Search
Results: 74
De novo design of potent and selective mimics of IL-2 and IL-15.
Daniel-Adriano Silva, Shawn Yu, Umut Y Ulge, Jamie B Spangler, Kevin M Jude, Carlos Labão-Almeida, Lestat R Ali, Alfredo Quijano-Rubio, Mikel Ruterbusch, Isabel Leung, Tamara Biary, Stephanie J Crowley, Enrique Marcos, Carl D Walkey, Brian D Weitzner, Fátima Pardo-Avila, Javier Castellanos, Lauren Carter, Lance Stewart, Stanley R Riddell, Marion Pepper, Gonçalo JL Bernardes, Michael Dougan, K Christopher Garcia, David Baker
Jan 29, 2019
We describe a de novo computational approach for designing proteins that recapitulate the binding sites of natural cytokines, but are otherwise unrelated in topology or amino acid sequence. We use this strategy to design mimics...
Amino Acid Sequence Animals Binding Sites Colonic Neoplasms Computer Simulation Crystallography X-Ray Disease Models Animal Drug Design Humans Interleukin-15 Interleukin-2 Interleukin-2 Receptor alpha Subunit Melanoma Mice Models Molecular Molecular Mimicry Protein Stability Receptors Interleukin-2 Signal Transduction
DynaMut
Proteins are highly dynamic molecules, whose function is intrinsically linked to their molecular motions. Despite the pivotal role of protein dynamics, their computational simulation cost has led to most structure-based...
Mutations at protein-protein interfaces
Harry C Jubb, Arun P Pandurangan, Meghan A Turner, Bernardo Ochoa-Montaño, Tom L Blundell, David B Ascher
Jan 11, 2017
Identification and Characterization of Genetic Determinants of Isoniazid and Rifampicin Resistance in Mycobacterium tuberculosis in Southern India.
Asma Munir, Narender Kumar, Suresh Babu Ramalingam, Sembulingam Tamilzhalagan, Siva Kumar Shanmugam, Alangudi Natarajan Palaniappan, Dina Nair, Padma Priyadarshini, Mohan Natarajan, Srikanth Tripathy, Uma Devi Ranganathan, Sharon J Peacock, Julian Parkhill, Tom L Blundell, Sony Malhotra
Jul 03, 2019
Adult Allosteric Regulation Antitubercular Agents Bacterial Proteins Catalase DNA-Directed RNA Polymerases Drug Resistance Bacterial Humans india Isoniazid Machine Learning Models Molecular Mutation Mycobacterium tuberculosis Protein Conformation Protein Stability Rifampin Tuberculosis whole genome sequencing
SSBP1-Disease Update
Neringa Jurkute, Fabiana D'Esposito, Anthony G Robson, Robert DS Pitceathly, Francesca Cordeiro, F Lucy Raymond, Anthony T Moore, Michel Michaelides, Patrick Yu-Wai-Man, Andrew R Webster, Gavin Arno, Genomics England Research Consortium
Jan 28, 2022
Adolescent Amino Acid Sequence Child Preschool DNA Mitochondrial DNA-Binding Proteins Electroretinography Female Genes Recessive Genotyping Techniques Humans Male Middle Aged Mitochondrial Diseases Mitochondrial Proteins Molecular Conformation Molecular Sequence Data Mutation Missense Optic Atrophy Pedigree Penetrance Protein Stability Protein Structure Quaternary Retinal Dystrophies whole genome sequencing
The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis.
Alexis Leah Braun, Francesco Meghini, Gema Villa-Fombuena, Morgane Guermont, Elisa Fernandez-Martinez, Zhang Qian, Maria Dolores Martín-Bermudo, Acaimo González-Reyes, David Moore Glover, Yuu Kimata
Dec 08, 2022
APC/C Drosophila polo kinase centrosomes Fertility GERMLINE Alleles Anaphase-Promoting Complex-Cyclosome Animals Base Sequence Biological Transport Biomarkers Centrosome Drosophila Drosophila Proteins Gene Expression Regulation Genotype Oocytes Oogenesis Protein Serine-Threonine Kinases Protein Stability Proteolysis Sequence Deletion Ubiquitin-Conjugating Enzymes
Interrogation of the protein-protein interactions between human BRCA2 BRC repeats and RAD51 reveals atomistic determinants of affinity.
Daniel J Cole, Eeson Rajendra, Meredith Roberts-Thomson, Bryn Hardwick, Grahame J McKenzie, Mike C Payne, Ashok R Venkitaraman, Chris-Kriton Skylaris
Aug 08, 2017
The breast cancer suppressor BRCA2 controls the recombinase RAD51 in the reactions that mediate homologous DNA recombination, an essential cellular process required for the error-free repair of DNA double-stranded breaks. The...
Amino Acid Motifs Amino Acid Sequence BRCA2 Protein Fluorescence Polarization Immunoassay Humans Models Molecular Molecular Sequence Data Mutation Protein Binding Protein Interaction Domains and Motifs Protein Interaction Mapping Protein Stability Rad51 Recombinase Repetitive Sequences Amino Acid Sequence Alignment Thermodynamics
How Detergent Impacts Membrane Proteins
Vilius Kurauskas, Audrey Hessel, Peixiang Ma, Paola Lunetti, Katharina Weinhäupl, Lionel Imbert, Bernhard Brutscher, Martin S King, Rémy Sounier, Vincenza Dolce, Edmund RS Kunji, Loredana Capobianco, Christophe Chipot, François Dehez, Beate Bersch, Paul Schanda
Apr 18, 2018
Characterizing the structure of membrane proteins (MPs) generally requires extraction from their native environment, most commonly with detergents. Yet, the physicochemical properties of detergent micelles and lipid bilayers...
Regulation of Human γδ T Cells by BTN3A1 Protein Stability and ATP-Binding Cassette Transporters
DA Rhodes, Hung-Chang Chen, James C Williamson, Alfred Hill, Jack Yuan, Sam Smith, Harriet Rhodes, John Trowsdale, Paul J Lehner, Thomas Herrmann, Matthias Eberl
Jul 04, 2018
Activation of human Vγ9/Vδ2 T cells by ‘phosphoantigens’ (pAg), the microbial metabolite (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP) and the endogenous isoprenoid intermediate isopentenyl pyrophosphate (IPP)...
1 Underpinning research 1.1 Normal biological development and functioning 2.1 Biological and endogenous factors 2 Aetiology Generic Health Relevance ATP Binding Cassette Transporter 1 ATP Binding Cassette Transporter Subfamily G Member 1 ATP Binding Cassette Transporter Subfamily G Member 2 Antigen Presentation Antigens CD Butyrophilins Diphosphates HLA Antigens HeLa Cells Humans Interferon-gamma Lymphocyte Activation Neoplasm Proteins Protein Stability Receptors Antigen T-Cell gamma-delta T-Lymphocytes
An intrinsically labile α-helix abutting the BCL9-binding site of β-catenin is required for its inhibition by carnosic acid.
Marc de la Roche, Trevor J Rutherford, Deepti Gupta, Dmitry B Veprintsev, Barbara Saxty, Stefan M Freund, Mariann Bienz
Apr 03, 2019
Wnt/β-catenin signalling controls development and tissue homeostasis. Moreover, activated β-catenin can be oncogenic and, notably, drives colorectal cancer. Inhibiting oncogenic β-catenin has proven a formidable challenge. Here...
Abietanes Binding Sites Cell Line Tumor Colorectal Neoplasms Crystallography X-Ray HEK293 Cells HeLa Cells Humans Neoplasm Proteins Nuclear Magnetic Resonance Biomolecular Plant Extracts Proteasome Endopeptidase Complex Protein Stability Rosmarinus Signal Transduction Transcription Factors Wnt Proteins beta Catenin
piRNA-guided co-transcriptional silencing coopts nuclear export factors.
Martin H Fabry, Filippo Ciabrelli, Marzia Munafò, Evelyn L Eastwood, Emma Kneuss, Ilaria Falciatori, Federica A Falconio, Gregory J Hannon, Benjamin Czech
Jun 21, 2019
The PIWI-interacting RNA (piRNA) pathway is a small RNA-based immune system that controls the expression of transposons and maintains genome integrity in animal gonads. In Drosophila, piRNA-guided silencing is achieved, in part...
D. melanogaster PIWI proteins Chromosomes Gene Expression nuclear export factor piRNA pathway transcriptional gene silencing transposon control Active Transport Cell Nucleus Animals Chromatin Drosophila melanogaster Gene Silencing Nuclear Proteins Protein Binding Protein Stability RNA Small Interfering Transcription Genetic
Aberrant anaplastic lymphoma kinase activity induces a p53 and Rb-dependent senescence-like arrest in the absence of detectable p53 stabilization.
Anaplastic Lymphoma Kinase (ALK) is a receptor tyrosine kinase aberrantly expressed in a variety of tumor types, most notably in Anaplastic Large Cell Lymphoma (ALCL) where a chromosomal translocation generates the oncogenic...
Anaplastic Lymphoma Kinase Animals Cell Adhesion Cell Cycle Cell Proliferation Cell Transformation Neoplastic Cells Cultured Cellular Senescence Cyclin-Dependent Kinase Inhibitor p16 Embryo Mammalian FIBROBLASTS Humans Mice Models Biological Mutation Protein Processing Post-Translational Protein Stability Protein-Tyrosine Kinases Receptor Protein-Tyrosine Kinases Retinoblastoma Protein Transcription Genetic Tumor Suppressor Protein p53
Disorder in a two-domain neuronal Ca2+-binding protein regulates domain stability and dynamics using ligand mimicry.
Lasse Staby, Katherine R Kemplen, Amelie Stein, Michael Ploug, Jane Clarke, Karen Skriver, Pétur O Heidarsson, Birthe B Kragelund
Sep 16, 2021
EF-hand Frequenin IDP NCS-1 Order–disorder interplay Protein Folding Amino Acid Sequence Binding Sites Carrier Proteins Humans Hydrophobic and Hydrophilic Interactions Intrinsically Disordered Proteins Kinetics Ligands Models Molecular Mutation Neuronal Calcium-Sensor Proteins Neuropeptides Protein Domains Protein Stability Thermodynamics
Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents
B Bernardim, PMSD Cal, MJ Matos, BL Oliveira, N Martínez-Sáez, IS Albuquerque, E Perkins, F Corzana, ACB Burtoloso, G Jiménez-Osés, GJL Bernardes
Nov 04, 2016
Acetonitriles Acrylates Amino Acid Sequence Annexin A5 Benzoates Cross-Linking Reagents Cysteine HEK293 Cells Humans Immunoconjugates Maleimides Protein Conformation alpha-Helical Protein Conformation beta-Strand Protein Stability Receptor ErbB-2 Staining and Labeling Stereoisomerism Sulfides Trastuzumab
Lifetime imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox.
Edward Avezov, Benedict CS Cross, Gabriele S Kaminski Schierle, Mikael Winters, Heather P Harding, Eduardo Pinho Melo, Clemens F Kaminski, David Ron
Feb 12, 2020
Interfering with disulfide bond formation impedes protein folding and promotes endoplasmic reticulum (ER) stress. Due to limitations in measurement techniques, the relationships of altered thiol redox and ER stress have been...
TTC19 Plays a Husbandry Role on UQCRFS1 Turnover in the Biogenesis of Mitochondrial Respiratory Complex III.
Emanuela Bottani, Raffaele Cerutti, Michael E Harbour, Sabrina Ravaglia, Sukru Anil Dogan, Carla Giordano, Ian M Fearnley, Giulia D'Amati, Carlo Viscomi, Erika Fernandez-Vizarra, Massimo Zeviani
Jun 27, 2018
Rieske protein TTC19 UQCRFS1 complex III deficiency mitochondrial complex III mitochondrial disease mitochondrial quality control mitochondrial respiratory chain mouse model Animals Behavior Animal Disease Models Animal Electron Transport Complex III Female Genotype HeLa Cells Humans Iron-Sulfur Proteins Kinetics Male Membrane Proteins Mice Inbred C57BL Mice Knockout Mitochondria Mitochondrial Diseases Mitochondrial Proteins Motor Activity Nerve Degeneration Nervous System Phenotype Protein Binding Protein Stability Proteolysis Reactive Oxygen Species
High-Definition Analysis of Host Protein Stability during Human Cytomegalovirus Infection Reveals Antiviral Factors and Viral Evasion Mechanisms.
Katie Nightingale, Kai-Min Lin, Benjamin J Ravenhill, Colin Davies, Luis Nobre, Ceri A Fielding, Eva Ruckova, Alice Fletcher-Etherington, Lior Soday, Hester Nichols, Daniel Sugrue, Eddie CY Wang, Pablo Moreno, Yagnesh Umrania, Edward L Huttlin, Robin Antrobus, Andrew J Davison, Gavin WG Wilkinson, Richard J Stanton, Peter Tomasec, Michael P Weekes
Nov 05, 2018
host-pathogen interaction Immune Evasion innate immunity lysosome Proteasome protein degradation pulsed SILAC quantitative proteomics restriction factor tandem mass tag Cytomegalovirus Cytomegalovirus Infections DNA-Binding Proteins Humans Immune Evasion Protein Stability Proteins Proteomics Transcription Factors Viral Proteins
Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris.
Graham Whyteside, Marcos JC Alcocer, Janet R Kumita, Christopher M Dobson, Maria Lazarou, Richard J Pleass, David B Archer
Aug 19, 2020
We have investigated the relationship between the stability and secreted yield of a series of mutational variants of human lysozyme (HuL) in Pichia pastoris. We show that genes directly involved in the unfolded protein response...
Decoupling a tandem-repeat protein
The simple topology and modular architecture of tandem-repeat proteins such as tetratricopeptide repeats (TPRs) and ankyrin repeats makes them straightforward to dissect and redesign. Repeat-protein stability can be manipulated...
Screening of candidate substrates and coupling ions of transporters by thermostability shift assays.
Homa Majd, Martin S King, Shane M Palmer, Anthony C Smith, Liam Dh Elbourne, Ian T Paulsen, David Sharples, Peter Jf Henderson, Edmund RS Kunji
Nov 22, 2018
Substrates of most transport proteins have not been identified, limiting our understanding of their role in physiology and disease. Traditional identification methods use transport assays with radioactive compounds, but they are...
E. coli Microbacterium liquefaciens S. cerevisiae Tetrahymena thermophila Thermothelomyces thermophila human ion coupling molecular biophysics structural biology Substrate Specificity thermostability Adenosine Diphosphate Adenosine Triphosphate Animals Biological Assay Humans Ions Ligands Membrane Transport Proteins Mitochondria Protein Stability Reproducibility of Results Substrate Specificity Temperature Tetrahymena
Subcellular localization determines the stability and axon protective capacity of axon survival factor Nmnat2.
Axons require a constant supply of the labile axon survival factor Nmnat2 from their cell bodies to avoid spontaneous axon degeneration. Here we investigate the mechanism of fast axonal transport of Nmnat2 and its site of action...
Amino Acid Motifs Animals Axons Cell Survival Cells Cultured Exons Golgi Apparatus Half-Life Intracellular Membranes Lipoylation Mice Mice Inbred C57BL Neurons Nicotinamide-Nucleotide Adenylyltransferase Primary Cell Culture Protein Multimerization Protein Stability Protein Transport Single-Cell Analysis Time-Lapse Imaging Transport Vesicles Ubiquitination
Mitochondrial Protein Lipoylation and the 2-Oxoglutarate Dehydrogenase Complex Controls HIF1α Stability in Aerobic Conditions.
Stephen P Burr, Ana SH Costa, Guinevere L Grice, Richard T Timms, Ian T Lobb, Peter Freisinger, Roger B Dodd, Gordon Dougan, Paul J Lehner, Christian Frezza, James A Nathan
Nov 21, 2016
HARP
Sundeep Chaitanya Vedithi, Sony Malhotra, Marcin J Skwark, Asma Munir, Marta Acebrón-García-de-Eulate, Vaishali P Waman, Ali Alsulami, David B Ascher, Tom L Blundell
Nov 20, 2020
A metastable subproteome underlies inclusion formation in muscle proteinopathies.
Prajwal Ciryam, Matthew Antalek, Fernando Cid, Gian Gaetano Tartaglia, Christopher M Dobson, Anne-Katrin Guettsches, Britta Eggers, Matthias Vorgerd, Katrin Marcus, Rudolf A Kley, Richard I Morimoto, Michele Vendruscolo, Conrad C Weihl
Feb 13, 2020
Protein aggregation is a pathological feature of neurodegenerative disorders. We previously demonstrated that protein inclusions in the brain are composed of supersaturated proteins, which are abundant and aggregation-prone, and...
|
|< |
< |
1 |