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Results: 57
Single-cell Hi-C reveals cell-to-cell variability in chromosome structure.
Takashi Nagano, Yaniv Lubling, Tim J Stevens, Stefan Schoenfelder, Eitan Yaffe, Wendy Dean, Ernest D Laue, Amos Tanay, Peter Fraser
Jul 15, 2019
Large-scale chromosome structure and spatial nuclear arrangement have been linked to control of gene expression and DNA replication and repair. Genomic techniques based on chromosome conformation capture (3C) assess contacts for...
Mechanism of eIF6 release from the nascent 60S ribosomal subunit.
Félix Weis, Emmanuel Giudice, Mark Churcher, Li Jin, Christine Hilcenko, Chi C Wong, David Traynor, Robert R Kay, Alan J Warren
Sep 18, 2015
Cryoelectron Microscopy Dictyostelium Eukaryotic Initiation Factors GTP Phosphohydrolases Humans Models Biological Models Molecular Molecular Conformation Peptide Chain Initiation Translational Peptide Elongation Factors Proteins Protozoan Proteins Ribonucleoprotein U5 Small Nuclear Ribosome Subunits Large Eukaryotic
SSBP1-Disease Update
Neringa Jurkute, Fabiana D'Esposito, Anthony G Robson, Robert DS Pitceathly, Francesca Cordeiro, F Lucy Raymond, Anthony T Moore, Michel Michaelides, Patrick Yu-Wai-Man, Andrew R Webster, Gavin Arno, Genomics England Research Consortium
Jan 28, 2022
Adolescent Amino Acid Sequence Child Preschool DNA Mitochondrial DNA-Binding Proteins Electroretinography Female Genes Recessive Genotyping Techniques Humans Male Middle Aged Mitochondrial Diseases Mitochondrial Proteins Molecular Conformation Molecular Sequence Data Mutation Missense Optic Atrophy Pedigree Penetrance Protein Stability Protein Structure Quaternary Retinal Dystrophies whole genome sequencing
Expeditious Total Synthesis of Hemiasterlin through a Convergent Multicomponent Strategy and Its Use in Targeted Cancer Therapeutics.
Hemiasterlin is an antimitotic marine natural product with reported sub-nanomolar potency against several cancer cell lines. Herein, we describe an expeditious total synthesis of hemiasterlin featuring a four-component Ugi...
Two distinct conformational states define the interaction of human RAD51-ATP with single-stranded DNA.
Ineke Brouwer, Tommaso Moschetti, Andrea Candelli, Edwige B Garcin, Mauro Modesti, Luca Pellegrini, Gijs Jl Wuite, Erwin Jg Peterman
Sep 20, 2018
An essential mechanism for repairing DNA double-strand breaks is homologous recombination (HR). One of its core catalysts is human RAD51 (hRAD51), which assembles as a helical nucleoprotein filament on single-stranded DNA...
DNA Repair RAD51 homologous recombination single‐stranded DNA Adenosine Triphosphate Crystallography X-Ray DNA DNA Breaks Double-Stranded DNA Repair DNA Replication DNA Single-Stranded DNA-Binding Proteins homologous recombination Models Molecular Molecular Conformation Nucleoproteins Rad51 Recombinase
The most reactive amide as a transition-state mimic for cis-trans interconversion.
Igor V Komarov, Stanislav Yanik, Aleksandr Yu Ishchenko, John E Davies, Jonathan M Goodman, Anthony J Kirby
Mar 10, 2015
Cross-Module Enoylreduction in the Azalomycin F Polyketide Synthase.
Guifa Zhai, Wenyan Wang, Wei Xu, Guo Sun, Chaoqun Hu, Xiangming Wu, Zisong Cong, Liang Deng, Yanrong Shi, Peter F Leadlay, Heng Song, Kui Hong, Zixin Deng, Yuhui Sun
Oct 19, 2020
The colinearity of canonical modular polyketide synthases, which creates a direct link between multienzyme structure and the chemical structure of the biosynthetic end-product, has become a cornerstone of knowledge-based genome...
Structure of F1-ATPase from the obligate anaerobe Fusobacterium nucleatum.
Jessica Petri, Yoshio Nakatani, Martin G Montgomery, Scott A Ferguson, David Aragão, Andrew GW Leslie, Adam Heikal, John E Walker, Gregory M Cook
Jun 10, 2019
The crystal structure of the F1-catalytic domain of the adenosine triphosphate (ATP) synthase has been determined from the pathogenic anaerobic bacterium Fusobacterium nucleatum. The enzyme can hydrolyse ATP but is partially...
3D structure of individual mammalian genomes studied by single cell Hi-C
TJ Stevens, D Lando, S Basu, LP Atkinson, Y Cao, SF Lee, M Leeb, KJ Wohlfahrt, W Boucher, A O’Shaughnessy-Kirwan, J Cramard, AJ Faure, M Ralser, E Blanco, L Morey, M Sanso, MGS Palayret, B Lehner, L Di Croce, A Wutz, B Hendrich, D Klenerman, ED Laue
Mar 06, 2017
Animals CCCTC-Binding Factor Cell Cycle Proteins Chromatin Assembly and Disassembly Chromosomal Proteins Non-Histone Chromosomes Mammalian DNA Enhancer Elements Genetic G1 Phase Gene Expression Regulation Gene Regulatory Networks Genome Haploidy Mi-2 Nucleosome Remodeling and Deacetylase Complex Mice Models Molecular Molecular Conformation Molecular Imaging Mouse embryonic stem cells Nucleosomes Promoter Regions Genetic Repressor Proteins Reproducibility of Results Single-Cell Analysis
Total synthesis and biological evaluation of simplified aplyronine analogues as synthetically tractable anticancer agents.
Talia R Pettigrew, Rachel J Porter, Stephen J Walsh, Michael P Housden, Nelson YS Lam, Jason S Carroll, Jeremy S Parker, David R Spring, Ian Paterson
Jan 08, 2020
The aplyronines are a family of highly cytotoxic marine natural products with potential application in targeted cancer chemotherapy. To address the severe supply issue, function-oriented molecular editing of their macrolactone...
Systematic Evaluation of ReaxFF Reactive Force Fields for Biochemical Applications.
Four established ReaxFF force fields, trained on biochemical systems, have been systematically benchmarked on 20 proteinogenic amino acids and 11 dipeptides. The force fields were compared with respect to geometries, energetics...
Dimers of DNA-PK create a stage for DNA double-strand break repair.
Amanda K Chaplin, Steven W Hardwick, Shikang Liang, Antonia Kefala Stavridi, Ales Hnizda, Lee R Cooper, Taiana Maia De Oliveira, Dimitri Y Chirgadze, Tom L Blundell
Aug 25, 2020
Direct observation of heterogeneous amyloid fibril growth kinetics via two-color super-resolution microscopy.
Dorothea Pinotsi, Alexander K Buell, Celine Galvagnion, Christopher M Dobson, Gabriele S Kaminski Schierle, Clemens F Kaminski
Feb 12, 2020
The self-assembly of normally soluble proteins into fibrillar amyloid structures is associated with a range of neurodegenerative disorders, such as Parkinson's and Alzheimer's diseases. In the present study, we show that...
Macromolecular structure determination using X-rays, neutrons and electrons
Dorothee Liebschner, Pavel V Afonine, Matthew L Baker, Gábor Bunkóczi, Vincent B Chen, Tristan I Croll, Bradley Hintze, Li Wei Hung, Swati Jain, Airlie J McCoy, Nigel W Moriarty, Robert D Oeffner, Billy K Poon, Michael G Prisant, Randy J Read, Jane S Richardson, David C Richardson, Massimo D Sammito, Oleg V Sobolev, Duncan H Stockwell, Thomas C Terwilliger, Alexandre G Urzhumtsev, Lizbeth L Videau, Christopher J Williams, Paul D Adams
Aug 19, 2019
Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological processes and to develop new...
Sequential detection of multiple phase transitions in model biological membranes using a red-emitting conjugated polyelectrolyte.
The anionic conjugated polyelectrolyte, poly[3-(6-sulfothioatehexyl)thiophene] (P3Anionic), functions as a highly sensitive probe of membrane order, uniquely capable of sequentially detecting the three key phase transitions...
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