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In organisms from all domains of life, multi-enzyme assemblies play central roles in defining transcript lifetimes and facilitating RNA-mediated regulation of gene expression. An assembly dedicated to such roles, known as the...

RNA condensates RNA metabolism RNA surveillance RNase E colloidal polymer Intrinsically Disordered Protein post-transcriptional gene regulation Ribonuclease riboregulation Catalytic Domain Cryoelectron Microscopy Electrophoretic Mobility Shift Assay Endoribonucleases Escherichia coli Escherichia coli Proteins Gene Expression Regulation Bacterial Intrinsically Disordered Proteins Models Structural Multienzyme Complexes Mutation Polyribonucleotide Nucleotidyltransferase RNA Helicases RNA processing Post-Transcriptional RNA Bacterial Ribonucleases Static Electricity Tomography

Aconitate Hydratase Archaea Bacteria Endoribonucleases Eukaryotic Cells evolution Molecular Exosomes Gene Expression Regulation Bacterial Phosphopyruvate Hydratase Polyribonucleotide Nucleotidyltransferase RNA Helicases RNA processing Post-Transcriptional RNA Bacterial

The conserved endoribonuclease RNase E dominates the dynamic landscape of RNA metabolism and underpins control mediated by small regulatory RNAs in diverse bacterial species. We explored the enzyme's hydrolytic mechanism...

RNA degradosome RNA recognition modified RNA pseudouridine ribonuclease mechanism riboregulation Binding Sites Endoribonucleases Escherichia coli Escherichia coli Proteins Multienzyme Complexes Nucleic Acid Conformation Polyribonucleotide Nucleotidyltransferase Protein Conformation pseudouridine RNA Helicases RNA Bacterial

Polynucleotide phosphorylase (PNPase) is an ancient exoribonuclease conserved in the course of evolution and is found in species as diverse as bacteria and humans. Paradoxically, Escherichia coli PNPase can act not only as an...

Hfq RNA chaperone cryoEM Gene Silencing polynucleotide phosphorylase Ribonuclease ribonucleoprotein complex riboregulation small regulatory RNA Catalytic Domain Endoribonucleases Escherichia coli Escherichia coli Proteins Exoribonucleases Gene Expression Regulation Bacterial Host Factor 1 Protein Molecular Chaperones Polyribonucleotide Nucleotidyltransferase RNA Stability RNA Bacterial RNA Small Untranslated

Bacterial Proteins Binding Sites Catalytic Domain Caulobacter crescentus DEAD-box RNA Helicases Endoribonucleases Models Molecular Multienzyme Complexes Polyribonucleotide Nucleotidyltransferase Protein Interaction Domains and Motifs RNA Helicases RNA Bacterial Ribonuclease III

Pseudomonas aeruginosa RNA degradosome RNA metabolism bacteriophage Biochemistry infectious disease microbiology protein-protein interactions Virus Binding Sites Crystallography X-Ray Endoribonucleases host-parasite interactions Models Molecular Multienzyme Complexes Polyribonucleotide Nucleotidyltransferase Protein Binding Protein Conformation Protein Multimerization Pseudomonas Phages Pseudomonas aeruginosa RNA Helicases Viral Proteins

In recent years mitochondrial DNA (mtDNA) has transitioned to greater prominence across diverse areas of biology and medicine. The recognition of mitochondria as a major biochemical hub, contributions of mitochondrial...

CRISPR/Cas9 RNA import mitoTALEN Mitochondria mtDNA mtZFN Animals Biolistics Biological Transport CRISPR-Cas Systems DNA Mitochondrial Dependovirus Endodeoxyribonucleases Gene Editing Genetic Vectors Genome Mitochondrial Mammals Mitochondria Polyribonucleotide Nucleotidyltransferase RNA Guide Kinetoplastida Transcription Activator-Like Effector Nucleases Transcription Factors

The RNA degradosome is a multi-enzyme assembly that plays a central role in the RNA metabolism of Escherichia coli and numerous other bacterial species including pathogens. At the core of the assembly is the endoribonuclease...

Binding Sites Crystallography X-Ray Endoribonucleases Escherichia coli Proteins Host Factor 1 Protein Models Molecular Multienzyme Complexes Nucleic Acid Conformation Polyribonucleotide Nucleotidyltransferase Protein Binding Protein Domains RNA Helicases RNA Bacterial