monoclonal antibody scFv intrabody serpin polymer Animals Base Sequence COS Cells Chlorocebus aethiops Endoplasmic Reticulum Humans Immunoblotting Intracellular Space Kinetics Leukocyte Elastase Mice Inbred BALB C Molecular Sequence Data Mutation Polymerization Protease Inhibitors Single-Chain Antibodies alpha 1-Antitrypsin

Viscosity sensitive fluorophores termed 'molecular rotors' represent a convenient and quantitative tool for measuring intracellular viscosity via Fluorescence Lifetime Imaging Microscopy (FLIM). We compare the FLIM performance...

Animals Biosensing Techniques Boron Compounds COS Cells Chlorocebus aethiops Intracellular Space Ligands LIGHT Microscopy Fluorescence Molecular Dynamics Simulation Protein Conformation Proteins Viscosity

Adaptation Physiological Biocatalysis Computational Biology evolution Molecular Glycolysis Intracellular Space Kinetics Models Biological Saccharomyces cerevisiae Temperature

We have investigated the relationship between the stability and secreted yield of a series of mutational variants of human lysozyme (HuL) in Pichia pastoris. We show that genes directly involved in the unfolded protein response...

Autophagy Endoplasmic Reticulum-Associated Degradation Enzyme-Linked Immunosorbent Assay Fungal Proteins Gene Expression Regulation Fungal Humans Intracellular Space Muramidase Mutant Proteins Pichia Protein Stability RNA Messenger Single-Chain Antibodies Unfolded Protein Response

Severe malaria is primarily caused by Plasmodium falciparum parasites during their asexual reproduction cycle within red blood cells. One of the least understood stages in this cycle is the brief preinvasion period during which...

Aniline Compounds Calcium Erythrocytes Formaldehyde Humans Intracellular Space Plasmodium falciparum Pyruvic Acid Xanthenes

Animals Cell Fractionation Immunohistochemistry Intracellular Space Machine Learning Mass Spectrometry Mice Mouse embryonic stem cells Multivariate Analysis Pluripotent Stem Cells Proteome Proteomics Subcellular Fractions

The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell...

3D correlative light and electron microscopy Autophagy Entosis HIV-1 Mycobacterium tuberculosis Serial blockface SEM Cell Survival Cells Cultured Endothelial Cells Entosis HIV Humans Imaging Three-Dimensional Intracellular Space MACROPHAGES Microscopy Electron Scanning Monocytes Mycobacterium tuberculosis

Staphylococcus aureus is a major human pathogen causing multiple pathologies, from cutaneous lesions to life-threatening sepsis. Although neutrophils contribute to immunity against S. aureus, multiple lines of evidence suggest...

Autophagy NADPH oxidase ROS Staphylococcus aureus lc3-associated phagocytosis (LAP) neutrophil Zebrafish Animals Animals Genetically Modified Autophagy Intracellular Space Kinetics MACROPHAGES Microtubule-Associated Proteins NADPH Oxidases Neutrophils Phagocytosis Phagosomes Protein Aggregates Sequestosome-1 Protein Staphylococcus aureus Zebrafish Zebrafish Proteins

Animals Bile Acids and Salts Calcium Cells Cultured Cyclic AMP Enteroendocrine Cells Glucagon-Like Peptide 1 Intestine Small Intracellular Space Male Mice Knockout Mice Transgenic Microscopy Fluorescence Rats Wistar Receptors G-Protein-Coupled Taurodeoxycholic Acid Taurolithocholic Acid Tissue Culture Techniques

The assembly of biomolecules into condensates is a fundamental process underlying the organisation of the intracellular space and the regulation of many cellular functions. Mapping and characterising phase behaviour of...

biomolecular condensates Intracellular Space Microfluidics Phase Transition

The genus Legionella comprises 65 species, among which Legionella pneumophila is a human pathogen causing severe pneumonia. To understand the evolution of an environmental to an accidental human pathogen, we have functionally...

Legionella coevolution horizontal gene transfer human pathogen protozoa Bacterial Proteins Bacterial Secretion Systems Computational Biology evolution Molecular Genome Bacterial Genomics Humans Intracellular Space Legionella Legionellosis Phylogeny Protein Domains

Reactive oxygen species (ROS) are produced continuously throughout the cell as products of various redox reactions. Yet these products function as important signal messengers, acting through oxidation of specific target factors....

antioxidants Dementia Endoplasmic Reticulum Hydrogen Peroxide neurodegeneration Reactive Oxygen Species redox signalling Animals Endoplasmic Reticulum Humans Hydrogen Peroxide Intracellular Space Nerve Degeneration Oxidative Stress Unfolded Protein Response

Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. Granzyme K (GrK) has been only limitedly characterized and knowledge on its molecular functions is emerging....

cytotoxic cells Cytotoxicity Disease granzyme K Inflammation Animals Bacterial Infections Cytokines Cytotoxicity Immunologic Endothelial Cells Extracellular Space Granzymes Humans Intracellular Space Lung Diseases Molecular Targeted Therapy Serine Proteinase Inhibitors Treatment Outcome Virus Diseases

assembly biogenesis glycine receptor human hyperekplexia rescue of function subcompartimentalization Amino Acid Sequence Animals COS Cells Child Chlorocebus aethiops Endoplasmic Reticulum Female Golgi Apparatus HEK293 Cells Humans Infant Intracellular Space Male Mice Molecular Sequence Data Neurons Pedigree Protein Structure Secondary Protein Structure Tertiary Receptors Glycine Stiff-Person Syndrome