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Conserved Helix-Flanking Prolines Modulate Intrinsically Disordered Protein
Michael D Crabtree, Wade Borcherds, Anusha Poosapati, Sarah L Shammas, Gary W Daughdrill, Jane Clarke
Mar 26, 2018
Appropriate integration of cellular signals requires a delicate balance of ligand-target binding affinities. Increasing the level of residual structure in intrinsically disordered proteins (IDPs), which are overrepresented in...
Amino Acid Sequence Animals Binding Sites Cloning Molecular Conserved Sequence Escherichia coli Gene Expression Histone-Lysine N-Methyltransferase Humans Intrinsically Disordered Proteins Membrane Proteins Mice Models Molecular Mutation Myeloid-Lymphoid Leukemia Protein Phosphoproteins Proline Protein Binding Protein Conformation alpha-Helical Protein Folding Protein Interaction Domains and Motifs Recombinant Proteins Sequence Alignment Sequence Homology Amino Acid Tumor Suppressor Protein p53
Drosophila studies support a role for a presynaptic synaptotagmin mutation in a human congenital myasthenic syndrome.
Mallory C Shields, Matthew R Bowers, McKenzie M Fulcer, Madelyn K Bollig, Patrick J Rock, Bryan R Sutton, Alysia D Vrailas-Mortimer, Hanns Lochmüller, Roger G Whittaker, Rita Horvath, Noreen E Reist
Mar 07, 2019
During chemical transmission, the function of synaptic proteins must be coordinated to efficiently release neurotransmitter. Synaptotagmin 2, the Ca2+ sensor for fast, synchronized neurotransmitter release at the human...
Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis.
Double-stranded RNA (dsRNA) is a potent proinflammatory signature of viral infection. Long cytosolic dsRNA is recognized by MDA5. The cooperative assembly of MDA5 into helical filaments on dsRNA nucleates the assembly of a...
AGS ATPase Aicardi-Goutières syndrome DExD/H-box RNA helicase RIG-I-like receptor RLR SF2 helicases SMS Singleton-Merten syndrome cryo-EM Cryoelectron Microscopy helical reconstruction innate immune pattern recognition nucleic acid sensing superfamily 2 helicases Adenosine Triphosphate Cryoelectron Microscopy HEK293 Cells Humans Hydrolysis Hydrophobic and Hydrophilic Interactions Interferon-Induced Helicase IFIH1 Interferon-beta Molecular Docking Simulation Mutation Nucleic Acid Conformation Protein Conformation RNA Double-Stranded Signal Transduction Structure-Activity Relationship
Stable MOB1 interaction with Hippo/MST is not essential for development and tissue growth control.
Yavuz Kulaberoglu, Kui Lin, Maxine Holder, Zhongchao Gai, Marta Gomez, Belul Assefa Shifa, Merdiye Mavis, Lily Hoa, Ahmad AD Sharif, Celia Lujan, Ewan St John Smith, Ivana Bjedov, Nicolas Tapon, Geng Wu, Alexander Hergovich
Jul 12, 2018
The Hippo tumor suppressor pathway is essential for development and tissue growth control, encompassing a core cassette consisting of the Hippo (MST1/2), Warts (LATS1/2), and Tricornered (NDR1/2) kinases together with MOB1 as an...
DynaMut
Proteins are highly dynamic molecules, whose function is intrinsically linked to their molecular motions. Despite the pivotal role of protein dynamics, their computational simulation cost has led to most structure-based...
Functional Alleles of Chicken BG Genes, Members of the Butyrophilin Gene Family, in Peripheral T Cells.
B-G B7 family Adaptive Immunity innate immunity Membrane Protein Alleles Alternative Splicing Amino Acid Sequence Animals Butyrophilins Chickens Cloning Molecular Exons Gene Expression Haplotypes Introns Models Molecular Multigene Family Phylogeny Protein Conformation Sequence Analysis DNA Structure-Activity Relationship T-Lymphocytes
Resolving $\textit{TYK2}$ locus genotype-to-phenotype differences in autoimmunity
CA Dendrou, A Cortes, L Shipman, HG Evans, KE Attfield, L Jostins, T Barber, G Kaur, SB Kuttikkatte, OA Leach, C Desel, SL Faergeman, J Cheeseman, MJ Neville, S Sawcer, A Compston, AR Johnson, C Everett, JI Bell, F Karpe, M Ultsch, C Eigenbrot, G McVean, L Fugger
Feb 23, 2017
Animals Autoimmune Diseases Autoimmunity CD4-Positive T-Lymphocytes Cytokines Epigenesis Genetic Female Genetic Association Studies genetic variation Genomics Genotype HEK293 Cells Homozygote Humans Immune System Janus Kinase 2 Leukocytes Mononuclear Male Mice Mutation Missense Phenotype Polymorphism Single Nucleotide Protein Conformation Quantitative Trait Loci Recombination Genetic Sequence Analysis RNA Signal Transduction TYK2 Kinase Transcriptome
3D complex
Most of the proteins in a cell assemble into complexes to carry out their function. It is therefore crucial to understand the physicochemical properties as well as the evolution of interactions between proteins. The Protein Data...
Linker length affects photostability of protein-targeted sensor of cellular microviscosity.
Markéta Kubánková, Joseph E Chambers, Roland G Huber, Peter J Bond, Stefan J Marciniak, Marina K Kuimova
Sep 26, 2019
Viscosity sensitive fluorophores termed 'molecular rotors' represent a convenient and quantitative tool for measuring intracellular viscosity via Fluorescence Lifetime Imaging Microscopy (FLIM). We compare the FLIM performance...
Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 receptor via a common vestibule site.
Marijke Brams, Cedric Govaerts, Kumiko Kambara, Kerry L Price, Radovan Spurny, Anant Gharpure, Els Pardon, Genevieve L Evans, Daniel Bertrand, Sarah CR Lummis, Ryan E Hibbs, Jan Steyaert, Chris Ulens
Apr 17, 2020
Pentameric ligand-gated ion channels (pLGICs) or Cys-loop receptors are involved in fast synaptic signaling in the nervous system. Allosteric modulators bind to sites that are remote from the neurotransmitter binding site, but...
ANS complex of St John's wort PR-10 protein with 28 copies in the asymmetric unit
Joanna Sliwiak, Zbigniew Dauter, Marcin Kowiel, Airlie J McCoy, Randy J Read, Mariusz Jaskolski
May 26, 2015
Decoding Corticotropin-Releasing Factor Receptor Type 1 Crystal Structures.
Andrew S Dore, Andrea Bortolato, Kaspar Hollenstein, Robert KY Cheng, Randy J Read, Fiona H Marshall
Jun 17, 2016
Structure of human Cdc45 and implications for CMG helicase function.
Forty Years of Clathrin-coated Vesicles.
Margaret S Robinson
Oct 01, 2015
Structure-Free Validation of Residual Dipolar Coupling and Paramagnetic Relaxation Enhancement Measurements of Disordered Proteins.
Francisco N Newby, Alfonso De Simone, Maho Yagi-Utsumi, Xavier Salvatella, Christopher M Dobson, Michele Vendruscolo
Oct 21, 2015
Serine-Selective Bioconjugation.
Julien C Vantourout, Srinivasa Rao Adusumalli, Kyle W Knouse, Dillon T Flood, Antonio Ramirez, Natalia M Padial, Alena Istrate, Katarzyna Maziarz, Justine N deGruyter, Rohan R Merchant, Jennifer X Qiao, Michael A Schmidt, Michael J Deery, Martin D Eastgate, Philip E Dawson, Gonçalo JL Bernardes, Phil S Baran
Sep 25, 2020
This Communication reports the first general method for rapid, chemoselective, and modular functionalization of serine residues in native polypeptides, which uses a reagent platform based on the P(V) oxidation state. This...
Detection of translational noncrystallographic symmetry in Patterson functions.
Iracema Caballero, Massimo D Sammito, Pavel V Afonine, Isabel Usón, Randy J Read, Airlie J McCoy
Jan 11, 2021
Detection of translational noncrystallographic symmetry (TNCS) can be critical for success in crystallographic phasing, particularly when molecular-replacement models are poor or anomalous phasing information is weak. If the...
Fragment-based determination of a proteinase K structure from MicroED data using ARCIMBOLDO_SHREDDER.
Logan S Richards, Claudia Millán, Jennifer Miao, Michael W Martynowycz, Michael R Sawaya, Tamir Gonen, Rafael J Borges, Isabel Usón, Jose A Rodriguez
Feb 19, 2021
Structure determination of novel biological macromolecules by X-ray crystallography can be facilitated by the use of small structural fragments, some of only a few residues in length, as effective search models for molecular...
Identification and Characterization of Genetic Determinants of Isoniazid and Rifampicin Resistance in Mycobacterium tuberculosis in Southern India.
Asma Munir, Narender Kumar, Suresh Babu Ramalingam, Sembulingam Tamilzhalagan, Siva Kumar Shanmugam, Alangudi Natarajan Palaniappan, Dina Nair, Padma Priyadarshini, Mohan Natarajan, Srikanth Tripathy, Uma Devi Ranganathan, Sharon J Peacock, Julian Parkhill, Tom L Blundell, Sony Malhotra
Jul 03, 2019
Adult Allosteric Regulation Antitubercular Agents Bacterial Proteins Catalase DNA-Directed RNA Polymerases Drug Resistance Bacterial Humans india Isoniazid Machine Learning Models Molecular Mutation Mycobacterium tuberculosis Protein Conformation Protein Stability Rifampin Tuberculosis whole genome sequencing
Insights into POT1 structural dynamics revealed by cryo-EM.
Telomeres are protein-DNA complexes that protect the ends of linear eukaryotic chromosomes. Mammalian telomeric DNA consists of 5'-(TTAGGG)n-3' double-stranded repeats, followed by up to several hundred bases of a 3'...
SAP domain forms a flexible part of DNA aperture in Ku70/80.
Aleš Hnízda, Petr Tesina, Thanh-Binh Nguyen, Zdeněk Kukačka, Lukas Kater, Amanda K Chaplin, Roland Beckmann, David B Ascher, Petr Novák, Tom L Blundell
Jul 28, 2021
Aggregation Condition-Structure Relationship of Mouse Prion Protein Fibrils.
Jēkabs Fridmanis, Zigmantas Toleikis, Tomas Sneideris, Mantas Ziaunys, Raitis Bobrovs, Vytautas Smirnovas, Kristaps Jaudzems
Nov 25, 2021
Prion diseases are associated with conformational conversion of cellular prion protein into a misfolded pathogenic form, which resembles many properties of amyloid fibrils. The same prion protein sequence can misfold into...
aggregation conditions Amyloid fibril structure prion protein Amino Acid Sequence Amyloid Animals Carbon Isotopes Magnetic Resonance Spectroscopy Mice Microscopy Atomic Force Nitrogen Isotopes Prion Diseases Prion Proteins protein aggregation Pathological Protein Conformation spectroscopy Fourier Transform Infrared Structure-Activity Relationship
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