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Results: 17
A ChIP-chip approach reveals a novel role for transcription factor IRF1 in the DNA damage response.
IRF1 is a transcription factor that regulates key processes in the immune system and in tumour suppression. To gain further insight into IRF1's role in these processes, we searched for new target genes by performing chromatin...
Binding Sites Cell Line Tumor Cells Cultured Chromatin Immunoprecipitation Cross-Linking Reagents DNA Damage DNA Repair DNA-Binding Proteins Fanconi Anemia Complementation Group Proteins FIBROBLASTS Gene Expression Regulation Humans Interferon Regulatory Factor-1 Interferon-gamma Melphalan Oligonucleotide Array Sequence Analysis RNA Helicases RNA Interference Regulatory Elements Transcriptional Reproducibility of Results
Cost-effectiveness of Population-Based BRCA1, BRCA2, RAD51C, RAD51D, BRIP1, PALB2 Mutation Testing in Unselected General Population Women.
Ranjit Manchanda, Shreeya Patel, Vladimir S Gordeev, Antonis C Antoniou, Shantel Smith, Andrew Lee, John L Hopper, Robert J Macinnis, Clare Turnbull, Susan J Ramus, Simon A Gayther, Paul DP Pharoah, Usha Menon, Ian Jacobs, Rosa Legood
Jan 12, 2018
BACKGROUND: The cost-effectiveness of population-based panel testing for high- and moderate-penetrance ovarian cancer (OC)/breast cancer (BC) gene mutations is unknown. We evaluate the cost-effectiveness of population-based...
Adult Aged Aged 80 and over BRCA1 Protein BRCA2 Protein Breast Neoplasms Cost-Benefit Analysis DNA Mutational Analysis Decision Support Techniques Fanconi Anemia Complementation Group N Protein Fanconi Anemia Complementation Group Proteins Female Genetic Testing Humans Incidence Mammography Middle Aged Mutation Ovarian Neoplasms RNA Helicases United Kingdom United States
Multi-scale ensemble properties of the Escherichia coli RNA degradosome.
Tom Dendooven, Giulia Paris, Alexander V Shkumatov, Md Saiful Islam, Alister Burt, Marta A Kubańska, Tai Yuchen Yang, Steven W Hardwick, Ben F Luisi
Aug 20, 2021
In organisms from all domains of life, multi-enzyme assemblies play central roles in defining transcript lifetimes and facilitating RNA-mediated regulation of gene expression. An assembly dedicated to such roles, known as the...
RNA condensates RNA metabolism RNA surveillance RNase E colloidal polymer Intrinsically Disordered Protein post-transcriptional gene regulation Ribonuclease riboregulation Catalytic Domain Cryoelectron Microscopy Electrophoretic Mobility Shift Assay Endoribonucleases Escherichia coli Escherichia coli Proteins Gene Expression Regulation Bacterial Intrinsically Disordered Proteins Models Structural Multienzyme Complexes Mutation Polyribonucleotide Nucleotidyltransferase RNA Helicases RNA processing Post-Transcriptional RNA Bacterial Ribonucleases Static Electricity Tomography
RNase E and the High-Fidelity Orchestration of RNA Metabolism.
Impact of pseudouridylation, substrate fold, and degradosome organization on the endonuclease activity of RNase E.
The conserved endoribonuclease RNase E dominates the dynamic landscape of RNA metabolism and underpins control mediated by small regulatory RNAs in diverse bacterial species. We explored the enzyme's hydrolytic mechanism...
RNA degradosome RNA recognition modified RNA pseudouridine ribonuclease mechanism riboregulation Binding Sites Endoribonucleases Escherichia coli Escherichia coli Proteins Multienzyme Complexes Nucleic Acid Conformation Polyribonucleotide Nucleotidyltransferase Protein Conformation pseudouridine RNA Helicases RNA Bacterial
Genomic profiling of acute myeloid leukaemia associated with ataxia telangiectasia identifies a complex karyotype with wild-type TP53 and mutant KRAS, G3BP1 and IL7R.
Mae A Goldgraben, Eleanor Fewings, Alexey Larionov, James Scarth, James Redman, Nick Telford, Peter D Arkwright, Denise Bonney, Deepti Wilks, Samar Kulkarni, A Malcolm R Taylor, Marc D Tischkowitz, Stefan Meyer
Apr 02, 2020
Adolescent Ataxia Telangiectasia Ataxia Telangiectasia Mutated Proteins Chromosome Aberrations DNA Helicases Female Gene Expression Profiling Humans Interleukin-7 Receptor alpha Subunit Leukemia Myeloid Acute Poly-ADP-Ribose Binding Proteins Polymorphism Single Nucleotide Proto-Oncogene Proteins p21(ras) RNA Helicases RNA Recognition Motif Proteins Transcriptome Tumor Suppressor Protein p53
Norovirus infection results in eIF2α independent host translation shut-off and remodels the G3BP1 interactome evading stress granule formation.
Michèle Brocard, Valentina Iadevaia, Philipp Klein, Belinda Hall, Glenys Lewis, Jia Lu, James Burke, Margaret M Willcocks, Roy Parker, Ian G Goodfellow, Alessia Ruggieri, Nicolas Locker
Jan 31, 2020
Viral infections impose major stress on the host cell. In response, stress pathways can rapidly deploy defence mechanisms by shutting off the protein synthesis machinery and triggering the accumulation of mRNAs into stress...
Animals Caliciviridae Infections Cell Line Cytoplasmic Granules DNA Helicases Eukaryotic Initiation Factor-2 Humans Mice Mice Inbred C57BL Norovirus Poly-ADP-Ribose Binding Proteins Protein Biosynthesis RAW 264.7 Cells RNA RNA Helicases RNA Recognition Motif Proteins Signal Transduction Virus Replication
Molecular recognition of RhlB and RNase D in the Caulobacter crescentus RNA degradosome.
No evidence that protein truncating variants in BRIP1 are associated with breast cancer risk
Douglas F Easton, Fabienne Lesueur, Brennan Decker, Kyriaki Michailidou, Jun Li, Jamie Allen, Craig Luccarini, Karen A Pooley, Mitul Shah, Manjeet K Bolla, Qin Wang, Joe Dennis, Jamil Ahmad, Ella R Thompson, Francesca Damiola, Maroulio Pertesi, Catherine Voegele, Noura Mebirouk, Nivonirina Robinot, Geoffroy Durand, Nathalie Forey, Robert N Luben, Shahana Ahmed, Kristiina Aittomäki, Hoda Anton-Culver, Volker Arndt, Australian Ovarian Cancer Study Group, Caroline Baynes, Matthias W Beckman, Javier Benitez, David Van Den Berg, William J Blot, Natalia V Bogdanova, Stig E Bojesen, Hermann Brenner, Jenny Chang-Claude, Kee Seng Chia, Ji-Yeob Choi, Don M Conroy, Angela Cox, Simon S Cross, Kamila Czene, Hatef Darabi, Peter Devilee, Mikael Eriksson, Peter A Fasching, Jonine Figueroa, Henrik Flyger, Florentia Fostira, Montserrat García-Closas, Graham G Giles, Gord Glendon, Anna González-Neira, Pascal Guénel, Christopher A Haiman, Per Hall, Steven N Hart, Mikael Hartman, Maartje J Hooning, Chia-Ni Hsiung, Hidemi Ito, Anna Jakubowska, Paul A James, Esther M John, Nichola Johnson, Michael Jones, Maria Kabisch, Daehee Kang, kConFab Investigators, Veli-Matti Kosma, Vessela Kristensen, Diether Lambrechts, Na Li, Lifepool Investigators, Annika Lindblom, Jirong Long, Artitaya Lophatananon, Jan Lubinski, Arto Mannermaa, Siranoush Manoukian, Sara Margolin, Keitaro Matsuo, Alfons Meindl, Gillian Mitchell, Kenneth Muir, NBCS Investigators, Ines Nevelsteen, Ans van den Ouweland, Paolo Peterlongo, Sze Yee Phuah, Katri Pylkäs, Simone M Rowley, Suleeporn Sangrajrang, Rita K Schmutzler, Chen-Yang Shen, Xiao-Ou Shu, Melissa C Southey, Harald Surowy, Anthony Swerdlow, Soo H Teo, Rob AEM Tollenaar, Ian Tomlinson, Diana Torres, Thérèse Truong, Celine Vachon, Senno Verhoef, Michelle Wong-Brown, Wei Zheng, Ying Zheng, Heli Nevanlinna, Rodney J Scott, Irene L Andrulis, Anna H Wu, John L Hopper, Fergus J Couch, Robert Winqvist, Barbara Burwinkel, Elinor J Sawyer, Marjanka K Schmidt, Anja Rudolph, Thilo Dörk, Hiltrud Brauch, Ute Hamann, Susan L Neuhausen, Roger L Milne, Olivia Fletcher, Paul DP Pharoah, Ian G Campbell, Alison M Dunning, Florence Le Calvez-Kelm, David E Goldgar, Sean V Tavtigian, Georgia Chenevix-Trench
Sep 28, 2016
Structural elucidation of a novel mechanism for the bacteriophage-based inhibition of the RNA degradosome.
An Van den Bossche, Steven W Hardwick, Pieter-Jan Ceyssens, Hanne Hendrix, Marleen Voet, Tom Dendooven, Katarzyna J Bandyra, Marc De Maeyer, Abram Aertsen, Jean-Paul Noben, Ben F Luisi, Rob Lavigne
Sep 07, 2016
Pseudomonas aeruginosa RNA degradosome RNA metabolism bacteriophage Biochemistry infectious disease microbiology protein-protein interactions Virus Binding Sites Crystallography X-Ray Endoribonucleases host-parasite interactions Models Molecular Multienzyme Complexes Polyribonucleotide Nucleotidyltransferase Protein Binding Protein Conformation Protein Multimerization Pseudomonas Phages Pseudomonas aeruginosa RNA Helicases Viral Proteins
Feline Calicivirus Infection Disrupts Assembly of Cytoplasmic Stress Granules and Induces G3BP1 Cleavage.
Majid N Humoud, Nicole Doyle, Elizabeth Royall, Margaret M Willcocks, Frederic Sorgeloos, Frank van Kuppeveld, Lisa O Roberts, Ian G Goodfellow, Martijn A Langereis, Nicolas Locker
Nov 20, 2017
UNLABELLED: In response to stress such as virus infection, cells can stall translation by storing mRNAs away in cellular compartments called stress granules (SGs). This defense mechanism favors cell survival by limiting the use...
3C Viral Proteases Animals Caliciviridae Infections calicivirus Feline Carrier Proteins Cats Cysteine Endopeptidases Cytoplasmic Granules DNA Helicases Eukaryotic Initiation Factor-2 HeLa Cells Host-Pathogen Interactions Humans Mitogen-Activated Protein Kinases Phosphorylation Poly-ADP-Ribose Binding Proteins RNA Helicases RNA Recognition Motif Proteins Viral Proteins Virus Replication
Pathogenic germline variants in patients with features of hereditary renal cell carcinoma
Philip S Smith, Hannah West, James Whitworth, Bruce Castle, Francis H Sansbury, Anne Y Warren, Emma R Woodward, Marc Tischkowitz, Eamonn R Maher
Sep 10, 2020
Inherited renal cell carcinoma (RCC) is associated with multiple familial cancer syndromes but most individuals with features of non-syndromic inherited RCC do not harbor variants in the most commonly tested renal cancer...
Inherited predisposition renal cell carcinoma Adolescent Adult Aged BRCA1 Protein Carcinoma Renal Cell Checkpoint Kinase 2 Child Fanconi Anemia Complementation Group Proteins Female Genetic Heterogeneity Genetic Testing Germ-Line Mutation Humans Kidney Neoplasms Male Microphthalmia-Associated Transcription Factor Middle Aged RNA Helicases
Hypoxia-induced SETX links replication stress with the unfolded protein response.
Shaliny Ramachandran, Tiffany S Ma, Jon Griffin, Natalie Ng, Iosifina P Foskolou, Ming-Shih Hwang, Pedro Victori, Wei-Chen Cheng, Francesca M Buffa, Katarzyna B Leszczynska, Sherif F El-Khamisy, Natalia Gromak, Ester M Hammond
Aug 20, 2021
Tumour hypoxia is associated with poor patient prognosis and therapy resistance. A unique transcriptional response is initiated by hypoxia which includes the rapid activation of numerous transcription factors in a background of...
Activating Transcription Factor 4 Cell Death Cell Hypoxia Cell Line Tumor Cell Survival Chromatin Immunoprecipitation DNA Damage DNA Helicases Gene Expression Regulation Humans Multifunctional Enzymes Nucleic Acid Synthesis Inhibitors Oxygen R-Loop Structures RNA Helicases RNA-seq Unfolded Protein Response Up-Regulation Zinostatin eIF-2 Kinase
Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery
Marzia Munafò, Vera Manelli, Federica A Falconio, Ashley Sawle, Emma Kneuss, Evelyn L Eastwood, Jun Wen Eugene Seah, Benjamin Czech, Gregory J Hannon
May 29, 2019
The Piwi-interacting RNA (piRNA) pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires a specialized machinery that converts long singlestranded...
Human Norovirus NS3 Has RNA Helicase and Chaperoning Activities.
Teng-Feng Li, Myra Hosmillo, Hella Schwanke, Ting Shu, Zhaowei Wang, Lei Yin, Stephen Curry, Ian G Goodfellow, Xi Zhou
Mar 16, 2018
RNA-remodeling proteins, including RNA helicases and chaperones, act to remodel RNA structures and/or protein-RNA interactions and are required for all processes involving RNAs. Although many viruses encode RNA helicases and...
RNA chaperone RNA helicase Norovirus Amino Acid Sequence Cell Line Guanidine Humans Life Cycle Stages Molecular Chaperones Norovirus Nucleoside-Triphosphatase Protein Binding Protein Folding RNA Helicases RNA Viral Replicon Sequence Alignment Sequence Analysis Viral Nonstructural Proteins Virus Replication
Analysis of the natively unstructured RNA/protein-recognition core in the Escherichia coli RNA degradosome and its interactions with regulatory RNA/Hfq complexes.
Heather A Bruce, Dijun Du, Dijana Matak-Vinkovic, Katarzyna J Bandyra, R William Broadhurst, Esther Martin, Frank Sobott, Alexander V Shkumatov, Ben F Luisi
Jun 01, 2018
The RNA degradosome is a multi-enzyme assembly that plays a central role in the RNA metabolism of Escherichia coli and numerous other bacterial species including pathogens. At the core of the assembly is the endoribonuclease...
Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation.
Myra Hosmillo, Jia Lu, Michael R McAllaster, James B Eaglesham, Xinjie Wang, Edward Emmott, Patricia Domingues, Yasmin Chaudhry, Tim J Fitzmaurice, Matthew Kh Tung, Marc Dominik Panas, Gerald McInerney, Nicolas Locker, Craig B Wilen, Ian G Goodfellow
Aug 20, 2019
Knowledge of the host factors required for norovirus replication has been hindered by the challenges associated with culturing human noroviruses. We have combined proteomic analysis of the viral translation and replication...
CRISPR G3BP1 Norovirus infectious disease microbiology stress granule Translation Virus Animals Caliciviridae Infections Cell Line DNA Helicases Host-Pathogen Interactions Humans Mice Norovirus Poly-ADP-Ribose Binding Proteins Protein Biosynthesis RNA Helicases RNA Recognition Motif Proteins Viral Proteins