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Results: 104
Accurate mitochondrial DNA sequencing using off-target reads provides a single test to identify pathogenic point mutations.
Helen R Griffin, Angela Pyle, Emma L Blakely, Charlotte L Alston, Jennifer Duff, Gavin Hudson, Rita Horvath, Ian J Wilson, Mauro Santibanez-Koref, Robert W Taylor, Patrick F Chinnery
Mar 07, 2019
PURPOSE: Mitochondrial disorders are a common cause of inherited metabolic disease and can be due to mutations affecting mitochondrial DNA or nuclear DNA. The current diagnostic approach involves the targeted resequencing of...
Absence of xenotropic murine leukaemia virus-related virus in UK patients with chronic fatigue syndrome.
Harriet CT Groom, Virginie C Boucherit, Kerry Makinson, Edward Randal, Sarah Baptista, Suzanne Hagan, John W Gow, Frank M Mattes, Judith Breuer, Jonathan R Kerr, Jonathan P Stoye, Kate N Bishop
Mar 21, 2019
BACKGROUND: Detection of a retrovirus, xenotropic murine leukaemia virus-related virus (XMRV), has recently been reported in 67% of patients with chronic fatigue syndrome. We have studied a total of 170 samples from chronic...
Circulating tumor DNA in patients with colorectal adenomas
Ni Ni Moe Myint, Ajay M Verma, Daniel Fernandez-Garcia, Panchali Sarmah, Patrick S Tarpey, Saif Sattar Al-Aqbi, Hong Cai, Ricky Trigg, Kevin West, Lynne M Howells, Anne Thomas, Karen Brown, David S Guttery, Baljit Singh, Howard J Pringle, Ultan McDermott, Jacqui A Shaw, Alessandro Rufini
Nov 22, 2018
Improving early detection of colorectal cancer (CRC) is a key public health priority as adenomas and stage I cancer can be treated with minimally invasive procedures. Population screening strategies based on detection of occult...
Adenoma Adenomatous Polyposis Coli Protein Aged Aged 80 and over Animals Biomarkers Tumor Circulating Tumor DNA Colonic Neoplasms Early Detection of Cancer Female High-Throughput Nucleotide Sequencing Humans Liquid biopsy Male Mice Mice Knockout Middle Aged Polymerase Chain Reaction Proto-Oncogene Proteins B-raf Proto-Oncogene Proteins p21(ras)
Loss of skeletal muscle HIF-1alpha results in altered exercise endurance.
Steven D Mason, Richard A Howlett, Matthew J Kim, I Mark Olfert, Michael C Hogan, Wayne McNulty, Reed P Hickey, Peter D Wagner, C Ronald Kahn, Frank J Giordano, Randall S Johnson
Mar 13, 2020
The physiological flux of oxygen is extreme in exercising skeletal muscle. Hypoxia is thus a critical parameter in muscle function, influencing production of ATP, utilization of energy-producing substrates, and manufacture of...
Adenosine Triphosphate Alleles Animals creatine kinase Crosses Genetic Gene Deletion Gene Expression Regulation Genotype Glucose Glycogen Glycolysis Hematocrit Hemoglobins Hypoxia Hypoxia-Inducible Factor 1 alpha Subunit Lactic Acid Male Mice Mice Inbred C57BL Mice Knockout Microscopy Electron Muscle Skeletal Oxygen Physical Conditioning Animal Physical Exertion Polymerase Chain Reaction Promoter Regions Genetic Reverse Transcriptase Polymerase Chain Reaction
Differential gene expression profile in the small intestines of mice lacking pacemaker interstitial cells of Cajal.
Yataro Daigo, Ichiro Takayama, Bruce AJ Ponder, Carlos Caldas, Sean M Ward, Kenton M Sanders, Masayuki A Fujino
Jun 16, 2011
Norovirus translation requires an interaction between the C Terminus of the genome-linked viral protein VPg and eukaryotic translation initiation factor 4G.
Liliane Chung, Dalan Bailey, Eoin N Leen, Edward P Emmott, Yasmin Chaudhry, Lisa O Roberts, Stephen Curry, Nicolas Locker, Ian G Goodfellow
Jul 09, 2014
Eukaryotic Translation Initiation Factor 4E (eIF4E) Norovirus Plus-stranded RNA Virus Translation Translation Initiation Factor VPg Virus eIF4G Base Sequence Chromatography Affinity DNA Primers Eukaryotic Initiation Factor-4G Genome Viral Norovirus Polymerase Chain Reaction Protein Binding Protein Biosynthesis Proteomics Viral Proteins
Germline TERT promoter mutations are rare in familial melanoma.
Mark Harland, Mia Petljak, Carla Daniela Robles-Espinoza, Zhihao Ding, Nelleke A Gruis, Remco van Doorn, Karen A Pooley, Alison M Dunning, Lauren G Aoude, Karin AW Wadt, Anne-Marie Gerdes, Kevin M Brown, Nicholas K Hayward, Julia A Newton-Bishop, David J Adams, D Timothy Bishop
Oct 07, 2015
Strategies for sample labelling and library preparation in DNA metabarcoding studies.
Kristine Bohmann, Vasco Elbrecht, Christian Carøe, Iliana Bista, Florian Leese, Michael Bunce, Douglas W Yu, Mathew Seymour, Alex J Dumbrell, Simon Creer
Oct 13, 2021
Metabarcoding of DNA extracted from environmental or bulk specimen samples is increasingly used to profile biota in basic and applied biodiversity research because of its targeted nature that allows sequencing of genetic markers...
Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR.
Nubia Macedo, Marcelo Gottschalk, Katrin Strutzberg-Minder, Chao Nguyen Van, Lijun Zhang, Geng Zou, Rui Zhou, Thaire Marostica, Maria Jose Clavijo, Alexander Tucker, Virginia Aragon
Jun 13, 2021
Glaesserella parasuis strains were characterized by serotyping PCR, vtaA virulence marker Leader Sequence (LS)-PCR, clinical significance, and geographic region. Overall, the serovars 4, 5/12, 7, 1, and 13 were the most commonly...
Leptospira spp. Prevalence in Cats from Southern Italy with Evaluation of Risk Factors for Exposure and Clinical Findings in Infected Cats.
Giulia Donato, Marisa Masucci, Katrin Hartmann, Marga GA Goris, Ahmed A Ahmed, Joy Archer, Angela Alibrandi, Maria Grazia Pennisi
Nov 27, 2022
Leptospirosis is a worldwide zoonotic disease, but feline leptospirosis is rarely reported. This study aimed at investigating Leptospira spp. prevalence in cats from southern Italy, evaluating risk factors, clinical findings and...
Mutations in Mll2, an H3K4 methyltransferase, result in insulin resistance and impaired glucose tolerance in mice.
Michelle Goldsworthy, Nathan L Absalom, David Schröter, Helen C Matthews, Debora Bogani, Lee Moir, Anna Long, Christopher Church, Alison Hugill, Quentin M Anstee, Rob Goldin, Mark Thursz, Florian Hollfelder, Roger D Cox
Jan 22, 2021
We employed a random mutagenesis approach to identify novel monogenic determinants of type 2 diabetes. Here we show that haplo-insufficiency of the histone methyltransferase myeloid-lineage leukemia (Mll2/Wbp7) gene causes type...
A new PCR assay for the detection and differentiation of Babesia canis and Babesia vogeli.
Giada Annoscia, Maria Stefania Latrofa, Cinzia Cantacessi, Emanuela Olivieri, Maria Teresa Manfredi, Filipe Dantas-Torres, Domenico Otranto
Nov 28, 2017
Babesia spp. are globally distributed tick-borne protozoan parasites that infect the red blood cells of a wide range of vertebrate hosts, including humans. Diagnosis of babesiosis is often impeded by the transient presence of...
Analytical validation of a next generation sequencing liquid biopsy assay for high sensitivity broad molecular profiling.
Vincent Plagnol, Samuel Woodhouse, Karen Howarth, Stefanie Lensing, Matt Smith, Michael Epstein, Mikidache Madi, Sarah Smalley, Catherine Leroy, Jonathan Hinton, Frank de Kievit, Esther Musgrave-Brown, Colin Herd, Katherine Baker-Neblett, Will Brennan, Peter Dimitrov, Nathan Campbell, Clive Morris, Nitzan Rosenfeld, James Clark, Davina Gale, Jamie Platt, John Calaway, Greg Jones, Tim Forshew
Jul 16, 2018
Circulating tumor DNA (ctDNA) analysis is being incorporated into cancer care; notably in profiling patients to guide treatment decisions. Responses to targeted therapies have been observed in patients with actionable mutations...
Viral metagenomics of aphids present in bean and maize plots on mixed-use farms in Kenya reveals the presence of three dicistroviruses including a novel Big Sioux River virus-like dicistrovirus
JP Carr, FO Wamonje, GN Michuki, LA Braidwood, JN Njuguna, JM Mutuku, A Djikeng, JJW Harvey
Oct 03, 2017
High prevalence of HPV in non-cervical sites of women with abnormal cervical cytology.
Robin Crawford, Anne-Laure Grignon, Sarah Kitson, David M Winder, Siolian LR Ball, Katie Vaughan, Margaret A Stanley, Jane C Sterling, Peter KC Goon
Nov 16, 2011
Differential expression of selected histone modifier genes in human solid cancers.
Hilal Ozdağ, Andrew E Teschendorff, Ahmed Ashour Ahmed, Sarah J Hyland, Cherie Blenkiron, Linda Bobrow, Abhi Veerakumarasivam, Glynn Burtt, Tanya Subkhankulova, Mark J Arends, V Peter Collins, David Bowtell, Tony Kouzarides, James D Brenton, Carlos Caldas
Jun 14, 2011
Breast Neoplasms Carcinoma Cell Transformation Neoplastic Cluster Analysis Colorectal Neoplasms DNA Complementary DNA Neoplasm Female Gene Expression Regulation Enzymologic Gene Expression Regulation Neoplastic Histone Acetyltransferases Histone Code Histone Deacetylases Histone Methyltransferases Histone-Lysine N-Methyltransferase Histones Humans Linear Models Lung Neoplasms Male Neoplasm Proteins Neoplasms Ovarian Neoplasms Pancreatic Neoplasms Polymerase Chain Reaction Polymorphism Single-Stranded Conformational Protein Methyltransferases Protein Processing Post-Translational
Genomic analysis of heat-shock factor targets in Drosophila.
Ian Birch-Machin, Shan Gao, David Huen, Richard McGirr, Robert AH White, Steven Russell
Jun 14, 2011
Epigenetic analysis of regulatory T cells using multiplex bisulfite sequencing.
Daniel B Rainbow, Xin Yang, Oliver Burren, Marcin L Pekalski, Deborah J Smyth, Marcus DR Klarqvist, Christopher J Penkett, Kim Brugger, Howard Martin, John A Todd, Chris Wallace, Linda S Wicker
Aug 18, 2015
High fragmentation characterizes tumour-derived circulating DNA.
Florent Mouliere, Bruno Robert, Erika Arnau Peyrotte, Maguy Del Rio, Marc Ychou, Franck Molina, Celine Gongora, Alain R Thierry
May 01, 2019
BACKGROUND: Circulating DNA (ctDNA) is acknowledged as a potential diagnostic tool for various cancers including colorectal cancer, especially when considering the detection of mutations. Certainly due to lack of normalization...
Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals.
Igor Loncaric, Anna Kübber-Heiss, Annika Posautz, Werner Ruppitsch, Sarah Lepuschitz, Bernhard Schauer, Andrea T Feßler, Reinhild Krametter-Frötscher, Ewan M Harrison, Mark A Holmes, Frank Künzel, Michael P Szostak, Tomasz Hauschild, Amélie Desvars-Larrive, Dusan Misic, Renate Rosengarten, Chris Walzer, Peter Slickers, Stefan Monecke, Ralf Ehricht, Stefan Schwarz, Joachim Spergser
Mar 15, 2019
The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The...
Animals Antimicrobial resistance Coagulase-negative Staphylococcus spp. mecA mecC Animals Animals Domestic Animals Wild Anti-Bacterial Agents Bacterial Proteins Cefoxitin Coagulase DNA Bacterial Drug Resistance Multiple Bacterial Goats Lynx methicillin resistance Microbial Sensitivity Tests Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Sheep Staphylococcus
No evidence for infection of UK prostate cancer patients with XMRV, BK virus, Trichomonas vaginalis or human papilloma viruses.
The prevalence of specific infections in UK prostate cancer patients was investigated. Serum from 84 patients and 62 controls was tested for neutralisation of xenotropic murine leukaemia virus-related virus (XMRV) Envelope. No...
Antibodies Neutralizing BK Virus Cohort Studies DNA DNA Virus Infections Humans Male Papillomaviridae Polymerase Chain Reaction Polyomavirus Infections Prostatic Neoplasms Trichomonas Infections Trichomonas vaginalis Tumor Virus Infections United Kingdom Xenotropic murine leukemia virus-related virus
Molecular Criteria for Defining the Naive Human Pluripotent State.
Thorold W Theunissen, Marc Friedli, Yupeng He, Evarist Planet, Ryan C O'Neil, Styliani Markoulaki, Julien Pontis, Haoyi Wang, Alexandra Iouranova, Michaël Imbeault, Julien Duc, Malkiel A Cohen, Katherine J Wert, Rosa Castanon, Zhuzhu Zhang, Yanmei Huang, Joseph R Nery, Jesse Drotar, Tenzin Lungjangwa, Didier Trono, Joseph R Ecker, Rudolf Jaenisch
Jul 07, 2016
DNA Methylation X chromosome reactivation Embryonic Stem Cells Imprinting mouse-human chimeras Pluripotency transposable elements Animals Blastocyst Cell Differentiation Cell Line Chimera Chromosomes Human X Cleavage Stage Ovum DNA Methylation DNA Transposable Elements DNA Mitochondrial Female Gene Expression Profiling Genome Human Genomic Imprinting Human Embryonic Stem Cells Humans Male Mice Mitochondria Morula Pluripotent Stem Cells Polymerase Chain Reaction Transcription Genetic
Vibrio cholerae Serogroup O139
Fahima Chowdhury, Alison E Mather, Yasmin Ara Begum, Muhammad Asaduzzaman, Nabilah Baby, Salma Sharmin, Rajib Biswas, Muhammad Ikhtear Uddin, Regina C LaRocque, Jason B Harris, Stephen B Calderwood, Edward T Ryan, John D Clemens, Nicholas R Thomson, Firdausi Qadri
Nov 16, 2015
Role of DNA-detection-based tools for monitoring the soil-transmitted helminth treatment response in drug-efficacy trials.
Javier Gandasegui, María Martínez-Valladares, Berta Grau-Pujol, Alejandro J Krolewiecki, Rafael Balaña-Fouce, Woyneshet Gelaye, Lisette van Lieshout, Stella Kepha, Inácio Mandomando, José Muñoz, Stopping Transmission Of intestinal Parasites (STOP) project consortium
Feb 06, 2020
Spontaneous Fermentation of Malted Barley
Nathan S Fritzinger
Jan 01, 0001
Fermentation is used to preserve foods and make them taste better, such as in wines, beer, yogurt, and most cheeses. We looked at spontaneously fermented foods that contain diverse microbial communities, such as bacteria, yeast...
Fermentation sour barley mash Probiotics Polymerase Chain Reaction SEQUENCING spontaneous Biochemistry Biochemistry, Biophysics, and Structural Biology Food Chemistry Food Microbiology Life Sciences Molecular Biology
Published by: Ursinus College
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