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Biophysical Characterization and Catalytic Reactivity of Rubrerythrin and Symerythrin Model Proteins
Jenna Pellegrino
Jan 01, 0001
Rubrerythrins (Rbr) and symerythrins (Sym) are diiron carboxylate enzymes that exhibit ferroxidase activity and enhanced preferential peroxidase activity compared to other members of the ferritin-like superfamily (FLSF). This...
rubrerythrin symerythrin G4DFsc FLSF Protein Structure Function Biochemistry, Biophysics, and Structural Biology Life Sciences
Published by: Ursinus College
Allostery and dynamics in small G proteins.
The Ras family of small guanine nucleotide-binding proteins behave as molecular switches: they are switched off and inactive when bound to GDP but can be activated by GTP binding in response to signal transduction pathways....
How release of phosphate from mammalian F1-ATPase generates a rotary substep.
The rotation of the central stalk of F1-ATPase is driven by energy derived from the sequential binding of an ATP molecule to its three catalytic sites and the release of the products of hydrolysis. In human F1-ATPase, each 360°...
ATP synthase Mitochondria phosphate release rotary substep Adenosine Diphosphate Animals Catalytic Domain Cattle Crystallography X-Ray Humans Hydrolysis Mitochondria Models Molecular Molecular Motor Proteins Phosphates Protein Binding Protein Structure Secondary Proton-Translocating ATPases Temperature
Inhibition of the RNA polymerase III-mediated dsDNA-sensing pathway of innate immunity by vaccinia virus protein E3.
The vaccinia virus E3 protein is an important intracellular modulator of innate immunity that can be split into distinct halves. The C terminus contains a well defined dsRNA-binding domain, whereas the N terminus contains a...
Relocation of active site carboxylates in major facilitator superfamily multidrug transporter LmrP reveals plasticity in proton interactions
Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research.
Takuya Kobayashi, Nobuhiro Morone, Taku Kashiyama, Hideto Oyamada, Nagomi Kurebayashi, Takashi Murayama
May 28, 2019
BACKGROUND: Genetically encoded tag is a powerful tool for protein research. Various kinds of tags have been developed: fluorescent proteins for live-cell imaging, affinity tags for protein isolation, and epitope tags for...
Recombinant amyloid beta-peptide production by coexpression with an affibody ligand.
Bertil Macao, Wolfgang Hoyer, Anders Sandberg, Ann-Christin Brorsson, Christopher M Dobson, Torleif Härd
Jun 14, 2011
Structural insights into the mechanism of negative regulation of single-box high mobility group proteins by the acidic tail domain.
Katherine Stott, Matthew Watson, Mark J Bostock, Simon A Mortensen, Andrew Travers, Klaus D Grasser, Jean O Thomas
Apr 13, 2015
Acidic Regulatory Domain Casein Kinase 2 (CK2) Chromatin HMGB Protein Intrinsically Disordered Protein Nuclear Magnetic Resonance (NMR) Paramagnetic Relaxation Enhancement Phosphorylation Protein Kinase C Protein Kinase C (PKC) Animals Drosophila Proteins Drosophila melanogaster High Mobility Group Proteins Magnetic Resonance Spectroscopy Models Molecular Mutant Proteins Phosphorylation Plant Proteins Protein Structure Tertiary Structure-Activity Relationship Zea mays
Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
Cynthia Tallant, Erica Valentini, Oleg Fedorov, Lois Overvoorde, Fleur M Ferguson, Panagis Filippakopoulos, Dmitri I Svergun, Stefan Knapp, Alessio Ciulli
Feb 03, 2015
Structure and mechanism of a bacterial host-protein citrullinating virulence factor, Porphyromonas gingivalis peptidylarginine deiminase.
Theodoros Goulas, Danuta Mizgalska, Irene Garcia-Ferrer, Tomasz Kantyka, Tibisay Guevara, Borys Szmigielski, Aneta Sroka, Claudia Millán, Isabel Usón, Florian Veillard, Barbara Potempa, Piotr Mydel, Maria Solà, Jan Potempa, F Xavier Gomis-Rüth
Oct 19, 2020
Citrullination is a post-translational modification of higher organisms that deiminates arginines in proteins and peptides. It occurs in physiological processes but also pathologies such as multiple sclerosis, fibrosis...
Characterization of spindle checkpoint kinase Mps1 reveals domain with functional and structural similarities to tetratricopeptide repeat motifs of Bub1 and BubR1 checkpoint kinases.
Semin Lee, Philippe Thebault, Luca Freschi, Sylvie Beaufils, Tom L Blundell, Christian R Landry, Victor M Bolanos-Garcia, Sabine Elowe
Mar 12, 2021
Kinetochore targeting of the mitotic kinases Bub1, BubR1, and Mps1 has been implicated in efficient execution of their functions in the spindle checkpoint, the self-monitoring system of the eukaryotic cell cycle that ensures...
Amino Acid Motifs Animals Cattle Cell Cycle Proteins Chromosomes Human Computational Biology Enzyme Stability evolution Molecular HeLa Cells Humans Hydrogen-Ion Concentration M Phase Cell Cycle Checkpoints Mice Models Molecular Protein Multimerization Protein Serine-Threonine Kinases Protein Structure Tertiary Protein Transport Protein-Tyrosine Kinases Rats
SSBP1-Disease Update
Neringa Jurkute, Fabiana D'Esposito, Anthony G Robson, Robert DS Pitceathly, Francesca Cordeiro, F Lucy Raymond, Anthony T Moore, Michel Michaelides, Patrick Yu-Wai-Man, Andrew R Webster, Gavin Arno, Genomics England Research Consortium
Jan 28, 2022
Adolescent Amino Acid Sequence Child Preschool DNA Mitochondrial DNA-Binding Proteins Electroretinography Female Genes Recessive Genotyping Techniques Humans Male Middle Aged Mitochondrial Diseases Mitochondrial Proteins Molecular Conformation Molecular Sequence Data Mutation Missense Optic Atrophy Pedigree Penetrance Protein Stability Protein Structure Quaternary Retinal Dystrophies whole genome sequencing
Dynamic condensation of linker histone C-terminal domain regulates chromatin structure.
The basic and intrinsically disordered C-terminal domain (CTD) of the linker histone (LH) is essential for chromatin compaction. However, its conformation upon nucleosome binding and its impact on chromatin organization remain...
Co-repressor, co-activator and general transcription factor
At face value, the Sin3 histone deacetylase (HDAC) complex appears to be a prototypical co-repressor complex, that is, a multi-protein complex recruited to chromatin by DNA bound repressor proteins to facilitate local histone...
Computational design of transmembrane pores.
Chunfu Xu, Peilong Lu, Tamer M Gamal El-Din, Xue Y Pei, Matthew C Johnson, Atsuko Uyeda, Matthew J Bick, Qi Xu, Daohua Jiang, Hua Bai, Gabriella Reggiano, Yang Hsia, TJ Brunette, Jiayi Dou, Dan Ma, Eric M Lynch, Scott E Boyken, Po-Ssu Huang, Lance Stewart, Frank Dimaio, Justin M Kollman, Ben F Luisi, Tomoaki Matsuura, William A Catterall, David Baker
May 17, 2021
Transmembrane channels and pores have key roles in fundamental biological processes1 and in biotechnological applications such as DNA nanopore sequencing2-4, resulting in considerable interest in the design of pore-containing...
Cell Line Computer Simulation Cryoelectron Microscopy Crystallography X-Ray Electric Conductivity Escherichia coli Genes Synthetic Hydrazines Ion Channels Ion Transport Liposomes Models Molecular Patch-Clamp Techniques Porins Protein Engineering Protein Structure Secondary Solubility synthetic biology water
Identification of a lysosomal pathway regulating degradation of the bone morphogenetic protein receptor type II.
Hannah J Durrington, Paul D Upton, Simon Hoer, Jessica Boname, Benjamin J Dunmore, Jun Yang, Trina K Crilley, Lynn M Butler, David J Blackbourn, Gerard B Nash, Paul J Lehner, Nicholas W Morrell
Aug 28, 2020
Bone morphogenetic proteins (BMPs) are critically involved in early development and cell differentiation. In humans, dysfunction of the bone morphogenetic protein type II receptor (BMPR-II) is associated with pulmonary arterial...
Clinico-Genetic, Imaging and Molecular Delineation of COQ8A-Ataxia
Andreas Traschütz, Tommaso Schirinzi, Lucia Laugwitz, Nathan H Murray, Craig A Bingman, Selina Reich, Jan Kern, Anna Heinzmann, Gessica Vasco, Enrico Bertini, Ginevra Zanni, Alexandra Durr, Stefania Magri, Franco Taroni, Alessandro Malandrini, Jonathan Baets, Peter De Jonghe, Willem de Ridder, Matthieu Bereau, Stephanie Demuth, Christos Ganos, A Nazli Basak, Hasmet Hanagasi, Semra Hiz Kurul, Benjamin Bender, Ludger Schöls, Ute Grasshoff, Thomas Klopstock, Rita Horvath, Bart van de Warrenburg, Lydie Burglen, Christelle Rougeot, Claire Ewenczyk, Michel Koenig, Filippo M Santorelli, Mathieu Anheim, Renato P Munhoz, Tobias Haack, Felix Distelmaier, David J Pagliarini, Hélène Puccio, Matthis Synofzik
May 18, 2020
OBJECTIVE: To foster trial-readiness of coenzyme Q8A (COQ8A)-ataxia, we map the clinicogenetic, molecular, and neuroimaging spectrum of COQ8A-ataxia in a large worldwide cohort, and provide first progression data, including...
Structural and functional analysis of two small leucine-rich repeat proteoglycans, fibromodulin and chondroadherin.
Patricia Paracuellos, Sebastian Kalamajski, Arkadiusz Bonna, Dominique Bihan, Richard W Farndale, Erhard Hohenester
Jan 24, 2018
The small leucine-rich proteoglycans (SLRPs) are important regulators of extracellular matrix assembly and cell signalling. We have determined crystal structures at ~2.2Å resolution of human fibromodulin and chondroadherin, two...
Collagen Leucine-rich repeat X-ray crystallography Amino Acid Sequence Crystallography X-Ray Cystine Extracellular Matrix Proteins fibromodulin HEK293 Cells Humans Hydrophobic and Hydrophilic Interactions Models Molecular Protein Binding Protein Conformation beta-Strand Protein Interaction Domains and Motifs Protein Structure Quaternary Solutions
Inhibition of apoptosis and NF-κB activation by vaccinia protein N1 occur via distinct binding surfaces and make different contributions to virulence.
Carlos Maluquer de Motes, Samantha Cooray, Hongwei Ren, Gabriel MF Almeida, Kieran McGourty, Mohammad W Bahar, David I Stuart, Jonathan M Grimes, Stephen C Graham, Geoffrey L Smith
Aug 08, 2017
Vaccinia virus (VACV) protein N1 is an intracellular virulence factor and belongs to a family of VACV B-cell lymphoma (Bcl)-2-like proteins whose members inhibit apoptosis or activation of pro-inflammatory transcription factors...
The affinity purification and characterization of ATP synthase complexes from mitochondria.
Michael J Runswick, John V Bason, Martin G Montgomery, Graham C Robinson, Ian M Fearnley, John E Walker
Aug 11, 2017
The mitochondrial F₁-ATPase inhibitor protein, IF₁, inhibits the hydrolytic, but not the synthetic activity of the F-ATP synthase, and requires the hydrolysis of ATP to form the inhibited complex. In this complex, the α-helical...
Phenylalanine in the pore of the Erwinia ligand-gated ion channel modulates picrotoxinin potency but not receptor function.
Amino Acid Sequence Animals Bacterial Proteins Binding Sites Binding Competitive Cysteine Loop Ligand-Gated Ion Channel Receptors Erwinia Female GABA-A Receptor Antagonists Ion Channel Gating Membrane Potentials Models Molecular Molecular Sequence Data Mutation Missense Oocytes Phenylalanine Picrotoxin Protein Structure Secondary Protein Structure Tertiary Sequence Homology Amino Acid Sesterterpenes Xenopus laevis gamma-Aminobutyric Acid
TOPBP1 recruits TOP2A to ultra-fine anaphase bridges to aid in their resolution.
Ronan Broderick, Jadwiga Nieminuszczy, Andrew N Blackford, Alicja Winczura, Wojciech Niedzwiedz
May 22, 2015
Anaphase Antigens Neoplasm Carrier Proteins Cell Cycle Proteins Cell Line Tumor Centromere Chromatids Chromosomes DNA DNA Topoisomerases Type II DNA-Binding Proteins Gene Expression Regulation Gene Expression Regulation Neoplastic Green Fluorescent Proteins HEK293 Cells HeLa Cells Humans Microscopy Fluorescence Mitosis Mutation Nuclear Proteins Poly-ADP-Ribose Binding Proteins Protein Binding Protein Structure Tertiary
Peptide aptamers as new tools to modulate clathrin-mediated internalisation--inhibition of MT1-MMP internalisation.
Predicted mouse peroxisome-targeted proteins and their actual subcellular locations.
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